Morphology and Genetic Response of Two Stream Fishes (Opsariichthys bidens and Zacco platypus) to Environmental Changes in the Beijiang River by Yan ZHUANG, Xirui LI, Guoling CHAI, Zhenghui JIN, Zhaoyang YANG, Shiting HUANG, Chao LI

“…The results of this study are helpful to discover the response of stream fish to environmental change, which can evaluate the impact of global climate change on aquatic organisms, enrich the research on the mechanism of freshwater fish response to climate change, and provide a theoretical basis for the management and protection of fish resources in the Beijiang River.…”
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Emerging Resistance and Virulence Patterns in <i>Salmonella enterica</i>: Insights into Silver Nanoparticles as an Antimicrobial Strategy by Irina Gheorghe-Barbu, Ilda Czobor Barbu, Rareș-Ionuț Dragomir, Ioana Cristina Marinaș, Miruna Silvia Stan, Radu Pericleanu, Andreea Ștefania Dumbravă, Liviu-Iulian Rotaru, Simona Paraschiv, Leontina Mirela Bănică, Ionuț Pecete, Dan Oțelea, Violeta Corina Cristea, Mircea Ioan Popa, Marilena Monica Țânțu, Marius Surleac

“…Methods: In 2019, community-acquired <i>Salmonella</i> strains were collected and identified using MALDI-TOF mass spectrometry, antibiotic susceptibility profiles have been determined with the MicroScan system, and soluble virulence factors were evaluated using specific culture media, while biofilm formation was quantified in 96-well plates. …”
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Antimicrobial Resistance and Risk Factors of Canine Bacterial Skin Infections by Qian Wang, Siyu Chen, Shizhen Ma, Ying Jiao, Huiyi Hong, Siying Wang, Wei Huang, Qi An, Yu Song, Xukun Dang, Gege Zhang, Haiqin Ding, Yang Wang, Zhaofei Xia, Lu Wang, Yanli Lyu

“…Antimicrobial susceptibility was evaluated using the broth microdilution method according to CLSI guidelines. …”
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Kininogen enhances seizure susceptibility in mice possibly through bradykinin-induced modulation of calcium transients in glutamatergic and GABAergic neurons by Arijit Ghosh, Arijit Ghosh, Jiwen Huang, Jiwen Huang, Qian Wang, Qian Wang, Yanling Gong, Yanling Gong, Srishti Jain, Srishti Jain, Abhimanyu Thakur, Abhimanyu Thakur, Amiyangshu De, Amiyangshu De, Meixi Gong, Meixi Gong, Yoshie Kiyohara, Yoshie Kiyohara, Jingtai Hu, Lingyan Jin, Sharba Bandyopadhyay, Sharba Bandyopadhyay, Fang Chen, Shengtian Li

“…The effects on seizure susceptibility were evaluated using a pentylenetetrazole-induced seizure susceptibility test. …”
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Impact of myelodysplasia‐related and additional gene mutations in intensively treated patients with NPM1‐mutated AML by Sibylle Cocciardi, Maral Saadati, Nina Weiß, Daniela Späth, Silke Kapp‐Schwoerer, Isabelle Schneider, Annika Meid, Verena I. Gaidzik, Sabrina Skambraks, Walter Fiedler, Michael W. M. Kühn, Ulrich Germing, Karin T. Mayer, Michael Lübbert, Elli Papaemmanuil, Felicitas Thol, Michael Heuser, Arnold Ganser, Lars Bullinger, Axel Benner, Hartmut Döhner, Konstanze Döhner

“…ABSTRACT This study aimed to evaluate the impact of the myelodysplasia‐related gene (MRG) as well as additional gene mutations on outcomes in intensively treated patients with NPM1‐mutated (NPM1mut) AML. …”
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cGAMP promotes inner blood-retinal barrier breakdown through P2RX7-mediated transportation into microglia by Xiangyu Ge, Xingfei Zhu, Wei Liu, Mingsen Li, Zhaotian Zhang, Ming Zou, Mi Deng, Haifeng Cui, Ziqing Chen, Li Wang, Xuebin Hu, Rong Ju, Xiangcheng Tang, Xiaoyan Ding, Lili Gong

“…Specific inhibitors and P2RX7-deficienct mice were used to evaluate P2RX7’ role as a cGAMP transporter. The therapeutic effects of P2RX7 inhibitor were tested in db/db mice. …”
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Impact of tebipenem pivoxil on the intestinal microbiota and on establishment of colonization with carbapenem-resistant Klebsiella pneumoniae in mice by Maria F. Mojica, Bryan S. Hausman, Basya S. Pearlmutter, Elizabeth G. Zink, Brigid M. Wilson, Valentina Villamil, Cecilia Saiz, Graciela Mahler, Alejandro A. Vila, Naseer Sangwan, Curtis J. Donskey, Robert A. Bonomo

“…ABSTRACT Tebipenem pivoxil has potent in vitro activity against Enterobacterales pathogens, but requires combination with β-lactamase inhibitor to achieve activity against carbapenemase producers, including metallo-β-lactamases (MBLs). Herein, we evaluate the potential of tebipenem pivoxil, alone and in combination with the prodrug of the experimental MBL inhibitor CS319 (CS319-piv-SAc), to disrupt the indigenous mice microbiota of the colon and promote colonization by pathogens. …”
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Comparison of treatment regimens for unresectable stage III epidermal growth factor receptor (EGFR) mutant non-small cell lung cancer by Xin Dai, Qian Xu, Lei Sheng, Xue Zhang, Miao Huang, Song Li, Kai Huang, Jiahui Chu, Jian Wang, Jisheng Li, Yanguo Liu, Jianyuan Zhou, Shulun Nie, Lian Liu, Jing Ni

“…Large randomized clinical trials are urgently needed to explore the appropriate application sequences of TKI, radiotherapy, and chemotherapy. Registration:. …”
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High peak viraemia followed by spontaneous HIV‐1 control in women living with HIV‐1 subtype A1 in East Africa by Yifan Li, Bethany L. Dearlove, Eric Lewitus, Hongjun Bai, Shida Shangguan, Phuc Pham, Meera Bose, Eric Sanders‐Buell, Shana Howell Miller, Yvonne Rosario, Philip K. Ehrenberg, Sodsai Tovanabutra, Rasmi Thomas, Julie A. Ake, Sandhya Vasan, Leigh Anne Eller, Sorachai Nitayaphan, Lucas Maganga, Hannah Kibuuka, Fredrick K. Sawe, Merlin L. Robb, Morgane Rolland

“…We focused on 69 participants with longitudinal follow‐up and identified viraemic controllers who maintained viral loads <2000 copies/ml for over a year without treatment. We evaluated viral genetic and clinical features that are associated with viral control. …”
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Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway by Jiaxin Wen, Lingxian Yi, Lei Chen, Jinhan Xu, Yanmin Zhang, Qiheng Cheng, Hangyu Ping, Huanyu Wang, Feng Shuang, Wei Chai, Tujun Weng

“…Western blotting and real-time PCR were employed to analyze the expression changes of relevant molecules. RNA sequencing (RNA-seq) was performed to identify key genes and pathways regulated by DMOG. …”
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IQUB mutation induces radial spoke 1 deficiency causing asthenozoospermia with normal sperm morphology in humans and mice by Tingwenyi Hu, Xiangrong Tang, Tiechao Ruan, Shunhua Long, Guicen Liu, Jing Ma, Xueqi Li, Ruoxuan Zhang, Guoning Huang, Ying Shen, Tingting Lin

“…Genetic mutations were identified by whole-exome sequencing. Computer-aided sperm analysis, Papanicolaou staining, and electron microscopy were applied to evaluate the motility, morphology, and ultrastructure of spermatozoa, respectively. …”
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Cancer cell-specific PD-L1 expression is a predictor of poor outcome in patients with locally advanced oral cavity squamous cell carcinoma by Thu Nguyen, Minyu Wang, Sean Macdonald, David Goode, Sevastjan Kranz, Paul Joseph Neeson, Kevin Thia, Joseph A Trapani, Lei Qin, Simone Belobrov, David Wiesenfeld

“…Additionally, single-cell RNA sequencing (scRNA-seq) on a smaller cohort (n=10) further characterized the PD-L1hi or PD-L1lo cancer cells in these tumors.Results Elevated PD-L1 expression was associated with poor outcomes in patients with locally advanced OCSCC undergoing standard adjuvant therapy, irrespective of “hot” or “cold” classification based on TILs assessment. …”
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Therapeutic effects of medicinal and food-based traditional herbal couples on type 2 diabetes mellitus based on pharmacodynamics and pharmacokinetics by Yuhang Xu, Jing Li, Mengyao Cui, Xiaoliang Li, Hongyan Zhai, Deling Wu, Deling Wu, Deling Wu, Xiaoqin Chu, Xiaoqin Chu, Xiaoqin Chu, Xiaoqin Chu

“…The abundance of rats’ gut flora was detected by 16s rDNA sequencing, and changes in the content of short-chain fatty acids (SCFAs) in feces of rats were detected by GC-MS; and the expression of G protein-coupled receptor43 (GPR43) and glucagon-like peptide-1 (GLP-1) proteins in colonic tissues of rats were detected by Western Blot. …”
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Molecular and immunological features associated with long-term benefits in metastatic NSCLC patients undergoing immune checkpoint blockade by Pedro Rocha, Rafael Bach, Laura Masfarré, Sharia Hernandez, Nil Navarro-Gorro, Adrià Rossell, Xavier Villanueva, Mario Giner, Ignacio Sanchéz, Miguel Galindo, Raúl Del Rey-Vergara, Albert Iñañez, Beatriz Sanchéz-Espiridion, Wei Lu, Ariadna Acedo-Terrades, Pau Berenguer-Molins, Albert Sánchez-Font, Roberto Chalela, Victor Curull, Álvaro Taus, Max Hardy-Werbin, Mark Sausen, Andrew Georgiadis, James White, Jennifer B Jackson, Laura Moliner, Sergi Clavé, Beatriz Bellosillo, Ana Rovira, Ignacio Wistuba, Luisa M Solis Soto, Júlia Perera-Bel, Edurne Arriola

“…Plasma ctDNA next-generation sequencing panel (NGS) and serum proteomics were performed. …”
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Disruption of glutamine transport uncouples the NUPR1 stress-adaptation program and induces prostate cancer radiosensitivity by Uğur Kahya, Vasyl Lukiyanchuk, Ielizaveta Gorodetska, Matthias M. Weigel, Ayşe Sedef Köseer, Berke Alkan, Dragana Savic, Annett Linge, Steffen Löck, Mirko Peitzsch, Ira-Ida Skvortsova, Mechthild Krause, Anna Dubrovska

“…The level of gene expression was analyzed by RNA sequencing and RT-qPCR in the established cell lines or patient-derived tumor and adjacent non-cancerous tissues. …”
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Influence of ATXN2 intermediate CAG repeats, 9bp duplication and alternative splicing on SCA3 pathogenesis by Marilena Lauerer, Jennifer Faber, Nicolas Casadei, Magda M. Santana, Georg Auburger, Michaela Pogoda, Jakob Admard, Lea Kaupp, Patricia Laura Kos, Mafalda Raposo, Manuela Lima, Luis Pereira de Almeida, Hector Garcia-Moreno, Paola Giunti, Jeroen de Vries, Bart P. van de Warrenburg, Judith van Gaalen, Marcus Grobe-Einsler, Berkan Koyak, Kathrin Reetz, Friedrich Erdlenbruch, Heike Jacobi, Jon Infante, Holger Hengel, Ludger Schöls, Thomas Klockgether, Olaf Rieß, Jeannette Hübener-Schmid, ESMI study group

“…Fragment analyses were performed to determine the cytosine-adenine-guanine (CAG) repeat length and the 9bp duplication in ATXN2. RNA sequencing was performed on blood and cerebellum to evaluate ATXN2 isoform profile. …”
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Phosphate-solubilizing function of Pediococcus pentosaceus PSM16 and its underlying mechanism by Hong Yaling, Chen Shasha, Li Mengyao, Li Wenhui, Gao Yan, Luo Mengjiao, Zhou Qian, Zhou Siyuan, Zhou Diao, Li Xinhui, Zhang Lei, Zhou Qiong, Yang Ziqiang, Xia Yandong, Zhang Gaihua, Jia Yin

“…Through whole-genome sequencing of PSM16, we have identified key genes associated with the production of acid phosphatase. …”
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Integrating proteomics and machine learning reveals characteristics and risks of lymph node-independent distant metastasis in colorectal cancer by Chenxiao Zheng, Baiwang Zhu, Yanyu Chen, Numan Shahid, Yiwang Hu, Hajar Mansoor Ahmed Ali Husain, Binbin Ou, Qiongying Zhang, Haobo Jin, Yating Zheng, Peng Li, Yifei Pan, Xiaodong Zhang, Xiaodong Zhang

“…Tumor Mutation Burden (TMB), Microsatellite Instability (MSI) score, immune functions and immune checkpoint gene expression were analyzed to evaluate immunotherapy response. Single cell RNA sequencing (scRNA-seq) analysis illustrated the expression profile of integrin α11 (ITGA11) in CRC. …”
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High chromosomal instability is associated with higher 10‐year risks of recurrence for hormone receptor‐positive, human epidermal growth factor receptor 2‐negative breast cancer pa... by Yu‐Yang Liao, Jianfei Fu, Xiang Lu, Ziliang Qian, Yang Yu, Liang Zhu, Jia‐Ni Pan, Pu‐Chun Li, Qiao‐Yan Zhu, Xiaolin Li, Wenyong Sun, Xiao‐Jia Wang, Wen‐Ming Cao

“…Chromosomal instability (CIN) was one of the key drivers of breast cancer metastasis. Here we evaluate CIN and 10‐year invasive disease‐free survival (iDFS) and overall survival (OS) in HR+/HER2−– breast cancer. …”
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Maternal intestinal L. vaginalis facilitates embryo implantation and survival through enhancing uterine receptivity in sows by Qianhong Ye, Yifan Hu, Haoyi Jiang, Tingting Luo, Longshan Han, Yuwen Chen, Jiaying Chen, Libao Ma, Ziyi He, Xianghua Yan

“…Results In this study, we first conducted 16S rRNA sequencing and metabolomic analysis revealing the intestinal microbiota and metabolism of 42 sows with different litter size to select the potential functional microbiota that may contribute to embryo survival. …”
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