Hsa_circ_0002238 promotes the malignant behavior of colorectal cancer by Lin-Ting Zhang, Yuejia Zhou, Tiantian Wang, Boran Chen, Zhigao Cai, Shubin Wang, Gangling Tong

“…Furthermore, flow cytometry was also used to evaluate apoptosis rates in these high-expressing CRC cells. …”
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Novel PD-1-targeted, activity-optimized IL-15 mutein SOT201 acting in cis provides antitumor activity superior to PD1-IL2v by Ulrich Moebius, Radek Špíšek, David Béchard, Zuzana Antosova, Nada Podzimkova, Irena Adkins, Klara Danova, Ekaterina Simonova, Katerina Behalova, Petr Danek, Matej Fabisik, Kamila Hladikova, Lucie Kosinova, Pavel Marasek, Vladyslav Mazhara, Katerina Sajnerova, Milada Sirova, Marek Kovar, Martin Steegmaier, Hana Matuskova, Iva Malatova, Klara Hrabankova, Denise Greco, Ondrej Martinec, Romana Mikyskova, Milan Reinis, Lenka Palova Jelinkova

“…These immunocytokines spatiotemporally reinvigorate PD-1+ CD8+ tumor-infiltrating lymphocytes (TILs) via cis-activation and concomitantly activate the innate immunity via IL-2/15Rβγ signaling.Methods Human peripheral blood mononuclear cell and cell lines were used to evaluate cis/trans activity of SOT201. Anti-PD-1 mAb responsive (MC38, CT26) and resistant (B16F10, CT26 STK11 KO) mouse tumor models were used to determine the anticancer efficacy, and the underlying immune cell activity was analyzed via single-cell RNA sequencing and flow cytometry. …”
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Prophages are infrequently associated with antibiotic resistance in Pseudomonas aeruginosa clinical isolates by Tony H. Chang, Julie D. Pourtois, Naomi L. Haddock, Daisuke Furukawa, Kate E. Kelly, Derek F. Amanatullah, Elizabeth Burgener, Carlos Milla, Niaz Banaei, Paul L. Bollyky

“…However, we find that they are not encoded directly on prophage sequences. Additionally, we identify a single prophage statistically associated with ciprofloxacin resistance but do not identify any genes associated with ciprofloxacin phenotypic resistance. …”
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A decrease in functional microbiomes represented as Faecalibacterium affects immune homeostasis in long-term stable liver transplant patients by Soon Kyu Lee, JooYeon Jhun, Seung Yoon Lee, Sukjung Choi, Sun Shim Choi, Myeong Soo Park, Seon-Young Lee, Keun-Hyung Cho, A Ram Lee, Joseph Ahn, Ho Joong Choi, Young Kyoung You, Pil Soo Sung, Jeong Won Jang, Si Hyun Bae, Seung Kew Yoon, Mi-La Cho, Jong Young Choi

“…Changes in the identified functional microbiome and Treg cells in tolerant patients were evaluated. The mean time after LT of the included patients was 13.2 y. …”
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Modelling the liver’s regenerative capacity across different clinical conditions by Anh Thu Nguyen-Lefebvre, Soumita Ghosh, Cristina Baciu, Bima J. Hasjim, Sara Naimimohasses, Graziano Oldani, Elisa Pasini, Michael Brudno, Nazia Selzner, Jeffrey Wrana, Mamatha Bhat

“…Proteomic validation and human SPLiT-seq (split-pool ligation-based transcriptome sequencing) data further supported their relevance across species. …”
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HSPG2 could promote normal haematopoiesis in acute myeloid leukaemia patients after complete remission by repairing bone marrow endothelial progenitor cells by Chen‐Yuan Li, Zhen‐Kun Wang, Tong Xing, Meng‐Zhu Shen, Xin‐Yan Zhang, Dan‐Dan Chen, Yu Wang, Hao Jiang, Qian Jiang, Xiao‐Jun Huang, Yuan Kong

“…The BM EPC's functions were evaluated by apoptosis, reactive oxygen species (ROS) level, migration and tube formation assays. …”
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Predicting Disease Progression in Inoperable Localized NSCLC Patients Using ctDNA Machine Learning Model by Yuqi Wu, Canjun Li, Yin Yang, Tao Zhang, Jianyang Wang, Wanxiangfu Tang, Ningyou Li, Hua Bao, Xin Wang, Nan Bi

“…Leave‐one‐out cross‐validation (LOOCV) was performed to evaluate the models' predictive performances. Results Our cfDNA neomer profiling assay showed excellent performance in detecting patients with a high risk for disease progression. …”
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The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis by Lu Zhao, Xin Chen, Yanan Zhang, Yixuan Cen, Tingjia Zhu, Lingfang Wang, Lili Xia, Yang Li, Xiaodong Cheng, Xing Xie, Weiguo Lu, Junfen Xu

“…BaseScope and immunohistochemistry (IHC) were applied to tissue microarrays for clincial validation and ROC curve analysis evaluated the diagnostic performance of circPOLD1 in serum as a liquid biopsy marker. …”
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Dietary Solid-state-fermentation product of Bacillus velezensis T23 alleviate hepatic steatosis, oxidative stress, gut barrier damage, and microbiota dysbiosis in juvenile genetica... by Qiang Hao, Rolf Erik Olsen, Einar Ringø, Qianwen Ding, Tsegay Teame, Yuanyuan Yao, Chao Ran, Yalin Yang, Zhen Zhang, Zhigang Zhou

“…The purpose of this study was to evaluate the effects of solid-state fermentation product of autochthonous Bacillus velezensis T23 on improving hepatic steatosis, liver antioxidation capacity, gut injury and gut microbiota profile of genetically improved farmed tilapia (GIFT, Oreochromis niloticus). …”
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Serum C-C motif chemokine ligand 17 as a predictive biomarker for the progression of non-idiopathic pulmonary fibrosis interstitial lung disease by Takatoshi Enomoto, Yoshito Takeda, Yuya Shirai, Takehiro Hasegawa, Feng Zhao, Hanna Lunding, Moritz Pohl, Ryuya Edahiro, Shigeyuki Shichino, Takahiro Kawasaki, Hanako Yoshimura, Reina Hara, Saori Amiya, Makoto Yamamoto, Daisuke Nakatsubo, Satoshi Tanizaki, Mana Nakayama, Yoshimi Noda, Takayuki Niitsu, Yuichi Adachi, Mari Tone, Yuko Abe, Maiko Naito, Kentaro Masuhiro, Yujiro Naito, Takayuki Shiroyama, Kotaro Miyake, Shohei Koyama, Kiyoharu Fukushima, Kota Iwahori, Haruhiko Hirata, Izumi Nagatomo, Satoshi Nojima, Masahiro Yanagawa, Yoshikazu Inoue, Atsushi Kumanogoh

“…Methods The serum levels of 13 candidate biomarkers were prospectively measured by chemiluminescent enzyme immunoassay and the utilities for predicting ILD progression were compared in the discovery cohort (total 252 patients). Moreover, we evaluated the utility of the identified biomarker in another independent cohort (154 patients with non-IPF-ILD) and examined the dynamics of the biomarker by immunoblotting and single-cell RNA sequencing (scRNA-seq) using samples of patients and a mouse model. …”
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Extracellular vesicles derived from creeping fat stem cells promote lymphatic function and restrain inflammation of Crohn's disease by Weigang Shu, Yongheng Wang, Mengfan Chen, Xiaoli Zhu, Fangtao Wang, Chunqiu Chen, Peng Du, Alexandra Bartolomucci, Xin Su, Xiaolei Wang

“…Using high‐throughput sequencing, we demonstrated that CrF‐EVs significantly increased HLEC proliferation, migration, tube formation and CCL‐21 production in a miR‐132‐3p/RASA1/ERK1/2 axis‐dependent manner. …”
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Effect of <i>CTLA4</i> gene polymorphism on relapse probability among patients with acute leukemias after allogenic hematopoietic stem cells transplantation by D. S. Romaniuk, A. A. Khmelevskaya, M. Yu. Drokov, N. N. Popova, V. A. Vasilieva, L. A. Kuzmina, G. A. Efimov, E. N. Parovichnikova, V. G. Savchenko

“…Genotyping data was validated by Sanger’s sequencing of 22 randomly selected samples. The overall survival, the event-free survival and relapse probability were calculated using the Kaplan–Mayer method. …”
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The brainstem BBSome regulates glucose homeostasis and lean mass in a state-dependent manner by Connor Laule, Deng-Fu Guo, Yuying Zhao, Paul A. Williams, Donald A. Morgan, Younes Rouabhi, Miriam McDonough, Trevor Butler, Jon Resch, Kamal Rahmouni

“…Methods: Primary cilia were assessed by immunofluorescence and confocal imaging, and brainstem neuron transcriptomes were analyzed using single-cell RNA sequencing. Mice with Phox2b-specific deletion of Ift88 or Bbs1 were studied under control or high-fat diets. …”
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Bacillus coagulans MF-06 alleviates intestinal mucosal barrier from damage in chicks infected with Salmonella pullorum via activating the Wnt/β-catenin pathway by Li Ma, Guangming Tian, Guangming Tian, Yuejin Pu, Xuguang Qin, Yinghu Zhang, Haojie Wang, Lei You, Gaofeng Zhang, Chun Fang, Xiongyan Liang, Hongbo Wei, Lei Tan, Liren Jiang

“…Analysis through 16S rRNA sequencing revealed that the intake of Bacillus coagulans MF-06 led to a significant decrease in the relative abundance of Lachnoclostridium, Shuttleworthia, and unidentified-Eggerthellaceae (p &lt; 0.05).DiscussionCollectively, the Bacillus coagulans MF-06 may provide a protective effect against Salmonella pullorum infection in chicks by enhancing growth performance, strengthening the integrity of the intestinal mucosal barrier, and stabilizing the gut microbiota.…”
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Effects of Wolbachia removal on microbial composition and diversity in Aedes albopictus: implication of using wAlbB for discriminating irradiation-based sterile and wild males by Paerhande Dilinuer, Ming Li, Datao Lin, Yu Wu, Zhongdao Wu, Xiaoying Zheng, Dongjing Zhang

“…Abstract Background The sterile insect technique (SIT) requires distinguishing sterile from wild male mosquitoes to evaluate male qualities and maintain an appropriate release ratio for efficient population suppression. …”
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Exosomal SPRY4 from adipogenic BMSCs impairs angiogenesis via the PTPRB/TIE2/PI3K axis in Steroid-induced osteonecrosis of the femoral head by Dulei Xiang, Yuandong Zhou, Hongwei Wang, Yu chen, Haobo Bai, Zihan Wang, Siyu Tan, Dong Wu, Wenlong Yan, Xiao Huang, Tingmei Chen, Chengjie Lian, Jian Zhang

“…RNA sequencing (RNAseq) was utilized to identify potential regulatory factors within exosomes derived from adipogenic BMSCs. …”
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LLT1 overexpression renders allogeneic-NK resistance and facilitates the generation of enhanced universal CAR-T cells by Shuxian Zhu, Shiyu Zuo, Chuo Li, Xingjie You, Erlie Jiang, Xiaoming Feng, Yuechen Luo

“…We performed cytotoxicity, proliferation, and cytokine assays to evaluate the functionality of universal chimeric antigen receptor-T cell (UCAR-T) cells and conducted in vitro and in vivo assays, including allogeneic responses and RNA sequencing, to assess their resistance to allogeneic T and NK cells, anti-leukemia efficacy, and persistence in treating hematologic malignancies. …”
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Novel gene rearrangement leads to altered expression of Hornerin in sensitive and chemoresistant ovarian cancer by Ghassan M. Saed, Harvey Sharma, Jenna Dabaja, Asad Nawaz, Ayesha Alvero, Robert T. Morris, Asma Basha, Lucas Werner, Toshima Z. Parris, Khalil Helou

“…Methods: We conducted proteomic analysis (n = 252) and RNA sequencing (n = 96) on primary ovarian carcinomas to evaluate HRNR expression across different histotypes, survival outcomes, and to identify genetic variants in the HRNR gene. …”
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Hepatocellular carcinoma hosts cholinergic neural cells and tumoral hepatocytes harboring targetable muscarinic receptors by Charlotte A. Hernandez, Claire Verzeroli, Armando Andres Roca Suarez, Abud-José Farca-Luna, Laurie Tonon, Roger Esteban-Fabró, Roser Pinyol, Marie-Laure Plissonnier, Ievgeniia Chicherova, Anaëlle Dubois, Pascale Bellaud, Marine Seffals, Bruno Turlin, Alain Fautrel, Gabriel Ichim, Michel Rivoire, Guillaume Passot, Zuzana Macek-Jilkova, Thomas Decaens, Alain Viari, Barbara Testoni, Sandra Rebouissou, Josep M. Llovet, Fabien Zoulim, Romain Parent

“…Methods: We characterized the innervation of liver tumors from the French Liver Biobank, then applied bioinformatics to TCGA (The Cancer Genome Atlas), several other datasets and a European validation cohort, to re-evaluate patient stratification. Cell biology and pharmacology studies were also performed. …”
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Myeloid TGF-β signaling shapes liver macrophage heterogeneity and metabolic liver disease pathogenesis by Ziyi Meng, Xiaoxue Qiu, Zhimin Chen, Yu-tung Lee, Linkang Zhou, You Lu, Tongyu Liu, Siming Li, Benjamin Levi, Katherine A. Gallagher, Jiandie D. Lin

“…Macrophage population dynamics were evaluated by flow cytometry and immunofluorescence. …”
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