Oral-gut axis in inflammation: periodontitis exacerbates ulcerative colitis via microbial dysbiosis and barrier disruption by Jinping Yu, Jinglu Lyu, Tongxin Zhu, Yang Li, Hanping Xia, Qing Liu, Lili Li, Bin Chen

“…Gut microbiota and periodontal microbiota was analyzed using 16S rRNA sequencing. Tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) were measured by quantitative PCR (qPCR) to evaluate the systemic inflammation burden. …”
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Characterization of a novel lytic phage vB_AbaM_AB4P2 encoding depolymerase and its application in eliminating biofilms formed by Acinetobacter baumannii by Jianhui Su, Yujing Tan, Shenshen Liu, Huanhuan Zou, Xiaoyi Huang, Siyi Chen, Hongmei Zhang, Shaoting Li, Haiyan Zeng

“…The morphological, host range, one-step growth, temperature and pH stability, bactericidal activity, sequencing and genomic analysis were performed to characterize the isolated phage. …”
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Waterborne Transmission Driving the Prevalence of <i>Blastocystis</i> sp. in Los Ríos Region, Southern Chile by Daniel Sanhueza Teneo, Cedric B. Chesnais, Javiera Manzano, María Paz Moll, Analía Téllez, Guillermo Valenzuela-Nieto

“…Water samples were analyzed using conventional PCR, next-generation sequencing (NGS) was employed for <i>Blastocystis</i> sp. subtype identification, and the environmental predictors were evaluated using a multivariable logistic regression model. …”
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CircPRMT5, a Potential Salivary Biomarker, Facilitates the Progression of Head and Neck Squamous Cell Carcinoma via the IGF2BP3-SERPINE1 Pathway by Jiang S, Ou L, Wang Y, Su K, Chen Z, He L, Xu X, Cheng B, Xia J, Fan Z

“…To evaluate and validate these mechanisms, Western blotting, RNA-FISH, immunofluorescence, immunohistochemistry, RIP, and rescue experiments were employed. …”
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Fecal microbiome analysis in patients with metabolic syndrome and type 2 diabetes by Laura Isabel Sinisterra Loaiza, Diego Fernández-Edreira, Jose Liñares-Blanco, Alberto Cepeda, Alejandra Cardelle-Cobas, Carlos Fernandez-Lozano

“…To analyze the microbiota composition, amplicon sequencing of 16S rRNA genes was performed on stool samples. …”
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Limosilactobacillus reuteri prevents progression of ankylosing spondylitis in mice by restoring gut microbiota-metabolism homeostasis by Lianjun Yang, Ke You, Kun Wang, Bin Liu, Tao Chen, Zhifei Cui, Dawei Zhang, Zhihai Su, Xiang Liu, Hai Lu

“…Gut microbiota (16S rRNA sequencing) and fecal metabolites (untargeted metabolomics) were analyzed. …”
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Synergistic effects of human umbilical cord mesenchymal stem cells/neural stem cells and epidural electrical stimulation on spinal cord injury rehabilitation by Zhiping Mu, Jiaodi Qin, Xiaohua Zhou, Kunzheng Wang

“…In this study, we first examined neural stem cell changes via mRNA sequencing in an in vitro Transwell co-culture model. …”
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Preliminary validation of a web-based MRI scoring system for children with chronic nonbacterial osteomyelitis (ChRonic nonbacterial Osteomyelitis Magnetic Resonance Imaging Scoring... by Farzana Nuruzzaman, T. Shawn Sato, Jennifer Stimec, Ramesh S. Iyer, Andrew Carbert, Joel Paschke, Lauren Potts, Meinrad Beer, Ming Huang, Johanna Monsalve, Anh-Vu Ngo, Mahesh Thapa, Xiaoyue Zhang, Walter P. Maksymowych, Polly J. Ferguson, Yongdong Zhao, For the CARRA CRMO Workgroup

“…The sum of these parameters for lesions detected on fluid-sensitive sequences was the CROMIS Activity Index (maximum score 720). …”
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Modulation of Gut Microbiota through Nutritional Interventions in Behçet’s Syndrome Patients: Preliminary Results from the MAMBA Study by Giuditta Pagliai, Silvia Turroni, Federica D’Amico, Irene Mattioli, Marta Tristan Asensi, Giacomo Emmi, Francesco Sofi

“…Diet is known to influence the GM, but to date no study has investigated its effect on these patients. Aim. To evaluate the effect of a lacto-ovo vegetarian diet (VD) and a Mediterranean diet supplemented with 2 g/die of oral butyrate (MD-Bt) in comparison with a Mediterranean diet (MD) on the GM in BS. …”
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B cell c-Maf signaling promotes tumor progression in animal models of pancreatic cancer and melanoma by Hong Li, Shu Li, Jun Yan, Yongling Ning, Hongying Hao, Qian Zhong, Chuanlin Ding, Xiaoling Hu, Kelly M McMasters

“…Tumor progression was evaluated. B cell and T cell phenotypes were determined by flow cytometry, mass cytometry, and cytokine/chemokine profiling. …”
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Multiplex-PCR Detection of an Atypical <i>Leuconostoc mesenteroides</i> subsp. <i>jonggajibkimchii</i> Phenotype Dominating the Terminal Spoilage Microbial Association of a Fresh G... by Nikoletta Sameli, Eleni Sioziou, Loulouda Bosnea, Spiros Paramithiotis, John Samelis

“…A species-specific multiplex-PCR method and phenotypic tests were combined to evaluate biochemical and genotypic differences between 24 representative <i>Leuconostoc mesenteroides</i> diverse isolates previously found to dominate in spoiled, vacuum-packed Anthotyros whey cheeses stored at 4 °C for 40 days and identified by 16S rRNA gene sequencing. …”
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Genomic and clinical characterization of HER2 exon 20 mutations in non-small cell lung cancer: insights from a multicenter study in South China by Yating Hou, Xingyang Xue, Zhuoyun Zhang, Dahai Mai, Wei Luo, Mingyu Zhou, Zichuan Liu, Yisheng Huang

“…Progression-free survival (PFS) and overall survival (OS) were evaluated using Kaplan-Meier methods and compared with log-rank tests. …”
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Therapeutic application of a jumbo bacteriophage against metallo-β-lactamase producing Pseudomonas aeruginosa clinical isolates by Paschalis Paranos, Dimitrios Skliros, Nikita Zrelovs, Panagiota-Christina Georgiou, Karina Svanberga, Andris Kazaks, Marios Kostakis, Nikolaos Thomaidis, Emmanouil Flemetakis, Joseph Meletiadis

“…A jumbo phage isolated from wastewater in Greece was characterized microbiologically and genetically and evaluated for its potential as a therapeutic agent alone or in combination with antibiotics in an experimental thigh infection mouse model. …”
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Response gene to complement 32 promotes tumorigenesis by mediating DNA damage repair and inhibits CD8+ T cells infiltration in diffuse large B-cell lymphoma by Xiyuan Zhang, Xiyuan Zhang, Tiange Lu, Chunlei Shi, Mengfei Ding, Ling Wang, Xinting Hu, Xin Wang, Xin Wang

“…RNA-sequencing (RNA-seq) analysis was performed between shRGC32 and shControl stably transfected OCI-LY1 cells and functional enrichment analyses used gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG). …”
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Revisiting the emerging pathosystem of rice sheath blight: deciphering the Rhizoctonia solani virulence, host range, and rice genotype-based resistance by Zeinab A. Kalboush, Yasser S. A. Mazrou, Amr A. Hassan, Ahmed Sherif, Wael E. Gabr, Qurban Ali, Yasser Nehela

“…Additionally, rice genotype-based resistance was evaluated using 11 rice genotypes for their response to R. solani isolates, morphological traits, yield components, and using 12 SSR markers linked to sheath blight resistance. …”
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Virus-specific host responses and gene signatures following infection with major SARS-CoV-2 variants of concern: role of ZBP1 in viral clearance and lung inflammation by Amany Elsharkawy, Amany Elsharkawy, Hamid Reza Jahantigh, Anchala Guglani, Shannon Stone, Komal Arora, Mukesh Kumar, Mukesh Kumar

“…New variants of concern (VOCs) have raised additional concerns due to disparate pathogenicity and possible enhanced virulence. Herein, using RNA sequencing, we performed a comparative transcriptomic analysis following infection with major VOCs. …”
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Integrative profiling of lung cancer biomarkers EGFR, ALK, KRAS, and PD-1 with emphasis on nanomaterials-assisted immunomodulation and targeted therapy by Li Peng, Hongmei Li, Wencai Yan, Yingjie Liu, Haihao Zhu, Yingcai Hong

“…Molecular docking using SwissDock evaluated the binding affinities of established inhibitors. …”
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Computed tomography radiomics to predict microsatellite instability status and immunotherapy response in gastric cancer by Zhou Li, Zixuan Ding, Yongping Lian, Yongqing Liu, Lei Wang, Pengbo Hu, Fangyuan Zhang, Yan Luo, Hong Qiu

“…The preoperative contrast-enhanced CT images of GC were evaluated. Radiomics features were extracted and selected to construct the radiomics model in the training set, and further validated in the other two external testing sets. …”
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TMAO Induces Vascular Endothelial Cells Pyroptosis Through TET2-CYTB-ROS Pathway by Xia L, Wang Z, Chen X

“…CYTB methylation was detected using Targeted Bisulfite Sequencing.Results: This study showed that TMAO can down-regulate the expression of CYTB inVECs, cause VECs pyroptosis and mitochondrial dysfunction (MDF). …”
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TNFR2 blockade promotes antitumoral immune response in PDAC by targeting activated Treg and reducing T cell exhaustion by Eliane Piaggio, Allan Thiolat, Anais Debesset, Caroline Pilon, Benoît Laurent Salomon, José Laurent Cohen, Sylvain Baulande, Sylvain Meunier, Orianne Cuelenaere-Bonizec, Wilfrid Richer, Claire Houppe, Matteo Ponzo, Jeanne Magnan, Jonathan Caron, Pamela Caudana, Jimena Tosello Boari, Nhu Han To, Ilaria Cascone

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