Integration and functionality of human iPSC-derived microglia in a chimeric mouse retinal model by Chun Tang, Qi-Qi Zhou, Xiu-Feng Huang, Ya-Yi Ju, Bi-Lin Rao, Zhi-Cong Liu, Yi-An Jia, Zhan-Pei Bai, Qing-Yang Lin, Lin Liu, Jia Qu, Jun Zhang, Mei-Ling Gao

“…The chimeric model was stimulated with LPS, and single-cell RNA sequencing (scRNA-seq) was used to analyze transcriptomic changes in iMG and xMG. …”
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Programming virulent bacteriophages by developing a multiplex genome engineering method by Hailin Zhang, Ru Zhu, Zhaofei Wang, Ruoting He, Yuran Zhang, Ji Luan, Yaxian Yan, Youming Zhang, Hailong Wang

“…We developed a SMART (splitting, modifying, assembling, and rebooting) method for multiplex genome engineering of virulent phages. We deleted 3.9 kb sequences distributed across 8 sites in the 39.9 kb genome of the wild-type T7 Escherichia coli phage to construct a chassis phage. …”
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Transcriptome and hormonal analyses reveal an important role of auxin and cytokinin in regulating rice ratooning ability by Can Hu, Chenfan Zheng, Bo Liu, Like Sun, Jiaxi Yin, Yuanyuan Tan, Pedro García-Caparros, Qingyao Shu, Meng Jiang

“…In the present study, the RA trait was evaluated in 23 rice varieties cultivated across three different locations in Hainan Province, showing substantial phenotypic variation. …”
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Elucidating the gut microbiota-driven crosstalk: mechanistic interplay of lobetyolin in coordinating cholesterol homeostasis and anti-inflammatory pathways in hyperlipidemic mice m... by Guofeng Duan, Guofeng Duan, Yuning Zhang, Siyuan Liu, Siqi Wang, Jinjia Liu, Lijuan Li, Lijuan Li, Lina Lai, Lina Lai

“…This study aimed to measure lipid metabolism in mouse serum and liver, assess the inflammatory status of the mouse colon, and evaluate changes in the gut microbiota.ResultsLobetyolin significantly reduced the levels of triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), very low-density lipoprotein cholesterol (VLDL-C), and total cholesterol (T-CHO) in the serum of hyperlipidemic mice. …”
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TGFB1/CXCL5 axis regulation by LCN2 overexpression: a promising strategy to inhibit colorectal cancer metastasis and enhance prognosis by Xiaotian Song, Xiaotian Song, Shuai Xu, Shuai Xu, Dan Song, Juan Wang, Bin Bai, Yanxin An, Bin Yang, Shiqi Wang, Qingchuan Zhao, Pengfei Yu

“…RNA sequencing and proteome analysis were employed to identify potential downstream targets of LCN2. …”
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Gut microbiota metabolites, secretory immunoglobulin A and Bayley-III cognitive scores in children from the CHILD Cohort Study by Aline Davias, Myah Verghese, Sarah L. Bridgman, Hein M. Tun, Catherine J. Field, Matthew Hicks, Jacqueline Pei, Anne Hicks, Theo J. Moraes, Elinor Simons, Stuart E. Turvey, Padmaja Subbarao, James A. Scott, Piushkumar J. Mandhane, Anita L. Kozyrskyj

“…Bayley-III Scale of Infant Development was assessed at 12 and 24 months. We evaluated direct relationships in multiple linear regression models and putative causal relationships in statistical mediation models. …”
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Genomic Insights of Wheat Root-Associated <i>Lysinibacillus fusiformis</i> Reveal Its Related Functional Traits for Bioremediation of Soil Contaminated with Petroleum Products by Roderic Gilles Claret Diabankana, Akerke Altaikyzy Zhamalbekova, Aigerim Erbolkyzy Shakirova, Valeriia Igorevna Vasiuk, Maria Nikolaevna Filimonova, Shamil Zavdatovich Validov, Radik Ilyasovich Safin, Daniel Mawuena Afordanyi

“…We sequenced the whole genome of MGMM7 and provided insights into the genomic structure of related strains isolated from contaminated sources. …”
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HBV-induced N6 methyladenosine modification of PARP1 enhanced AFB1-related DNA damage and synergistically contribute to HCC by Xin Zhou, Tianman Li, Haixiang Xie, Huasheng Huang, Kejian Yang, Xiaoyun Zeng, Tao Peng

“…Sanger sequencing was employed to detect TP53 R249S mutations. …”
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Cardiovascular magnetic resonance feature tracking derived strain analysis can predict return to training following exertional heatstroke by Jun Zhang, Song Luo, Li Qi, Shutian Xu, Dongna Yi, Yue Jiang, Xiang Kong, Tongyuan Liu, Weiqiang Dou, Jun Cai, Long Jiang Zhang

“…Methods: Trained soldiers (participants) with EHS underwent CMR cine sequences between June 2020 and August 2023. Two-dimensional (2D) LV strain parameters were derived. …”
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Toripalimab plus axitinib in patients with metastatic mucosal melanoma: 3-year survival update and biomarker analysis by Xuan Wang, Jie Dai, Li Zhou, Hui Feng, Jun Guo, Xue Bai, Lu Si, Bixia Tang, Chuanliang Cui, Xinan Sheng, Siming Li, Zhihong Chi, Lili Mao, Bin Lian, Xieqiao Yan, Yan Kong, Xiaowen Wu, Xiongwen Tang, Keith T Flaherty

“…Tumor programmed cell death ligand-1 (PD-L1) expression, tumor mutational burden (TMB), and gene expression profile (GEP) by messenger RNA sequencing were evaluated for correlation with survival.Results As of April 2, 2021, the median follow-up was 42.5 months. …”
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Exosomal miR-202-5p derived from iPSC-MSCs protects against myocardial infarction through inhibition of cardiomyocyte pyroptosis by Jiaqi Chen, Xiaoting Liang, Qian Han, Haiwei He, Xinran Huang, Ying Shen, Jie Qiu, Fang Lin, Cong Mai, Ziqi Li, Kexin Ma, Bei Hu, Xin Li, Yuelin Zhang

“…Myocardial pyroptosis was evaluated using TUNEL staining and measurement of associated factors. …”
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Isolation and identification of Fusarium species from the water systems of ICUs and transplant wards of hospitals and determination of the in vitro susceptibilities of isolates to... by Fatemeh Mirhasani, Roshanak Daie-Ghazvini, Seyed Jamal Hashemi, Sadegh Khodavaisy, Pegah Ardi, Zahra Rafat, Davoud Roostaei, Heidar Bakhshi, Fatemeh Amirzadeh-Ghasemi

“…Swab samples were cultured, and the open-plate samples were evaluated for the growth of Fusarium species. The validation of all Fusarium sp. isolates was performed using DNA sequencing of the translation elongation factor 1-alpha (TEF-1α) gene. …”
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Glycan dysregulation as one of major metabolic subtypes is associated with TERC overexpression and poor outcomes in cervical cancer by Yanlei Dong, Xinyuan Zhang, Jingjie Zhao, Qingzhen Hou, Yunhai Yu, Yu Wu, Xing Shi, Lina Wang, Dawei Xu

“…The reproducibility of the classification system was further evaluated in GSE68339 CC cohort. The association of metabolic groups with clinical characteristics, telomere maintenance and somatic alterations was assessed to define molecular features of each subtype. …”
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A comprehensive analysis of the tryptophan metabolism-related gene signature to predict the prognosis of esophageal squamous cell carcinoma based on multi-omics by Zhengjie Wu, Zhiping Liu, Yukun Wang, Geling Teng, Xiaodong Li, Tong Lu, Fangning Hu, Shuo Wu, Guanqiang Ma, Hua Zhang

“…We aimed to explore the role and mechanism of Trp metabolism in ESCC.MethodsWe integrated single-cell RNA (scRNA) sequencing, bulk transcriptome, proteomics, and microbiome data from public databases. …”
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Multiparity induces persistent myocardial structural, functional and transcriptomic remodelling in mice by Ruth R. Magaye, Bing H. Wang, Hongyi R. Liu, Shanae Bailey, Rithy Nuon, Sabrina Sutano, Michael Nyugen, Helen Kiriazis, Kyah Grigolon, Luchen Shan, William W. H. Ho, Fumihiko Takeuchi, Daniel G. Donner, David M. Kaye

“…Multiparous (MP) mice (C57Bl/6J) aged 24 months were compared to age-matched non-parous (NP) mice (n = 8) in a blinded analysis. We evaluated blood pressure, body composition, gross weight and histologic cardiac structure. …”
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Successful implementation of a risk assessment and mitigation program to control bovine digital dermatitis at the herd-level by Jim Weber, Marina Hillen, Torsten Seuberlich, Andreas Fürmann, Corinne Gurtner, Jens Becker, Claudia Syring, Maria Welham Ruiters, Maher Alsaaod, Lina Mazurek, Gertraud Schüpbach, Adrian Steiner

“…Abstract This nonrandomized clinical intervention study was designed as a prospective, multicenter group comparison to evaluate the efficacy of a risk assessment and mitigation program to control bovine digital dermatitis (BDD). …”
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Prognostic implications of HIF-1α expression in anal squamous cell carcinoma treated with intensity-modulated radiotherapy (IMRT) by Ahmed Allam Mohamed, Michael J. Eble, Edgar Dahl, Danny Jonigk, Svetlana Warkentin

“…This study aimed to evaluate the prognostic significance of HIF-1α expression in patients with anal squamous cell carcinoma (ASCC) undergoing chemoradiation therapy. …”
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Metagenomic insights into the effects of Chive seed flavonoid on intestinal fermentation, morphology, and microbiota composition in sheep by Xujie Li, Xujie Li, Dengpan Li, Dengpan Li, Chunhui Wang, Chunhui Wang, Qiao Li, Qiao Li, Yajing Nie, Yajing Nie, Liming Zhang, Liming Zhang, Jin Xu, Jin Xu, Youji Ma, Youji Ma

“…This study systematically evaluated CSF supplementation (0.25% of concentrate) on intestinal morphology, volatile fatty acids (VFAs) dynamics, and microbiota composition in 16 weaned Hu lambs (3 months; 19.57 ± 1.56 kg initial weight) using histomorphometry, VFA quantification, and full-length 16S rRNA sequencing. …”
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Methylation and gene expression patterns in adamantinomatous craniopharyngioma highlight a panel of genes associated with disease progression-free survival by Junier Marrero-Gutiérrez, Ana Carolina Bueno, Ana Carolina Bueno, Clarissa Silva Martins, Rui Milton Patrício Silva-Júnior, Antônio Carlos dos Santos, Marcelo Volpon Santos, Luiz Eduardo Wildemberg, Ximene Lima da Silva Antunes, Monica R. Gadelha, Ayrton Custodio Moreira, Ricardo Zorzetto Nicoliello Vêncio, Sonir Roberto R. Antonini, Margaret de Castro

“…IntroductionOver the past decade, advancements in next-generation sequencing have significantly enhanced our understanding of the molecular pathogenesis of adamantinomatous craniopharyngiomas (ACP).ObjectiveThis study integrated methylome and transcriptome analyses in ACP samples to explore the potential interplay between DNA methylation and RNA expression signatures for diagnostic and prognostic applications in ACP patients.MethodsThis cross-sectional study evaluated clinicopathological features, DNA methylation, and gene expression profiles in 15 patients with ACP (33% women, age range: 3–55 years, 53% diagnosed before 18 years) treated at Ribeirao Preto Medical School, University of São Paulo.ResultsMultidimensional scaling and principal component analysis identified two distinct clusters (ACP-A: n=9, ACP-B: n=6) with consistent composition across DNA methylation and gene expression profiles. …”
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In Silico Design and Characterization of a Multiepitope Vaccine Candidate Against Brucella canis Using a Reverse Vaccinology Approach by Vicente Arriagada, Alberto Osorio, Crisleri Carrera-Naipil, Carlos A. Villacis-Aguirre, Cristian Escobar, Nicolás Morales, Danthe Villa, Lien Mardones, Dafne Pérez, Macarena Jara, Raúl E. Molina, Ítalo Ferrari, Sebastián Azocar, Leonardo A. Gómez, Ángel A. Oñate

“…Epitopes for B and T cells within these proteins were predicted and analyzed, selecting the most immunogenic sequences. The overlap between B- and T-cell epitopes narrowed the selection to six final epitopes. …”
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