Housed feeding improves rumen health by influencing the composition of the microbiota in Honghe cattle by Runqi Fu, Runqi Fu, Lin Han, Lin Han, Chunjia Jin, Chunjia Jin, Ye Yu, Ye Yu, Binlong Fu, Binlong Fu, Qian Li, Qian Li, Jing Leng, Jing Leng

“…The indicators of immunity and antioxidants in serum and rumen epithelium of cattle were measured; and the rumen microbiota were evaluated by using 16S rRNA and ITS sequencing techniques. …”
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Multiomics comparative analysis of feces AMRGs of Duroc pigs and Tibetan and the effect of fecal microbiota transplantation on AMRGs upon antibiotic exposure by Tao Wang, Yuheng Luo, Xiangfeng Kong, Ling Fang, Liping Zhu, Bing Yu, Ping Zheng, Zhiqing Huang, Xiangbing Mao, Yu Jie, Junqiu Luo, Hui Yan, Jun He

“…This underscores the significance of evaluating the presence of AMRGs within donor fecal microbiota for effective AMRGs decolonization strategies.…”
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Distribution and extended triage of high-risk human papillomavirus genotyping based on vaginal self-sampling by Ling Li, Yun Zhao, Rong Liu, Chen Yao, Zhijun Zhang, Yingying Hu, Ruifang Wu, Ruifang An, Hui Du, Xinfeng Qu, Lihui Wei, Bei Lin, Mingzhu Li, Chao Zhao, Jingran Li, Zhixin Lin, Jihong Deng, Songling Zhang

“…Self-collected vaginal samples were tested for 14 HR-HPV types using next-generation sequencing or multiplex real-time PCR assays.Results The five most common types were HPV52, 58, 16, 39 and 51. …”
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Immune inflammatory regulation in Anti-NMDAR encephalitis: insights from transcriptome analysis by Shan Qiao, Shan Qiao, Jia Wang, Shan-chao Zhang, Ai-hua Wang, Hai-yun Li, Tao Xin, Tao Xin

“…The Illumina HiSeq platform was used to do transcriptome sequencing. We utilized R software to identify differentially expressed genes (DEGs) and performed a functional enrichment analysis. …”
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Combining multi-omics analysis with machine learning to uncover novel molecular subtypes, prognostic markers, and insights into immunotherapy for melanoma by Songyun Zhao, Zihao Li, Kaibo Liu, Gaoyi Wang, Quanqiang Wang, Hua Yu, Wanying Chen, Hao Dai, Yijun Li, Jiaheng Xie, Yucang He, Liqun Li

“…Patients in the low MLDS group may be more responsive to chemotherapeutic agents and more likely to benefit from immune checkpoint inhibitors (ICIs). Single-cell sequencing analysis revealed that the MAPK signaling pathway between AGPAT2 + melanoma cells and fibroblasts/myeloid cells promotes tumor survival in the TME. …”
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An immunoinformatics approach in designing high-coverage mRNA multi-epitope vaccine against multivariant SARS-CoV-2 by Ernawati Arifin Giri-Rachman, Al Mirahma Febri Kurnianti, Rizarullah, Aditya Hanung Setyadi, Anita Artarini, Marselina Irasonia Tan, Catur Riani, Dessy Natalia, Reza Aditama, Husna Nugrahapraja

“…Methods: To design a multivariant vaccine, 20,567 sequences consisting of all SARS-CoV-2′s variants of concern whole genome were retrieved. …”
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SRSF3 and hnRNP A1-mediated m6A-modified circCDK14 regulates intramuscular fat deposition by acting as miR-4492-z sponge by Chunyu Qin, Fang Xu, Binglin Yue, Jincheng Zhong, Zhixin Chai, Hui Wang

“…In the present study, a novel circRNA, circCDK14 (originating from the yak’s CDK14 gene), was identified by sequencing and RNase R treatment. In our previous study, we successfully established a ceRNA network map and identified miR-4492-z, which interacts with circCDK14. …”
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Chemotherapy reprograms miRNA expression profiles in apoptotic extracellular vesicles from medulloblastoma cells, regulating pro- and anti-proliferative effects on recipient drug-n... by Rosa Mistica C. Ignacio, Helen Forgham, Zerong Ma, Anya Jensen, George Sharbeen, Juanfang Ruan, David S. Ziegler, Maria Tsoli, Phoebe A. Phillips, Chelsea Mayoh, Maria Kavallaris, Joshua McCarroll

“…EV-miRNAs were analyzed using small RNA sequencing and qPCR, and the effects of candidate miRNA overexpression on medulloblastoma cell growth and apoptosis were evaluated. …”
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Development and validation of a 10-gene signature for predicting recurrence risk in HR+/HER2- early breast cancer undergoing chemo-endocrine therapy by Xiaoyan Wu, Xunxi Lu, Wenchuan Zhang, Xiaorong Zhong, Hong Bu, Zhang Zhang

“…The model's performance was evaluated using AUC and decision curve analysis (DCA). …”
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Tumor cell-released autophagosomes (TRAPs) induce PD-L1-decorated NETs that suppress T-cell function to promote breast cancer pulmonary metastasis by Jing Chen, Ning Pan, Li-Xin Wang, Xiaohe Zhou, Chengdong Wu, Xuru Wang, Xiaotong Sun, Bohao Chen, Shiya Zheng, Yiting Wei, Yuyang Wu, Fengjiao Zhu, Jinpeng Chen, Huabiao Chen

“…Immunofluorescence, ELISA, Western blotting, RNA sequencing, and flow cytometry were employed to dissect the signaling pathways leading to NET production and to explore their immunosuppressive effects, particularly focusing on the impact of NETs on T-cell function. …”
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Rhaponticin inhibits the proliferation, migration, and invasion of head and neck squamous cell carcinoma (HNSCC) cells through modulation of the IL6/STAT3 signaling pathway by Hongcheng Wei, Shanshan Wang, Jiayue Wan, Sicheng Li, Wei Wang, Jiajun Zhu, Lin Jiang, Yisen Shao, Yuan Wu

“…Additionally, we analyzed single-cell RNA sequencing (scRNA-seq) data from the GEO database to compare IL6 expression levels in tumor and normal tissues and evaluated its prognostic impact using Receiver Operating Characteristic (ROC) curve analysis. …”
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Development and validation of a prognostic staging system for primary plasma cell leukemia by Mengru Tian, Gang An, Weijun Fu, Wenqiang Yan, Lu Li, Chunyan Sun, Zhenyu Li, Lijuan Chen, Aijun Liao, Guangxun Gao, Xiaoqi Qin, Mengyao Li, Chunrui Li, Hua Xue, Li Gao, Yi Wang, Aili He, Fan Zhou, Dongmei Guo, Yujun Dong, Zhihong Fang, Xiaoxia Chu, Jianqing Mi, Chengcheng Fu, Hui Zeng, Shuling Hou, Xiaotao Wang, Hua Wang, Yongqiang Wei, Xinyue Liang, Xingcheng Yi, Yue Sun, Lugui Qiu, Yun Dai, Fengyan Jin

“…Genome-wide DNA and RNA sequencing were performed to explore the molecular basis for inter-stage clinical heterogeneity. …”
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ADRB2 inhibition suppresses cancer immune evasion by regulating tumor SOX10-PD-L1 axis and T cell function by Yang Wang, Wei Zhang, Yu Zhang, Ling Chen, Shiyu Wang, Kun Song, Feng Yu, Jing Ouyang, Shan Cao, Cong Peng, Dongbo Liu, Yingying Dai, Wenwu Pei, Ziyang Hong, Weihua Huang, Yijing He, Panpan Liu, Hanying Yi, Gan Zhou, Howard McLeod

“…RNA sequencing analysis highlighted the importance of the transcription factor SRY-related HMG-box 10 (SOX10), which transcriptionally regulates programmed death-ligand 1 (PD-L1). …”
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A dietary combination of red yeast rice, phytosterol ester and lycopene ameliorates hypercholesterolemia by regulating gut microbiota and activating hepatic FXR-LDLR/ABCG pathway i... by Jingxian Xu, Jingxian Xu, Xin Huang, Xin Huang, Fei Pei, Yuzhu Chen, Yuzhu Chen, Cunzheng Zhang, Cunzheng Zhang, Lingling Zhao, Hua Zhang, Jindong Zhang, Jindong Zhang, Liping Duan, Liping Duan

“…The effects of these interventions on obesity, serum cholesterol, and glucose tolerance were evaluated. Mechanistic insights were gained through fecal 16S rRNA sequencing, targeted metabolomic profiling, and molecular analysis of liver and intestinal tissues using western blotting, qPCR, and immunofluorescence techniques.ResultsCompared to the HFHC group, low and high doses of the RPL combination reduced serum low-density lipoprotein cholesterol (LDL-C) levels by 33 and 20%, respectively, whereas simvastatin achieved a 22% reduction. …”
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The mechanism of Guanxin Qiwei dropping pills target Dubosiella to improve atherosclerosis by Yuanhong Liao, Yuanhong Liao, Jun Li, Jun Li, Yuxin Li, Jing Liu, Jing Liu, Tingting Chen, Jingkun Lu, Hongxia Li, Qian Zhang, Qian Zhang, Yuewu Wang, Yuewu Wang

“…Additionally, fecal samples were subjected to 16S rRNA sequencing to investigate the effects of GXQW on intestinal dysbacteriosis. …”
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Ligilactobacillus salivarius LZZAY01 accelerated autophagy and apoptosis in colon cancer cells and improved gut microbiota in CAC mice by Wenhong Yang, Tao Li, Shixiang An, Rong Chen, Yuxin Zhao, Jiaxian Cui, Mingyu Zhang, Jingkun Lu, Yunpeng Tian, Lili Bao, Pengwei Zhao

“…The impact of LZZAY01 on cancer cell lines CT-26, HCT-116, and SW-620 was evaluated by conducting cytotoxicity and clonogenicity tests. …”
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Collateral susceptibility-guided alternation of ceftolozane/tazobactam with imipenem prevents resistance development in XDR Pseudomonas aeruginosa biofilms by María Fernández-Billón, Elena Jordana-Lluch, Aina E. Llambías-Cabot, María A. Gomis-Font, Pablo Fraile-Ribot, Rosa I. Torrandell, Pamela J. Colman-Vega, Óscar Murillo, María D. Macià, Antonio Oliver

“…However, these mutations confer collateral susceptibility to carbapenems. Thus we aimed to evaluate the therapeutic efficacy and the prevention of resistance development of regimen alternating ceftolozane/tazobactam and imipenem. …”
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A novel vasculogenic mimicry-related nomogram predicts prognosis in hepatocellular carcinoma by Yun Zhong, Yun Zhong, Yun Zhong, Yun Zhong, Fadian Ding, Fadian Ding, Fadian Ding, Fadian Ding, Han Zhang, Han Zhang, Han Zhang, Han Zhang, Denghan Zhang, Denghan Zhang, Denghan Zhang, Denghan Zhang, Xiang Zhang, Xiang Zhang, Xiang Zhang, Xiang Zhang, Shangeng Weng, Shangeng Weng, Shangeng Weng, Shangeng Weng

“…In this study, we aim to create a VM-related, gene-based prediction model to evaluate the prognosis and immune infiltration in HCC patients.Materials and methodsA total of 364 patients from the TCGA database and 242 patients from the GEO database with complete clinical information and transcriptome sequencing data were enrolled in this study. …”
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The MFGE8/integrin β3 axis mitigates experimental neutrophilic asthma by suppressing NLRP3-Caspase-1 pathway-mediated NETosis by Shanshan Wang, Tang Kun, Zhen Tian, Mengqi Huang, Yan Fan, Boyu Li, Hongyan Zheng, Rongman Xu, Meijia Wang, Jianping Zhao, Jungang Xie

“…Further mechanistic studies, involving gene sequencing of sputum cells and lung tissue biopsies of asthmatic patients from GEO databases, identified neutrophil extracellular traps (NETs) as the critical factor in the progression of neutrophilic asthma. …”
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Construction of ceRNA networks of lncRNA and miRNA associated with intramuscular fat deposition in Ujumqin sheep by Mingxi Lan, Qing Qin, Yuchun Xie, Yuchun Xie, Chongyan Zhang, Chongyan Zhang, Chongyan Zhang, Zhichen Liu, Xiaolong Xu, Jingwen Zhang, Songsong Xu, Ji Yang, Haijun Zhang, Suhe Alatan, Zhixin Wang, Zhixin Wang, Zhixin Wang, Zhihong Liu, Zhihong Liu, Zhihong Liu

“…Exploring the role of non-coding RNAs, particularly lncRNAs and miRNAs, in IMF deposition can clarify its complex genetics and offer resources for breeding Inner Mongolian local breeds.MethodsWe evaluated carcass and lamb meat quality parameters using 60 six-month-old Ujumqin sheep with similar body weights. …”
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