Candida albicans-Derived β-Glucan as a Novel Modulator of Tumor Microenvironment: Targeting Macrophage Polarization and Inducing Ferroptosis in Lung Cancer by He X, Ran Q, Li X, Xiong A, Zhang L, Jiang M, Bai L, Peng D, Wang J, Sun B, Li G

“…This study investigates the inhibitory effects of β-glucan extracted from Candida albicans on lung cancer progression, focusing on its impact on TAM polarization and the induction of ferroptosis, a form of regulated cell death.Methods: Utilizing both in vivo animal models and in vitro cellular assays, we assessed the impact of β-glucan on tumor growth, cellular proliferation, and migration. We evaluated TAM polarization by analyzing the expression of M1 and M2 markers and identified differentially expressed genes (DEGs) related to ferroptosis. …”
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USC-Derived Small Extracellular Vesicles-Functionalized Scaffolds Promote Scarless Vaginal Defect Repair via Delivery of Decorin and DUSP3 Proteins by Xu Y, Li J, Qiu Y, Wu F, Xue Z, Liu B, Fan H, Zhou Y, Wu Q

“…Immunofluorescence staining, Western blot, qRT-PCR and collagen gel contraction analyses were conducted to evaluate the antifibrotic effects of USC-sEVs. RNA sequencing was employed to elucidate the underlying mechanisms involved. …”
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Characterizing the impact of podophyllotoxin on pulmonary toxicity and gut-lung microbiota interactions in SD rats based on TEC concept by Min Wang, Xiao Ma, Junjie He, Jiaxing Sun, Feng Cai, Chuanxin Liu, Jiajia Duan

“…In this study, we examined injury phenotypes in rats, evaluated pulmonary pathological changes, measured pro-inflammatory factors, and conducted comprehensive analyses of both pulmonary and gut microbiomes and metabolomics. …”
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Mining, validating, and quantifying circular RNA transcriptome from total RNA as a biomarker or target by Tan Ze Wang, Raj Kumar Thapa, Frank Yu, Ayesha Warsi, Kassie Rong, Pious Jose, Tahereh Kashkoulinejad-Kouhi, Phyo Wei Win, Christina A. Castellani, Tina Mele, Xiufen Zheng

“…PBMCs were isolated from sepsis patients before and after intensive care, and RNA sequencing was conducted with total RNA. RNA-seq data for circRNAs were analyzed using four pipelines. …”
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Detrimental Effects of Space Flight on the Lumbar Spine May Be Correlated to Baseline Degeneration: Insights From an Advanced MR Imaging Study by Bokhari R, Bisson DG, Fortin M, Vigouroux M, Cata JP, Hwang KP, Chen MM, Ceniza-Bordallo G, Ouellet JA, Ingelmo PM

“…The water content and glycosaminoglycan (GAGs) levels in the lumbar IVDs were evaluated using DIXON water-only phase imaging and T1rho MRI sequences. …”
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Coix Seed Oil Alleviates DSS-Induced Ulcerative Colitis via Intestinal Barrier Repair and Ferroptosis Regulation by Zeng YX, Li NR, Deng BY, Gu YF, Lu SF, Liu Y

“…Besides, the effect of CSO was further evaluated by observing the intercellular junctions of the colon tissues of each group under electron microscope. …”
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PM2.5 Induces Cell-Specific Transcriptomic Alterations in the Lungs of Juvenile Mice by Gui B, Li Y, Li K, Wang J, Fan W, Li L, Wu Q, Chen H

“…Lung histopathology was evaluated through hematoxylin and eosin staining.Results: Histological staining indicated that PM2.5 inhalation induces structural damage in the lung tissue. …”
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Development of ELISA test for the quality control of Pseudomonas aeruginosa recombinant vaccine based on the hybrid recombinant protein by A. V. Soldatenkova, A. M. Kudryashova, N. F. Gavrilova, I. V. Yakovleva, O. V. Borisova, V. V. Sviridov, N. A. Mikhailova

“…A hybrid recombinant protein containing the amino  acid sequences of the three  most significant Pseudomonas aeruginosa antigens  (membrane proteins OprF, OprI  and toxoid  aTox)  was incorporated into a vaccine against Pseudomonas infection. …”
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Multi-omics analysis reveals the efficacy of two probiotic strains in managing feline chronic kidney disease through gut microbiome and host metabolome by Hsiao-Wen Huang, Tzu-Chien Kuo, Tzu-Chien Kuo, Ya-Jane Lee, Ya-Jane Lee, Ming-Ju Chen, Ming-Ju Chen

“…In this pilot study, we aimed to evaluate the potential effects of an 8-week Lm intervention in cats with stage 2–3 CKD and to investigate the underlying host-microbiota interactions through integrated multi-omics analysis. …”
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A multi-module enhanced YOLOv8 framework for accurate AO classification of distal radius fractures: SCFAST-YOLO by Yu Wang, Haifu Sun, Tiankai Jiang, JunFeng Shi, JunFeng Shi, Qin Wang, Qin Wang, Hongwei Yang, Hongwei Yang, Yusen Qiao

“…Finally, we implement a Target-Aware Dual Detection Head that employs task decomposition to enhance localization precision.Results and discussionEvaluated on our FHSU-DRF dataset (332 cases, 1,456 CT sequences), SCFAST-YOLO achieves 91.8% mAP@0.5 and 87.2% classification accuracy for AO types, surpassing baseline YOLOv8 by 2.1 and 2.3 percentage points respectively. …”
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PARP3 promotes macrophage inflammation via mono ADP ribosylation of Ppia Glu140 by Runjie Fan, Rongxing Zhu, Xiangxiu Cao, Shuhui Ye, Fengyi Gao, Yue Wu, Wanxin Yao, Guang Liang, Yanmei Zhang

“…A mouse ALI model was used to evaluate the lung-protective and therapeutic effects of PARP3 inhibitor ME0328 by detecting inflammatory cytokines, phosphorylation of p65 and lung histopathology. …”
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Melanoma Vaccines: Comparing Novel Adjuvant Treatments in High-Risk Patients by Joseph C. Broderick, Alexandra M. Adams, Elizabeth L. Barbera, Spencer Van Decar, Guy T. Clifton, George E. Peoples

“…<b>Conclusions:</b> While both vaccines demonstrated promising efficacy, future phase 3 trials are needed to further evaluate their potential as adjuvant therapies for melanoma. …”
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Genetic landscape and ocular biometric correlations in microspherophakia: insights from a comprehensive patient cohort by Yan Liu, Yang Sun, Qiuyi Huo, Linghao Song, Xinyue Wang, Xin Shen, Ye Zhao, Tianhui Chen, Yongxiang Jiang

“…Methods Patients diagnosed with MSP at the Eye and ENT Hospital of Fudan University, Shanghai, were included in the study and all underwent panel-based next-generation sequencing and bioinformatics analysis. Comprehensive ophthalmologic evaluations were conducted for each participant. …”
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Dynamic transcriptome and metabolome analyses of two sweet corn lines under artificial aging treatment by Zili Zhang, Ruichun Yang, Lei Gao, Shilin Huang, Feng Jiang, Qingchun Chen, Pengfei Liu, Faqiang Feng

“…Results Using two inbred lines with significant differences in seed artificial aging tolerance, RNA sequencing and non-targeted metabolomic analysis were employed to extensively evaluate transcripts and metabolites in seeds that underwent artificial aging. …”
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Exploration of Epigenetic Mechanisms and Biomarkers Among Patients with Very-Late-Onset Schizophrenia-Like Psychosis by Gan Y, Yue W, Sun J, Yang D, Fang C, Zhou Z, Yin J, Zhou H

“…Machine learning algorithms generated diagnostic models, with classification performance evaluated using Area Under the Curve (AUC) metrics.Results: Analysis revealed distinct DNA methylation signatures in VLOSLP patients compared to controls. …”
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Potential of <i>LP</i> as a Biocontrol Agent for <i>Vibriosis</i> in Abalone Farming by Ling Ke, Chenyu Huang, Song Peng, Mengshi Zhao, Fengqiang Lin, Zhaolong Li

“…The resilience, antimicrobial activity, and in vivo antagonistic efficacy of the strains were evaluated through stress tolerance assays, agar diffusion tests, and animal experiments. …”
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Development and validation of machine learning-based diagnostic models using blood transcriptomics for early childhood diabetes prediction by Xin Huang, Xin Huang, Di Ouyang, Weiming Xie, Huawei Zhuang, Siyu Gao, Pan Liu, Lizhong Guo

“…This study aimed to develop and validate machine learning algorithms utilizing transcriptomic signatures to predict T1DM onset in children up to 46 months before clinical diagnosis.MethodsWe analyzed 247 peripheral blood RNA-sequencing samples from pre-diabetic children and age-matched healthy controls. …”
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Influence of light quality on the profiling of metabolites and the status of Lactobacillus community in the phyllosphere of hydroponically grown ginseng by Nooruddin Bin Sadiq, Ji-Eun Lee, Muhammad Hamayun, Seda Nur Kabadayı, Na-Yun Park, Bokyung Lee, Ho-Youn Kim

“…Detailed insights into the microbial community were obtained through 16S rRNA sequencing. A multivariate analysis was performed to distinguish the effects of different LED treatments of the phyllosphere microbiota. …”
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Clinical application of exempting anti-D monitoring in pregnant women with Asian-type DEL by YI Pin, OU Ziyu, SUN Xiaoxiao, WANG Mingming, WU Changlin, SHAO Chaopeng

“…Absorption and elution tests, DEL genotyping, and gene sequencing were used to divide the pregnant women into the Asian-type DEL group and the true negative group. …”
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Genetic Spectrum of Lithuanian Familial Hypercholesterolemia Patients by Urte Aliosaitiene, Rimante Cerkauskiene, Aleksandras Laucevicius, Migle Vilniskyte, Viktoras Sutkus, Antanas Mainelis, Birute Burnyte, Jurate Barysiene, Zaneta Petrulioniene

“…Children were analyzed separately. Next-generation sequencing (NGS) has been chosen as the most appropriate technique for genetic testing. …”
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