Effect of Sodium Butyrate and Sishen Pill Combination on Diarrhea with Kidney-Yang Deficiency Syndrome Was Associated with Intestinal Mucosal Microbiota and Immune Barrier by Di J, Guo M, Peng M, Fang L, Shen J, Xiao N, Tan Z

“…Kidney and intestinal histology were evaluated via hematoxylin‒eosin (HE) staining. The characteristics of the small intestinal mucosal microbiota under different interventions were analysed via 16S rRNA sequencing.Results: Compared with natural recovery, the combination of 100 mg/kg sodium butyrate + 50% SSP significantly restored colonic sIgA levels (p< 0.05) and MUC2 levels, indicating an improvement in intestinal mucosal barrier function. …”
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Determination of the effect of pyocyanin and Saccharomyces boulardii on gut microbiota and TLR4/MyD88/NF-κB and NLRP3 signaling pathways in sepsis induced by cecal ligation and pun... by Mahmut Ucar, Ozgur Celebi, Demet Celebi, Sumeyye Baser, Mustafa Can Guler, Ayhan Tanyeli, Metin Kılıclıoglu, Ahmet Yılmaz, Serkan Yıldırım

“…The experimental design assessed the synergistic or antagonistic effects of single and combined treatments using molecular, microbiome, and immunohistochemical parameters to evaluate histopathological damage and microbial ecological dynamics. …”
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Lipid-encapsulated gold nanoparticles: an advanced strategy for attenuating the inflammatory response in SARS-CoV-2 infection by Sanjeevram Dhandapani, Yujeong Ha, Rongbo Wang, Tae Woo Kwon, Ik-Hyun Cho, Yeon-Ju Kim

“…The KGS-NE exhibited a dose-dependent suppression of HCoV-OC43 viral replication in Vero E6 cells. RNA sequencing analysis unveiled differentially expressed genes (DEGs) specifically involved in the ABC transporters signaling pathway. …”
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MRI-based habitat imaging predicts high-risk molecular subtypes and early risk assessment of lower-grade gliomas by Xiangli Yang, Wenju Niu, Kai Wu, Guoqiang Yang, Hui Zhang

“…Identifying high-risk molecular subtypes early in LrGGs and conducting preoperative prognostic evaluations are crucial for precise clinical diagnosis and treatment. …”
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Enhancement of host defense against Helicobacter pylori infection through modulation of the gastrointestinal microenvironment by Lactiplantibacillus plantarum Lp05 by Yao Dong, Mei Han, Yongmei Qi, Ying Wu, Zhipeng Zhou, Dacheng Jiang, Zhonghui Gai

“…Microbial community structure in the stomach and intestines was evaluated through 16S rRNA gene sequencing.ResultsIn vitro studies showed Lp05 and H. pylori formed co-aggregates, with Lp05 potentially disrupting H. pylori cell structure, reducing its stomach colonization. …”
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Comprehensive prognostic gene identification and functional characterization of GRAMD1A in Wilms tumor: development of risk prediction models and therapeutic implications by Qiang Zeng, Junfeng Tao, Lilu Qin, Yong Zeng, Zhong Liu, Mingxian Xu, Linshan Zeng

“…This study aimed to identify key prognostic genes and pathways in WT, construct risk prediction models, and validate their role in tumor progression.MethodsRNA sequencing and clinical data from 136 WT patients were obtained from the TARGET database. …”
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Role of Magnetic Resonance Imaging in the Low Back Pain in Young Adults at a Tertiary Care Hospital in Northeast India by R. Harshith, Jaybrata Ray

“…Aims and Objectives: This study aims to evaluate degenerative changes in the lumbar spine of young adults aged 18–39 years with low back pain (LBP), using magnetic resonance imaging (MRI). …”
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Zinc sulfate improves insulin resistance, oxidative stress and apoptosis in liver tissues of PCOS rats through the NF-κB pathway by Yi-fan Kang, Jia-yi Zhao, Jian-rong Liu

“…Histological examination of ovarian and liver tissues was performed using hematoxylin and eosin (HE) staining, while hepatic lipid accumulation was evaluated by Oil Red O staining. Transmission electron microscopy was employed to examine liver cell ultrastructure, and TUNEL staining was used to assess hepatocellular apoptosis. …”
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Characterisation of macrophages in healthy and diseased livers in mice: identification of necrotic lesion-associated macrophages by Yang Wang, Dechun Feng, Bin Gao, Bryan Mackowiak, Yukun Guan, Luca Maccioni

“…Zone 1 KCs exhibited higher phagocytic activity than Zone 2/3 KCs and MoMFs. We simultaneously evaluated cell proliferation and apoptosis on one slide and found that proliferation and apoptosis of KCs and MoMFs significantly increased in acutely injured livers. …”
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Impact of Land Use Types on Soil Physico-Chemical Properties, Microbial Communities, and Their Fungistatic Effects by Giuseppina Iacomino, Mohamed Idbella, Salvatore Gaglione, Ahmed M. Abd-ElGawad, Giuliano Bonanomi

“…Soil characterization encompassed organic matter, pH, total nitrogen, C/N ratio, key cations (Ca²⁺, Mg²⁺, K⁺, Na⁺), enzymatic activities, microbial biomass, and soil microbiota analyzed through high-throughput sequencing of 16s rRNA genes. Fungistasis was evaluated against the pathogenic fungi <i>Botrytis cinerea</i> and the beneficial microbe <i>Trichoderma harzianum</i>. …”
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Heme Oxygenase-1 Modulates Macrophage Polarization Through Endothelial Exosomal miR-184-3p and Reduces Sepsis-Induce Lung Injury by Chen W, Zhang Y, Chen J, Dong S, Wu X, Wu Y, Du Z, Yang Y, Gong L, Yu J

“…Exosomes from PMVECs were isolated, and differential expression of microRNA (miRNA) was determined by sequencing. An in vitro co-culture system involving Murine Alveolar Macrophage Cell Line (MH-S cells) and HO-1/ PMVECs-derived exosomes (HP-exos) was used to investigate the underlying mechanisms. …”
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Association between taurine transporter SLC6A6 and breast cancer development and prognosis: a Mendelian randomization analysis by Xinping Huang, Hailong Shu, Man Xu, Weixiao Ke, Wanting Chen, Wenbin Huang, Hai Wang, Yifan Zhu, Yongxia Yang

“…Mendelian randomization (MR) analysis was used to evaluate the causal relationship between immune cell subsets and breast cancer risk. …”
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Effect of conventional grain-fed and grass-fed feeding systems on fecal microbiota and shiga toxin-producing Escherichia coli in beef cattle by Sudipta Talukder, Frederick Yang, Sarah Klopatek, James Oltjen, Xiang Yang

“…The microbial DNA was extracted and sequenced for 16 S rRNA gene for microbiota analysis. …”
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GPD1L as a potential biomarker associated with Treg cell infiltration and lipid metabolism in clear cell renal cell carcinoma by Ming Yang, Dejiang Pang, Chuhui Gong, Kangping Song, Hongbo Ma, Yu Yang, Shujin Guo, Liqiong Wang

“…The predictive value of GPD1L was evaluated by survival analysis, ROC curve and Cox regression analysis. …”
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SORBS3-β suppresses lymph node metastasis in cervical cancer by promoting the ubiquitination of β-catenin by Huating Sun, Yinghui Zhang, Fang Wang, Zizhao Wang, Yuhong Zhang, Youguo Chen, Li Wang, Jinhua Zhou

“…Functional studies, including siRNA-mediated knockdown of SORBS3-α, lentiviral-mediated overexpression and knockdown of SORBS3-β, Transwell migration, lymphangiogenesis assays, and in vivo footpad xenograft models, were conducted to evaluate the role of SORBS3 isoforms in LNM. SORBS3 DNA methylation mechanisms were analyzed by MSP and Targeted Bisulfite sequencing. …”
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Integrated Profiling Delineated KIF18A as a Significant Biomarker Associated with Both Prognostic Outcomes and Immune Response in Pancreatic Cancer by Nan K, Zhang L, Zou Y, Geng Z, Huang J, Peng Y, Yin S, Zhang M

“…However, its role in pancreatic adenocarcinoma (PAAD) remains unclear.Methods: To evaluate that role, RNA sequencing datasets, complemented by pertinent clinical metadata, were procured from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) repositories. …”
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Diversity, expression, and structural modeling of sugar transporters in Anisakis simplex s. s. L3 and L4 larvae: an in vitro and in silico study by Iwona Polak, Robert Stryiński, Łukasz Paukszto, Jan Paweł Jastrzębski, Iwona Bogacka, Elżbieta Łopieńska-Biernat

“…The FGTs were classified as members of the solute carrier family 2 (SLC2), while sweet-1 belonged to the SWEET transporter family. Full-length cDNA sequences were obtained, and encoded proteins structurally characterized using bioinformatic modeling. …”
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468 Effects of butyrate supplementation in modulation of gut microbiome and its metabolites in new-onset rheumatoid arthritis by Rebecca Blank, Alba Boix-Amoros, Erin R. Reilly, Kevin Bu, Ian Cunningham, Renuka R. Nayak, Andrew Patterson, Jose C. Clemente, Jose U. Scher

“…Fecal samples were collected at baseline and followed up for 16s rRNA sequencing and metabolite quantification by 1H NMR spectroscopy. …”
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Clinical phenotype and functional influence of GRIN2A variants in epilepsy‐aphasia syndrome by Lu Zhang, Yiran Duan, Rui Ma, Jiaqi Han, Na Pan, Lehong Gao, Yuping Wang

“…Methods Whole exome sequencing was conducted in enrolled patients with epilepsy‐aphasia syndromes, and GRIN2A variants were screened. …”
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Voxel- and surface-based morphometry in the cortical thickness and cortical and subcortical gray matter volume in patients with mild-to-moderate Alzheimer’s disease by Kaidi Li, Kaidi Li, Dingling Xie, Zhengyong Zhang, Chunyu Fu, Chunyu Fu, Chunyang Li

“…VBM and SBM analyses were performed on three-dimensional T1-weighted magnetization-prepared rapid gradient echo (3D T1-MPRAGE) imaging sequences to identify brain regions that exhibited statistically significant differences between the AD and HC groups. …”
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