New Associations with the HIV Predisposing and Protective Alleles of the Human Leukocyte Antigen System in a Peruvian Population by Daisy Obispo, Oscar Acosta, Maria L. Guevara, Susan Echavarría, Susan Espetia, María Dedios, Carlos Augusto Yabar, Ricardo Fujita

“…The accurate determination of an individual’s unique human leukocyte antigen (HLA) allele holds important significance in evaluating the risk associated with autoimmune and infectious diseases, such as human immunodeficiency virus (HIV) infection. …”
Get full text

Stratification system with dual human endogenous retroviruses for predicting immunotherapy efficacy in metastatic clear-cell renal cell carcinoma by Li Xu, Stephane Oudard, Catherine Sautes-Fridman, Xiaoping Su, Gabriel G. Malouf, Virginie Verkarre, Salma Kotti, Fangrong Yan, Yann-Alexandre Vano, Wolf Herve Fridman, Cheng-Ming Sun, Xiaofan Lu, Wenxuan Cheng

“…ERV expression was quantified using the ERVmap algorithm from RNA sequencing data. Our primary objective was to correlate ERV expression with progression-free survival, with overall survival and time-to-second-treatment survival as secondary endpoints. …”
Get full text

Effect of herbal medicine Bojungikgi-tang on gut microbiome and symptoms in anorexic patients with atopic dermatitis: a randomized controlled trial by Boram Lee, Mi Mi Ko, You Mee Ahn, Hyo-Ju Park, So Young Jung, Hyun-A Jung, Hesol Lee, Pyung-Wha Kim, Yujin Choi, Kyungsun Han, Sarah Shin, Jeeyoun Jung

“…Fecal samples were collected before and after treatment, and 16S rRNA sequencing was performed to analyze the gut microbiota.ResultsThe BJT group showed a significant decrease in anorexia VAS scores compared to the waiting list group at 8 weeks. …”
Get full text

LncRNA NEAT1 promotes epithelial–mesenchymal transition in nasal polyp cells via the miR-199-3p/PAK4 axis by Shuman Li, Yu Jiang, Yalan Zhang, Bowen Zheng, Chao Yuan, Yang Shen, Yi Zhao, Tao Lu, Yucheng Yang

“…EMT marker expression was analyzed via Western blotting and immunofluorescence. Transcriptome sequencing identified PAK4 as a downstream effector. …”
Get full text

Short-term use of ceftriaxone sodium leads to intestinal barrier disruption and ultrastructural changes of kidney in SD rats by Wenli Zou, Yueming Liu, Wei Zhang, Bo Lin, Wei Shen, Yiwen Li, Qiang He, Juan Jin

“…The aim of this study was to evaluate and visualize the impact of antibiotics on colonic mucus and the microbial community, and to assess whether intestinal dysbacteriosis is involved in the pathogenesis and progression of extraintestinal diseases in vivo.Materials and Methods Twenty-one SD rats were randomly assigned into three groups followed by different experimental treatments. …”
Get full text

Prognostic impact of dynamic changes of type I melanoma antigen gene proteins CT7 (MAGE-C1/CT7) transcripts in multiple myeloma by Xuelin Dou, Fengrong Wang, Huan Chen, Yao Chen, Lei Wen, Yang Liu, Guorui Ruan, Xiaosu Zhao, Xiaojun Huang, Robert Peter Gale, Jin Lu, Jin Lu

“…We first analyzed single-cell ribonucleic acid sequencing data from public databases to evaluate the expression of MAGE-C1/CT7 in MM patients and showed that MAGE-C1/CT7 is highly and specifically expressed in the MM cells. …”
Get full text

Targeting and cytotoxicity of chimeric antigen receptor T cells grafted with PD1 extramembrane domain by Ang Zhang, Shenyu Wang, Yao Sun, Yikun Zhang, Long Zhao, Yang Yang, Yijian Zhang, Lei Xu, Yangyang Lei, Jie Du, Hu Chen, Lian Duan, Mingyi He, Lintao Shi, Lei Liu, Quanjun Wang, Liangding Hu, Bin Zhang

“…Additionally, PE CAR showed ameliorative differentiation, exhaustion, and apoptosis phenotypes as assessed by flow cytometry, RNA-sequencing, and metabolic parameter analysis, after encountering CD19 + PDL1 + tumor cells. …”
Get full text

PD-1IR2 promotes tumor evasion via deregulating CD8+ T cell function by Xiaodong Wang, Shan Gao, Xiaofeng Zhu, Hongbo Hu, Fang Guo, Shuwen Cheng, Chang Huang, Haojing Zang, Tongfeng Liu, Liqiang Duan, Xujie Zhao, Xuetong Wang, Chang Zhang, Facai Yang, Yinmin Gu

“…However, it is not clear whether PDCD1 encoding PD-1 exists as an IR splicing isoform and what underlying function of such isoform plays in tumor evasion.Methods An AS isoform of human PDCD1, characterized by the second IR and named PD-1IR2, was identified by reverse transcription-PCR (RT-PCR) and Sanger sequencing. The expression profile of PD1IR2 was assessed by quantitative RT-PCR and flow cytometry, while its function was evaluated through immune cell proliferation, cytokine interleukin 2 secretion, and tumor cell killing assays. …”
Get full text

CircITGB6 promotes ovarian cancer cisplatin resistance by resetting tumor-associated macrophage polarization toward the M2 phenotype by Jingjing Zhao, Yue Li, Yan Tang, Jian-Chuan Xia, Yue Huang, Mian He, Weijing Zhang, Libing Song, Han Li, Fan Luo, Xingyu Jiang, Tong Xiang, Qiuzhong Pan, Liming Cai, Desheng Weng, Yuhu Dai, Fengze Sun, Chaopin Yang, Jieying Yang, Yulong Han, Yanna Zhang

“…The role of circITGB6 in inducing cisplatin (CDDP) resistance was evaluated by clone formation, immunofluorescence and annexin V assays in vitro, and by intraperitoneal tumor model in vivo. …”
Get full text

Mesenchymal stromal cells promote the formation of lung cancer organoids via Kindlin-2 by Zhilin Sui, Xianxian Wu, Jiaxin Wang, ShihJye Tan, Chao Zhao, Zhentao Yu, Chuanyue Wu, Xiaoxiao Wang, Ling Guo

“…RNA sequencing was used to analyze the differences in gene expression profiles between LCOs co-cultured with fibroblast and paracancerous organoids (POs). …”
Get full text

Uncovering pyrethroid resistance mechanisms in Anopheles punctulatus group mosquitoes: A novel insight from Keerom, Papua, Indonesia by Lepa Syahrani, Puji B. S. Asih, Ismail E. Rozi, Astari Dwiranti, Dendi H. Permana, Irdayanti Irdayanti, Anom Bowolaksono, Din Syafruddin

“…Insecticide susceptibility was evaluated using 0.75% permethrin bioassays. Molecular identification and detection of kdr mutations (L1014F/S) were performed using species-specific polymerase chain reaction and sequencing protocols. …”
Get full text

Drug Pair of Astragali Radix–Ligustri Lucidi Fructus Alleviates Acute Kidney Injury in Mice Induced by Ischemia–Reperfusion Through Inhibiting Ferroptosis by Xuanhe Liu, Dan Zhang, Yuting Xie, Mengdan Wang, Xiaochun Chen, Weijie Yu, Yuming Ma, Jia Zeng, Qixuan Long, Guangrui Huang, Jie Geng, Anlong Xu

“…Renal function (serum creatinine, Scr; blood urea nitrogen, BUN), inflammatory cytokines (TNF-α, IFN-γ, IL-6), ferroptosis markers (GPX4, MDA, GSH, tissue iron), and pathological damage were evaluated. Transcriptomic sequencing and electron microscopy analyzed gene pathways and mitochondrial structure. …”
Get full text

The co-occurrence of tet(X4) and tmexCD2-toprJ2 mediated tigecycline resistance in Raoultella ornithinolytica by Weishuai Zhai, Lu Liu, Jijun Kang, Mengjin Xiao, Yiqing Wang, Yao Wang, Yingbo Shen, Congming Wu, Jianzhong Shen, Yang Wang, Dejun Liu

“…Additionally, a conjugation assay was conducted to evaluate the transferability of these plasmids, followed by a 15-day stability test to assess the persistence of the two resistance determinants. …”
Get full text

Targeting p-FGFR1^Y654 Enhances CD8+ T Cells Infiltration and Overcomes Immunotherapy Resistance in Esophageal Squamous Cell Carcinoma by Regulating the CXCL8–CXCR2 Axis... by Hong Luo, Liwei Wang, Hui Gao, Daijun Zhou, Yu Qiu, Lijia Yang, Jing Li, Dan Du, Xiaoli Huang, Yu Zhao, Zhongchun Qi, Yue Zhang, Xuemei Huang, Lihan Sun, Tao Xu, Dong Li

“…Through RNA sequencing analysis, databases such as the Cancer Genome Atlas (TCGA) and Gene Set Cancer Analysis (GSCA), and the reconstruction of transgenic mice using the humanized immune system, we validated the correlation between the expression of p-FGFR1^Y654 and CD8+ T cell infiltration. …”
Get full text

Comparative in vitro activity of Delafloxacin and other antimicrobials against isolates from patients with acute bacterial skin, skin-structure infection and osteomyelitis by Ághata Cardoso da Silva Ribeiro, Fernanda Fernandes Santos, Tiago Barcelos Valiatti, Michael Henrique Lenzi, Ingrid Nayara Marcelino Santos, Raíssa Fidelis Baêta Neves, Ikechukwu Benjamin Moses, Jaqueline Pilon de Meneses, Renata Gebara de Grande Di Sessa, Mauro José Salles, Ana Cristina Gales

“…The screening for mecA was carried out by PCR, while mutations in the Quinolone Resistance Determining Regions (QRDR), specifically gyrA and parC, were analyzed using PCR followed by Sanger sequencing. Results showed that delafloxacin exhibited greater in vitro potency (at least 64-times) than the other tested fluoroquinolones (levofloxacin and ciprofloxacin) when evaluating Staphylococcus aureus (MIC50 ≤0.008 mg/L) and coagulase-negative Staphylococcus (MIC50 0.06 mg/L). …”
Get full text

A novel missense single-nucleotide polymorphism (c.149G>A) in the bovine leptin gene and its association with growth traits in Madura cattle by Kuswati Kuswati, Irida Novianti, Rizki Prafitri, Wike Andre Septian, Rafika Febriani Putri, Chairdin Dwi Nugraha, Ahmad Furqon

“…Materials and Methods: Forty-five Madura cows (aged 2–4 years) were phenotypically evaluated for body weight (BW), wither height (WH), body length (BL), chest girth (CG), hip height (HH), head length (HL), and head width (HW). …”
Get full text

Anti-TGF-β/PD-L1 bispecific antibody synergizes with radiotherapy to enhance antitumor immunity and mitigate radiation-induced pulmonary fibrosis by Yuze Wu, Yuheng Yan, Yarong Guo, Mengke Niu, Binghan Zhou, Jing Zhang, Pengfei Zhou, Qian Chu, Qi Mei, Ming Yi, Kongming Wu

“…To comprehensively characterize changes in the tumor microenvironment, we utilized a combination of methods, including flow cytometry, IHC staining, multiplex inmunofluorecence and RNA sequencing. Additionally, we evaluated the impact of YM101 on RT-induced pulmonary fibrosis. …”
Get full text

Multidrug-Resistant <i>Klebsiella pneumoniae</i> Strains in a Hospital: Phylogenetic Analysis to Investigate Local Epidemiology by Maria Vittoria Ristori, Fabio Scarpa, Daria Sanna, Marco Casu, Nicola Petrosillo, Umile Giuseppe Longo, De Florio Lucia, Silvia Spoto, Rosa Maria Chiantia, Alessandro Caserta, Raffaella Rosy Vescio, Flavio Davini, Lucrezia Bani, Elisabetta Riva, Massimo Ciccozzi, Silvia Angeletti

“…To identify potential subgroups within genetic clusters and evaluate genetic variability among sequences, principal coordinate analysis (PCoA) was performed. …”
Get full text

Feasible and Rapid Screening of <i>IDH1/2</i> and <i>FLT3-TKD2</i> Mutations by High-Resolution Melting for Patients with Acute Myeloid Leukemia by José Vicente Gil, Sandra de las Heras, Alberto Miralles, Claudia Sargas, Marta Llop, Rebeca Rodríguez-Veiga, Laura Torres-Miñana, Blanca Boluda, Isabel Cano-Ferri, Evelyn Acuña-Cruz, Irene Navarro, Pilar Lloret-Madrid, Pau Montesinos, Eva Barragán

“…The method was applied to a cohort of 1363 AML patients, and its performance, including turnaround time, was evaluated through comparison with next-generation sequencing (NGS) results. …”
Get full text

SHED-derived exosome-mimetics promotes rotator cuff tendon-bone healing via macrophage immunomodulation through NF-κB suppression and autophagy activation by Yanwei He, Junzhe Sheng, Fashun Liu, Fangqi Li, Shihao Lu, Wenbo Chen, Li Yang, Peng Zhou, Zan Chen, Shiyi Chen, Zhiwen Luo, Junming Sun

“…Rotator cuff tendon-bone healing is impeded by inflammation and inadequate regeneration. This study evaluates exosome-mimetics (EMs) derived from stem cells of human exfoliated deciduous teeth (SHEDs) as a therapeutic strategy to enhance repair.SHED-EMs were synthesized via serial extrusion and characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blot. …”
Get full text