Mild Zika Virus Infection in Mice Without Motor Impairments Induces Working Memory Deficits, Anxiety-like Behaviors, and Dysregulation of Immunity and Synaptic Vesicle Pathways by Jaime Alexander Chivatá-Ávila, Paola Rojas-Estevez, Alejandra M. Muñoz-Suarez, Esthefanny Caro-Morales, Aura Caterine Rengifo, Orlando Torres-Fernández, Jose Manuel Lozano, Diego A. Álvarez-Díaz

“…Murine models of ZIKV infection can be used to mimic part of the spectrum of motor and cognitive deficits observed in humans. These can be evaluated through behavioral tests, enabling comparison with gene expression profiles and aiding in the characterization of ZIKV-induced NDDs. …”
Get full text

Genomic characterization of Leishmania (V.) braziliensis associated with antimony therapeutic failure and variable in vitro tolerance to amphotericin B by Laura Natalia Gonzalez-Garcia, Aura María Rodríguez-Guzmán, Carolina M. Vargas-León, Samanda Aponte, Liliana A. Bonilla-Valbuena, J. Manuel Matiz-González, Ana M Clavijo-Vanegas, Germán Andrés Duarte-Olaya, Catherine Aguilar-Buitrago, Daniel Alfonso Urrea, Jorge Duitama, Maria C. Echeverry

“…The isolates were categorized based on their AmB in vitro susceptibility in the amastigote stage, the intracellular parasitic form found in the vertebrate host. The whole genome sequences of the isolates were analyzed and compared with the reference genomes of L. …”
Get full text

Marine-Derived Peptides from <i>Phaeodactylum tricornutum</i> as Potential SARS-CoV-2 Mpro Inhibitors: An <i>In Silico</i> Approach by David Mauricio Cañedo-Figueroa, Marco Antonio Valdez-Flores, Claudia Desireé Norzagaray-Valenzuela, Loranda Calderón-Zamora, Ángel Radamés Rábago-Monzón, Josué Camberos-Barraza, Alma Marlene Guadrón-Llanos, Alberto Kousuke De la Herrán-Arita, Verónica Judith Picos-Cárdenas, Alejandro Camacho-Zamora, Alejandra Romero-Utrilla, Carlos Daniel Cordero-Rivera, Rosa María del Ángel, Moisés León-Juárez, José Manuel Reyes-Ruiz, Carlos Noe Farfan-Morales, Luis Adrián De Jesús-González, Juan Fidel Osuna-Ramos

“…LigPlot+ was used to assess molecular interactions, while NetMHCpan 4.1 and AVPpred evaluated immunogenicity and antiviral potential, respectively. …”
Get full text

AdipoRon ameliorates the progression of heart failure with preserved ejection fraction via mitigating lipid accumulation and fibrosis by Wuping Tan, Yijun Wang, Siyi Cheng, Zhihao Liu, Mengjie Xie, Lingpeng Song, Qinfang Qiu, Xiaofei Wang, Zeyan Li, Tianyuan Liu, Fuding Guo, Jun Wang, Xiaoya Zhou

“…Cardiac function was evaluated using echocardiography, and Postmortem analysis included RNA-sequencing, untargeted metabolomics, transmission electron microscopy and molecular biology methods. …”
Get full text

Plasma biomarkers of dairy intake and gut microbiome, and their association with incident carotid plaque: a prospective cohort study by Congmei Xiao, Qian Chen, Menglei Shuai, Chang Gao, Wanglong Gou, Zelei Miao, Ke Zhang, Luqi Shen, Xinxiu Liang, Chun Wang, Haili Zhong, Meng Ye, Fengzhe Xu, Jun Tang, Zengliang Jiang, Yue Xi, Yuanqing Fu, Yu-ming Chen, Ju-Sheng Zheng

“…Westlake Precision Birth Cohort was used to evaluate the potential associations between OCFA and the identified gut microbiota features in a younger population. …”
Get full text

Clinical features of HAdV-55 in children with respiratory tract infections: a retrospective case series and literature review by Lifen Rao, Yueqiang Fu, Ying Lu, Jianhua Wei, Zhongying Yang, Mengling Qi, Chengjun Liu, Yushun Wan, Enmei Liu, Na Zang

“…Clinical features were evaluated in conjunction with literature on HAdV-55 infections in children. …”
Get full text

Sodium Glucose Transporter 2 Inhibitor Protects Against Heart Failure With Preserved Ejection Fraction: Preclinical “2‐Hit” Model Reveals Autophagy Enhancement Via AMP‐Activated Pr... by Xinyu Hu, Dan Li, Weijie Chen, Hongyu Kuang, Dan Yang, Zhiyan Gong, Yuxiang Long, Guangliang Liu, Kai Wang, Mengshi Xia, Yanping Xu, Yuehui Yin

“…Mechanistic analysis, including RNA sequencing, Western blotting, and immunohistochemistry, was conducted. …”
Get full text

Jing-Yin-Gu-Biao formula protects mice from postinfluenza Staphylococcus aureus infection by ameliorating acute lung injury and improving hypercoagulable state via inhibiting NETos... by Biao Lei, Biao Lei, Jingwen Mu, Jingwen Mu, Guihua Xu, Xiaodong Yang, Xiaodong Yang, Wenbo Huang, Liang Hu, Dan Liu, Dan Liu, Ting Cheng, Ting Cheng, Yuhe Ma, Yuhe Ma, Lirong Xu, Lirong Xu, Qiankun Liang, Qiankun Liang, Yuan Lin, Linqiong Zhou, Linqiong Zhou, Linqiong Zhou, Chunxian Zhou, Chunxian Zhou, Wei Zhang, Wei Zhang, Wei Zhang, Yuejuan Zheng, Yuejuan Zheng

“…Single cell RNA-sequencing (scRNA-seq) data indicated that neutrophils had the highest cytokine score in lungs and JYGBF inhibited neutrophil chemotaxis, reactive oxygen species (ROS) biosynthesis and ERK1/2 cascades in neutrophils. …”
Get full text

Revealing the impact of Pseudomonas aeruginosa quorum sensing molecule 2’-aminoacetophenone on the human bronchial-airway epithelium and pulmonary endothelium using a human airway-... by Shifu Aggarwal, Shifu Aggarwal, Arijit Chakraborty, Arijit Chakraborty, Arijit Chakraborty, Vijay K. Singh, Stephen Lory, Katia Karalis, Laurence G. Rahme, Laurence G. Rahme, Laurence G. Rahme

“…Cells exposed to 2-AA (20 μm) through continuous flow for 12 hours were used for whole-genome RNA sequencing and analyzed for their responses and potential cross-talk. …”
Get full text

Investigation into the impact of chloride ion uptake inhibitors on tobacco plants by Hai Ding, Shengli Wang, Yan Zhang, Jianfei Dong, Ruonan Zhang, Bao Zhang, Shangyi Ma, Dong Liu, Ruibin Cui, Bin Chen, Qun Wang, Jian Sun, Shuo Xing, Zhaobao Wang, Minchong Shen, Weitao Wang, Huaibao Zhang

“…Specifically, the chloride ion content in upper leaves decreased from 1.33% to 0.83% under T1 treatment, while the T2 experimental group showed a reduction to 0.99%. Additionally, we evaluated the agronomic traits of tobacco plants and found that the application of chloride ion inhibitors promoted plant growth. …”
Get full text

Elevation of ISG15 promotes diabetic kidney disease by modulating renal tubular epithelial cell pyroptosis by Lingzhi Huang, Xinyi Chen, Yawen Shao, Shujun Deng, Chen Wang, Jianqiao Chen, Yongsheng Xie, Siming Yuan, Liqin Tang

“…Furthermore, RNA sequencing (RNA‐seq) and molecular biology techniques were employed to explore the signalling pathways potentially regulated by ISG15. …”
Get full text

Advancing offline magnetic resonance-guided prostate radiotherapy through dedicated imaging and deep learning-based automatic contouring of targets and neurovascular structures by Philipp Schubert, Matthias May, Daniel Höfler, Hans-Peter Fautz, Jana Hutter, Ricarda Merten, Sina Mansoorian, Thomas Weissmann, Lisa Deloch, Miriam Schonath, Nathalia Belmas, Felix Grabenbauer, Benjamin Frey, Udo Gaipl, Bernd-Niklas Axer, Juliane Szkitsak, Michael Uder, Christoph Bert, Rainer Fietkau, Florian Putz

“…Material and Methods: High-resolution 3D T2-weighted SPACE MRI sequences (<1 mm3 resolution) were obtained for 50 patients in treatment position. …”
Get full text

Curcumin supplementation accelerates high-altitude acclimatization, prevents polycythemia and modulates gut microbiota in male Han population: a randomized controlled trial by Jilei Hu, Hongmei Lang, Die Fan, Ting Wen, Jiaojiao Shi, Chunxiu Xiao, Yunming Li, Chao Kang, Peijie Shi, Peijie Shi, Lirong Shen, Ning Lin, Ning Lin, Ning Lin

“…The score of acute mountain sickness (AMS) was evaluated in the 2nd week after entering the plateau (T2) and T3. …”
Get full text

Combining single-cell analysis and molecular docking techniques to construct a prognostic model for colon adenocarcinoma and uncovering inhibin subunit βb as a novel therapeutic ta... by Qinqing Wu, Qinqing Wu, Lu Ye, Yuwei Wu, Mengyu Zhao, Mengyu Zhao, Jiaxin Lu, Yanping Yu, Yanping Yu, Yixiao Niu, Yixiao Niu, Luxiao Zhang, Peijun Zuo, Peijun Zuo

“…We also investigated the roles and interactions of these genes in the tumor microenvironment by analyzing single-cell RNA sequencing data. Additionally, we employed molecular docking techniques to evaluate the potential of inhibin subunit beta B (INHBB) as therapeutic targets and to assess the binding affinity of candidate drugs. …”
Get full text

Phosphatidylethanolamine promotes macrophage senescence and liver injury by activating endoplasmic reticulum stress by HAN Longchuan, LI Yue, ZOU Zhihui, LUO Jing, LI Ruoyi, ZHANG Yingting, TANG Xinxin, TIAN Lihong, LU Yuheng, HUANG Ying, HE Ming, FU Yinkun

“…Senescence markers and SASP factors, including senescence-associated β-galactosidase (SA-β-gal), cell cycle inhibitor p21, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), were evaluated using SA-β-gal staining, quantitative real-time PCR, and Western blotting. …”
Get full text

Cannabidiol-Loaded Retinal Organoid-Derived Extracellular Vesicles Protect Oxidatively Stressed ARPE-19 Cells by Peggy Arthur, Sangeetha Kandoi, Anil Kalvala, Breana Boirie, Aakash Nathani, Mounika Aare, Santanu Bhattacharya, Tanmay Kulkarni, Li Sun, Deepak A. Lamba, Yan Li, Mandip Singh

“…The goal of the current study is to establish and evaluate the encapsulation of a hydrophobic compound, cannabidiol (CBD), into retinal organoid-derived extracellular vesicles (EVs) for potential therapeutic use in AMD. …”
Get full text

Comparative Genomics of Rhamnolipid Synthesis and Monoaromatic Hydrocarbon Tolerance Genes in Environmental Pseudomonas aeruginosa strains [version 2; peer review: 2 approved] by Sandro B. Martel-Torres, Camila Castillo-Vilcahuaman, ROGER A. PALOMINO HUARCAYA, Susana M. Gutiérrez Moreno, Fernando A. Merino Rafael

“…DNA extraction, sequencing, and quality-controlled assembly, functional genome annotation was performed using BAKTA. …”
Get full text

MDM2 amplification in a real-world cohort of patients with biliary tract cancer from the Spanish RETUD gastrointestinal registry by A.J. Muñoz Martín, F. Castet, J. Soto Alsar, J. Adeva, P. Peinado, B. Graña, I. Alés Díaz, R.M. Rodríguez-Alonso, M. Lobo de Mena, R. Vera, I. Ruiz de Mena, S. Aguilar, S. Vega, L. Ortega Morán, T. Macarulla

“…Materials and methods: A real-world cohort of patients diagnosed with BTC (1 January 2017 to 31 December 2022) was evaluated (RETUD NCT06711211). Next-generation sequencing (NGS) testing was carried out. …”
Get full text

Metasilicate-based alkaline mineral water improves the growth performance of weaned piglets by maintaining gut-liver axis homeostasis through microbiota-mediated secondary bile aci... by Jian Chen, Kanwar K. Malhi, Xiaowei Li, Xiangwen Xu, Jianxun Kang, Bichen Zhao, Yaru Xu, Xuenan Li, Jinlong Li

“…To analyze the composition of the gut microbiota, 16S rRNA PacBio SMRT Gene Full-Length Sequencing was performed. Western blotting and immunofluorescence were employed to assess protein expression levels. …”
Get full text

Phase I study of ribociclib (CDK4/6 inhibitor) with spartalizumab (PD-1 inhibitor) with and without fulvestrant in metastatic hormone receptor-positive breast cancer or advanced ov... by Sara M Tolaney, Tianyu Li, Elizabeth A Mittendorf, Geoffrey I Shapiro, Stephanie K Dougan, Nabihah Tayob, Joyce F Liu, Khanh Do, Heather Parsons, Molly DiLullo, Eileen Wrabel, Lestat R Ali, Ana C Garrido-Castro, Noah Graham, Christina Herold, Jennifer Desrosiers, Shom Goel, Amy J Williams

“…The combination was also evaluated with fulvestrant in MBC.Methods In Cohort A, ribociclib was administered on Days 1–21 (28-day cycle) starting at 400 mg, and spartalizumab at 400 mg on Day 1. …”
Get full text