Effects and mechanisms of acupuncture for PIGD-subtype Parkinson’s disease via integration of fMRI and gut microbiota-metabolomics analysis: protocol for a prospective randomized c... by Jili Sheng, Jili Sheng, Yingqi Xu, Tao Liu, Jianfang Zhu, Caiyun Mu, Lihao Zhai, Shanhu Xu, Hanyi Wang, Xiangzhi Liu, Tao Liu, Xiaoqing Jin

“…Additionally, serum and stool samples will be collected for 16S ribosomal RNA sequencing, and liquid chromatography coupled with tandem mass spectrometry analysis (LC–MS/MS) will be employed to elucidate theunderlying mechanisms. …”
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Dietary supplementation with Epimedium contributes to the improvement of hormone levels, gut microbiota, and serum metabolite composition in the Chinese forest musk deer (Moschus b... by Shan Xie, Shan Xie, Qinlin Yang, Zaixiang Ying, Zaixiang Ying, Mingcheng Cai, Wenqiao Fan, Hanyu Gao, Hanyu Gao, Xiaolan Feng, Yongjiang Wu

“…This study aimed to evaluate the effects of dietary supplementation with Epimedium on the hormone levels, gut microbiota composition, and serum metabolism of the Chinese forest musk deer. …”
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Impact of Lysine to Methionine Ratios on Antioxidant Capacity and Immune Function in the Rumen of Tibetan Sheep: An RNA‐Seq Analysis by Fengshuo Zhang, Quyangangmao Su, Zhanhong Gao, Zhenling Wu, Qiurong Ji, Tingli He, Kaina Zhu, Xuan Chen, Yu Zhang, Shengzhen Hou, Linsheng Gui

“…Ninety male Tibetan sheep, weaned at 2 months of age, were randomly divided into three groups (1:1, 2:1 and 3:1 lysine ratios) and subjected to a 100‐day feeding trial. RNA sequencing (RNA‐seq) was utilized to analyse the impact of different AA ratios on gene expression in rumen tissue, whereas the levels of antioxidant enzymes (total antioxidant capacity [T‐AOC], superoxide dismutase [SOD], glutathione peroxidase [GSH‐Px] and catalase [CAT]) and immunoglobulins (immunoglobulin A [IgA], immunoglobulin G [IgG] and immunoglobulin M [IgM]) were evaluated. …”
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Chronic arsenic exposure-provoked biotoxicity involved in liver-microbiota-gut axis disruption in chickens based on multi-omics technologies by Jiayi Li, Changming Guo, Yan Liu, Biqi Han, Zhanjun Lv, Huijie Jiang, Siyu Li, Zhigang Zhang

“…Conclusion: Our study demonstrates that chronic inorganic arsenic exposure evokes liver-microbiota-gut axis disruption in chickens and establishes a scientific basis for evaluating health risk induced by environmental pollutant arsenic.…”
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Inverted day-night feeding during pregnancy affects the brain health of both maternal and fetal brains through increasing inflammation levels associated with dysbiosis of the gut m... by Xinyue Wang, Xiangju Kong, Yibo Ding, Mengqing An, Xuan Zhu, Yue Guan, Yucun Niu

“…Anxiety-like behavior was evaluated using the open-field test. Maternal caecal microbiota was analyzed using 16S rRNA sequencing. …”
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Inhibition of miR-615-3p enhances dentinogenesis in scaps via PVT1-mediated mitochondrial regulation by Haoqing Yang, Yishu Huang, Jiaxin Song, Xiao Han, Fengning Yuan, Zhipeng Fan

“…Mitochondrial function was evaluated by measuring reactive oxygen species (ROS) levels, membrane potential, and respiratory activity. …”
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The effects of a semen cuscutae flavonoids-based antidepressant treatment on microbiome and metabolome in mice by Qianfeng Shao, Qianfeng Shao, Sheng Zhou, Yue Li, Lin Jin, Xiaowei Fu, Tong Liu, Tong Liu, Jing Wang, Jing Wang, Shaohui Du, Shaohui Du, Che Chen

“…A range of standardized behavioral assays and physiological indicators were employed to evaluate the antidepressant effects of SCFs. Upon the confirmation of the effectiveness of the SCFs treatment, the composition, richness, and diversity of the fecal microbiota were assessed using 16S rRNA gene sequencing. …”
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FSTL1 accelerates nucleus pulposus-derived mesenchymal stem cell apoptosis in intervertebral disc degeneration by activating TGF-β-mediated Smad2/3 phosphorylation by Xu Yan, Jing-Yu Ding, Ren-Jie Zhang, Yan-Xin Wang, Lu-Ping Zhou, Hua-Qing Zhang, Liang Kang, Chong-Yu Jia, Xiao-Ying Liu, Cai-Liang Shen

“…Western blotting, qPCR, and immunofluorescence were used to evaluate follistatin-like 1 (FSTL1) expression in NP-MSCs. …”
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The impact of Sarcocystis infection on lamb flavor metabolites and its underlying molecular mechanisms by Kang Zhang, Huan Teng, Huan Teng, Li Zhu, Bin Ni, Lai Jiewei, Li Shanshan, Zhao Yunong, Xiao Guo, DanQu Lamu

“…IntroductionMeat flavor is a critical factor for consumers to evaluate meat quality and a key determinant of its market value. …”
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IL-6-induced long noncoding RNA MIR3142HG promotes tumorigenesis by interacting with thioredoxin-1 and STAT3 in human colorectal cancer by Daoquan Fang, Qian Feng, Baojian Zhou, Yangyang Liu, Yichu Lian, Yihui Zhang, Dichen Yang, Xintong Liu, Xiaomeng Shi, Wuhua Ni, Lei Jiang

“…Bioinformatics analysis and tissue microarray were utilized for evaluating the clinical value of MIR3142HG in CRC. Results We identified a lncRNA, MIR3142HG, regulated by Trx-1 knockdown and IL-6 treatment. …”
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A new yeast strain for valorisation of vinasse, a rum distillery waste product by Brigita Simonaviciene, Ayokunle Araoyinbo, Juwayria Ali, Jamie McGowan, David A. Fitzpatrick, Gary Jones, Celia Ferreira, Andrew R. Pitt, Corinne M. Spickett, Vincent Postis, Carine de Marcos Lousa

“…Whole genome sequencing (WGS) identified V1 as Pichia kudriavzevii. …”
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Dickkopf 1 impairs the tumor response to PD-1 blockade by inactivating CD8+ T cells in deficient mismatch repair colorectal cancer by Yuan Li, Jian Zheng, Zhizhong Pan, Penghui Zhou, Guochen Liu, Wu Jiang, Pei-Rong Ding, Zexian Liu, Qiaoqi Sui, Dingxin Liu, Jinghua Tang, Lingheng Kong, Kai Han, Leen Liao, Qingjian Ou, Binyi Xiao, Yihong Ling, Jiewei Chen, Zhixiang Zuo

“…However, whether DKK1 influences the tumor response to programmed cell death protein 1 (PD-1) blockade in colorectal cancers (CRCs) with deficient mismatch repair (dMMR) or microsatellite instability (MSI) has never been clarified.Methods Tumor tissues from 80 patients with dMMR CRC were evaluated for DKK1 expression and immune status via immunohistochemistry. …”
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Multimodal data integration for biologically-relevant artificial intelligence to guide adjuvant chemotherapy in stage II colorectal cancerResearch in context by Chenyi Xie, Ziyu Ning, Ting Guo, Lisha Yao, Xiaobo Chen, Wanghong Huang, Suyun Li, Jiahui Chen, Ke Zhao, Xiuwu Bian, Zhenhui Li, Yanqi Huang, Changhong Liang, Qingling Zhang, Zaiyi Liu

“…Histopathological patterns were evaluated to bridge the gap between the imaging and genetic information. …”
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INBRX-106: a hexavalent OX40 agonist that drives superior antitumor responses via optimized receptor clustering by William L Redmond, Yoshinobu Koguchi, Melissa J Kasiewicz, Annah S Rolig, Nisha Holay, Bryan Becklund, Anya Polovina, Sae Jeong Ahn, Brendan P Eckelman, Rashi Yadav, Thi Staebler, Noah D Simons, Yaiza Diaz de Durana

“…To address this, we developed INBRX-106, a hexavalent OX40 agonist designed to enhance receptor clustering independently of Fc-mediated crosslinking and boost antitumor T-cell responses.Methods We assessed INBRX-106’s effects on receptor clustering, signal transduction, and T-cell activation using NF-kß reporter assays, confocal microscopy, flow cytometry, and single-cell RNA sequencing. Therapeutic efficacy was evaluated in murine tumor models and ex vivo human samples. …”
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Diversity and functional roles of endophytic and rhizospheric microorganisms in Ophioglossum vulgatum L.: implications for bioactive compound synthesis by Xian-Nv Long, Xian-Nv Long, Xian-Nv Long, Xing-Kai Zhang, Yue Wu, Shu-Shuang Tang, Tai-Xiong Zheng, Di Chen, Guan-Hua Cao, Guan-Hua Cao, Guan-Hua Cao, Xu-Hong Zhou, Sen He, Sen He, Sen He

“…This study systematically characterized the endophytic and rhizospheric community structure, taxonomic diversity, and symbiotic networks within distinct compartments of O. vulgatum, while evaluating their potential associations with the accumulation of pharmacologically active metabolites.MethodsEndophytic and rhizospheric community profiling was conducted via Illumina sequencing, while bioactive compounds were identified using UPLC-ESI-MS/MS.ResultsRoots and leaves harbored beneficial bacteria (e.g., Methylobacterium, Streptomyces, Sphingomonas, and Flavobacterium). …”
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Longitudinal multimodal MRI analysis of lecanemab treatment in mild cognitive impairment: a pilot study of structural, perfusion, and microstructural changes by Toshiya Takahashi, Dinh Ha Duy Thuy, Shingo Takenaka, Sayaka Ono, Maya Fukui, Yasushi Okada, Tomohiko Asada, Kan Niimi, Kaku Kimura, Akio Ikeda, Ryosuke Takahashi, Riki Matsumoto, Hidenao Fukuyama

“…However, its effects on brain structure, cerebral perfusion, gray matter microstructure and white matter microstructure remain unclear.MethodsThis exploratory longitudinal study aimed to evaluate changes in brain volume, cerebral blood flow (CBF), and diffusion tensor imaging (DTI) measures over a 12-month treatment period in 8 patients with MCI receiving biweekly lecanemab infusions. …”
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Improved CAR internalization and recycling through transmembrane domain optimization reduces CAR-T cytokine release and exhaustion by Shufeng Xie, Shufeng Xie, Jinlan Long, Ruiheng Wang, Ruiheng Wang, Rufang Xiang, Rufang Xiang, Huajian Xian, Yixin Wang, Weiyu Dou, Wenjie Zhang, Dan Li, Ting Kang, Zhihong Chen, Zhihong Chen, Chunjun Zhao, Zhenshu Xu, Han Liu

“…Through co-culture with tumor cells, we evaluate CAR dynamic change, activation levels, exhaustion markers, mitochondrial function, and differentiation in both CAR-T cells. …”
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Assessing the impact of moxibustion on colonic mucosal integrity and gut microbiota in a rat model of cerebral ischemic stroke: insights from the “brain-gut axis” theory by Yi-Xia Ding, Yi-Xia Ding, Yi-Xia Ding, Liang-Liang Chen, Liang-Liang Chen, Liang-Liang Chen, Kui-Wu Li, Ling Zou, Lu-Min Liao, Xiao-Yu Han, Jie OuYang, Yue-Ping Wu, Wen-Dong Zhang, Wen-Dong Zhang, Wen-Dong Zhang, Hao Ran Chu, Hao Ran Chu, Hao Ran Chu

“…Gut microbiota composition was analyzed using 16S rDNA amplification sequencing. Intestinal mucosal permeability was evaluated using the FITC-Dextran tracer method. …”
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Influence of lactulose as a composition of organic-mineral feed additive on broiler chicken productivity, feed digestibility, and microbiome by Еlena A. Sizova, Daniil E. Shoshin, Еlena V. Yausheva, Anastasia P. Ivanishcheva, Ksenia S. Nechitailo, Kristina V. Ryazantseva

“…Cecal microbiota profiling was conducted using 16S ribosomal RNA gene sequencing on the MiSeq platform. Statistical analyses were performed using the Mann-Whitney U-test. …”
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Probiotic Characterization of <i>Lactiplantibacillus paraplantarum</i> SDN1.2 and Its Anti-Inflammatory Effect on <i>Klebsiella pneumoniae</i>-Infected Mammary Glands by Jia Cheng, Jingdi Tong, Can Li, Ziyan Wang, Hao Li, Meiyi Ren, Jinshang Song, Deyuan Song, Qinna Xie, Mingchao Liu

“…., beta-lactamases and tetracycline resistance genes), as supported by whole-genome sequencing, and significantly inhibits the growth of <i>K. pneumoniae</i>, as evaluated by antimicrobial tests. …”
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