A comparative pharmacological study of three Chinese traditional medicines found Blautia to be the key functional bacterium of Coptis chinensis Franch. and Phellodendri chinensis C... by Jiaguo Zhan, Jiale Cheng, Yanan Yang, Xiaofan Xu, Zhiyi Lu, Leilei Li, Haoyu Li, Qiannan Yang, Yanran Hu, Yuxin Song, Qingmiao Fan, Enwei Yang, Qianyue Liang, Shuangyong Sun, Shuangyong Sun, Feng Qiu, Feng Qiu, Ying Cao, Chongming Wu, Chongming Wu

“…Full-length 16S rRNA amplicon sequencing was utilized to assess changes in gut microbiota following drug interventions. …”
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Effect of fiber-modified kombucha tea on gut microbiota in healthy population: A randomized controlled trial (RCT) by Beatriz Arce-López, Guadalupe X. Bazan, Susana Molina, Maria Carmen Crespo, María García-Beccaria, Silvia Cruz-Gil, Cristina M. Fernández-Díaz, Ana Ramírez de Molina, Ricardo Ramos-Ruiz, Maria Isabel Espinosa-Salinas

“…Objective: This study aimed to evaluate the effects of fiber-enriched kombucha tea on biochemical parameters and gut microbiota composition in a healthy population. …”
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Late-stage diversification strategy for the synthesis of peptide acids and amides using hydrazides by Shoko Tanaka, Mizuki Kanno, Yosuke Tashiro, Tetsuo Narumi, Nobuyuki Mase, Kohei Sato

“…Azidation and subsequent treatment with BME afforded peptide acids an acceptable yield even in sequences containing amino acids that are prone to oxidation. …”
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SLC7A11 as a therapeutic target to attenuate phthalates-driven testosterone level decline in mice by Yi Zhao, Xue-Qi Wang, Rui-Qi Liu, Fu-Wei Jiang, Jia-Xin Wang, Ming-Shan Chen, Hao Zhang, Jia-Gen Cui, Yuan-Hang Chang, Jin-Long Li

“…Methods: We conducted validated experiments in vivo male mice model and in vitro mouse Leydig TM3 cell line, followed by RNA sequencing and metabolomic analysis. We evaluated the levels of testosterone synthesis-associated enzymes and ferroptosis-related indicators by using qRT-PCR and Western blotting. …”
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Detection of hypervirulent Klebsiella pneumoniae (hvKp) strains directly from spiked blood cultures using a commercial Loop-Mediated isothermal amplification (LAMP) assay by Domingo Fernández Vecilla, Jorge Rodríguez Grande, Nuria Fraile Valcárcel, Zaira Moure García, Sergio García Fernández, María Siller Ruiz, María Pía Roiz Mesones, José Luis Díaz de Tuesta del Arco, Mikel Joseba Urrutikoetxea Gutiérrez, María Catalina Lomoro, María Carmen Fariñas, Alain Ocampo-Sosa

“…Capsular serotyping and virulence gene detection were performed using PCR and whole-genome sequencing (WGS). The Eazyplex® hvKp LAMP assay was tested on spiked blood cultures inoculated with hvKp isolates. …”
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Bacterial communities and soil functionality in artificially remediated vegetation of the three gorges reservoir zone by Farkhanada Naz, Farkhanada Naz, Muhammad Arif, Tan Xue, Yangyi Chen, Shahid Ullah Khan, Li Changxiao, Li Changxiao

“…Our analysis of 360 soil samples led to the generation of high-quality sequences that provided insights into microbial diversity. …”
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Clinical activity of pembrolizumab in metastatic prostate cancer with microsatellite instability high (MSI-H) detected by circulating tumor DNA by Neeraj Agarwal, Oliver Sartor, Brian Lewis, Hani Babiker, Elisa Ledet, Lesli Kiedrowski, Pedro Barata, Roberto Nussenzveig, Benjamin Gerendash, Ellen Jaeger, Whitley Hatton, Jodi Layton, Alan Bryce, Cy Stein, Philip Saylor

“…To report a multi-institutional case series of patients with advanced microsatellite instability high (MSI-H) prostate adenocarcinoma identified with clinical cell-free DNA (cfDNA) next-generation sequencing (NGS) testing and treated with immune checkpoint inhibitors. …”
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Detection of β-lactam resistance genes in Gram-negative bacteria from positive blood cultures using a microchip-based molecular assay by Vittorio Ivagnes, Flavio De Maio, Ilaria Baccani, Alberto Antonelli, Giulia Menchinelli, Roberto Rosato, Giordana Cafaro, Giulia Santarelli, Federico Falletta, Tiziana D’Inzeo, Tiziana D’Inzeo, Maurizio Sanguinetti, Maurizio Sanguinetti, Teresa Spanu, Giulia De Angelis, Giulia De Angelis, Gian Maria Rossolini, Gian Maria Rossolini, Brunella Posteraro, Brunella Posteraro

“…Whole-genome sequencing (WGS) served as the reference assay for simulated samples and, selectively, for clinical samples. …”
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ABL1‒YAP1 axis in intestinal stem cell activated by deoxycholic acid contributes to hepatic steatosis by Tiancheng Mao, Xianjun Xu, Leheng Liu, Yulun Wu, Xiaowan Wu, Wenlu Niu, Dandan You, Xiaobo Cai, Lungen Lu, Hui Zhou

“…Immunofluorescence, Immunohistochemistry, Western blot, RT-qPCR, ELISA, 16S rDNA sequencing, tissue and enteroid culture techniques were used to evaluate the effects of an HFD or DCA on the gut‒liver axis in mice or humans. …”
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Ruminal digestion, gastrointestinal microbial profile, and metabolic pathways after the introduction of silicon-containing ultrafine particles into bull by Elena Sizova, Elena Yausheva, Sergey Miroshnikov, Aina Kamirova, Daniil Shoshin

“…Digestibility coefficients were measured using standard methods, and ruminal fluid samples were subjected to 16S ribosomal RNA sequencing and Kyoto encyclopedia of genes and genomes -based functional profiling. …”
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Activity of fungicide cyclobutrifluram against Fusarium fujikuroi and mechanism of the pathogen resistance associated with point mutations in FfSdhB, FfSdhC2 and FfSdhD by Yang Sun, Yu Liu, Li Zhou, Xinyan Liu, Kun Wang, Xing Chen, Chuanqing Zhang, Yu Chen

“…This study aimed to evaluate the potential of cyclobutrifluram, a novel succinate dehydrogenase inhibitor (SDHI), to control RBD, and determine the risk and mechanism of resistance to cyclobutrifluram in F. fujikuroi. …”
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Potential role of Dermanyssus gallinae as a vector of chicken infectious Anemia Virus by Kai Xu, Xuedi Zhang, Zhonghao Wang, Jing Liu, Shuo Yin, Yixin Wang, Jianhua Qin, Chuanwen Wang

“…Two methods of mite infection were then evaluated: blood-feeding and cutaneous contact, and results indicated that D. gallinae was infected by two methods, achieving infection rates of 100 % and 96.6 % within 72 h, respectively. …”
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Histidine metabolism drives liver cancer progression via immune microenvironment modulation through metabolic reprogramming by Pengcheng Liu, Fuxin Huang, Peixu Lin, Jiayao Liu, Pincheng Zhou, Jie Wang, Huanhuan Sun, Fan Xing, Haiqing Ma

“…Methods Using single-cell RNA sequencing, the expression patterns of histidine metabolism–related genes were evaluated across different cell types in HCC samples. …”
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Qiangyang decoction attenuates ischemia/reperfusion-induced cardiomyocyte ferroptosis via ULK1/KEAP1/Nrf2 axis by Jing Wang, Wan Cai, Jingxuan Chen, Wenjin Wang, Wentao Fu, Wen Ge, Hao Chi

“…An in vivo I/R mouse model and an in vitro cardiomyocytes OGD model were utilized to evaluate the cardiac protective effect of QYD. Transcriptome analysis and western blotting assays were used to explore the mechanism. …”
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Mitochondria-targeted antioxidant mitoquinone mitigates vitrification-induced damage in mouse ovarian tissue by maintaining mitochondrial homeostasis via the p38 MAPK pathway by Tianqi Du, Han Su, Dan Cao, Qingxia Meng, Ming Zhang, Zhenxing Liu, Hong Li

“…Autotransplantation experiments were conducted, and the long-term effects of MitoQ on ovarian function were evaluated by counting follicle numbers through H&E staining and measuring serum estradiol and AMH levels using ELISA. …”
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The ADAMTS2 metalloproteinase inhibits tumor growth by regulating the innate immune system by Loïc Joannes, Laura Dupont, Louis Stock, Esther Arpigny, Pascale Hubert, Marie Ancion, Margaux Luyckx, Joan Abinet, Wen Peng, Didier Calaldo, Agnes Noel, Thomas Marichal, Michael Herfs, Christophe Deroanne, Alain Colige

“…This hypothesis was evaluated in vivo using ADAMTS2-KO mice and different tumor models characterized by the absence or presence of tumor collagen accumulation, as in MMTV-PyMT mice which develop spontaneous desmoplastic mammary tumors. …”
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Gut microbiota dysbiosis in patients with Alzheimer’s disease and correlation with multiple cognitive domains by Qionglei Chen, Jiayu Shi, Gaojie Yu, Huijia Xie, Shicheng Yu, Jin Xu, Jiaming Liu, Jing Sun

“…The global cognition, specific cognitive domains (abstraction, orientation, attention, language, etc.) and severity of cognitive impairment, were evaluated by Montreal Cognitive Assessment (MoCA) scores. …”
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Disrupted mitochondrial morphology and function exacerbate inflammation in elderly-onset ulcerative colitis by Mengmeng Zhang, Hong Lv, Xiaoyin Bai, Gechong Ruan, Qing Li, Kai Lin, Hong Yang, Jiaming Qian

“…This study aims to reveal the role of mitochondrial damage in the pathogenesis of adult- and elderly-onset UC. Methods RNA-sequencing of colonic mucosa from adult- and elderly-onset UC patients was performed. …”
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Utility of a Large Series of B‐Cell Precursor Acute Lymphoblastic Leukemia Cell Lines as a Model System by Minori Tamai, Chiaki Komatsu, Keiko Kagami, Shin Kasai, Koshi Akahane, Kumiko Goi, Kanji Sugita, Chihiro Tomoyasu, Toshihiko Imamura, Hiroaki Goto, Takeshi Inukai

“…ABSTRACT Background In B‐cell precursor acute lymphoblastic leukemia (BCP‐ALL), chromosomal translocations are strongly associated with prognoses. RNA sequencing (RNA‐seq) is a powerful technology that reveals a close correlation between types of translocation and patterns of gene expression in clinical samples of BCP‐ALL. …”
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Integrating network pharmacology and multi-omics in a systems approach: a mechanism study of Jinhong tablets against chronic superficial gastritis by Lihao Xiao, Tingyu Zhang, Yun Liu, Chayanis Sutcharitchan, Qingyuan Liu, Xiaoxue Fan, Jian Feng, Huifang Gao, Shao Li, Tong Zhang

“…The study also analyzed rat plasma for differential metabolites through untargeted metabolomics and evaluated the diversity and composition of gut microbiota in fecal and cecal contents samples using 16S rRNA sequencing. …”
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