Prediction of lung adenocarcinoma prognosis and clinical treatment efficacy by telomere-associated gene risk model by Yan Jiang, Jun Liu, Bi Pan, Siping Yu, Chunyan Hu, Qiancheng Li, Hong Cheng, Ling Chen, Min Jiang, Die Xu, Chuanhui Wang, Jie Yan

“…Data pertinent to RNA sequencing and clinical parameters were accessed from two widely-accessed electronic repositories- the GEO and TCGA databases, respectively. …”
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Highly proliferating cancer cells function as novel prognostic biomarkers for lung adenocarcinoma with particular usefulness for stage IA risk stratification by Yanlu Xiong, Yongfu Ma, Jie Lei, Jianfei Zhu, Nianlin Xie, Feng Tian, Qiang Lu, Miaomiao Wen, Qian Zheng, Yong Han, Tao Jiang, Yang Liu

“…Then, we quantified the content of HPCs in nine LUAD datasets by single-sample gene set enrichment analysis and evaluated the relationship between the percentage of HPCs and overall survival (OS). …”
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Developing a predictive model for neoadjuvant therapy in HER2 overexpression breast cancer using multi-parameter MRI radiomics: two-center retrospective study by Lingling Wang, Jingru Yang, Li Yang, Yun Zhu, Xiaomin Tang, Xinyu Cao, Wenbo Kang, Haitao Sun, Zongyu Xie, Zongyu Xie, Zongyu Xie

“…The patients from two centers were split into a training group (n=68) and a test group (n=65). MRI sequences (fs-T2WI, DWI, DCE-MRI) were used to outline regions of interest (ROI). …”
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Transcriptome profiling of Anoectochilus roxburghii L. reveals spermidine-mediated response to drought stress by Muhammad Waheed Riaz, Qingjun Zou, Ailian Zhang, Aicun Zhou, Muhammad Yasir, Chunqing Chen, Ying Zheng, Qingsong Shao

“…Therefore, the objective of this study was to evaluate transcriptomic changes of jewel orchid plants in response to drought and exogenous spermidine application employing RNA sequencing. …”
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Measurable residual disease monitoring in AML with FLT3-ITD treated with intensive chemotherapy plus midostaurin by Frank G. Rücker, Lars Bullinger, Sibylle Cocciardi, Sabrina Skambraks, Tamara J. Luck, Daniela Weber, Julia Krzykalla, Ema Pozek, Isabelle Schneider, Andrea Corbacioglu, Verena I. Gaidzik, Annika Meid, Sophia Aicher, Frank Stegelmann, Anika Schrade, Frauke Theis, Walter Fiedler, Helmut R. Salih, Gerald Wulf, Hans Salwender, Thomas Schroeder, Katharina S. Götze, Michael W. M. Kühn, Michael Lübbert, Richard F. Schlenk, Axel Benner, Felicitas Thol, Michael Heuser, Arnold Ganser, Hartmut Döhner, Konstanze Döhner

“…Using our recently developed FLT3-ITD paired-end next-generation sequencing (NGS)–based MRD assay (limit of detection 10−4 to 10−5), we evaluated the prognostic impact of MRD at different time points in 157 patients with FLT3-ITDpos AML who were enrolled in the German-Austrian Acute Myeloid Leukemia Study Group 16-10 trial and who were treated with a combination of intensive chemotherapy and midostaurin, followed by midostaurin maintenance. …”
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Characterization of <i>Klebsiella pneumoniae</i> Isolates Resistant to Cefiderocol from Hospitals and Outpatient Settings in Croatia by Branka Bedenić, Josefa Luxner, Gernot Zarfel, Ana Benčić, Sanda Sardelić, Maja Anušić, Jasmina Vraneš, Verena Dobretzberger, Ivan Barišić, Andrea Grisold

“…An inter-array genotyping CarbaResist test and whole genome sequencing (WGS) were applied on selected isolates. …”
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Compost Tea Combined with Fungicides Modulates Grapevine Bacteriome and Metabolome to Suppress Downy Mildew by Giuliano Bonanomi, Giuseppina Iacomino, Ayoub Idbella, Giandomenico Amoroso, Alessia Staropoli, Andrea De Sio, Franco Saccocci, Ahmed M. Abd-ElGawad, Mauro Moreno, Mohamed Idbella

“…Restrictions on synthetic pesticides and inconsistent efficacy of current biocontrol agents, especially under rainy conditions, complicate disease management. This study evaluated the potential of compost tea to suppress downy mildew in a two-year field experiment (2023 and 2024), combined with reduced synthetic fungicide applications. …”
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Sirtuin-3 activation by honokiol attenuated anesthesia/surgery-induced cognitive impairment and neuronal ferroptosis via inhibiting mitochondrial GPX4 acetylation by Lian Zeng, Pengchao Hu, Xuan Wang, Xudong Ding, Qingsong Wang, Li Luo, Yu Zhang, Mingyue Li, Yilin Zhao, Shiyong Li, Ailin Luo

“…Mice were treated with Honokiol (HKL) or erastin to evaluate hippocampal ferroptosis. RNA sequencing (RNA-seq) was performed to identify the underlying neuroprotective mechanisms. …”
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Analytical and clinical validation of a novel MeltPlus TB-NTM/RIF platform for simultaneous detection of Mycobacterium tuberculosis complex, Non-Tuberculous Mycobacteria and rifamp... by Zhuo Wang, Yuanwu Zou, Zihan Wei, Guanghong Bai, Xiaolin Wang, Shaoyi Qu, Jie Shi, Yaping Jiang, Cuijiao Gu

“…BackgroundRapid and accurate diagnosis of tuberculosis, particularly rifampin (RIF)-resistant tuberculosis (RR-TB) and Non-Tuberculous Mycobacteria (NTM), is essential for implementing appropriate proper therapy to benefit patients and improve TB/NTM patient management.MethodsIn this study, we developed a novel MeltPlus MTB-NTM/RIF platform, designed to simultaneously detect Mycobacterium tuberculosis complex (MTBC), NTM and RIF resistance. The platform was evaluated for its limit of detection (LOD) and specificity before clinical validation, followed by a prospective single-center study in patients with presumptive TB cases.ResultsThe calculated LOD for MTBC, NTM and RIF susceptibility was found to be 10.31 CFU/mL, 57.55 CFU/mL and 48.584 CFU/mL, respectively. …”
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Machine learning-based integration identifies plasma cells-related gene signature ST6GAL1 in idiopathic pulmonary fibrosis by Fanjie Lin, Ken Lin, Donglei Li, Weiguo Kong, Jiayu Zhuang, Wei He, Xinguang Wei, Tianchi Xiao, Hao Zu, Zili Zhang, Wenju Lu

“…Conclusions PCRGS might be used to evaluate the IPF prognosis, among which ST6GAL1 is a potential therapeutic target. …”
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Establishment of a prognostic model based on ER stress-related cell death genes and proposing a novel combination therapy in acute myeloid leukemia by Minghui Wang, Huajian Xian, Xiaoli Xia, Wenjie Zhang, Zixuan Huang, Chaoqun Lu, Yuling Zheng, Yixin Wang, Shufeng Xie, Renyao Pan, YaoYifu Yu, Ruiheng Wang, Huijian Zheng, Guorui Huang, Han Liu

“…Methods We analyzed RNA sequencing and clinical data from 887 AML patients across 4 cohorts to develop an ER stress-related cell death index (ERCDI) using 10 machine-learning algorithms with 117 unique combinations. …”
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Genomic epidemiology and anti-biofilm mechanisms of Lactobacillus in ST11 carbapenem-resistant Klebsiella pneumoniae in China by Shuang Li, Duo Zhang, Duo Zhang, Xindan Zhang, Xindan Zhang, Xiaohan Ma, Xiaohan Ma, Shuai Zheng, Shuai Zheng, Di Zhou, Di Zhou, Qinlong Hou, Qinlong Hou, Gen Li, Gen Li, Huiming Han, Huiming Han

“…A total of 2,805 clinical isolates were screened, and 334 ST11 strains were identified using MALDI-TOF mass spectrometry and whole-genome sequencing. Biofilm formation was assessed through microtiter plate assays, while co-culture experiments with Lactobacillus fermentum and Lactobacillus gasseri were performed to evaluate anti-biofilm activity. …”
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A Mouse Model of Mild <i>Clostridioides difficile</i> Infection for the Characterization of Natural Immune Responses by Assaf Mizrahi, Gauthier Péan de Ponfilly, Diane Sapa, Antonia Suau, Irène Mangin, Aurélie Baliarda, Sandra Hoys, Benoît Pilmis, Sylvie Lambert, Anaïs Brosse, Alban Le Monnier

“…Gut microbiota dysbiosis was confirmed and described by 16S rDNA sequencing. We sacrificed C57Bl/6 mice after different stages of infection (day 6, 2, 7, 14, 21, 28, and 56) to evaluate IgM, IgG against TcdA, TcdB, SlpA, FliC in blood samples, and IgA in the cecal contents collected. (3) Results: In our model, we observed a reproducible gut microbiota dysbiosis, allowing for <i>C. difficile</i> digestive colonization. …”
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Steroid-Resistant Nephrotic Syndrome Is Associated With a Unique Genetic Profile in a Highly Admixed Pediatric Population by Andreia Watanabe, Precil Diego Miranda de Menezes Neves, Kelly Nunes, Antonio Marcondes Lerario, Elieser Hitoshi Watanabe, Frederico Moraes Ferreira, Denise Maria Avancini Costa Malheiros, Amanda de Moraes Narcizo, Mara Sanches Guaragna, Stanley de Almeida Araujo, Thais Medeiros Cruz, Jussara Soares Fontes, Vera Maria Santoro Belangero, Maria Helena Vaisbich, Friedhelm Hildebrandt, Matthew Gordon Sampson, Luiz Fernando Onuchic

“…Methods: A total of 101 pediatric patients with primary SRNS were genetically assessed targeting Mendelian causes and APOL1 status with a 62-NS-gene panel or whole exome sequencing, as well as genetic ancestry. Variant pathogenicity was evaluated using the American College Medical of Genetics and Genomics (ACMG) criteria. …”
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Characterization and Potential Application of Phage vB_PmuM_CFP3 for Phage Therapy Against Avian <i>Pasteurella multocida</i> by Hongmei Chen, Nansong Jiang, Guanghua Fu, Qiuling Fu, Chunhe Wan, Yu Huang, Yuan Liu, Rongchang Liu, Qizhang Liang, Longfei Cheng

“…While CFP3 shows promise as a precision therapeutic tool, further in vivo studies are required to evaluate its efficacy and safety. Future research should focus on expanding the phage library, optimizing phage mixtures, and exploring synergistic effects with other antimicrobial strategies. …”
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Nanostructured lipid carriers based mRNA vaccine leads to a T cell–inflamed tumour microenvironment favourable for improving PD-1/PD-L1 blocking therapy and long-term immunity in a... by Carole Fournier, Marion Mercey-Ressejac, Valentin Derangère, Amal Al Kadi, David Rageot, Christine Charrat, Alexis Leroy, Julien Vollaire, Véronique Josserand, Marie Escudé, Séverine Escaich, François Ghiringhelli, Thomas Decaens, Fabrice P. Navarro, Evelyne Jouvin-Marche, Patrice N. Marche

“…The intratumoral vaccine-related gene signature was assessed by RNA-sequencing. The immune memory response was assessed by re-challenging surviving treated mice with tumour cells. …”
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Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds by Yun-feng LU, Ji-bao CHEN, Bo ZHANG, Qing-gang LI, Zhi-xiu WANG, Hao ZHANG, Ke-liang WU

“…Single factor linear correlation analysis was applied separately for each breed to evaluate correlations between ECI1 gene expression in the LD muscle and intramuscular fat (IMF) content. …”
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Phenotypic and genotypic characteristics of children with Bartter syndrome by Serçin Güven, Pınar Ata, Nurdan Yıldız, Özde Nisa Türkkan, Serim Pul, Mehtap Sak, Ece Bodur Demirci, Neslihan Çiçek, Ceren Alavanda, İbrahim Gökçe, Harika Alpay

“…In the present study, target gene sequencing was performed to evaluate the genotype-phenotype relationship. …”
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Magnetic Resonance Imaging in Multiple Sclerosis: An Analysis of the Implementation of the Portuguese Consensus by Ricardo Soares dos Reis, Alyne Cordeiro, Ana João Marques, Ana Rita Castro, Cristiana Silva, Duarte Carapinha, Francisca Ferreira, Miguel Schön, Daniela Jardim Pereira, João Paulo Gabriel, Luís Ruano, Mariana Santos, Miguel Grunho, Mónica Santos, José Vale, Maria José Sá

“…The 2020 Portuguese Consensus on Magnetic Resonance Imaging in Multiple Sclerosis aimed to standardize MRI use. This study evaluated the implementation of the consensus in clinical practice. …”
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Single-Cell RNA Transcriptomics and Multi-omics Analyses Reveal the Clinical Effects of Acupuncture on Methadone Reduction by Yiming Chen, Baochao Fan, Jingchun Zeng, Yutian Zou, Chenyang Tao, Chen Chen, Peiming Zhang, Jian Liang, Fangfang Qi, Hailin Tang, Liming Lu

“…Therefore, we aimed to integrate clinical trial data with multi-omics analysis, including single-cell sequencing, transcriptomics, metabolomics, and metagenomics, to evaluate the effects of acupuncture in patients undergoing MMT. …”
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