Effect of DNA methylation on inhibitor development in people with hemophilia A treated with FVIII concentrates by Himani Chand, Shermarke Hassan, Andrea Cairo, Roberta Palla, Flora Peyvandi, Flora Peyvandi, Pier M. Mannucci, Isabella Garagiola, Amal El-Beshlawy, Mohsen Elalfy, Vijay Ramanan, Peyman Eshghi, Suresh Hanagavadi, Ramabadran Varadarajan, Mehran Karimi, Mamta V. Manglani, Cecil Ross, Guy Young, Tulika Seth, Shashikant Apte, Dinesh M. Nayak, Elena Santagostino, Maria Elisa Mancuso, Adriana C. Sandoval Gonzalez, Johnny N. Mahlangu, Santiago Bonanad Boix, Monica Cerqueira, Nadia P. Ewing, Christoph Male, Tarek Owaidah, Veronica Soto Arellano, Nathan L. Kobrinsky, Suvankar Majumdar, Rosario Perez Garrido, Anupam Sachdeva, Mindy Simpson, Mathew Thomas, Ezio Zanon, Bulent Antmen, Kaan Kavakli, Marilyn J. Manco-Johnson, Monica Martinez, Esperanza Marzouka, Maria G. Mazzucconi, Daniela Neme, Angeles Palomo Bravo, Rogelio Paredes Aguilera, Alessandra Prezotti, Klaus Schmitt, Brian M. Wicklund, Bulent Zulfikar, Frits R. Rosendaal

“…Enrichment bisulfite sequencing was performed on DNA samples from PBMCs of HA patients and differentially methylated CpG sites (DMCs) were identified with bioinformatic approach. …”
Get full text

Immune-phenotyping and transcriptomic profiling of blood monocytes from patients with breast cancer under neoadjuvant chemotherapy by M. R. Patysheva, M. N. Stakheyeva, E. S. Grigoryeva, P. S. Iamshchikov, I. V. Larionova, А. A. Budnickya, N. A. Tarabanovskaya, N. V. Cherdyntseva, J. G. Kzhyshkowska

“…Bulk RNA sequencing was adopted to explore the transcriptomic profile of CD14+ monocytes before and after treatment. …”
Get full text

Yeast Fermentate Prebiotic Ameliorates Allergic Asthma, Associating with Inhibiting Inflammation and Reducing Oxidative Stress Level through Suppressing Autophagy by Subo Gong, Xiaoying Ji, Jing Su, Yina Wang, Xianghong Yan, Guyi Wang, Bin Xiao, Haiyun Dong, Xudong Xiang, Shaokun Liu

“…The pathological state, tight-junction proteins, inflammatory and oxidative stress-associated biomarkers, and TLRs/NF-κB signaling pathway of the lung tissues were evaluated by HE staining, immunofluorescence, ELISA, and WB, separately. …”
Get full text

Targeting FGFR4 abrogates HNF1A-driven metastasis in pancreatic ductal adenocarcinoma by Katherine J. Crawford, Kennedy S. Humphrey, Eduardo Cortes, Jianxin Wang, Vishnu Kumarasamy, Yin Wan, Mark D. Long, Michael E. Feigin, Agnieszka K. Witkiewicz, Karen M. Mann, Erik S. Knudsen, Ethan V. Abel

“…An intrasplenic injection xenograft model was used to evaluate the impact of HNF1A knockdown and overexpression on metastatic tumor burden. …”
Get full text

A novel lncRNA FLJ promotes castration resistance in prostate cancer through AR mediated autophagy by Yingying Wu, Shaojie Cheng, Ting Zhang, Leilei Wang, Ting Li, Yongbo Zheng, Guo Yang, Xiaohou Wu, Chunli Luo, Tingmei Chen, Liping Ou

“…Methods Firstly, we explore the CRPC associated lncRNAs by RNA sequencing and validated using quantitative polymerase chain reaction (qRT-PCR) and RNA fluorescence in situ hybridization (RNA-FISH). …”
Get full text

Enhancing antitumor immunity through the combination of cholesterolized TLR7 agonist liposomes and radiotherapy: a role for IL‐1β and the inflammasome pathway by Xuejiao Han, Yuan Cheng, Dandan Wan, Aqu Alu, Ziqi Zhang, Zhenfei Bi, Manni Wang, Yan Tang, Weiqi Hong, Siyuan Chen, Li Chen, Yuquan Wei

“…The involvement of interleukin‐1β (IL‐1β) and the inflammasome pathway in the antitumor efficacy of the combined treatment was evaluated using Il‐1β−/− and cysteinyl aspartate specific proteinase 1 knockout (Casp1−/−) mouse models. …”
Get full text

Integrated identification of immune-related therapeutic targets for interstitial cystitis via multi-algorithm machine learning: transcriptomic profiling and in vivo experimental va... by Yifan Wang, Chuanzan Zhou, Facai Zhang, Yunkai Yang, Jia Miao, Xuanhan Hu, Xinyu Zhang, Alin Ji, Qi Zhang

“…Immune cell infiltration was assessed via CIBERSORT, and single cell sequencing elucidated cellular heterogeneity. Drug candidates were predicted using DSigdb and validated through molecular docking and dynamics simulations. …”
Get full text

Use of approved cefazolin based intramammary dry goat treatment and its effects on the milk microbiota by Delower Hossain, Filippo Biscarini, Valentina Monistero, Paola Cremonesi, Bianca Castiglioni, Anoar Jamai Masroure, Alessandro Marinoni, Sara Fusar Poli, Matteo Mezzetti, Maria Filippa Addis, Valerio Bronzo, Erminio Trevisi, Paolo Moroni

“…Therefore, this study was designed to evaluate the effect of an approved cefazolin-based intramammary treatment on the milk microbiota of Alpine dairy goats during the dry and early lactation periods. …”
Get full text

Genetic and transcriptomic analyses of early-onset colon cancer (EOCC): a post hoc analysis of 2973 patients from two adjuvant randomized trials by A. Gandini, C. Gallois, H. Blons, C. Mulot, N. Agueeff, C. Lepage, R. Guimbaud, L. Mineur, J. Desramé, B. Chibaudel, A. de Reyniès, T. André, P. Laurent-Puig, J. Taieb

“…RNA-sequencing analyses were carried out to determine consensus molecular subtypes (CMS). …”
Get full text

Lactate Facilitates Pancreatic Repair Following Acute Pancreatitis by Promoting Reparative Macrophage PolarizationSummary by Jing Jiang, Ruiyan Wang, Pengli Song, Qi Peng, Xuerui Jin, Bin Li, Jianbo Ni, Jie Shen, Jingpiao Bao, Zengkai Wu, Xiaolu Ge, Xingpeng Wang, Guoyong Hu

“…Pancreatic repair was evaluated histologically, whereas lactate levels and inflammatory markers were measured serologically. …”
Get full text

Trypanosoma dionisii in China: ecology and tentative epidemiology by Qin Liu, Mu-xin Chen, Yu-chun Cai, Yuan-yuan Li, Zi-yi Wang, Yun-hai Guo, Yu-wan Hao, Jing-bo Xue, Yi-dan Jing, Fan-na Wei, Yong-bin Wang, Yue-jin Li, Hai-fang Wang, Jun-ling Sun, Ya-li Wang, Gang Wang, Na Wang, Nai-li Guo, Jian-cun Fang, Wei-xiao Chen, Xun-ming Zhou, Yang Yu, Yi Zhang, Jun-hu Chen, Qiang Wang, Shi-zhu Li, Ge Yan, Qun Li

“…Further surveillance and analysis are warranted to evaluate the transmission risk. Graphical Abstract…”
Get full text

The Effects of Microplastics and Heavy Metals Individually and in Combination on the Growth of Water Spinach (<i>Ipomoea aquatic</i>) and Rhizosphere Microorganisms by Jing-Yi Wang, Meng Wang, Jian-Wei Shi, B. Larry Li, Ling Liu, Peng-Fei Duan, Zhao-Jin Chen

“…The impacts on water spinach (<i>Ipomoea aquatica</i>) growth and heavy metal accumulation were evaluated, and the rhizosphere bacterial and fungal community structure and diversity were analyzed using high-throughput sequencing. …”
Get full text

Protocol for a randomised ‘screen-and-treat’ Helicobacter pylori eradication trial in 14–18-years-old adolescents residing in three regions of Chile: effectiveness and microbiologi... by Ximena Aguilera, Camila Cabrera, Sergio George, Yalda Lucero, Beatriz Zabala Torres, Lilian Fernández, Nora Mamani, Anne Lagomarcino, Miguel O'Ryan

“…Enzyme-linked immunosorbent assay (ELISA) commercial kits will evaluate gastric damage biomarkers in serum (pepsinogen I and II, gastrin-17, VCAM-1, CXCL13). …”
Get full text

Therapeutic gene targets and epigenetic modifications in rheumatoid arthritis: Insights from MTX, JAK inhibitors, and LLDT-8 by Jianan Zhao, Yunshen Li, Runrun Zhang, Yu Shan, Chenyang Song, Yaxin Cheng, Yiming Shi, Yixin Zheng, Fuyu Zhao, Junyu Fan, Cen Chang, Yuejuan Zheng, Dongyi He

“…Methylation changes in key genes were examined using the GEO database (GSE46364), and clinical relevance was evaluated using RA patient data from the PEAC database. …”
Get full text

NORDIC denoising on VASO data by Lasse Knudsen, Lasse Knudsen, Luca Vizioli, Federico De Martino, Lonike K. Faes, Daniel A. Handwerker, Steen Moeller, Peter A. Bandettini, Peter A. Bandettini, Laurentius Huber

“…We present a preliminary analysis to evaluate NORDIC’s capability to suppress thermal noise while preserving the VASO signal across a wide parameter space at 3T. …”
Get full text

Multimodal sleep staging network based on obstructive sleep apnea by Jingxin Fan, Jingxin Fan, Jingxin Fan, Mingfu Zhao, Li Huang, Li Huang, Bin Tang, Bin Tang, Lurui Wang, Zhong He, Zhong He, Xiaoling Peng

“…MSDC-SSNet transforms two channels of electroencephalogram (EEG) and one channel of electrooculogram (EOG) signals into time-frequency representations to obtain feature sequences at different temporal and frequency scales. …”
Get full text

Identification of highly expressed genes and efficient core promoters specific to buffalo skeletal muscles by J. Huang, J. Huang, D. Guo, R. Zhu, H. Wang, C. Yang, D. Shi, J. Shang

“…Then, the transcriptional activity of different truncated fragments of the upstream putative promoter region of<i> VGLL2</i> and <i>CACNG1</i> were evaluated by the dual luciferase reporter gene detection system in mouse C2C12 cells and buffalo skeletal muscle cells. …”
Get full text

<i>Bacillus licheniformis</i> Alleviates <i>Clostridium perfringens</i>-Induced Intestinal Injury in Mice Model by Modulating Inflammation, Apoptosis, and Cecal Microbial–Metabolic... by Yifan Zhong, Meiting Zhang, Haocheng Xu, Xiaorong Yu, Yashi Hu, Yangyi Xu, Xiao Xiao, Caimei Yang

“…This study aimed to evaluate the protective effects and underlying mechanisms of <i>B. licheniformis</i> in a mouse model challenged with <i>Clostridium perfringens</i> (<i>C. perfringens</i>). …”
Get full text

Modulation of muscle anabolism and gut microbiota by Polyporus umbellatus extract attenuates dexamethasone-induced muscle atrophy by Nguyen Bao Ngoc, Subeen Kim, Hye-Young Youn, Huitae Min, Tam Thi Le, Mauliasari Intan Rizki, Dae Won Kim, Kwang Hyeon Cha, Young Tae Park, Sang Hoon Jung, Myungsuk Kim

“…Gut microbiota composition was analyzed via 16 S rRNA sequencing, with predicted microbial enzyme functions and correlations to muscle parameters examined. …”
Get full text

Clinical-radiomics hybrid modeling outperforms conventional models: machine learning enhances stratification of adverse prognostic features in prostate cancer by Minghan Jiang, Minghan Jiang, Zeyang Miao, Run Xu, Mengyao Guo, Xuefeng Li, Guanwu Li, Peng Luo, Su Hu, Su Hu

“…Regions of interest (ROIs) were delineated on ADC and T2WI sequences, and 31 radiomics features were extracted using PyRadiomics. …”
Get full text