Natural lung-tropic TH9 cells: a sharp weapon for established lung metastases by Tao Chen, Hao Zhou, Shuming Li, Nong Lin, Yixuan Feng, Zhijian Cai, Chenxiao Qiao, Eloy Yinwang, Shengdong Wang, Xuehuan Wen, Xiangang Jin, Yucheng Xue, Wenkan Zhang, Xianchang Zeng, Zenan Wang, Hangxiang Sun, Lifeng Jiang, Hengyuan Li, Binghao Li, Zhaoming Ye

“…We employed flow cytometry and an in vivo imaging system to evaluate the accumulation patterns of TH1, TH9, and TH17 cells in the lungs after transfusion. …”
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T cell memory response to MPXV infection exhibits greater effector function and migratory potential compared to MVA-BN vaccination by Ji-Li Chen, Beibei Wang, Yongxu Lu, Elie Antoun, Olivia Bird, Philip G. Drennan, Zixi Yin, Guihai Liu, Xuan Yao, Maya Pidoux, Adam Bates, Deshni Jayathilaka, Junyuan Wang, Brian Angus, Sally Beer, Alexis Espinosa, J. Kenneth Baillie, Malcolm G. Semple, ISARIC4C Investigators, Timothy Rostron, Craig Waugh, Paul Sopp, Julian C. Knight, James N. Fullerton, Mark Coles, Geoffrey L. Smith, Alexander J. Mentzer, Yanchun Peng, Tao Dong

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Cold storage of human precision-cut lung slices in TiProtec preserves cellular composition and transcriptional responses and enables on-demand mechanistic studies by M. Camila Melo-Narvaez, Fee Gölitz, Eshita Jain, Janine Gote-Schniering, Mircea Gabriel Stoleriu, Wilhelm Bertrams, Bernd Schmeck, Ali Önder Yildirim, Ursula Rauen, Timo Wille, Mareike Lehmann

“…Moreover, bulk-RNA sequencing was used to study transcriptional changes, regulated signaling pathways, and cellular composition after cold storage. …”
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Oncolytic Orf virus licenses NK cells via cDC1 to activate innate and adaptive antitumor mechanisms and extends survival in a murine model of late-stage ovarian cancer by Jacob P van Vloten, Kathy Matuszewska, Mark A A Minow, Jessica A Minott, Lisa A Santry, Madison Pereira, Ashley A Stegelmeier, Thomas M McAusland, Elaine M Klafuric, Khalil Karimi, Joseph Colasanti, D Grant McFadden, James J Petrik, Byram W Bridle, Sarah K Wootton

“…Immune cell profiling, impact on secondary lesion development and survival were evaluated in OrfV-treated mice as well as in Batf3 knockout, mice depleted of specific immune cell subsets and in mice where the primary tumor was removed. …”
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Neoepitope BTLAP267L-specific TCR-T cell immunotherapy unlocks precision treatment for hepatocellular carcinoma by Fang Liu, Hua Chen, Suxin Wu, Chenlu Zhu, Mingji Zhang, Wei Rui, Dong Zhou, Yang Wang, Xin Lin, Xueqiang Zhao, Yunbin Ye

“…The cytotoxicity of CD8+ TCR-T cells was evaluated in neoepitope-positive tumor cell lines or NCG mice. …”
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Immune profiling of the macroenvironment in colorectal cancer unveils systemic dysfunction and plasticity of immune cells by Haoxian Ke, Peisi Li, Zhihao Li, Xian Zeng, Chi Zhang, Shuzhen Luo, Xiaofang Chen, Xinlan Zhou, Shichen Dong, Shaopeng Chen, Junfeng Huang, Ming Yuan, Runfeng Yu, Shubiao Ye, Tuo Hu, Zhonghui Tang, Dongbin Liu, Kui Wu, Xianrui Wu, Ping Lan

“…Immunoprofiling was also validated by a validation cohort using single‐cell RNA sequencing, spatial transcription, CyTOF and multiplex immunofluorescent staining. …”
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D-Psicose mitigates NAFLD mice induced by a high-fat diet by reducing lipid accumulation, inflammation, and oxidative stress by Jiajun Tan, Jiajun Tan, Wen Sun, Xueyun Dong, Jiayuan He, Asmaa Ali, Asmaa Ali, Min Chen, Leilei Zhang, Liang Wu, Liang Wu, Keke Shao, Keke Shao

“…To explore its underlying mechanisms, colon contents 16S rRNA sequencing and serum untargeted metabolomics were performed. …”
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Identification of galectin-9 and its antibacterial function in Yellow River carp (Cyprinus carpio haematopterus) by Li Wang, Li Wang, Li Wang, Yuanyuan Hu, Xudong Li, Ziyan Zhang, Nan Wang, Limin Chao, Chengfei Li, Pei Gao, Jinyou Ma, Jinyou Ma, Lei Wang, Xiaojing Xia, Xiaojing Xia

“…In this study, we identified and characterized a novel galectin-9 cDNA, designated CcGal-9, from Yellow River carp (Cyprinus carpio haematopterus).MethodsThe full-length CcGal-9 cDNA was cloned and sequenced, and its structural features were analyzed. …”
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EIF4E-mediated biogenesis of circPHF14 promotes the growth and metastasis of pancreatic ductal adenocarcinoma via Wnt/β-catenin pathway by Zhou Fang, Zhuo Wu, Chao Yu, Qingyu Xie, Liangtang Zeng, Rufu Chen

“…Methods CircPHF14 (hsa_circ_0079440) was identified through the analysis of RNA sequencing data from PDAC and normal adjacent tissues. …”
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Maternal exposure to per- and polyfluoroalkyl substances and epitope level antibody response to vaccines against measles and rubella in children from the Boston birth cohort by Xiumei Hong, William R. Morgenlander, Kari Nadeau, Guoying Wang, Pamela A. Frischmeyer-Guerrerio, Colleen Pearson, William G. Adams, Hongkai Ji, H. Benjamin Larman, Xiaobin Wang

“…Quantile g-computation was applied to evaluate the PFAS mixture – VARscore associations. …”
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Comprehensive immunophenotyping of gastric adenocarcinoma identifies an inflamed class of tumors amenable to immunotherapies by Felip Vilardell, Xavier Matias-Guiu, Robert Montal, Mar Iglesias, Maria Santacana, María Ruiz, Genis Parra, Joel Veas Rodriguez, Miquel Piñol, Maria Alba Sorolla, Eva Parisi, Anabel Sorolla, Mario Bernabeu, Carles Aracil, Alfredo Escartin, Antonieta Salud

“…A 10-gene RNA signature was developed for the identification of tumors belonging to these classes, demonstrating in evaluated datasets comparable clinical utility in predicting response to current immunotherapies when tested against other published gene signatures.Conclusions Comprehensive immunophenotyping of GAC identifies an inflamed class of tumors that complements previously proposed tumor-based molecular clusters. …”
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Combining SKY92 gene expression profiling and FISH (according to R2‐ISS) defines ultra‐high‐risk Multiple Myeloma by Xiang Zhou, Annika Hofmann, Benedict Engel, Cornelia Vogt, Silvia Nerreter, Yoko Tamamushi, Friederike Schmitt, Maria leberzammer, Emilia Stanojkovska, Marietta Truger, Xianghui Xiao, Christine Riedhammer, Maximilian J. Steinhardt, Mara John, Julia Mersi, Seungbin Han, Umair Munawar, Johannes M. Waldschmidt, Claudia Haferlach, Hermann Einsele, Leo Rasche, K. Martin Kortüm

“…Additionally, whole genome sequencing (WGS) revealed CRBN mutation (n = 3) and bi‐allelic events (mutation and/or deletion) in TP53 (n = 7) and TNFRSF17 (n = 1). …”
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A tumor-infiltrating B lymphocytes -related index based on machine-learning predicts prognosis and immunotherapy response in lung adenocarcinoma by Jiale Fang, Siyuan Yu, Wei Wang, Wei Wang, Cheng Liu, Xiaojia Lv, Jiaqi Jin, Xiaomin Han, Xiaomin Han, Xiaomin Han, Fang Zhou, Yukun Wang

“…We used multiple algorithms to evaluate the relationships between BRI and TIME, as well as immune therapy-related biomarkers. …”
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Efficacy of yeast beta-glucan 1,3/1,6 supplementation on respiratory infection, fatigue, immune markers and gut health among moderate stress adults in Klang Valley of Malaysia: pro... by Suzana Shahar, Kalavathy Ramasamy, Norhayati Ibrahim, Nur Nadia Mohamad Habibullah, Munirah Ismail, Mohd Zul Amin Kamaruddin, Shirley Gee Hoon Tang, Mohd Faisal Abdul Hamid

“…Secondary outcome includes gut microbiota analysis using stool samples via 16S rRNA sequencing.Ethics and dissemination The research protocol of the study was reviewed and approved by the Research Ethics Committee of Universiti Kebangsaan Malaysia (UKM/PPI/111/8/JEP-2023–211). …”
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Molecular classification of hepatocellular carcinoma based on zoned metabolic feature and oncogenic signaling pathway by Tomoko Aoki, Naoshi Nishida, Yutaka Kurebayashi, Kazuko Sakai, Naoto Fujiwara, Masakatsu Tsurusaki, Kohei Hanaoka, Masahiro Morita, Hirokazu Chishina, Masahiro Takita, Satoru Hagiwara, Hiroshi Ida, Kazuomi Ueshima, Yasunori Minami, Atsushi Takebe, Takaaki Murase, Keiko Kamei, Takuya Nakai, Ippei Matsumoto, Kazuto Nishio, Masatoshi Kudo

“…Methods Comprehensive RNA/DNA sequencing, immunohistochemistry, and radiological evaluations were performed on HCC tissues from the training cohort (n=136) and validated in 916 public samples. …”
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A Comprehensive Analysis of Epoxide Hydrolase 2 (EPHX2) in Pan‐Cancer by Weiquan Hu, Xiaoli Ding, Xiangsheng Wu, Xuxiang Xi, Jing Xu, Shengyun Dai, Jing Chen, Suping Hu, Qinfei Zhao, Fangfang Chen

“…Methods Herein, multiple bioinformatics tools were used to comprehensively evaluate the expression, diagnostic, and prognostic significance of EPHX2 and its roles in the tumor immune microenvironment in human cancers. …”
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SRSF1-mediated alternative splicing regulates bladder cancer progression and cisplatin sensitivity through HIF1A/BNIP3/mitophagy axis by Qikai Wu, Hao Yu, Huanyou Sun, Jiancheng Lv, Juntao Zhuang, Lingkai Cai, Lingjing Jiang, Yuhan Chen, Yiran Tao, Kexin Bai, Haiwei Yang, Xiao Yang, Qiang Lu

“…Subsequent RNA sequencing validated the relationship between SRSF1 alternative splicing and the mitophagy pathway. …”
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Pre-conditioning with PQQ during pregnancy alleviates LPS-induced placental damage and improves the fetal survival and growth in mice by Yongli Han, Yan Zhu, Xiaohui Lu, Tingting Fan, Yina Yin, Pengfei Liu, Xiuliang Dai, Hongbin Xu

“…Placental structure, vascular networks, inflammation, oxidative stress, and gene expression profiles were evaluated using H&E staining, immunohistochemistry, Prussian blue staining, and RNA sequencing.ResultsPre-conditioning with PQQ significantly alleviated LPS-induced fetal demise and reduced fetal and placental growth. …”
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Antimicrobial Activity, Genetic Diversity and Safety Assessment of Lactic Acid Bacteria Isolated from European Hakes (<i>Merluccius merluccius</i>, L.) Caught in the Northeast Atla... by Lara Díaz-Formoso, Diogo Contente, Javier Feito, Belén Orgaz, Pablo E. Hernández, Juan Borrero, Estefanía Muñoz-Atienza, Luis M. Cintas

“…Subsequently, their taxonomic classification and genetic diversity were determined by <i>16SrDNA</i> sequencing and Enterobacterial Repetitive Intergenic Consensus-PCR (ERIC-PCR), respectively. …”
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Effects of adding a kind of compound bio-enzyme to the diet on the production performance, serum immunity, and intestinal health of Pekin ducks by Yuxiao Li, Jie Zhou, Tong Guo, Huiya Zhang, Chang Cao, Yingjie Cai, Jiqiao Zhang, Tao Li, Jianqin Zhang

“…This study aimed to evaluate the impact of a kind of compound bio-enzyme supplementation (the main functional components are probiotics and astragalus polysaccharides) on the production performance, serum immunity, and intestinal health of Pekin ducks. …”
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