Molecular genotyping of multi-system rare blood types in foreign blood donors based on DNA sequencing and its clinical significance by Gong Jianli, Xu Xianguo, Zhu Jianrong

“…To establish a multi-level blood type identification system, comprehensively analyze the distribution characteristics and genetic polymorphisms of multi-system rare blood types in foreign blood donors, explore the application value of DNA sequencing technology in rare blood type screening, and evaluate its clinical significance in complex transfusion patients.…”
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Diagnostic Performance of Magnetic Resonance Sequences in Staging Lymph Node Involvement and Extranodal Extension in Head and Neck Squamous Cell Carcinoma by Giovanni Lorusso, Nicola Maggialetti, Francesca Laugello, Annalisa Garofalo, Ilaria Villanova, Sara Greco, Chiara Morelli, Pasquale Pignataro, Nicola Maria Lucarelli, Amato Antonio Stabile Ianora

“…<b>Objectives:</b> This study aimed to evaluate the diagnostic performance of various MRI sequences in detecting nodal metastasis (N+) and extranodal extension (ENE+) in patients with head and neck squamous cell carcinoma (HNSCC). …”
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Saline gargle collection method is comparable to nasopharyngeal/oropharyngeal swabbing for the molecular detection and sequencing of SARS-CoV-2 in Botswana by Kwana Lechiile, Sikhulile Moyo, Mai-Lei Woo Kinshella, Wonderful T. Choga, Leabaneng Tawe, Jonathan Strysko, Gofaone Bagatiseng, Iryna Kayda, Kedumetse Seru, Boitumelo J. L. Zuze, Patience Motshosi, Mosepele Mosepele, Irene Gobe, Simani Gaseitsiwe, Margaret Mokomane, David M. Goldfarb

“…We performed a prospective cross-sectional study to evaluate the SG collection method against the NOS collection method for molecular detection and next-generation sequencing (NGS) of SARS-CoV-2 in Botswana. …”
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Comparative in vitro activities of eravacycline and tigecycline against carbapenem-resistant Acinetobacter baumannii: Insights from whole genome sequencing analysis by Yang Zhong, Jocelyn Qi Min Teo, Hong Yi Chang, Jiahao Yeo, Shuhua Thong, Sihui Tan, Nurhayati Binte Monhamed Yusoff, Tze Peng Lim, Andrea Lay Hoon Kwa

“…ABSTRACT: Objectives: Eravacycline (ERV), a novel tetracycline-class antibiotic, has demonstrated efficacy against carbapenem-resistant Acinetobacter baumannii (CRAB) in Europe and the United States but has not been evaluated in Singapore. The objectives of this study are to evaluate the activity of ERV against CRAB clinical isolates from Singapore and to investigate the genetic profiles of these isolates, exploring novel potential ERV resistance mechanisms through whole genome sequencing (WGS) analysis. …”
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Clinical diagnostic value of throat swabs in pediatric acute lower respiratory tract infections using targeted next-generation sequencing by Di Lian, Chenye Lin, ZhiNan Zhang, JianXing Wei, Dong Wang, QiuYu Tang

“…Abstract Background To evaluate the clinical utility of targeted next-generation sequencing (tNGS) for pathogen detection of pediatric acute lower respiratory tract infections (ALRTIs), with a particular focus on the use of throat swab samples. …”
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Single-cell RNA sequencing combined with proteomics of infected macrophages reveals prothymosin-α as a target for treatment of apical periodontitis by Qimei Gong, Xiaomin Lv, Chenxi Liao, Ailin Liang, Cuiting Luo, Jie Wu, Yanling Zhou, Yihua Huang, Zhongchun Tong

“…Objectives: We aim to explore the heterogeneity of monocyte populations in periapical lesion of CAP tissues and healthy control (HC) periodontal tissues by single-cell RNA sequencing (scRNA-seq), search novel targets for alleviating CAP, and further validate it by proteomics and in vitro and in vivo evaluations. …”
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Comparison of CT-like MRI sequences for preoperative planning of cochlear implantation using super-high-resolution CT as a reference by Ulysse Puel, Achille Beysang, Gabriella Hossu, Michael Eliezer, Bouchra Assabah, Khalid Ambarki, Pedro Augusto Gondim Teixeira, Alain Blum, Cécile Parietti-Winkler, Romain Gillet

“…Abstract Background We evaluated the accuracy of magnetic resonance imaging (MRI) computed tomography (CT)-like sequences compared to normal-resolution CT (NR-CT) and super-high-resolution CT (SHR-CT) for planning of cochlear implantation. …”
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High-speed spin measurement system for dotted table tennis ball using single-frame M-sequence multi-exposures by Tomohiro Sueishi, Himari Tochioka, Masatoshi Ishikawa

“…In this paper, we propose a high-speed table tennis spin measurement system using single-frame multi-exposures based on M-sequence patterns and symmetric dot markers. We have experimentally evaluated that the system has sufficient measurement accuracy and a computation speed of more than 500 fps for simulated images with various spin amounts, axes, and viewing angles. …”
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Combining widely targeted metabolomics and RNA-sequencing to reveal the function analysis of Phyllanthus emblica Linn. Juice-induced poultry macrophages by Chenggang Liu, Jin jin, Binyi Sun

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The utilization of microbial cell-free DNA next-generation sequencing for the detection of human herpesvirus-8 in a quaternary care center by Rebecca Berger, Jennifer Jacobe, Fernando H. Centeno, Todd Lasco, Mayar Al Mohajer

“…While violaceous skin lesions characterize cutaneous Kaposi sarcoma, extracutaneous HHV8 is challenging to diagnose due to nonspecific symptoms. Objectives: We evaluated the role of microbial cell-free DNA next-generation sequencing (mcfDNA NGS) in diagnosing HHV8-related illness. …”
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Backhand-View-Based Continuous-Signed-Letter Recognition Using a Rewound Video Sequence and the Previous Signed-Letter Information by Ponlawat Chophuk, Kosin Chamnongthai

“…To evaluate performance of the proposed method, experiments with 10 participants were performed 20 times each, and the results revealed 96.07&#x0025; accuracy approximately which were improved significantly from the conventional methods using forehand and backhand.…”
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Genetic Diversity of Cowpea (Vigna unguiculata (L.) Walp.) Accession in Kenya Gene Bank Based on Simple Sequence Repeat Markers by Emily N. Wamalwa, John Muoma, Clabe Wekesa

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Surrogate-Assisted Multi-Objective Optimization for Simultaneous Three-Dimensional Packing and Motion Planning Problems Using the Sequence-Triple Representation by Ziang Liu, Tomoya Kawabe, Tatsushi Nishi, Shun Ito, Tomofumi Fujiwara

“…This framework comprises three key components: solution encoding, surrogate modeling, and evolutionary computation. The sequence-triple representation encodes complex packing solutions by a sequence of integers. …”
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Sequence signatures and mRNA concentration can explain two‐thirds of protein abundance variation in a human cell line by Christine Vogel, Raquel de Sousa Abreu, Daijin Ko, Shu‐Yun Le, Bruce A Shapiro, Suzanne C Burns, Devraj Sandhu, Daniel R Boutz, Edward M Marcotte, Luiz O Penalva

“…Using measurements of absolute protein and mRNA abundances in cellular lysate from the human Daoy medulloblastoma cell line, we quantitatively evaluate the impact of mRNA concentration and sequence features implicated in translation and protein degradation on protein expression. …”
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Consolidate knowledge or build scientific models? The role of online information-searching strategies in students’ prompt sequences with Generative Artificial Intelligence by Chiu-Lin Lai

“…Researchers have noticed that students’ ability to search, select, and evaluate information (e.g., their online information-searching strategies) may affect the quality of their learning and discussion with GAI. …”
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Lncident: A Tool for Rapid Identification of Long Noncoding RNAs Utilizing Sequence Intrinsic Composition and Open Reading Frame Information by Siyu Han, Yanchun Liang, Ying Li, Wei Du

“…The 10-fold cross-validation and ROC curve are used to evaluate the performance of Lncident. The main advantage of Lncident is high speed without the loss of accuracy. …”
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Genotyping-by-sequencing reveals low genetic diversity and peripheral isolation in Southern populations of Sophora koreensis, a Korean endemic shrub by Young-Ho Ha, Jong-Bin An, Jaesang Chung, Jung-Won Yoon, Hee-Young Gil

“…Abstract We evaluated the patterns of genetic diversity, structure, and differentiation both within and among populations levels of Sophora koreensis in South Korean populations using the genotyping-by-sequencing method. …”
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Single-cell sequencing combined with bulk RNA seq reveals the roles of natural killer cell in prognosis and immunotherapy of hepatocellular carcinoma by Jiahao Wu, Fan Yang, Guanqun Huang

“…WGCNA and single-cell sequencing RNA were performed to identify NK cell related genes. …”
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De novo identification of satellite DNAs in the sequenced genomes of Drosophila virilis and D. americana using the RepeatExplorer and TAREAN pipelines. by Bráulio S M L Silva, Pedro Heringer, Guilherme B Dias, Marta Svartman, Gustavo C S Kuhn

“…We combined the computational approach with data from the literature and chromosome mapping to obtain an overview of the major tandem repeat sequences of these species. The fact that all of the abundant tandem repeats (TRs) we detected were previously identified in the literature allowed us to evaluate the efficiency of TAREAN in correctly identifying true satDNAs. …”
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High Throughput Sequencing Technologies as a New Toolbox for Deep Analysis, Characterization and Potentially Authentication of Protection Designation of Origin Cheeses? by Elena Kamilari, Marios Tomazou, Athos Antoniades, Dimitrios Tsaltas

“…Additionally, they may assist the cheese-makers to better evaluate the quality, as well as the safety of their products. …”
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