Association Between Single-Nucleotide Polymorphisms in <i>Toll-like Receptor 3</i> (<i>tlr3</i>), <i>tlr7</i>, <i>tlr8</i> and <i>tirap</i> Genes with Severe Symptoms in Children P... by Adriana Souza Andrade, Aline Almeida Bentes, Lilian Martins Diniz, Silvia Hees Carvalho, Erna Geessien Kroon, Marco Antonio Campos

“…To identify the SNPs, PCR and sequencing were performed. The frequencies of the SNPs obtained were not discrepant when compared to the frequencies described in the Global ALFA, Global 1000 Genomes, Global gnomAD, American 1000 Genomes, and American gnomAD databases, except for the SNP in TLR7. …”
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Dissecting PTPN7‐driven aggressiveness in IDH‐wildtype astrocytomas: multi‐omics, clinical validation, and spatial transcriptomics for prognostic insights by Tung Liu, Yu-Chieh Lin, Pei-Chi Chang, Dueng-Yuan Hueng, Yao-Feng Li

“…We also applied CIBERSORT to evaluate the relationships between PTPN7 expression and 12‐principal cell states and 22 immune populations. …”
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Association of Kidd blood group distribution and genotypes specificity with the risk of coronary heart disease by LI Fei, QIU Jin, LI Huijun, MA Xiaojie, ZHAO Tiesuo, CHEN Wei

“…Correlation analysis and logistic regression were used to evaluate the influence of Kidd blood group antigen expression on coronary heart disease risk. …”
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Characterization of <i>Enterobacter cloacae</i> and <i>Citrobacter freundii</i> Species Complex Isolates with Decreased Susceptibility to Cephalosporins from United States Hospital... by Helio S. Sader, Timothy B. Doyle, John H. Kimbrough, Rodrigo E. Mendes, Mariana Castanheira

“…Background: <i>Citrobacter freundii</i> (CFC) and <i>Enterobacter cloacae</i> (ECLC) species complexes represent important causes of hospital-associated infections, frequently are related to outbreaks, and have a great ability to develop antimicrobial resistance. We evaluated a large collection of CFC and ECLC isolates with decreased susceptibility to broad-spectrum cephalosporins (Ceph-DS) from United States (US) hospitals. …”
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Construction of a Bacillus subtilis-based expression system for Eimeria acervulina profilin by Alfredo Panebra, Youngsub Lee, Hyun S. Lillehoj

“…Following three rounds of screening using 3-1E-specific monoclonal antibodies (mAbs), 25 higher expressor recombinants were isolated and sequenced to identify the signal peptide driving 3-1E expression. …”
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Presence of tumor DNA in aqueous humor is correlated with high risk uveal melanoma by Nicole Barwinski, Mael Lever, Philipp Rating, Leyla Jabbarli, Miltiadis Fiorentzis, Nikolaos E. Bechrakis, Dietmar R. Lohmann, Michael Zeschnigk, Claudia H. D. Le Guin

“…Tumor DNA was detected in at least one ocular fluid (AH or VB) in 43 of 88 evaluable patients (49%), with variant allele fractions (VAFs) ranging from 0.3 to 50%. …”
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Composition and predicted functions of dairy cattle's endometrial and rumen microbiome against the background of chronic endometritis by Elena A. Yildirim, Valentina A. Filippova, Larisa A. Ilina, Georgy Yu. Laptev, Andrey V. Dubrovin, Vasily A. Zaikin, Ksenia A. Kalitkina, Elena A. Korochkina, Irina A. Klyuchnikova, Ekaterina S. Ponomareva, Evgeny A. Brazhnik, Tatyana S. Smetannikova, Daria G. Tyurina

“…Assess study purposes was to evaluate changes in the composition and predicted metabolic pathways of the endometrial and rumen microbiota of cattle with chronic endometritis. …”
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Lactococcus petauri LZys1 modulates gut microbiota, diminishes ileal FXR-FGF15 signaling, and regulates hepatic function by Ouyang Li, Yingshun Zhou, Dayoung Kim, Han Xu, Zhijun Bao, Fan Yang

“…Subsequently, we analyzed changes in gut microbiota composition using 16S rRNA sequencing and quantified alterations in hepatic-intestinal bile acid (BA) profile. …”
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Predictive study of machine learning combined with serum Neuregulin 4 levels for hyperthyroidism in type II diabetes mellitus by Huilan Gu, Ye Lu

“…Additionally, a CNN+LSTM network was employed to extract spatial (thyroid morphology) and temporal (hemodynamics) features from ultrasound sequences. These features were then fused with biochemical indicators, such as NRG4, to develop the final SVM-CNN+LSTM multimodal predictive model.ResultsSerum NRG4 levels in T2DM+FT patients were significantly higher than those in the healthy Ctrl group (4.44 ± 1.25 vs. 2.17 ± 0.48 μg/L, P&lt; 0.05). …”
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The synergistic interaction effect between biochar and plant growth-promoting rhizobacteria on beneficial microbial communities in soil by Qianmei Zou, Longyuan Zhao, Lirong Guan, Ping Chen, Jie Zhao, Yueying Zhao, Yunlong Du, Yunlong Du, Yong Xie, Yong Xie

“…Specifically, high-throughput sequencing probed beneficial soil microbial communities and investigated the impact of BC and PGPR on cabbage yield and soil properties. …”
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Expanding Multilingual Co-Speech Interaction: The Impact of Enhanced Gesture Units in Text-to-Gesture Synthesis for Digital Humans by Ghazanfar Ali, Woojoo Kim, Muhammad Shahid Anwar, Jae-In Hwang, Ahyoung Choi

“…A comprehensive user study evaluated our hypotheses. The results underscored the positive impact of diverse gestures, supporting the first hypothesis. …”
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KRAS amplification in colorectal cancer: correlations with clinicopathological features and prognosis in patients and prediction of response to targeted therapy by Xuanhua Yang, Shiwei Feng, Qi Zhang, Wenxin Li, Shujuan Li, Min Zhi, Ping Lan, Sijing Cheng

“…KRAS mutational status was determined as wild-type (WT) or mutant (MUT) using qPCR and Sanger sequencing. CD8⁺ T lymphocyte infiltration was evaluated by immunohistochemistry in resected CRC specimens. …”
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Danshenol B alleviates central post-stroke pain by regulating the PIK3CG/NLRP3 signaling pathway by Panyang Li, Linna Yu, Jinzhong Yao, Tingting Zhao, Sen Zhao

“…Danshenol B was then administered (5, 10, 50 mg/kg) to CPSP mice to evaluate its analgesic effects, and thalamic PIK3CG and NLRP3 protein levels were measured to assess pathway involvement. …”
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Antimicrobial susceptibility of enterobacterales causing bloodstream infection in United States medical centres: comparison of aztreonam-avibactam with beta-lactams active against... by Helio S. Sader, John H. Kimbrough, Rodrigo E. Mendes, Mariana Castanheira

“…Carbapenem-resistant Enterobacterales (CRE) isolates were tested for β-lactamase–encoding genes using Next-generation sequencing. Results Aztreonam-avibactam was highly active against Enterobacterales; only 2 isolates showed aztreonam-avibactam MICs > 8 mg/L: 1 meropenem-susceptible E. coli and 1 K. aerogenes (CRE). …”
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Unravelling the pathogenic role and genotype-phenotype correlation of the USH2A p.(Cys759Phe) variant among Spanish families. by Raquel Pérez-Carro, Fiona Blanco-Kelly, Lilián Galbis-Martínez, Gema García-García, Elena Aller, Blanca García-Sandoval, Pablo Mínguez, Marta Corton, Ignacio Mahíllo-Fernández, Inmaculada Martín-Mérida, Almudena Avila-Fernández, José M Millán, Carmen Ayuso

“…Genetic analyses were performed by using classical molecular and Next-Generation Sequencing approaches. Probands of 57 unrelated families were molecularly studied and 63 patients belonging to these families were phenotypically evaluated.…”
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Screening and analysis of programmed cell death related genes and targeted drugs in sepsis by Juanjuan Song, Kairui Ren, Yi Wang, Dexin Zhang, Lin Sun, Zhiqiang Tang, Lili Zhang, Ying Deng

“…Diagnostic genes were selected using Least Absolute Shrinkage and Selection Operator (LASSO) and Random Forest (RF) methodologies. Single-cell RNA sequencing was utilized to assess gene expression in blood cells, while CIBERSORT was employed to evaluate immune cell infiltration. …”
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Neuro-leishmaniasis with cauda equina syndrome and cranial nerve palsy: a rare manifestation of recurrent atypical visceral leishmaniasis by Karthick Kumar Vaitheeswaran, Baidhnath Kumar Gupta, Rahul Krishnan G, Manish Soneja, Naval K. Vikram, Upendra Baitha, Amandeep Singh, Naveet Wig, Mudsser Azam, Ruchi Singh, Ajay Garg, Nishikant Damle, Yamini Dharmashaktu

“…Clinical and radiological evaluations suggested cauda equina syndrome and cranial nerve palsy, accompanied by generalized lymphadenopathy, subcutaneous nodules, and skin papules. …”
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Identifying pyroptosis-related prognostic genes in the co-occurrence of lung adenocarcinoma and COPD via bioinformatics analysis by Chaofan Cao, Zhaoshuang Zhong, Bo Wu, Yang Yang, Lingfei Kong, Shuyue Xia, Guixian Xiao

“…However, the underlying mechanisms of their coexistence are still not fully understood. Thus, this study evaluated the possible mechanisms and biomarkers of COPD and LUAD by analyzing public RNA sequencing databases via bioinformatics analysis. …”
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Prevalence and molecular characterization of drug-resistant Mycobacterium tuberculosis in Heyuan City in China by Dan Qiu, Dan Qiu, JianHong Ma, ZhiFang Liu, XiangXing Zeng

“…Eight genes had 127 altered nucleotide sequences, with KatG315 (20.47%) having the most significant incidence of mutations. …”
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In Vitro Characterization and Safety Assessment of <i>Streptococcus salivarius</i>, <i>Levilactobacillus brevis</i> and <i>Pediococcus pentosaceus</i> Isolated from the Small Intes... by Nwabisa Happiness Kokwe, Freedom Tshabuse, Feroz Mahomed Swalaha

“…These strains were further evaluated for probiotic properties such as transit resistance to simulated upper gastrointestinal conditions, antagonist activity, haemolytic activity, and cell surface properties such as autoaggregation, co-aggregation and hydrophobicity, <i>in vitro</i>. …”
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