Succinylation-related molecular activities in cancer: metabolic adaptations, immune landscape, and prognostic significance in colorectal cancer by Zhihang Jiang, Zhihang Jiang, Xiaoqing Li, Xiaoqing Li, Long Hu, Zheng Jiang

“…PCED1A protein expression was evaluated by immunohistochemistry and Western blotting. …”
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Genetic engineering of E. coli K-12 for heterologous carbohydrate antigen production by Caixia Li, Hongxu Zha, Ziyan Jiao, Keyan Wei, Huaiyu Gao, Feiyi Lai, Zuoyong Zhou, Hongyan Luo, Pei Li

“…The scarless chromosomal insertions were confirmed by whole-genome sequencing and the polysaccharide phenotypes of all glycoengineered E. coli mutants were evaluated through growth curves, silver staining, western blot, and flow cytometry. …”
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Outcomes Following Observation of Small Non-Functioning Neuroendocrine Tumors of the Pancreas. Data from the Registry of the Loginov Moscow Clinical Scientific Center by I. E. Khatkov, D. A. Salimgereeva, I. Yu. Feidorov, A. A. Konyakhina, A. L. Petrova

“…Aim: to evaluate results of follow-up of patients with pancreatic non-functioning neuroendocrine tumors of the stage T1–T2 using a medical registry.Materials and methods. …”
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Association between triglycerides and remnant cholesterol levels and spine bone mineral density in Duchenne muscular dystrophy by Yayu Wang, Yue Chang, Pei Zhang, Zhuoyin Zheng, Xiuyi Ai, Shu Zhang, Shiwen Wu

“…Clinical data included lipid profiles (triglycerides [TGs], remnant cholesterol [RC]); bone mineral density of the lumbar spine was evaluated using Dual-energy X-ray absorptiometry (DXA); and corticosteroid use, including treatment status, dose, and duration. …”
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Deoxycholic acid induces gastric intestinal metaplasia by activating STAT3 signaling and disturbing gastric bile acids metabolism and microbiota by Duochen Jin, Keting Huang, Miao Xu, Hongjin Hua, Feng Ye, Jin Yan, Guoxin Zhang, Yun Wang

“…However, the mechanisms underlying how DCA facilitates IM in the gastric epithelium need exploration. We evaluated IM and bile reflux in corpus tissues from 161 subjects undergoing GC screening. …”
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Advancing personalized, predictive, and preventive medicine in bladder cancer: a multi-omics and machine learning approach for novel prognostic modeling, immune profiling, and ther... by Han Yan, Xinyu Ji, Bohan Li

“…Survival analysis, immune infiltration, pathway enrichment, and drug sensitivity were evaluated to validate the model.ResultsThe ICDRS, based on eight key genes (IL32, AHNAK, ANXA5, FN1, GSN, CNN3, FXYD3, CTSS), effectively stratified BLCA patients into high- and low-risk groups with significant differences in overall survival (OS, P < 0.001). …”
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Bacterial biodiversity and optimization of pilot plant-based storage parameters of beet thick juice under Egyptian environmental conditions by Marwa Abdelhak, Osama Abdel-Hafeez Mohamed Al-Bedak, Mahmoud N. Abdelmoez, Adel Ahmed Abdellah, El-Sayed A. Abdel-Rahman, Mohamed M. Abd El-Wahab

“…The relationships between bacterial count (CFUs), pH, lactic acid (LA) concentration, and reducing sugars (RS) content were evaluated. CFUs increased gradually with the length of the storage period in all tanks, reaching its peak (350 CFU/mL) in the control tank at 35 °C. …”
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Saikosaponin A Mediates the Anti-Acute Myeloid Leukemia Effect via the P-JNK Signaling Pathway Induced by Endoplasmic Reticulum Stress by Sun XH, Chai YH, Bai XT, Li HX, Yang PP, Xi YM

“…RNA sequencing was performed to identify differentially expressed genes and enriched signaling pathways. …”
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Effects of oral gavage with periodontal pathogens and plaque biofilm on gut microbiota ecology and intestinal tissue architecture in mice: a mechanistic study by Lan Huang, Song Ge, Kun Yang, Lian Duan, Li Gao, Yu Zhen Li, Yu Shi Yi

“…ObjectiveThis study aimed to establish an in vitro model simulating periodontal biofilm architecture with three representative periodontal pathogens and evaluate its systemic impact through oral gavage administration in C57BL/6 mice. …”
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CircRNA hsa_circ_0004781 promoted cell proliferation by acting as a sponge for miR-9-5p and miR-338-3p and upregulating KLF5 and ADAM17 expression in pancreatic ductal adenocarcino... by Kun-Lin Lee, Jun-Jen Liu, Wei-Jan Huang, Ching-Sheng Hung, Yu-Chih Liang

“…The biological functions of hsa_circ_0004781 were evaluated using loss-of-function and gain-of-function approaches with circRNA expression plasmids and real hsa_circ_0004781. …”
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Relationship between gut microbiota and vascular calcification in hemodialysis patients by Wen-Han Bao, Wen-Ling Yang, Chun-Yan Su, Xin-Hong Lu, Lian He, Ai-Hua Zhang

“…According to the abdominal aortic calcification (AAC) scores, the participants were divided into the high AAC score group and the low AAC score group. High-throughput sequencing of the gut microbiota was performed and the results were evaluated by alpha diversity, beta diversity, species correlation, and model predictive analyses.Results The prevalence of VC was 54.79% (40/73) in the study. …”
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Comparative gene expression analysis of Beauveria bassiana against Spodoptera frugiperda by Hamdy H. Aly, Yun Meng, Dun Wang

“…Our objectives were to analyze the B. bassiana gene expression variation during insect infection compared to grow on artificial media and to evaluate the effects of different spore concentrations on larval mortality, development, and reproduction. …”
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Integrated bioinformatics and experimental analysis of mitochondrial-associated membrane function and mechanism in acute respiratory distress syndrome​​ by Yanqiong Zhou, Qiuying Chen, Xiaoxia Wang, Kaimin Lv, Hui Huang, Jifeng Feng, Bijun Luo

“…Publicly available RNA-sequencing data from ARDS and control samples were analyzed to identify differentially expressed genes (DEGs). …”
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Development of PowerMag System II for Isolation of Circulating Tumor Cells with Improved Purity by Cheng-Rou Ho, Hui-Ju Tsai, Jin-Ru Wang, Chia-Te Wang, Chiuan-Chian Chiou, Ju-Chien Cheng, Sum-Fu Chiang, Ching-Ping Tseng

“…The improved performance of PM II allowed for direct profiling of cancer-related gene mutations by next-generation sequencing without cell picking and further purification. …”
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Intestinal Microbiota Dysbiosis Disrupts the Mucosal Barrier, Triggering Inflammatory Responses in Gut-Kidney Interaction and Exacerbating Diarrhea by Shen J, Fang L, Wu Y, Deng N, Peng X, Li D, Tan Z

“…Measure the levels of NOD-like receptor family pyrin domain containing protein 3 (NLRP3) inflammasome-related molecules and inflammatory factors in the colon and kidney tissues. 16S rRNA sequencing combined with bioinformatics analysis was employed to evaluate the diversity and species composition of the intestinal microbiota, conduct correlation analysis, and predict its metabolic functions.Results: The model mice exhibited increased fecal water content and decreased body temperature. …”
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Liensinine reshapes the immune microenvironment and enhances immunotherapy by reprogramming metabolism through the AMPK-HIF-1α axis in hepatocellular carcinoma by Jiahao Liu, Xuan Zhang, Xiaofei Fan, Peng Liu, Ze Mi, Hongpei Tan, Pengfei Rong

“…Metabolic reprogramming was analyzed through RNA sequencing, Seahorse metabolic assays, and glucose/lactate measurements. …”
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Carboplatin-resistance-related DNA damage repair prognostic gene signature and its association with immune infiltration in breast cancer by Shuwen Dong, Anqi Li, Ruixin Pan, Jin Hong, Zheng Wang, Kunwei Shen

“…Additionally, drug sensitivity predictions were made to evaluate the efficacy of other DNA damage-related drugs across different risk groups. …”
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Exploration and Prospects of DeepSeek Applications in Engineering Hydrology by GAO Zi-xuan, SONG Xin-yi

“…In the data analysis phase, the annual maximum water levels, 3-day and 5-day moving average maximum sequences during the flood season were generated, and the Pearson Type III (P-III) distribution was used to calculate key frequency design values such as 1% and 5%. …”
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Staphylococcus aureus vesicles impair cutaneous wound healing through p38 MAPK-MerTK cleavage-mediated inhibition of macrophage efferocytosis by Jiaxin Ou, Kangxin Li, Hui Yuan, Shaohua Du, Tingting Wang, Qiannan Deng, Huimei Wu, Weiyan Zeng, Kui Cheng, Kutty Selva Nandakumar

“…Hematoxylin and eosin (H&E) staining and wound size measurements were used to evaluate the wound healing process. Phosphorylation of signaling pathways was detected by WB, and efferocytosis receptor expression was measured using RNA sequencing, qPCR, and flow cytometry. siRNA and pathway inhibitors were used to investigate the roles of key receptors and signaling pathways in efferocytosis. …”
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EcoTaskSched: a hybrid machine learning approach for energy-efficient task scheduling in IoT-based fog-cloud environments by Asfandyar Khan, Faizan Ullah, Dilawar Shah, Muhammad Haris Khan, Shujaat Ali, Muhammad Tahir

“…Before preprocessing the data was normalized, treated with feature engineering and augmentation, and then split into training and test sets. To evaluate performance, the proposed EcoTaskSched model demonstrated superiority by significantly reducing energy consumption and improving job completion rates compared to baseline models. …”
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