Bacterial Community in Sugarcane Rhizosphere Under <i>Bacillus subtilis</i> Inoculation and Straw Return by Francisco de Alcântara Neto, Danielly Araújo Pinheiro, Sandra Mara Barbosa Rocha, Marcos Renan Lima Leite, Romário Martins Costa, Janderson Moura da Silva, Sabrina Hermelindo Ventura, Mayanna Karlla Lima Costa, Thâmara Kelly dos Santos Apollo Sousa, Rafael de Souza Miranda, Karolayne Ribeiro Caetano, Erika Valente de Medeiros, Arthur Prudêncio de Araujo Pereira, Lucas William Mendes, Ademir Sérgio Ferreira Araujo

“…Three treatments were evaluated: (I) bulk soil (bulk), (II) rhizosphere soil with straw return but no <i>B. subtilis</i> inoculation (straw), and (III) rhizosphere soil with straw return and <i>B. subtilis</i> inoculation (straw + Bacillus). …”
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Analysis of epilepsy‐associated variants in HCN3 – Functional implications and clinical observations by Peiwei Zhao, Hongbo Xiong, Gunagtao Kuang, Chen Sun, Xiankai Zhang, Yufeng Huang, Sukun Luo, Lei Zhang, Jun Jiang, Xuelian He

“…Methods We recruited a cohort of 298 epilepsy patients to screen for genetic variants in the HCN3 (NM_020897.3) using Sanger sequencing. We identified rare variants and conducted functional assays to evaluate their pathogenicity. …”
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Human iPSC-derived pericyte-like cells carrying APP Swedish mutation overproduce beta-amyloid and induce cerebral amyloid angiopathy-like changes by Ying-Chieh Wu, Šárka Lehtonen, Kalevi Trontti, Riitta Kauppinen, Pinja Kettunen, Ville Leinonen, Markku Laakso, Johanna Kuusisto, Mikko Hiltunen, Iiris Hovatta, Kristine Freude, Hiramani Dhungana, Jari Koistinaho, Taisia Rolova

“…Additionally, bulk RNA sequencing was utilized to compare the transcriptional profiles between the two groups. …”
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Phenotypic and oncological insights in ANNA1 autoimmunity: Age stratification and biomarker analysis by Naveen Kumar Paramasivan, Majed Masoud, Carley Karsten, Anza Zahid, Haidara Kherbek, Anastasia Zekeridou, Sri Raghav Sista, Surendra Dasari, Andrew M. Knight, Georgios Mangioris, John R. Mills, Andrew McKeon, Sean J. Pittock, Divyanshu Dubey

“…Phage immunoprecipitation sequencing (PhIP‐Seq) and neurofilament light chain (NfL) measurements were done in patient sera/CSF with archived samples. …”
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Enhance the therapeutic efficacy of human umbilical cord-derived mesenchymal stem cells in prevention of acute graft-versus-host disease through CRISPLD2 modulation by Qing Xu, Rui Wang, Ke Sui, Yuxi Xu, Ya Zhou, Yuxuan He, Ziyi Hu, Qi Wang, Xiaodong Xie, Xiaoqi Wang, Shijie Yang, Lingyu Zeng, Jiang F. Zhong, Zheng Wang, Qingxiao Song, Xi Zhang

“…The downstream regulatory effects of CRISPLD2 overexpression were assessed through bulk RNA sequencing. Additionally, we evaluated its impact on cellular senescence using Western blotting and β-galactosidase (SA-β-gal) staining. …”
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Construction and Validation of A Prognostic Model for Lung Adenocarcinoma 
Based on Ferroptosis-related Genes by Zhanrui ZHANG, Wenhao ZHAO, Zixuan HU, Chen DING, Hua HUANG, Guowei LIANG, Hongyu LIU, Jun CHEN

“…The expression of ferroptosis-related genes in tumor tissues can be used to predict patients' future survival times, aiding doctors and patients in anticipating disease progression. Based on the sequencing data of lung adenocarcinoma (LUAD) patients from The Cancer Genome Atlas (TCGA) database, this study identified genes involved in the regulation of ferroptosis, constructed a prognostic model, and evaluated the predictive performance of the model. …”
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OTUD1 exacerbates sepsis-associated encephalopathy by promoting HK2 mitochondrial release to drive microglia pyroptosis by Guoqing Jing, Hailong Gong, Han Wang, Jing Zuo, Die Wu, Huifan Liu, Xing Wang, Min Yuan, Yun Xia, Tongtong Du, Wanhong Liu, Xiaojing Wu, Xuemin Song

“…Neuronal and synaptic damage were evaluated using HE and Nissl staining, as well as transmission electron microscopy. …”
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Oestrogen suppresses the adipogenesis of fibro/adipogenic progenitors through reactivating the METTL3–ESR1‐mediated loop in post‐menopausal females by Hao Zhou, Shujing Feng, Jinkui Cai, Xiexiang Shao, Siyuan Zhu, Han Zhou, Yongmin Cao, Ru Wang, Xingzuan Lin, Jianhua Wang

“…The effects of estrogen supplementation on muscular fatty infiltration and locomotor function in OVX mice were evaluated by immunofluorescent staining and functional analysis. …”
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The dysbiosis of gut microbiota and dysregulation of metabolites in IgA nephropathy and membranous nephropathy by Lei Zhang, Lan Hu, Lan Hu, Lan Hu, Li Tan, Zhenjie Zhang, Mengying Chen, Wenbo Gan, Li Chen, Yan Zou, Shi Wang, Yu Pang, Zhenxin Fan, Junjie Liu

“…Microbial diversity and composition were assessed using metagenomic analysis, while metabolic profiles were evaluated through untargeted LC -MS-based metabolomics. …”
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Diagnostic potential of miRNA-135A1 in human papillomavirus associated cervical lesions by D. S. Elkin, M. I. Taubinskaya, N. V. Elkina, R. S. Faskhutdinov, M. D. Fedorova, A. N. Katargin, K. I. Zhordania, E. A. Mustafina, E. A. Grivachev, L. S. Pavlova, S. V. Vinokurova

“…MiRNA expression in clinical samples was assessed by both next generation sequencing and quantitative stem-loop polymerase chain reaction (sl-qPCR). …”
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Human neural stem cell-derived exosomes activate PINK1/Parkin pathway to protect against oxidative stress-induced neuronal injury in ischemic stroke by Mengke Zhao, Jiayi Wang, Shuaiyu Zhu, Shensen Zhang, Chao Han, Chengcheng Tan, Yubing Huang, Zhaokai Sun, Liang Wang, Jing Liu

“…Subsequently, the protective effects of hNSC-Exos on MCAO rats were evaluated, including histological studies, behavioral assessments. …”
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Utilizing bioinformatics to identify biomarkers and analyze their expression in relation to immune cell ratios in femoral head necrosis by Dongchen Li, Dongchen Li, Zhilong Huang, Teng Ma, Yu Su, Yu Su, Zhao Li, Zhao Li, Liang Sun, Ming Li, Zhong Li, Yao Li, Qian Wang, Yao Lu

“…Hub genes were identified using the CytoHubba plugin, followed by enrichment analysis, and their expression levels were evaluated using the ROC curve. In addition, we performed expression data visualization and ROC curve analysis on the external dataset GSE74089 to further evaluate the discriminative power of the hub genes. …”
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A Novel Research Paradigm for Sarcopenia of Limb Muscles: Lessons From the Perpetually Working Diaphragm's Anti‐Aging Mechanisms by Enhui Li, Rui Wang, Yanli Li, Xiang Zan, Shufen Wu, Yiru Yin, Xiaorong Yang, Litian Yin, Yu Zhang, Jianguo Li, Xin Zhao, Ce Zhang

“…Histological assessments evaluated muscle mass in both the DIA and tibialis anterior (TA). …”
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TRAP1 Improves Diabetic Retinopathy by Preserving Mitochondrial Function by Li Y, Xu W, Zhao G, Guo Y, Wang L, Du Q, Fei Y, Hu X, Hu H, Chen L, Xu Y

“…The role of TRAP1 in ferroptosis in ARPE-19 cells was evaluated with or without ferrostatin-1 (Fer-1) and erastin. …”
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Acid-sensing ion channel-1 contributes to the failure of myelin sheath regeneration following spinal cord injury by transcellular delivery of PGE2 by Zuomeng Wu, Tianyu Han, Yixiang Dong, Wang Ying, Huang Fang, Yunlei Liu, Peiwen Song, Cailiang Shen

“…The concentration of prostaglandin E2 (PGE2) within NSC-derived extracellular vesicles were evaluated by ELISA. Small-interfering RNAs (siRNAs) were used to knock down Asic1a and Ptgs2 expression in NSCs. …”
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CircRNA_1809 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells through miR-370-3p by Lei Zhang, Yidan Xu, Ningning Liu, Mengjie Xu, Refeina Tuerxun, Mireyeti Shahatibieke, Yuan Yao

“…In a previous study by our team, all the differentially expressed circRNAs and messenger RNAs (mRNAs) involved in the osteogenic differentiation of BMMSCs were identified via high-throughput sequencing, and a competing endogenous RNA (ceRNA) regulatory network was constructed via bioinformatics analysis. …”
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Genome-wide association study of Northern corn leaf blight (NCLB) resistance using temperate and subtropical maize recombinant inbred lines by Xiaodong Gong, Fuyan Jiang, Ranjan K. Shaw, Jiachen Sun, Tao Dai, Yaqi Bi, Yanhui Pan, Jiao Kong, Haiyang Zong, Xiaoping Yang, Babar Ijaz, Xingming Fan

“…Conclusions This study identifies two candidate genes associated with NCLB resistance through a genome-wide association analysis using 529 recombinant inbred lines evaluated across subtropical and tropical environments. …”
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Imaging Microstructural Parameters of Breast Tumor in Patient Using Time-Dependent Diffusion: A Feasibility Study by Shuyi Peng, Peng Sun, Jie Liu, Juan Tao, Wenying Zhu, Fan Yang

“…Diffusion MRI acquisition was conducted with a combination of pulsed gradient spin-echo sequences (PGSE) and oscillating gradient spin-echo (OGSE) on a 3T MRI scanner. …”
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Comparative efficacy of preventive vs. therapeutic resveratrol in modulating gut microbiota and alleviating inflammation in DSS-induced colitis by Senmei Qin, Zongjing Yang, Jinqing Lei, Qingli Xie, Linsui Jiang, Yuanyuan Fan, Yonggu Luo, Kecong Wei, Wei Luo, Bing Yu

“…Clinical outcomes, intestinal barrier integrity, inflammatory cytokines, macrophage polarization, TLR4/NF-κB signaling, and gut microbiota (16S rRNA sequencing) were systematically evaluated. Results Preventive RSV (PRE) outperformed therapeutic RSV across all metrics. …”
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Prospective study on diagnostic efficacy of targeted NGS vs. traditional testing for respiratory infections in myelosuppressed hematology patients by Chen Li, Jing Wu, Yuhu Feng

“…This study aimed to evaluate the diagnostic efficacy of targeted next-generation sequencing (tNGS) compared to traditional microbiological testing methods (TMT) in detecting respiratory infections among myelosuppressed hematological patients.MethodsThis prospective study included 20 patients aged ≥15 years with myelosuppressed hematological disease and respiratory infection, admitted to the hematology department of Fuyang People’s Hospital between January and May 2024. …”
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