Heme oxygenase 1 confers gilteritinib resistance in FLT3-ITD acute myeloid leukemia in a STAT6-dependent manner by Tianzhuo Zhang, Danna Wei, Yun Zhan, Zhengmei Long, Tingting Lu, Peng Zhao, Rui Gao, Qian Kang, Luxin Zhang, Min Liu, Xueying Yang, Jishi Wang

“…We previously discovered that heme oxygenase 1 (HO1) is crucial for chemoresistance in AML, but the detailed molecular mechanism of that remains unclear. Methods RNA sequencing was conducted to assess transcriptomic changes in three pairs of AML cells after regulating the expression of HO1. …”
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Rosemary Extract Reduces Odor in Cats Through Nitrogen and Sulfur Metabolism by Gut Microbiota–Host Co-Modulation by Ziming Huang, Miao Li, Zhiqin He, Xiliang Yan, Yinbao Wu, Peiqiang Mu, Jun Jiang, Xu Wang, Yan Wang

“…The diverse bioactive compounds in plant extracts make them a promising candidate for effective odor reduction. This study evaluated twelve plant extracts for deodorizing efficacy via in vitro fermentation tests. …”
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Cistanche tubulosa glycosides ameliorate cognitive decline in APP/PS1 mice via modulation of gut microbiota and fatty acid metabolism: insights from multi-omics and experimental va... by Rui Hou, Wei Song, Yi Nan, Yiyi Gong, Jieying Liu, Jialin Liu

“…Aβ burden and inflammatory factors were evaluated by immunohistochemistry and ELISA. Gut microbiota was analyzed using 16S rRNA sequencing. …”
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Molecular Identification of <i>Anopheles</i> (Diptera: Culicidae) Species in Native Communities of a Northeastern Region of Peru by Eddyson Montalvo-Sabino, Marianella Villegas-Pingo, Jhon Zumaeta, Lizandro Gonzales, Rafael Tapia-Limonchi, Marta Moreno, Christian R. González, Stella M. Chenet

“…Only specimens morphologically identified as <i>Anopheles</i> sp. were subjected to molecular confirmation through PCR amplification and sequencing of the <i>Cox1</i> barcode region. <i>Plasmodium</i> parasites were detected using nested PCR targeting of the 18S rRNA subunit, while human blood meal feeding was evaluated using a human <i>β-globin</i> marker. …”
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Assessing Image Quality in Multiplexed Sensitivity-Encoding Diffusion-Weighted Imaging with Deep Learning-Based Reconstruction in Bladder MRI by Seung Ha Cha, Yeo Eun Han, Na Yeon Han, Min Ju Kim, Beom Jin Park, Ki Choon Sim, Deuk Jae Sung, Seulki Yoo, Patricia Lan, Arnaud Guidon

“…The weighted kappa test and intraclass correlation were used to evaluate the interobserver agreement in the qualitative and quantitative analyses, respectively. …”
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Bacterial-charged biochar enhances plant growth and mitigates microplastic toxicity by altering microbial communities and soil metabolism by Muhammad Afzal, Xiyu Tan, Yihang Ouyang, Yihang Chen, Qihua Liang, Mehmood Jan, Arif Ali Khattak, Xiaolin Wang, Xiaoyuan Chen, Xiaoying Zhang, Zhiyuan Tan

“…Using qPCR, high-throughput sequencing, and untargeted metabolomics, we examined gene expression, microbial diversity, and metabolomic profiles. …”
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Unleashing the potential of a low CpG Passer transposon for superior CAR-T cell therapy by Jianyao Zeng, Yan Sun, Yan Sun, Yuan Fang, Xiaodie Wang, Qian Huang, Pingjing Zhang, Meiqi Shao, Pei Wang, Jingbo Cheng, Meng Di, Tao Liu, Qijun Qian, Qijun Qian, Qijun Qian

“…Nonetheless, non-methylated CpG sequences within plasmid DNA can elicit an inflammatory response via Toll-like receptor 9 (TLR9) during CAR-T cell preparation, adversely affecting transgene expression. …”
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Personalized colorectal cancer risk assessment through explainable AI and Gut microbiome profiling by Pierfrancesco Novielli, Simone Baldi, Donato Romano, Michele Magarelli, Domenico Diacono, Pierpaolo Di Bitonto, Giulia Nannini, Leandro Di Gloria, Roberto Bellotti, Amedeo Amedei, Sabina Tangaro

“…The models were trained on 16S rRNA sequencing data from 453 patients and evaluated through cross-validation, achieving AU-ROC and AU-PRC scores of 0.71 and 0.67, respectively. …”
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Cyclooxygenase-1 deletion in 5 × FAD mice protects against microglia-induced neuroinflammation and mitigates cognitive impairment by Jie Wang, Hong Ni, Yu Wang, Luyao Wei, Hanqing Ding, Zhongzhao Guo, Hao Pan, Ying Yu, Jia Luo, Weidong Pan, Deheng Wang, Zun-Ji Ke

“…Methods We conducted backcrossing between COX-1 knockout (KO) and 5 × FAD mice to evaluate the effect of COX-1 deficiency on neuroinflammation. …”
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Unveiling the role of TGF-β signaling pathway in breast cancer prognosis and immunotherapy by Yifan Zheng, Yifan Zheng, Li Li, Wenqian Cai, Lin Li, Rongxin Zhang, Wenbin Huang, Wenbin Huang, Yulun Cao

“…We identified 54 genes associated with the TSP from the Molecular Signatures Database (MSigDB) and analyzed both data types to evaluate TSP activity. Using weighted gene co-expression network analysis (WGCNA), we identified modules linked to TSP activity. …”
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Cell‐free DNA for detection and monitoring of extramedullary AML relapse by Henri C. Hupe, Clara P. Wienecke, Stephan Bartels, Elisa Schipper, Jannika Leßmann, Alina Lasch, Maximilian Bader, Razif Gabdoulline, Martin Neugebohren, Elke Dammann, Hans H. Kreipe, Ulrich Lehmann, Anke K. Bergmann, Nataliya Di Donato, Michael Stadler, Matthias Eder, Arnold Ganser, Florian H. Heidel, Felicitas Thol, Michael Heuser

“…To date, measurable residual disease (MRD) assessment for this difficult‐to‐treat patient cohort has not been established. In this study, we evaluated highly sensitive next‐generation sequencing (NGS) of IEM‐AML tumor and compared it with cell‐free DNA (cfDNA) from plasma, as well as highly sensitive NGS analysis of bone marrow mononuclear cells (BMMC) and peripheral blood mononuclear cells (PBMC), in a cohort of 15 IEM‐AML patients with 19 IEM‐AML episodes. cfDNA demonstrated a superior representation of IEM‐AML tumor mutations compared to BMMC or PBMC, with a median variant allele frequency (VAF) of 0.8% and a mutation detection rate of 62% (37 of 60 mutations), compared to a median VAF of 0.05% and detection rate of 27%, respectively (16 of 60 mutations, p < 0.01). …”
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Pyrotinib targeted EGFR/GRP78 mediated cell apoptosis in high EGFR gene copy number gastric cancer by Lingbo Bao, Xudong Wang, Xiuyong Liao, Dong Li, ChunXue Li, Nan Dai, Xiaoyan Dai, Jing Yang, Nana Hu, Xueling Tong, Zhenjie He, Yuancheng Zhao, Zheng Liu, Yue Hu, Jinlu Shan, Dong Wang, Mengxia Li, Qian Chen

“…Methods Using EGFR-high copy number gastric cancer cell lines, primary cells and subcutaneous tumor models in nude mice, we systematically evaluated pyrotinib’s anti-tumor activity through viability assays, apoptosis analysis, and transcriptomic profiling. …”
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Impact of toothpaste use on the subgingival microbiome: a pilot randomized clinical trial by Margarita Iniesta, Viviane Vasconcelos, Florencia Laciar, Paula Matesanz, Mariano Sanz, David Herrera

“…Subgingival samples were collected at baseline and after 6 weeks and processed using high-throughput sequencing technology (Miseq®). Diversity metrics were calculated and the microbiome composition was analyzed using PERMANOVA, ANOSIM and PERMDISP. …”
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Assessment of CDKN2A homozygous and heterozygous deletions in gliomas across multiple detection platforms by Hainan Li, Ningning Luo, Chongzhu Fan, Tiantian Han, Xuemin Wei, Xing Zhang, Lijun Dai, Lei Ye, Dongsheng Chen, Linbo Cai, Zhi Li

“…In conclusion, our study utilized four platforms to comprehensively assess the status of CDKN2A in gliomas and evaluated the performance of each platform. We established cutoff values of FISH to confirm CDKN2A status. …”
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Insight into Antifungal Metabolites from <i>Bacillus stercoris</i> 92p Against Banana Cordana Leaf Spot Caused by <i>Neocordana musae</i> by Qunfang Yu, Pengbo He, Yanxiang Qi, Pengfei He, Ayesha Ahmed, Xin Zhang, He Zhang, Yixin Wu, Shahzad Munir, Yueqiu He

“…Here, we identified a new bacterial strain, <i>Bacillus stercoris</i> 92p, from the rhizosphere soil of banana. We evaluated its ability to suppress the growth of <i>N. musae</i> and other fungal pathogens that cause leaf spot disease in bananas. …”
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Shengyu decoction ameliorates knee osteoarthritis by inhibiting endoplasmic reticulum stress via Piezo1 channels by Xuyu Song, Ying Liu, Xianhui Shen, Lei Zhang, Hongwei Kong, Siyi Chen, Lisong Sheng, Lisong Sheng, Rong Sun, Rong Sun, Rong Sun

“…The active ingredients and mechanisms of SYD were analyzed through ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), RNA sequencing, molecular docking, and surface plasmon resonance (SPR). …”
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Genetic Structure and Optimal Population Size of Wild and Mass-Selected Silver Pomfret (<i>Pampus argenteus</i>) in China: The Implications for Conservation and Selection Breeding... by Mengya Xiao, Haipeng Yu, Yong Deng, Weixu Jiang, Yuanwen Zhang, Minglu Gao, Cheng Zhang, Jiabao Hu, Man Zhang, Shanliang Xu, Danli Wang, Yajun Wang

“…This study implemented a comprehensive molecular strategy to evaluate genetic parameters in wild populations along China’s coast and three successive selected generations. …”
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Updated overall survival data and predictive biomarkers of autologous NK cells plus Sintilimab as second-line treatment for advanced non-small cell lung cancer by Lin Jia, Naifei Chen, Xiao Chen, Chao Niu, Ziling Liu, Kewei Ma, Lei Yang, Yuguang Zhao, Wei Song, Jin Lu, Chen Chen, Xiaofeng Cong, Xu Wang, Yinghui Xu, Guozhen Cui, Zengguang Liu, Rongrong Chen, Huan Yin, Na Zhang, Jiuwei Cui

“…Multicolor immunofluorescence staining was applied to evaluate static markers within the tumor microenvironment. …”
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Cutaneous dysbiosis in girls with vulvar lichen sclerosus by Liying Sun, Huihui Gao, Hongyu Chen, Chenfei Ren, Junwen Zhang, Qiuxiang Shen, Li Zhu, Dandan Chen, Lingling Jin, Chaoqun Wang, Fengxia Li, Lan Yu

“…We performed a comparative analysis of 16S ribosomal RNA (rRNA) sequences from vulvar skin samples of 18 girls with VLS, 15 girls with labial adhesions, and 11 healthy girls. …”
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Molecular markers of reduced behavioral sensitivity to transfluthrin in Anopheles gambiae s.s. from Western Kenya by Stephen Okeyo, Dieunel Derilus, Lucy Mackenzie Impoinvil, Nsa Dada, Diana Omoke, Helga Saizonou, Cynthia Awuor Odhiambo, Nicola Mulder, Gerald Juma, Benard W. Kulohoma, John E. Gimnig, Luc S. Djogbénou, Audrey Lenhart, Eric Ochomo

“…Using a high-throughput screening system (HITSS), we evaluated 600 mosquitoes across three populations (Bungoma field population, the insecticide-resistant Pimperena lab strain, and the susceptible Kisumu lab strain), categorizing them as responders or non-responders based on their SR avoidance behavior. …”
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