Dynamic Changes in Rumen Microbial Diversity and Community Composition Within Rumen Fluid in Response to Various Storage Temperatures and Preservation Times by Chang Liu, Jin Cheng, Yunong Xie, Kehui Ouyang, Mingren Qu, Ke Pan, Qinghua Qiu

“…DNA was extracted at each time point for 16S rRNA gene sequencing to evaluate the rumen microbial diversity and community composition. …”
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Non-small cell lung cancer patients treated with Anti-PD1 immunotherapy show distinct microbial signatures and metabolic pathways according to progression-free survival and PD-L1 s... by David Dora, Balazs Ligeti, Tamas Kovacs, Peter Revisnyei, Gabriella Galffy, Edit Dulka, Dániel Krizsán, Regina Kalcsevszki, Zsolt Megyesfalvi, Balazs Dome, Glen J. Weiss, Zoltan Lohinai

“…Gut bacterial signatures were evaluated by metagenomic sequencing and correlated with progression-free survival (PFS), PD-L1 expression and other clinicopathological parameters. …”
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Study protocol for a stepped-wedge implementation study investigating the intersectoral collaboration of implementing the TREAT INTERACT intervention for primary school teachers an... by Ane-Marthe Solheim Skar, Ingunn Marie S. Engebretsen, Nora Braathu, Harriet Aber, Harald Bækkelund, Melf-Jakob Kühl, Marjorie Mukisa, Joyce Sserunjogi Nalugya, Norbert Skokauskas, Vilde Skylstad, Tore Wentzel-Larsen, TREAT research consortium, Juliet Ndimwibo Babirye

“…The main study utilize a stepped-wedged trial design with six starting sequences, randomizing three schools to the intervention at each interval, while the remaining act as “controls”. …”
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Biogenic synthesis of titanium nanoparticles by Streptomyces rubrolavendulae for sustainable management of Icerya aegyptiaca (Douglas) by Enayat M. Elqady, Eman El-said, Asmaa A. Tharwat, Lina A. Abou El-Khashab, Inas M. Y. Mostafa, Fatma Z. Hamed, Wesam M. Morsi, Mohamed M. Rezk, Inas M. Abou El-Enain

“…In all, seven actinobacterial isolates, the most potent of which has the potential to produce titanium hydroxide-based nanoparticles (TiO2-NP2), have DNA sequences that are 99.9% like those of Streptomyces rubrolavendulae (MCN2) according to nucleotide alignment and a phylogenetic tree. …”
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Dynamic Monitoring of Circulating Tumor DNA to Predict the Risk of Non In Situ Recurrence of Postoperative Glioma: A Prospective Cohort Study by Guangzhong Guo, Ziyue Zhang, Jiubing Zhang, Dayang Wang, Sensen Xu, Shuang Wu, Kaiyuan Deng, Yage Bu, Zhiyuan Sheng, Jinliang Yu, Yushuai Gao, Zhaoyue Yan, Ruijiao Zhao, Meiyun Wang, Tianxiao Li, Xingyao Bu

“…We used sequential sequencing of circulating tumor DNA (ctDNA) to compare the somatic mutation profile and clonal evolution of gliomas with different recurrence patterns. …”
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The quadruplex fluorescent quantitative PCR method for the simultaneous detection of respiratory diseases in quail: Pasteurella multocida, Avibacterium paragallinarum, Mycoplasma g... by Haojie Wang, Lihong Xue, Longxi Wang, Yixuan Liu, Jianxing Chen, Yue Sun, Tongqing An, Changwen Li, Hongyan Chen, Changqing Yu, Changyou Xia, He Zhang

“…The sensitivity, specificity, reproducibility, and accuracy of the assay were systematically evaluated.ResultsThe constructed standard curves demonstrated strong linearity (R2 = 1.000, 0.998, 1.000, and 1.000), with high amplification efficiencies (107.09, 91.23, 112.10, and 125.51%, respectively). …”
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Reversal of inflammatory reprogramming by vasodilator agents in pulmonary hypertension by Anna Kirillova, Meena Sethuraman, Xishuang Dong, Almina Kirdar, Gil Speyer, Yassmin Al Aaraj, Annie Watson, Lily K. Schneider, Michael D. Creager, Robert Lafyatis, Satoshi Okawa, Seungchan Kim, Stephen Y. Chan

“…Single-cell profiling of inflammatory cells in blood could clarify these PAH mechanisms. Methods We evaluated a University of Pittsburgh Medical Center cohort consisting of idiopathic PAH (iPAH) and systemic sclerosis-associated PAH (sscPAH) patients and non-PAH controls. …”
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Unveiling fatty acid subtypes: immunometabolic interplay and therapeutic opportunities in gastric cancer by Huahuan Liu, Xin Hu, Xin Hu, Xiangnan Zhang, Yanxin Yao, Liuxing Wu, Ye Tian, Hongji Dai, Kexin Chen, Ben Liu

“…Public data from single-cell RNA sequencing confirmed that the characterized genes were predominantly expressed in endothelial cells and fibroblasts. …”
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Real-world enrollment for a prospective clinico-genomic database using a pragmatic technology-enabled platform by Alexia Exarchos, Ariel B. Bourla, Maneet Kaur, Katja Schulze, Sophia Maund, Yi Cao, Yihua Zhao, Elizabeth H. Williams, Sarah C. Gaffey, Richard Zuniga, Shaily Lakhanpal, Vladan Antic, Michelle Doral, Johanna Sy, Neal J. Meropol, Anne C. Chiang

“…Clinico-genomic datasets, which retrospectively link real-world clinical data to tumor sequencing data, are important resources for biomarker research, which has historically relied on robust research infrastructures exclusive to large academic centers. …”
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EFNA2 Mediates Stiffness-Regulated Hypopharyngeal Cancer Progression by Songqun Hu, Yuanru Liu, Min Cheng, Min Xu, Huiting Liu, Ying Shan, Haosheng Ni, Tian Xia, Qicheng Zhang, Zhenxin Zhang, Bo You

“…Methods Immunohistochemical analysis and cervical enhanced computed tomography (CT) data were used to evaluate correlations among CT values, matrix stiffness, and prognosis, and to develop a prognostic model for HPC. …”
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Ultra-high dose rate radiotherapy overcomes radioresistance in head and neck squamous cell carcinoma by Hong-Shuai Li, Ruo Tang, Hua-Shan Shi, Zi-Jian Qin, Xiao-Yang Zhang, Yun-Fei Sun, Zhi-Gong Wei, Chao-Fan Ma, Liu Yang, Ye Chen, Zhe-Ran Liu, Li-Li Zhu, Wen Yang, Li Yang, Ai-Ning Xu, Zhuo Zhang, Shu-Qing Liao, Jin-Shui Shi, Jian-Jun Deng, Xiao-Zhong He, Xing-Chen Peng

“…Transcriptome sequencing and Olink proteomics were employed to explore the underlying mechanisms involved. …”
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Viral RNA level, serum antibody responses, and transmission risk in recovered COVID-19 patients with recurrent positive SARS-CoV-2 RNA test results: a population-based observationa... by Chao Yang, Min Jiang, Xiaohui Wang, Xiujuan Tang, Shisong Fang, Hao Li, Le Zuo, Yixiang Jiang, Yifan Zhong, Qiongcheng Chen, Chenli Zheng, Lei Wang, Shuang Wu, Weihua Wu, Hui Liu, Jing Yuan, Xuejiao Liao, Zhen Zhang, Xiaolu Shi, Yijie Geng, Huan Zhang, Huanying Zheng, Min Wan, Linying Lu, Xiaohu Ren, Yujun Cui, Xuan Zou, Tiejian Feng, Junjie Xia, Ruifu Yang, Yingxia Liu, Shujiang Mei, Baisheng Li, Zhengrong Yang, Qinghua Hu

“…We performed a population-based observational study to characterize the viral RNA level and serum antibody responses in recurrent-positive patients and evaluate their viral transmission risk. Of 479 recovered COVID-19 patients, 93 (19%) recurrent-positive patients were identified, characterized by younger age, with a median discharge-to-recurrent-positive length of 8 days. …”
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ANTIOXIDANT ACTIVITY AND INHIBITION OF ACETYLCHOLINESTERASE OF INSECT-ASSOCIATED FUNGI FROM THE CLOUD FOREST OF CENTRAL VERACRUZ, MEXICO by Celeste Ricaño-Rodriguez, Irene Lagunes-Apodaca, Manuel Eusebio Medina-Lopez, Alan Couttolenc-Aguirre, Rosario Medel-Ortiz, Cesar Espinoza-Ramirez

“…The culture broth and biomass were extracted to evaluate the antioxidant capacity, acetylcholinesterase inhibition and quantify flavonoids and total phenols. …”
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Revealing key regulatory factors in lung adenocarcinoma: the role of epigenetic regulation of autophagy-related genes from transcriptomics, scRNA-seq, and machine learning by Xianchang Zeng, Lingyun Wei, Lu Lv, Di Wu, Yingying Shen, Xinliang Lu, Xianghui Kong, Zhijian Cai, Jianli Wang, Jianli Wang

“…However, the role of epigenetic-autophagy genes in LUAD’s tumor microenvironment is under-researched.MethodsWe used the “limma”” package to identify differential epigenetic-related genes associated with altered autophagy regulation (A-ERGs) in LUAD. Single-cell RNA sequencing was further employed to evaluate the heterogeneity of immune cells. …”
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A single epidermal stem cell strategy for safe ex vivo gene therapy by Stéphanie Droz‐Georget Lathion, Ariane Rochat, Graham Knott, Alessandra Recchia, Danielle Martinet, Sara Benmohammed, Nicolas Grasset, Andrea Zaffalon, Nathalie Besuchet Schmutz, Emmanuelle Savioz‐Dayer, Jacques Samuel Beckmann, Jacques Rougemont, Fulvio Mavilio, Yann Barrandon

“…Yet current technology makes it difficult to thoroughly evaluate the behaviour of genetically corrected stem cells before they are transplanted. …”
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Injectable elastin-like recombinamer hydrogels with layered degradation rates for osteochondral regeneration in a rabbit model by Desiré Venegas-Bustos, Gonzalo Martínez-Municio, S. Martínez-Páramo, Israel González de Torre, F. Lamus, M.I. Alonso, A. Gato, A.J. Álvarez-Barcia, A. Vega-Castrillo, Mercedes Alberca, J.C. Rodríguez-Cabello

“…After six months in vivo, outcomes were evaluated via glycosaminoglycan quantification, histological, and immunohistochemistry analyses, revealing cartilage-like tissue production. …”
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Liquid biopsy diagnostics for non-small cell lung cancer via elucidation of tRNA signatures by Zhuokun Feng, Masaki Nasu, Gehan Devendra, Ayman A. Abdul-Ghani, Owen T. M. Chan, Jeffrey A. Borgia, Zitong Gao, Hanqiu Zhang, Yu Chen, Ting Gong, Gang Luo, Hua Yang, Lang Wu, Yuanyuan Fu, Youping Deng

“…Methods We developed a machine-learning model to analyze small RNA sequencing data from 1446 tissue samples to identify a diagnostic tRNA signature. …”
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Integrative genomic and single-cell framework identifies druggable targets for colorectal cancer precision therapy by Yanggang Hong, Yanggang Hong, Jiajun Li, Jiajun Li, Nuo Xu, Wanyi Shu, Feng Chen, Yuze Mi, Haigang Geng, Haigang Geng, Qian Li

“…Single-cell and bulk RNA sequencing were used to characterize gene expression within the tumor microenvironment. …”
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PJA2 Suppresses Colorectal Cancer Progression by Controlling HDAC2 Degradation and Stability by Zhihao Chen, Chaofan Peng, Chi Jin, Ye Wang, Tuo Wang, Peng Yang, Wen Peng, Qingyang Sun, Hengjie Xu, Hongxu Nie, Xiaowei Wang, Junwei Tang, Yueming Sun, Yifei Feng

“…The underlying molecular mechanisms of PJA2 action are elucidated using RNA sequencing (RNA‐seq), co‐immunoprecipitation (co‐IP), proximity ligation assay (PLA), and chromatin immunoprecipitation (ChIP). …”
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Shifts in the rhizosphere microbiome and exudation profile of avocado (Persea americana Mill.) during infection by Phytophthora cinnamomi and in presence of a biocontrol bacterial... by Frédérique Reverchon, Mariel García-Meléndez, Edgar Guevara-Avendaño, Oscar Mora-Chávez, Itzel A. Solís-García, Wesley Dáttilo, José A. Guererro-Analco, Alfonso Méndez-Bravo, Juan L. Monribot-Villanueva, Violeta Patiño-Conde, Fernando Pineda-García, Eria A. Rebollar

“…During the infection process, we measured plant morphological and physiological variables and collected rhizosphere soil samples for bacterial and fungal amplicon sequencing, bacterial RNA-seq and metabolomic analyses. …”
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