Astragalus mongholicus polysaccharides alleviate insulin resistance through modulation of PI3K/AKT, TLR4/NF-kB signaling pathway and microbiota in rats with Type 2 Diabetes Mellitu... by Haisheng Yuan, Guoquan Xu, Jingran Liu, Yan Yan, Shimin Zhao, Fujuan Cai, Xiuling Yu, Yuzhen Wang, Minhui Li

“…Histological change was studied by Hematoxylin and eosin (HE) staining. 16S rDNA sequencing was used to detect the changes in gut microbiota. …”
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Urethra-sparing prostate cancer radiotherapy: Current practices and future insights from an international survey by Jennifer Le Guévelou, Paul Sargos, Piet Ost, Filippo Alongi, Stefano Arcangeli, Alejandro Berlin, Pierre Blanchard, Anna Bruynzeel, Olivier Chapet, Alan Dal Pra, Robert T. Dess, Matthias Guckenberger, Andrew Loblaw, Amar U. Kishan, Barbara Alicja Jereczek-Fossa, David Pasquier, Mohamed Shelan, Shankar Siva, Alison C. Tree, Costantinos Zamboglou, Stephane Supiot, Vedang Murthy, Thomas Zilli

“…Results: Magnetic resonance imaging with T2-weighted sequences is the preferred method for urethra contouring (83 % consensus). …”
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Foliar Nitrogen Application Enhances Nitrogen Assimilation and Modulates Gene Expression in Spring Wheat Leaves by Yanlin Yao, Wenyan Ma, Xin Jin, Guangrui Liu, Yun Li, Baolong Liu, Dong Cao

“…Nitrogen (N) critically regulates wheat growth and grain quality, yet the molecular mechanisms underlying foliar nitrogen application remain unclear. This study evaluated the effects of foliar nitrogen application (12.25 kg ha⁻¹) on the growth, grain yield, and quality of spring wheat, as well as its molecular mechanisms. …”
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Virulence and genome analysis of baculovirus isolates from different Lymantria dispar populations by Y. Akhanaev, S. Pavlushin, M. Yakimova, D. Lebedeva, D. Kurenshchikov, Z. Teshebaeva, S. Toshchakov, Y. Vyatkin, A. Kononchuk, A. Ignatieva, Y. Tokarev, V. Soukhovolsky, N. M. Inoue, V. Martemyanov

“…Genomic analysis of novel LdMNPV sequences (LdMNPV-KR, -KG, and -BibJ) detected differences in genome sizes, gene composition, and specific mutations, including the absence of the enhancing factor-1 gene in the LdMNPV-BibJ isolate. …”
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Autoimmune polyglandular syndrome among Chinese celiac disease patients: a survey of 243 individuals in China by Shenglong Xue, Yan Feng, Yan Feng, Tian Shi, Tian Shi, Halina Halike, Halina Halike, Ayinuer Maimaitireyimu, Ayinuer Maimaitireyimu, Adilai Abudurexiti, Adilai Abudurexiti, Jinjin Xie, Shanxia Yao, Feng Gao, Feng Gao

“…APS typing was conducted based on serum antibodies, gene sequencing (AIRE mutation analysis), and comorbidity analysis.ResultsThe overall prevalence of APS in CeD patients was 10.3% (25/243), and the prevalence of different types of APS varied as follows: APS-1: 0.4%, APS-2: 0.4%, APS-3: 8.2%, and APS-4: 1.2%. …”
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Identification of functional SNP associated with sperm quality in porcine ANXA5 that contributes to the growth of immature Sertoli cell by Mingyang Han, Diwen Yao, Yuyang Song, Yuhang Liu, Zhihua Chen, Jialian Li, Fenge Li, Xiuqin Yang, Lihe Dai, Buyue Niu

“…Then, putative causative variants were screened by integrating in silico analysis and DNA sequencing, and the subsequent association analysis was performed in Largewhite boars. …”
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Experimental co-infection of calves with SARS-CoV-2 Delta and Omicron variants of concern by Konner Cool, Natasha N. Gaudreault, Jessie D. Trujillo, Igor Morozov, Chester D. McDowell, Dashzeveg Bold, Taeyong Kwon, Velmurugan Balaraman, Patricia Assato, Daniel W. Madden, Emily Mantlo, Jayme Souza-Neto, Franco Matias-Ferreyra, Jaime Retallick, Gagandeep Singh, Michael Schotsaert, Mariano Carossino, Udeni B. R. Balasuriya, William C. Wilson, Roman M. Pogranichniy, Adolfo García-Sastre, Juergen A. Richt

“…Twenty-four hours post-challenge, two sentinel calves were introduced to evaluate virus transmission. The co-infection resulted in a high proportion of calves shedding SARS-CoV-2 RNA at 1- and 2-days post-challenge (DPC). …”
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A comprehensive risk model of disulfidoptosis-related lncRNAs predicts prognosis and therapeutic implications in bladder cancer by Zhixiong Zhang, Jinghua Zhong, Muhammad Sarfaraz Iqbal, Zhiwen Zeng, Xiaolu Duan

“…The validated study evaluated intricate interactions between functional enrichment, immune cell infiltration, cancer mutation load, and treatment sensitivity. …”
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Promoter methylation and increased expression of PD-L1 in patients with active tuberculosis by Yen-Han Tseng, Sheng-Wei Pan, Jhong-Ru Huang, Chang-Ching Lee, Jung-Jyh Hung, Po-Kuei Hsu, Nien-Jung Chen, Wei-Juin Su, Yuh-Min Chen, Jia-Yih Feng

“…PD-L1 promoter methylation status was evaluated using bisulfite sequencing. Immunohistochemistry (IHC) and immunofluorescence (IF) staining were used to visualize PD-L1- and TET-1-expressing cells in lung tissues from patients with TB and in macrophage cell lines with MTB-related stimulation. …”
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Effects of azithromycin on alleviating airway inflammation in asthmatic mice by regulating airway microbiota and metabolites by DanHui Huang, Lingyan Xie, Tingyue Luo, Lishan Lin, QianNan Ren, Zhaojin Zeng, Haohua Huang, Hua Liao, XiaoDan Chang, Yuehua Chen, Haijin Zhao, Shaoxi Cai, Hangming Dong

“…A house dust mite (HDM)-induced asthmatic mouse model was used to evaluate the effects of azithromycin on airway inflammation and microbiota. …”
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Effect of neurally adjusted ventilator assist versus pressure support ventilation on asynchronies and cardiac function in pediatric liver transplantation by Fabrizio Chiusolo, Giorgia Spinazzola, Roberta Costa, Alessio Franceschini, Francesca Tortora, Francesco Polisca, Emanuele Rossetti, Lucilla Ravà, Marcello Chinali, Vito Fanelli, Giorgio Conti

“…Patients were randomised to one of two crossover sequences of ventilation trials of 40 min each (PSV/NAVA/PSV or NAVA/PSV/NAVA). …”
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Effects of combined temperature and salinity on growth and fatty acid content of two Korean isolates of Akashiwo sanguinea (Dinophyceae) by Ji Yeon Kim, Joo-Hwan Kim, Zhun Li, Kyong Ha Han, Minjae Kim, Minseok Jeong, Kyun-Woo Lee, Ji Hoon Lee, Joo Yeon Youn, Pung-Guk Jang, Kyungsoon Shin, Da Bin Choi, Ji Won Jeon, Hyeon Ho Shin

“…In addition, the effects of combined temperature and salinity on their growth and fatty acid contents were examined to evaluate the value as potential source for commercial application. …”
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Alterations in the Salivary Microbiome and Metabolism in Patients With Carotid Atherosclerosis from Rural Northeast China by Yahong Wu, Liying Xing, Lijie Lu, Shuang Liu, Dan Zhao, Li Lin, Songlin Wang, Chen Li, Yaping Pan

“…We performed full‐length 16S rDNA gene sequencing and untargeted metabolomics of saliva samples from 96 subjects. …”
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Transcriptome analysis of Thevetia peruviana cell suspensions treated with methyl jasmonate reveals genes involved in phenolics, flavonoids and cardiac glycosides biosynthesis by Olmedo Cuaspud, Olmedo Cuaspud, Dary Mendoza, Gigliola Navarro, Gigliola Navarro, Juan Arias, Isabel Calle, Juliana Arcila-Galvis, Juliana Arcila-Galvis, Rafael Eduardo Arango Isaza

“…To deepen our understanding of the effect of MeJA on the secondary metabolism of T. peruviana, transcriptome sequencing was performed on suspension cell culture. …”
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Real world effectiveness of early ensitrelvir treatment in patients with SARS-CoV-2, a retrospective case series by Shuichi Abe, Dhammika Leshan Wannigama, Yu Suzuki, Daisuke Akaneya, Junko Igarashi, Mayu Suto, Kazunori Moriya, Daisuke Ishizawa, Yoshikazu Okuma, Parichart Hongsing, Cameron Hurst, Thammakorn Saethang, Paul G. Higgins, Stephen M. Stick, Anthony Kicic

“…Variants were identified by Sanger sequencing, and outcomes were compared to historical controls. …”
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miPEP31 alleviates sepsis development by regulating Chi3l1-dependent macrophage polarization by Yu Zhou, Yuan Yuan, Xuanqi Yao, Lin Wang, Liangfang Yao, Daolin Tang, Feng Chen, Jinbao Li

“…Using an experimental sepsis model of caecal ligation and puncture (CLP), the impact of mmu-miR-31-5p on survival, organ injury, and inflammation was evaluated. Additionally, the effect of mmu-miR-31-5p on macrophage polarization through Chi3l1 was investigated. …”
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Altered exosomal miRNA profiles in patients with paraneoplastic cerebellar degeneration by Eirik Tveit Solheim, Liv Cecilie Vestrheim Thomsen, Line Bjørge, Shamundeeswari Anandan, Elise Peter, Virginie Desestret, Cecilie Totland, Christian A. Vedeler

“…Receiver operating characteristic curves were used to evaluate biomarker sensitivity and specificity, and miRNA target prediction analysis was employed to elucidate gene targets. …”
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Effect of biodegradable films on microplastic distribution and microbial community composition in paddy soil by Liyin REN, Wei ZHU, Minghang ZHU, Aihua ZHANG, Junling CAI, Lei XU, Kai WANG, Xuejun LIU, Rui JIANG

“…The MP composition was analyzed by Fourier-transform infrared spectroscopy. Metagenomic sequencing was conducted to identify MP degradation genes and characterize microbial communities. …”
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Assisted Gene Flow Management to Climate Change in the Annual Legume Lupinus angustifolius L.: From Phenotype to Genotype by S. Sacristán‐Bajo, C. Lara‐Romero, A. García‐Fernández, S. Prieto‐Benítez, J. Morente‐López, M. L. Rubio Teso, E. Torres, J. M. Iriondo

“…We measured flowering onset, reproductive success, and other plant traits in all resulting lines. In parallel, we sequenced genes related to reproduction, growth, stress, nitrogen, and alkaloids. …”
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Mixing of Two Microbial Consortia in the Search for Stimulating Chromium Depletion by Elcia Margareth Souza Brito, Paola Abigail Martínez-Aldape, Sara Lucia Toscano-Alaniz, César Augusto Caretta, Mónica Jacquelina Martínez-Ramírez, Ramón Eugenio Martínez-Ramírez, Mario Enrique Sandoval-Vergara, Sandra Ixmucamé Concha-Guerrero, Marisol Goñi-Urriza, Alma Hortensia Serafín-Muñoz, Claudia Adriana Ramírez-Valdespino, Rémy Guyoneaud

“…Bacterial synergism between these consortia was evaluated using different compound ratios (C55:C33 ratios of 1:1, 1:2, and 2:1), growing at 50, 100, and 200 mg·L−1 Cr(VI). …”
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