EGFR and K-ras Mutation Analysis in Non-Small Cell Lung Cancer: Comparison of Paraffin Embedded versus Frozen Specimens by Mariëlle I. Gallegos Ruiz, Karijn Floor, Frank Rijmen, Katrien Grünberg, José A Rodriguez, Giuseppe Giaccone

“…Methods: We used PCR amplification and direct sequencing to analyze EGFR and K-ras genes in paired FPE and frozen tumor samples corresponding to 47 NSCLC patients. …”
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Sulforaphane induces cell morphology change and cell apoptosis by activating endoplasmic reticulum stress in glioblastoma by Nan Li, Yan Jiang, Ajun Wang, Tongchao Jiang, Huimin Dai, Chengyu Xia, Tongcui Jiang

“…Additionally, normal human astrocytes (HA) were used to evaluate the selectivity of SFN’s cytotoxicity. In vivo validation was performed using an intracranial glioma xenograft mouse model. …”
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Integrative machine learning model of RNA modifications predict prognosis and treatment response in patients with breast cancer by Tao Wang, Shu Wang, Zhuolin Li, Jie Xie, Qi Jia, Jing Hou

“…Furthermore, single-cell RNA sequencing data were analyzed to understand the biological mechanisms underlying CMRS. …”
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Expression of recombination antimicrobial protein PIL22-PBD-2 in Pichia pastoris and verification of its biological function in vitro by Xian Li, Pengfei Qiu, Menglong Yue, Ying Zhang, Congshang Lei, Jingyu Wang, Xiwen Chen, Xuefeng Qi

“…In this study, the amino acid sequences of PBD-2 and PIL-22 were combined to express the fusion protein in Pichia pastoris, and its biological activity was evaluated in vitro. …”
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Mendelian randomization analysis of inflammatory biomarkers and hepatocellular carcinoma risk: genetic causality and single-cell transcriptomics by GuangXin Shao, Xiao Yun, Hongyue Xie, Beicheng Sun

“…Methods We conducted comprehensive MR analyses using multiple statistical approaches including MR Egger, weighted median, inverse variance weighted, weighted mode, and simple mode methods to evaluate causal relationships between inflammatory biomarkers and HCC risk. …”
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Feature selection and aggregation for antibiotic resistance GWAS in Mycobacterium tuberculosis: a comparative study by Kirill Reshetnikov, Daria Bykova, Daria Bykova, Konstantin Kuleshov, Konstantin Kuleshov, Konstantin Chukreev, Egor Guguchkin, Alexey Neverov, Gennady Fedonin

“…Statistical machine learning (ML) methods are used to infer new genes implicated in drug resistance leveraging large collections of isolates with known whole-genome sequences and phenotypic states for different drugs. …”
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Prevalence, persistence and diversity of Listeria strains with antimicrobial resistance and virulence genes from fresh fruits in Southwest Nigeria by Esther Omobola Areo, Amarachukwu Anyogu, Muinah A. Fowora, Mobolaji O. Omemu, Olusola B. Oyewole, Adewale Olusegun Obadina

“…Listeria spp was identified in 270 fruits: garden egg (90), tomato (90) and watermelon (90), were purchased from thirty markets, in Southwest Nigeria. Listeria spp were evaluated, identified and sequenced. Antimicrobial sensitivity assay (15 antibiotics), eighteen antimicrobial and nine virulence genes were screened for. …”
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Comprehensive transcriptome, miRNA and kinome profiling identifies new treatment options for personalized lung cancer therapy by Shen Zhong, Yvonne Börgeling, Patrick Zardo, Danny Jonigk, Jürgen Borlak

“…We confirmed results based on cross‐linked immunoprecipitation‐sequencing data, and used N = 524 adeno‐ and 497 squamous cell carcinomas as validation sets. …”
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Amazonian manatee critical habitat revealed by artificial intelligence‐based passive acoustic techniques by Florence Erbs, Mike van derSchaar, Miriam Marmontel, Marina Gaona, Emiliano Ramalho, Michel André

“…Using this model, we evaluated manatee acoustic presence over a total of 226 days comprising recording periods in 2021 and 2022. …”
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Heat Stress Response in Ruditapes Decussatus: Transcriptional Regulation of Key Pathways and Novel SNPs in Antioxidant Genes by Dimitrios K. Papadopoulos, Basile Michaelidis, Ioannis A. Giantsis

“…Additionally, the genes catalase and Cu‐Zn sod were characterized for the first time, and along with the metallothionein gene, were sequenced in heat‐resilient and heat‐susceptible individuals to identify polymorphisms potentially associated with thermal tolerance. …”
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Effect of NAM-1 genes on the protein content in grain and productivity indices in common wheat lines with foreign genetic material introgressions in the conditions of Belarus by O. A. Orlovskaya, S. I. Vakula, K. K. Yatsevich, L. V. Khotyleva, A. V. Kilchevsky

“…Full-length NAM-A1 nucleotide sequences of T. dicoccoides k-5199, T. dicoccum k-45926, T. kiharae, and T. spelta k-1731 accessions were established and registered with the international molecular database GenBank. …”
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The impact of the Change4Life Food Scanner app on children’s diets and parental psychological outcomes: a randomised pilot and feasibility study by Sundus Mahdi, Jim Chilcott, Nicola J. Buckland

“…This study investigated (1) the feasibility and acceptability of evaluating the effectiveness of the Food Scanner app in reducing children’s energy (kcal) and sugar (g) intake over a 3-month period, (2) app engagement and (3) the app’s impact on psychological outcomes. …”
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Seagrass Enhalus acoroides extract mitigates obesity and diabetes via GLP-1, PPARγ, SREBP-1c modulation and gut microbiome restoration in diabetic zebrafish by Marcello Mikhael Kadharusman, Rony Abdi Syahputra, Rudy Kurniawan, Edwin Hadinata, Raymond Rubianto Tjandrawinata, Nurpudji Astuti Taslim, Raffaele Romano, Antonello Santini, Fahrul Nurkolis

“…This study aimed to evaluate the anti-obesity and anti-diabetic effects of Enhalus acoroides extract (SEAE) in a zebrafish model of diet- and glucose-induced metabolic dysfunction. …”
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Crop rotation enhances yield and water productivity: Uncovering potential drivers through global field experiment synthesis by Liangang Xiao, Yudi Wang, Chenxi Zhang, Kebing Zhao, Rongqin Zhao, Zhixiang Xie

“…Crop yield, crop water productivity (WPc), and crop evapotranspiration (ETc) represent three essential ecological metrics for evaluating sustainable intensification strategies in cropland management. …”
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Factors influencing endometrial receptivity in women with recurrent implantation failure by Katarzyna Opuchlik, Katarzyna Pankiewicz, Piotr Pierzyński, Janusz Sierdziński, Elina Aleksejeva, Andres Salumets, Tadeusz Issat, Piotr Laudański

“…This study was aimed to evaluate the impact of age and other clinical factors on endometrial receptivity in women with RIF. …”
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Immune-Mediated Bidirectional Causality Between Inflammatory Bowel Disease and Chronic Periodontitis: Evidence from Mendelian Randomization and Integrative Bioinformatics Analysis by Zhijun Feng, Zihan Chen, Xiaoxu Wang, Meijuan Zhou, Shupeng Liu

“…The causal relationships were primarily evaluated using inverse variance weighting (IVW), complemented by multiple sensitivity analyses to assess the robustness of the findings. …”
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Surgical and radiological outcomes of giant cell tumor of the bone: prognostic value of Campanacci grading and selective use of denosumab by Jasminka Igrec, Lisa Jernej, Maria Anna Smolle, Jakob Steiner, Susanne Scheipl, Birgit Lohberger, Andreas Leithner, Iva Brcic

“…Methods We retrospectively analyzed 55 patients with GCTB, focusing on clinicopathologic and radiological findings. Tumors were evaluated using the Campanacci grading system. Immunohistochemical analysis with H3.3 G34W antibody and next-generation sequencing (NGS) were performed to detect H3F3A mutations. …”
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Exploring the Characteristics of Atoxigenic <i>Aspergillus flavus</i> Isolates and Their Biocontrol Impact on Soil Fungal Communities by Yanyan Zhang, Wanning Wang, Chenggui Piao, Wenjin Li, Peter J. Cotty, Shihua Shan, Usman Rasheed, Quirico Migheli, Qing Kong

“…We utilized Illumina sequencing to investigate the changes in soil fungal ecology. …”
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Detection of Mediterranean Black Widow Spider, Latrodectus tredecimguttatus (Rossi, 1790) for the First Time in Jordan by DNA Barcoding and a Case of Envenomation Treated with Fer... by Ehab Eid, Hussein Alnasarat, Said Ahmad Damhoureyeh, Sergio Henriques

“…The clinical process was evaluated including the symptoms, medical treatment, and the use of Ferula assa-foetida as self-medicated traditional medicine. …”
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Mapping heat tolerance QTLs in Triticum durum-Aegilops speltoides backcross introgression lines to enhance thermotolerance in wheat by Navaneetha Krishnan J., Satinder Kaur, Uttam Kumar, Uttam Kumar, Rohtas Singh, Guriqbal Singh Dhillon, Pradeep Kumar Bhati, Parveen Chhuneja

“…The homozygous BILs were evaluated for heat stress tolerance component traits under an optimum environment (OE) and a heat-stressed environment (HE) for the two cropping seasons. …”
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