GLP-1R activation attenuates the progression of pulmonary fibrosis via disrupting NLRP3 inflammasome/PFKFB3-driven glycolysis interaction and histone lactylation by Chenyang Liu, Qun Zhang, Hong Zhou, Linling Jin, Chang Liu, Mingxia Yang, Xinyun Zhao, Wenqiu Ding, Weiping Xie, Hui Kong

“…Methods A silica-induced pulmonary fibrosis mouse model was established to evaluate the protective effects of activating GLP-1R with liraglutide in vivo. …”
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Identification of neutrophil extracellular trap-related biomarkers in ulcerative colitis based on bioinformatics and machine learning by Jiao Li, Yupei Liu, Zhiyi Sun, Suqi Zeng, Caisong Zheng

“…Finally, the expression of core genes in patients was validated using Quantitative real-time PCR (qRT-PCR), and potential therapeutic drugs for UC were explored through drug target databases.ResultDifferential analysis of transcriptomic sequencing data from UC samples identified 29 DEGs related to NETs. …”
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Selection of bispecific antibodies with optimal developability using FcRn‑pH‑HPLC as an optimized FcRn affinity chromatography method by Thomas Müller, Carolin Tasser, Michael Tesar, Ivica Fucek, Ute Schniegler-Mattox, Joachim Koch, Kristina Ellwanger

“…Target and effector antigen-binding domain sequences, their presentation format and orientation within a bispecific antibody alter FcRn retention; tested Fc domain modifications and incorporating stabilizing disulfide bonds had minimal effect. …”
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Fluorodeoxyglucose positron-emission tomography-magnetic resonance hybrid imaging: An emerging tool for staging of cancer of the uterine cervix by Alina Nazir, Robert Matthews, Annapurneswara Chimpiri, Melissa Henretta, Joyce Varughese, Dinko Franceschi

“…The average SUVmax on the pelvis PET sequences was higher with SUV 8.9 ± 5.2 compared to the whole-body PET with SUV 7.8 ± 5.4 but was not statistically significant (P > 0.05). …”
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Correlation between HPV PCNA, p16, and p21 Expression in Lung Cancer Patients by B. F. São Marcos, T. H. A. de Oliveira, C. M. M. Do Amaral, M. T. C. Muniz, A. C. Freitas

“…Purpose. Evaluate if human papillomavirus (HPV) infection in lung cancer patients might be helping cancer development by altering p16, p21, and PCNA, key human genes involved in cell proliferation and tumor development. …”
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The sandfish identity: phylogeographic reconstructions uncover a species complex within the Indo-Pacific distribution of Holothuria (Metriatyla) scabra by Kelly T. Brown, Paul C. Southgate, Nguyen D. Q. Duy, Lindon Havimana, Aviti J. Mmochi, Rose K. Basiita, Madjid Delghandi, Brian Stockwell, Monal M. Lal

“…Two genome-wide (1,500 PAV and 2,000 SNP) loci and one mitochondrial marker (cox1: 69 sequences) were employed in phylogenetic reconstructions, estimations of genetic distance, and assessments of species boundaries and evolutionary history. …”
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Genetic Signatures: CD44 Single-Nucleotide Polymorphisms Affect Cell Surface Expression and Elevate Risk in Head and Neck Squamous Cell Carcinoma by Muhammad Kashif, Shah Jahan, Sadia Minhas, Ali Amar, Romeeza Tahir, Haseeb Nisar, Faheem Shehzad, Abdul Hanan Nagi, Nadeem Afzal

“…DNA from formalin-fixed paraffin-embedded tissue sections was isolated and amplified using targeted primers. Sanger sequencing of the resultant amplified products was performed to determine the genotypes of the CD44 rs13347 and rs187115 SNPs. …”
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Integrated Multi-Omics Profiling Reveals That Highly Pyroptotic MDMs Contribute to Psoriasis Progression Through CXCL16 by Liping Jin, Xiaowen Xie, Mi Zhang, Wu Zhu, Guanxiong Zhang, Wangqing Chen

“…Immune cell infiltration profiles were evaluated via CIBERSORT, while cell-cell communication networks were analyzed by CellChat. …”
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Unveiling ac4C modification pattern: a prospective target for improving the response to immunotherapeutic strategies in melanoma by Jianlan Liu, Pengpeng Zhang, Chaoqin Wu, Binlin Luo, Xiaojian Cao, Jian Tang

“…This research integrated single-cell sequencing data and transcriptomics to pinpoint ac4C-related genes (acRG) linked to melanoma progression and evaluate their clinical implications. …”
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Deep learning–based classification of parotid gland tumors: integrating dynamic contrast-enhanced MRI for enhanced diagnostic accuracy by Kazim Ayberk Sinci, Ilker Ozgur Koska, Yusuf Kenan Cetinoglu, Nezahat Erdogan, Ali Murat Koc, Nuket Ozkavruk Eliyatkin, Cagan Koska, Barkan Candan

“…Abstract Background To evaluate the performance of deep learning models in classifying parotid gland tumors using T2-weighted, diffusion-weighted, and contrast-enhanced T1-weighted MR images, along with DCE data derived from time-intensity curves. …”
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Data-driven projections of candidate enhancer-activating SNPs in immune regulation by Markus Hoffmann, Tiago Vaz, Shreeti Chhatrala, Lothar Hennighausen

“…The protocol involves scanning the entire human genome (hg38) to pinpoint DNA sequences that deviate by only one nucleotide from the canonical binding sites (TTCnnnGAA) for STAT TFs. …”
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Biofilmed multifarious rhizobacterial isolates of tomato rhizosphere of North-Western Himalayas promote plant growth in tomato by Shubham Kaundal, Neerja Rana, Yashwant Kumar, Saleh S. Alhewairini, Jayanthi Barasarathi, Farah Farhanah Haron, Nazih Y. Rebouh

“…The most potent biofilm-producing isolates were identified using 16S rDNA sequencing.ResultsAmong the isolates, 28 solubilized phosphate (up to 91.2% with MB-7), 26 fixed nitrogen, 18 produced hydrogen cyanide, and 16 produced ammonia. …”
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The alleviating effects and mechanisms of Enterococcus faecium Kimate-X and Lactobacillus plantarum Kimate-F combination on canine inflammatory bowel disease by Rui Zhang, Wanjin Hu, Saiwei Zhong, Weiyang Chen, Shuang Xie, Meiru Chen, Qinghua Yu

“…In vivo efficacy was evaluated in dextran sulfate sodium (DSS)-induced colitis models in mice and dogs. …”
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Contribution and regulation of Ustilago maydis in carbohydrate metabolism during maize silage fermentation by Luo Chongyu, Ni Chengcheng, Dong Quanmin, Yang Fuyu

“…This study systematically evaluated the effects of U. maydis infection on the nutritional composition, fermentation characteristics, and mycotoxin accumulation in maize from Gansu (GSHF) and Hebei (HSHF). …”
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Gastrointestinal flora and serum metabolomic elucidation of Astragali Radix water decoction intervention in subclinical bovine mastitis by Jianpeng Yan, Jianpeng Yan, Ke Zhou, Ting Ma, Peng Ji, Yanming Wei

“…Dose-response trials were conducted in Holstein cows (n = 24 SCBM cases; n = 6 healthy controls), along with multi-omics integration, including 16S rRNA sequencing for rumen/feces microbiota and UHPLC-MS metabolomics for serum analysis. …”
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Comprehensive Analysis of N6-Methyladenosine (m6A) RNA Methylation Regulators and Tumour Microenvironment Cell Infiltration Involving Prognosis and Immunotherapy in Gastroesophagea... by Duanrui Liu, Mingjie Yuan, Zongming Wang, Liping Sun, Yusong Fang, Xiaoli Ma, Lulu Zhang, Yuanxin Xing, Jingyu Zhu, Yunyun Liu, Wenshuai Zhu, Shuqin Bao, Yanfei Jia, Yunshan Wang

“…In this study, we examined the TME of GEA using assessments of the RNA-sequencing data focusing on the distinct m6A modification patterns from the public databases. …”
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Akkermansia muciniphila isolated from forest musk deer ameliorates diarrhea in mice via modification of gut microbiota by Yan Deng, Yan Wang, Ying Liu, Xiaoli Yang, Hai Zhang, Xiaochang Xue, Yi Wan

“…After confirmation by 16S rRNA gene sequencing, a series of in vitro tests was conducted to evaluate the probiotic effects of AKK‐D by assessing its reproductive capability, simulated gastrointestinal fluid tolerance, acid and bile salt resistance, self‐aggregation ability, hydrophobicity, antibiotic sensitivity, hemolysis, harmful metabolite production, biofilm formation ability, and bacterial adhesion to gastrointestinal mucosa. …”
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Biodegradation mechanisms of p-nitrophenol and microflora dynamics in fluidized bed bioreactors by Xin Zheng, Yongjie Zhang, Yongjie Zhang, Zhiheng Ye, Zhiyan Pan

“…Therefore, this study developed an anoxic fluidized bed bioreactor (AFBBR) and an anaerobic-aerobic fluidized bed bioreactor (AAFBBR) to evaluate the biodegradation performance and underlying mechanisms of PNP over a period of 90 days. …”
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The effect of ocean alkalinity enhancement on pelagic bacterial communities: focus points derived from a mesocosm experiment by Dominik Antoni, Antje Wichels, Maarten Boersma, Maarten Boersma, Maarten Boersma, Gunnar Gerdts

“…We assessed changes in bacterial community structure with 16S rRNA amplicon sequencing and abundance with flow cytometry, to evaluate responses to alkalinity addition. …”
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Novel cVEMP procedure reveals sexual dimorphism in peak to trough latency by Max Gattie, Max Gattie, Elena V. M. Lieven, Karolina Kluk

“…Human investigations of sex difference in VEMP latency have shown inconsistent results, although there are indicators of sexual dimorphism in vestibular function and a higher reporting rate for vestibular disorder in women than in men.MethodsSex effects in human VEMP were re-evaluated here using a procedure adapting clinical protocols for higher sensitivity. …”
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