MiR-101-3p Promotes Tumor Cell Proliferation and Migration via the Wnt Signal Pathway in MNNG-Induced Esophageal Squamous Cell Carcinoma by Jianding Wang, Wenwen Zhang, Rui Zhang, Hanteng Yang, Yitong Li, Junling Wang, Chengyun Li

“…Thus, the aim of this study was to evaluate the potential function of miR-101-3p in MNNG-induced ESCC. …”
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Marine natural product Methyl mycophenolate inhibits gastric cancer growth through regulating p53 and the downstream pathways by Xiaofang Liu, Ning Xu, Jie Wang, Kaining Chen, Huiwen Ke, Yufen Xu, Dianying Feng, Lishi Xiao, Xiangqi Meng, Shi Chen, Hongyan Yu

“…Methods We screened marine compounds for their inhibitory activity against GC cells using cell viability, colony formation assays, cell cycle analysis, and apoptosis detection. RNA sequencing and KEGG enrichment analysis identified key downstream pathways activated by MMP. …”
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Driver genes as predictive indicators of brain metastasis in patients with advanced NSCLC: EGFR, ALK, and RET gene mutations by Huijuan Wang, Ziqi Wang, Guowei Zhang, Mina Zhang, Xiaojuan Zhang, Haixia Li, Xuanxuan Zheng, Zhiyong Ma

“…Methods Data from 552 patients with advanced NSCLC treated from January 2015 to June 2017 in the Affiliated Cancer Hospital of Zhengzhou University were retrospectively analyzed. Next‐generation sequencing was used to detect mutations in eight reported driver genes and various risk factors were evaluated. …”
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Clinical significance of FOXN3 expression in Indian breast cancer patients by Sheersh Massey, Mohammad Aasif Khan, Safia Obaidur Rab, Syeda Maryam Husain, Asifa Khan, Sadaf, Zoya Mallik, Saad Mustafa, Rahul Kumar, Maria Habib, S. V. S. Deo, Syed Akhtar Husain

“…To identify the genetic aberrations in FOXN3 gene automated DNA sequencing was done. FOXN3 expression study revealed the elevated expression of FOXN3 mRNA in 61.26% of the cases whereas FOXN3 protein was seen to be overexpressed in 59.15% cases. …”
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Establishment and Characterization of a Brain Parenchymal Metastatic Cell Line AlmoR1 Derived From an NSCLC Patient With EGFR‐TKI Resistance by Jingyi Wu, Xiangfei Wu, Jing Wang, Guili Feng, Yuhan Wang, Zide Chen, Wei Wang, Rong Wang

“…The genetic characteristics of the cell line were evaluated by short tandem repeat (STR) profiling and whole‐exome sequencing analysis. …”
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Enteromorpha prolifera soluble dietary fiber alleviates ulcerative colitis through restoration of mucosal barrier and gut microbiota homeostasis by Yuan-Yuan Ding, Yuan-Yuan Ding, Xin-Yi Tang, Yu Tian, Feng-Long Zhang, Xiang Ding, Meng-Chun Qi, Wei Dong, Chen-Guang Liu

“…The severity of UC was evaluated through daily disease activity index (DAI) scoring; quantification of pro-inflammatory cytokines (TNF-α, IL-1β) via ELISA; histopathological analysis of colon sections with H&E staining; immunofluorescence detection of tight junction proteins (ZO-1, occludin); and 16S rRNA sequencing for gut microbiota.ResultsEDFM treatment significantly reduced the expression of pro-inflammatory cytokines (TNF-α and IL-1β), enhanced the expression of tight junction proteins (ZO-1 and occludin), and stimulated mucin (MUC2) production. …”
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Real-time assessment of circulating tumor cells refines the indication for HER2-targeted therapy in metastatic gastric cancer by Yasuaki Kimura, Koichi Suzuki, Sawako Tamaki, Iku Abe, Yuhei Endo, Kosuke Ichida, Yuta Muto, Fumiaki Watanabe, Masaaki Saito, Kazuo Takeda, Toshiki Rikiyama

“…This study aimed to evaluate real-time HER2 expression on circulating tumor cells (CTCs) using the On-chip Sort system. …”
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Isolation and characterization of Acinetobacter phage vAbaIN10 active against carbapenem-resistant Acinetobacter baumannii (CRAB) isolates from healthcare-associated infections in... by Issa Ndiaye, Laurent Debarbieux, Ousmane Sow, Bissoume Sambe Ba, Moussa Moise Diagne, Abdoulaye Cissé, Cheikh Fall, Yakhya Dieye, Ndongo Dia, Guillaume Constantin de Magny, Abdoulaye Seck

“…The phage's lytic activity was evaluated at different multiplicities of infection (MOI): MOI 10, 1, and 10⁻¹. …”
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Genome-wide analyses reveal intricate genetic mechanisms underlying egg production efficiency in chickens by Lizhi Tan, Xinyu Cai, Yuan Kong, Zexuan Liu, Zilong Wen, Lina Bu, Yuzhan Wang, Xiaojun Liu, Zhiwu Zhang, Jianlin Han, Dandan Wang, Yiqiang Zhao

“…The classic traits for evaluating egg-laying performance include age at first egg, egg number, clutch size, laying rate, etc. …”
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Alterations of the paired maternal fecal microbiota and neonatal meconium microbiota in newborns from pregnant women with hypertensive disorders by Heng Yang, Zhijiang He, Jianfen Lai, Jing Yang, Qianrong Huang, Ying Chang, Mingyuan Tian, Hongli Huang

“…This study aimed to characterize the gut microbiota of pregnant women with HDP and evaluate its potential influence on the meconium microbiota of their newborns.MethodsA cohort of 67 pregnant women, including 36 diagnosed with HDP (HDP group) and 31 healthy, age-matched controls (HC group), along with their offspring, were recruited. …”
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Reversing VTN deficiency inhibits the progression of pancreatic cancer and enhances sensitivity to anti-PD1 immunotherapy by Siqi Zhao, Zhaofeng Gao, Lingyu Hu, Yihan Li, Xiaoguang Wang, Xiaoping Li, Minjie Chen, Fei Chen, Zhengwei Song

“…A syngeneic mouse subcutaneous tumor model evaluated the therapeutic efficacy of VTN overexpression combined with anti-PD1 immunotherapy.ResultsVTN was significantly downregulated in pancreatic cancer tissues compared to normal tissues. …”
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Population Genomics and Application for Growth Improvement of Domesticated Asian Seabass <i>Lates calcarifer</i> from Thailand by Bavornlak Khamnamtong, Atra Chaimongkol, Sirikan Prasertlux, Sirithorn Janpoom, Jutaporn Chaimongkol, Sureerat Tang, Wanwipa Ittarat, Putth Songsangjinda, Takashi Sakamoto, Panya Sae-Lim, Sirawut Klinbunga

“…Estimated breeding values (EBVs) for growth of the base population (G0, 51 families, <i>N</i> = 1655) were evaluated. Fish exhibited either high (HEBV, averaged body weight = 1036.80 ± 250.80 g, <i>N</i> = 133) or low (LEBV, averaged body weight = 294.50 ± 167.20 g, <i>N</i> = 147) growth EBVs, and their parental fish (<i>N</i> = 26) were analyzed by Specific Locus Amplified Fragment Sequencing (SLAF-Seq). …”
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Lavender Exosome-Like nanoparticles attenuate UVB-Induced Photoaging via miR166-Mediated inflammation and collagen regulation by Sixu Li, Fei Liu, Song Zhang, Xin Sun, Xiaojing Li, Qiulin Yue, Le Su, Suzhen Yang, Lin Zhao

“…In this study, we isolated lavender exosome-like nanoparticles (LELNs) and evaluated their protective effects against UVB-induced skin photoaging. …”
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Androgens alleviate the depression-like phenotype in female mice by inhibiting AVPR1a in the hippocampal brain region by Shimin Ren, Xian Wang, Xueying Huang, Liyang Chen, Bing Zhang, Yang Li, Xin Huang

“…The mRNA profile of hippocampal tissues from female mice treated with dehydroepiandrosterone (DHEA) was constructed through RNA sequencing (RNA-seq). GO and KEGG pathway analyses were performed on the differentially expressed genes. …”
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Sea buckthorn-derived extracellular vesicles foster bone regeneration through aau-miR168-mediated pathways by Mai Zhao, Xiaolin Chen, Wenyan Wang, Mengying Li, Hui Zhang, Xiuqun Zou, Jiamin Wang, Qian Cong, Xingyuan Ma, Zhaoyuan Hou, Haodong Lin, Hao Jia

“…Bone marrow mesenchymal stromal cells (BMSCs) were treated with SAEVs, and cellular uptake was evaluated through fluorescence microscopy and flow cytometry. …”
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Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclof... by Camille Grandclément, Anne Piram, Marie-Eléonore Petit, Isabelle Seyssiecq, Isabelle Laffont-Schwob, Guillaume Vanot, Nicolas Tiliacos, Nicolas Roche, Pierre Doumenq

“…Thus, DCF removal was investigated using bacteria isolated from aqueous stock solutions of this micropollutant and identified as Bacillus and Brevibacillus species using 16S rRNA gene sequencing. A 100% DCF removal was achieved after 17 hours of experiment at 20°C in a nutrient medium; the biodegradation kinetic followed a pseudo-first order (kbiol = 11 L·gSS−1·d−1). …”
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Tumor-infiltrating lymphocytes in cancer immunotherapy: from chemotactic recruitment to translational modeling by Fatjona Pupuleku Kraja, Vladimir B. Jurisic, Altijana Hromić-Jahjefendić, Nafsika Rossopoulou, Theodora Katsila, Katarina Mirjacic Martinovic, Javier De Las Rivas, Carmen Cristina Diaconu, Árpád Szöőr

“…Special emphasis is placed on preclinical models that evaluate TIL function, including 3D tumor spheroids, organoid co-cultures, syngeneic mouse models, and humanized systems. …”
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Spawning Asynchrony and Mixed Reproductive Strategies in a Common Mass Spawning Coral by Gerard F. Ricardo, Christopher Doropoulos, Russell C. Babcock, Arne A. S. Adam, Elizabeth Buccheri, Natalia Robledo, Julian Uribe‐Palomino, Peter J. Mumby

“…We tagged and sequenced eighteen coral colonies, representing varying degrees of spatial clustering, along a 130‐m stretch of shallow reef slope. …”
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Isolation and characterization of phages ΦZC2 and ΦZC3 against carbapenem-resistant Acinetobacter baumannii, and efficacy of ΦZC3 on A549 cells by Kareem Essam, Azza G. Kamel, Bishoy Maher Zaki, Mohamed Elhadidy, Amal Ahmed Abdel Aziz, Aysam Fayed, Tamer Roshdy, Ayman El-Shibiny

“…ΦZC3 was selected for its broad host range to be evaluated for rescuing A549 cells from MDR A. baumannii infection. …”
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ZEB1 transcription factor promotes immune escape in melanoma by Christophe Caux, Stephane Dalle, Arnaud de la Fouchardière, Laurie Tonon, Maud Plaschka, Valentin Benboubker, Maxime Grimont, Justine Berthet, Jonathan Lopez, Myrtille Le-Bouar, Brigitte Balme, Garance Tondeur, Lionel Larue, Alain Puisieux, Yenkel Grinberg-Bleyer, Nathalie Bendriss-Vermare, Bertrand Dubois, Julie Caramel

“…We thus wondered whether ZEB1 signaling in melanoma cells may promote immune evasion and resistance to immunotherapy.Methods We evaluated the putative correlation between ZEB1 expression in melanoma cells and the composition of the immune infiltrate in a cohort of 60 human melanoma samples by combining transcriptomic (RNA-sequencing) and seven-color spatial multi-immunofluorescence analyses. …”
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