Exploring the relationship between ultra-processed food consumption and gut microbiota at school age in a Brazilian birth cohort by Etiene Dias Alves, Marina Xavier Carpena, Aluísio J. D. Barros, Elena M. Comelli, Lorena López-Domínguez, Robert H. J. Bandsma, Iná da Silva dos Santos, Alicia Matijasevich, Juliana dos Santos Vaz, Romina Buffarini, Isabel Oliveira Bierhals, Maria Carolina Borges, Luciana Tovo-Rodrigues

“…Microbiota data was obtained at age 12 after 16S rRNA gene sequencing of self-collected fecal samples. Linear or logistic regression models evaluated the relationship between age groups and gut microbiota outcomes (alpha diversity, beta diversity and relative abundances at the phylum and genus levels), considering dietary covariates and demographic, socioeconomic, health-related, and behavioral factors. …”
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Bisphenol F exposure induced vascular toxicity through intestinal microbiota imbalance by Jianlong Yan, Yanbin Pan, Huadong Liu, Jie Yuan, Jie Chen, Yannan Gao, Chaolan Lin, Feng Lin, Rongning Wang, Yaqiong He, Caiping Wang, Cong Xu, Tangzhiming Li, Peng Zhang, Yu Lan, Wenming Shao, Xinli Pang, Da Yin, Xin Sun, Weixiang Luo

“…Faecal microbiota transplantation and antibiotic treatment experiments were performed to evaluate the functions of the intestinal microbiota in VC.ResultsWe enrolled consecutively 57 patients. …”
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Teosinte-derived SynCom and precision biofertilization modulate the maize microbiome, enhancing growth, yield, and soil functionality in a Mexican field by Juan Alfredo Hernández-García, Julio S. Bernal, Sanjay Antony-Babu, Lourdes Villa-Tanaca, César Hernández-Rodríguez, Esaú De-la-Vega-Camarillo, Esaú De-la-Vega-Camarillo, Esaú De-la-Vega-Camarillo

“…This SynCom consisted of seven bacterial strains: Serratia nematodiphila EDR2, Klebsiella variicola EChLG19, Bacillus thuringiensis EML22, Pantoea agglomerans EMH25, Bacillus thuringiensis EBG39, Serratia marcescens EPLG52, and Bacillus tropicus EPP72. High-throughput sequencing revealed significant shifts in bacterial and fungal communities across treatments. …”
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Expression and serodiagnostic efficacy of a novel echinococcosis-specific recombinant fusion antigen rAgB8/1-Em18-Eg95 by Yang Xianwei, Wang Tao, Chen Yin, Wang Wentao

“…The echinococcosis-specific antigens Eg95, AgB8/1 and the Em18 gene sequences were fused and expressed as the novel recombinant antigens rAgB8/1-Em18-Eg95 (T3) and rEm18-Eg95 (T2), used for the diagnosis of hydatid disease, prepared into an enzyme-linked immunosorbent reaction (ELISA) kit, and evaluated for their serological diagnostic value. …”
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Designing multi-epitope vaccines against Echinococcus granulosus: an in-silico study using immuno-informatics by Jadoon Khan, Asma Sadiq, May M. Alrashed, Nosheen Basharat, Syed Nadeem Ul Hassan Mohani, Tawaf Ali Shah, Kotb A. Attia, Aamer Ali Shah, Hayat Khan, Ijaz Ali, Arif Ahmed Mohammed

“…In conclusion, the current in-silico multi-epitope vaccine may be evaluated in-vitro, in-vivo, and in clinical trials as an immunogenic vaccine model. …”
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Identification of matrix stiffness-related molecular subtypes in HCC via integrating multi-omics analysis and machine learning algorithms by Hanqi Li, Jiayi Zhang, Yu Shi, Huanhuan Wang, Ruida Yang, Shaobo Wu, Yue Li, Xue Yang, Qingguang Liu, Liankang Sun

“…A matrix stiffness-related signature comprising 57 genes was constructed by evaluating 101 machine learning algorithm combinations. …”
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Deletion of a polyketide synthase phoE induces monoterpenes production in ascidian-derived fungus Diaporthe sp. SYSU-MS4722 by Siwen Yuan, Shitao Zhuang, Yanhong Han, Yuhang Wen, Senhua Chen, Lan Liu, Lan Liu, Qifeng Lin, Qifeng Lin, Yan Yan, Zhizeng Gao, Qilin Wu

“…Compounds 1, 4, 7, and 8 were evaluated for their cytotoxicity and anti-inflammatory effects in human non-small cell lung cancer A549 cells. …”
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RNA editing-based biomarker blood test for the diagnosis of bipolar disorder: protocol of the EDIT-B study by Andrea Miranda-Mendizabal, Diana Vetter, Juan Zambrano, Jeff Zarp, Victor Chavarría, Anna Giménez-Palomo, Meritxell Gonzalez-Campos, Marc Valenti, Lara Walczer Baldinazzo, Sara Siddi, Maurizio Ferrari, Dinah Weissmann, Chantal Henry, Josep Maria Haro, Lars Vedel Kessing, Eduard Vieta

“…The clinical validation performance of the EDIT-B will be evaluated using the area under the curve, sensitivity, specificity, positive and negative predictive values, and likelihood ratios. …”
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Systematic analysis of ZDHHC9 as a potential prognostic and immunotherapy biomarker in breast cancer by Desheng Zhou, Zhenpeng Wu, Yachao Cui, Yachao Cui, Doudou Wang, Hejun Song, Jun Chen, Hong Lin

“…Insights into ZDHHC9 expression across different cell types and its potential oncogenic pathways were derived from scRNA sequencing analysis. Additionally, immunophenoscore (IPS), EaSIeR and immunotherapy cohorts were utilized to predict immunotherapy responses. …”
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circ_0002970 promotes fibroblast-like synoviocytes invasion and the inflammatory response through Hippo/YAP signaling to induce CTGF/CCN1 expression in rheumatoid arthritis by Haoran Kong, Yushuang Qiao, Dahu Qi, Shixin Zhao, Zhiming Cao, Junhao Feng, Yitong Li, Yunke Liu, Tao Liu

“…Differentially expressed circRNAs were identified via RNA sequencing. The expression of circ_0002970 was validated via qRT‒PCR, FISH, and RNase R digestion assays. …”
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Integrating isotopic niche metrics and gut microbiota assembly reveals coexistence mechanisms in subtropical riverine fish by Yaqiu Liu, Xinhui Li, Konstantinos Ar. Kormas, Chunni Kou, Huifeng Li, Yuefei Li, Jie Li

“…Understanding interspecific niche overlap is fundamental for evaluating resource partitioning and competitive interactions among sympatric species. …”
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Emergence of Linezolid Resistance Genes <i>optrA</i> and <i>cfr</i>(D) in an <i>Enterococcus saccharolyticus</i> from Chicken by Xun Gao, Xiao Luo, Ruorou Qian, Guolong Gao, Jinghao Liu, Junhao Hong, Chao Yue, Jian-Hua Liu, Yi-Yun Liu

“…Conjugation and stability experiments were used to evaluate the transferability and stability of the resistance genes. …”
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Chronic ozone exposure induces hippocampal microglia activation by microbial dysbiosis in rat lungs by Qiuyu Yang, Xueshan Cao, Shanshan Li, Xiaoqi Yang, Pengyu Bai, Yuandong Yan, Changcun Qi, Zhaorui Deng, Bailin Wu, Bo Song

“…After 40 days of O3 exposure in rats, distinct changes in the microbial community were identified using 16S rRNA gene sequencing. This was followed by an assessment of the impact of pulmonary microbiota on serum NETs (neutrophil extracellular traps). …”
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SNP loci identification and KASP marker development system for genetic diversity, population structure, and fingerprinting in sweetpotato (Ipomoea batatas L.) by Feiyang Yang, Tao Lang, Jingyu Wu, Cong Zhang, Huijuan Qu, Zhigang Pu, Fan Yang, Ma Yu, Junyan Feng

“…A restriction site-associated DNA sequencing analysis of 60 sweetpotato accessions in this study yielded about 7.97 million SNPs. …”
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Transcriptome analysis of insecticide resistance mechanisms in field populations of the bean flower thrips, Megalurothrips usitatus (Bagnall) by Hongyi Cao, Jiangjiang Yuan, Yanran Wan, Yingxi Tang, Xiaobin Zheng, Jing Wang, Kanghua Qian, Jiuming Feng, Sirui Chen, Youjun Zhang, Qingjun Wu

“…RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) techniques validated key gene functions. …”
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Genotyping of ABCC8, KCNJ11, and HADH in Iranian Infants with Congenital Hyperinsulinism by Somayyeh Hashemian, Reza Jafarzadeh Esfehani, Siroos Karimdadi, Nosrat Ghaemi, Peyman Eshraghi, Najmeh Malekzadeh Gonabadi, Amirhossein Sahebkar, Rahim Vakili, Mohammad Reza Abbaszadegan

“…The present study aimed to evaluate genetic variants in ABCC8, KCNJ11, and HADH genes as causative genes for CHI in the Iranian population. …”
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Prevalence and Clinical Characteristics of NEUROD1‐MODY in Chinese Early‐Onset Type 2 Diabetes Mellitus and a Literature Review by Tianhao Ba, Qian Ren, Siqian Gong, Meng Li, Hong Lian, Xiaoling Cai, Wei Liu, Yingying Luo, Simin Zhang, Rui Zhang, Lingli Zhou, Yu Zhu, Xiuying Zhang, Jing Chen, Jing Wu, Xianghai Zhou, Yufeng Li, Xirui Wang, Fang Wang, Liyong Zhong, Xueyao Han, Linong Ji

“…Methods We performed next‐generation sequencing in 679 patients with EOD to screen rare variants in NEUROD1 exons and evaluated the effects of variants using in vitro experiments. …”
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Unveiling BPS-induced colonic inflammatory injury in female mice: Integrated evidence from colon microbiome and metabolomic analyses by Han Liu, Yutian Wang, Lisi Wei, Jing Xu, Ruirui Wang, Ling-Guo Zhao, Zhi Tang

“…The effects of BPS exposure on female mice were evaluated via oral gavage administration at doses of 0.05 mg/kg/day and 5 mg/kg/day for four weeks. …”
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Neutrophil extracellular traps drive osteoporosis via NCF2-dependent signaling: integrated transcriptomics with mechanistic validation by Xiangyun Guo, Liang Wang, Shuangliu Chen, Chuanrui Sun, Jinran Qin, Qingqing Liu, Yiwen Gan, Yan Li, Zikai Jin, Xu Wei, Yili Zhang

“…Understanding these mechanisms is essential for targeted therapies.Methods We established a rat OP model via bilateral ovariectomy. Transcriptomic sequencing (RNA-seq) identified differentially expressed genes (DEGs), and summary data-based Mendelian randomization (SMR) analysis validated their causal associations with OP. …”
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Detection of actionable mutations in circulating tumor DNA for non-small cell lung cancer patients by Paul van der Leest, Pim Rozendal, Naomi Rifaela, Anthonie J. van der Wekken, Hanneke Kievit, Vincent D. de Jager, Grigory Sidorenkov, Léon C. van Kempen, T. Jeroen N. Hiltermann, Ed Schuuring

“…Tumor tissue next-generation sequencing (NGS) data, performed as routine molecular testing in the clinical setting, were retrieved from the national pathology registry for 132 patients. …”
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