Early reduction in gut microbiota diversity in critically ill patients is associated with mortality by Hannah Wozniak, Nadia Gaïa, Vladimir Lazarevic, Christophe Le Terrier, Tal Sarah Beckmann, Eleonora Balzani, Martin Urner, Jérôme Pugin, Jacques Schrenzel, Claudia-Paula Heidegger, Gut Microbiota working group

“…Full-length 16S rRNA gene sequencing was performed for gut microbiota analysis, with α-diversity measured using the Shannon index. …”
Get full text

The Co-Existence of <i>mcr-1.1</i> and <i>mcr-3.5</i> in <i>Escherichia coli</i> Isolated from Clinical Samples in Thailand by Panida Nobthai, Sirigade Ruekit, Dutsadee Peerapongpaisarn, Prawet Sukhchat, Brett E. Swierczewski, Nattaya Ruamsap, Paphavee Lertsethtakarn

“…In this study, 178 multi-drug resistant (MDR) <i>Escherichia coli</i> isolates collected from clinical samples at Queen Sirikit Naval Hospital, Chonburi, Thailand, were evaluated for colistin resistance. Of these, six were identified as <i>mcr</i> gene carriers, mediating colistin resistance. …”
Get full text

CHILD syndrome combined linear porokeratosis in a patient with a good response to the topical lovastatin/cholesterol ointment by Kai Chen, Bin Hu, Qiang Chen, Fei Su, Liu-Qing Chen

“…Clinical and laboratory examination, and Whole-Genome Sequencing (WGS) analysis of the NSDHL and PMVK gene were performed. …”
Get full text

Compound probiotics alleviate hyperuricemia-induced renal injury via restoring gut microbiota and metabolism by Ping Liu, Ping Hu, Meiping Jin, Weiqian Sun, Jiajun Wu, Yuyan Tang, Danye Shi, Ting Xie, Yijing Tong, Lusheng Huang, Dongliang Zhang, Hui Zheng, Xudong Xu, Haidong He

“…Gut microbiota composition and metabolite profiles were evaluated using 16 S rRNA gene sequencing and non-targeted metabolomics of fecal samples.Our findings demonstrate that the compound probioticS effectively attenuated hyperuricemia-associated renal dysfunction and interstitial fibrosis. …”
Get full text

Dracocephalum moldavica L. tea alleviates high-fat diet-induced hyperlipidemia in rats via gut microbiota and lipid metabolism by Qinyu Li, Siqi Li, Aruhan Chen, Congying Huang, Jiayin Chang, Na Zhang, Minhui Li

“…Intestinal content samples were analyzed using 16S rRNA sequencing technology to determine differences in intestinal bacterial composition. …”
Get full text

Enhanced efficacy of immune checkpoint inhibitors by folate-targeted multifunctional drug through synergistic therapy inducing ferroptosis and immunogenic cell death in bladder can... by Yibo Shi, Guangrui Fan, Enguang Yang, Yuanfeng Zhang, Hui Ding, Junqiang Tian, Liang Cheng, Hanzhang Wang, Tianzhi Hao, Baodui Wang, Zhiping Wang

“…Specific mechanisms were investigated through transcriptome sequencing and validation at both cellular and animal levels was conducted. …”
Get full text

Effects of 5-Methyl-2′-Deoxycytidine in G-Quadruplex Forming Aptamers d(G₃C)₄ and d[GCG₂(CG₃)₃C]: Investigating the Key Role... by Veronica Esposito, Daniela Benigno, Carla Aliberti, Camilla Esposito, Elisabetta Panza, Antonella Virgilio, Aldo Galeone

“…Furthermore, the resistance to nucleases and the antiproliferative activity of these <b>mC</b>-containing derivatives against HCT116 (human colorectal carcinoma) and T24 (human bladder carcinoma) cell lines have been evaluated. In most of the cases, STAT and STATB derivatives inhibit cell proliferation to different extents, although to a lesser degree than the unmodified parent sequences. …”
Get full text

The potential of low‐intensity pulsed ultrasound to apply the long‐term ovary protection from injury induced by 4‐vinylcyclohexene diepoxide through inhibiting granulosa cell apopt... by Juan Deng, Juan Qin, Guolin Song, Chenghai Li, Wentao Tang, Yilin Tang, Xinfang Xiao, Liu Wu, Sicheng He, Yiqing Zhou, Junfen Li, Yan Wang

“…The long‐term effectiveness of LIPUS in protecting against ovarian injury was evaluated through ELISA, estrous cycle monitoring, fertility testing, and behavioral analysis. …”
Get full text

Prognostic nutrition index reveals LAG3 in cytotoxic CD8+ T cells and MHC class II in gastric cancer cells by Chikanori Tsutsumi, Kenoki Ohuchida, Masaki Imamura, Bryan Tan, Yuki Shimada, Kiwa Son, Takaaki Kosai, Naoki Katayama, Yuki Mochida, Sayuri Hayashida, Chika Iwamoto, Nobuhiro Torata, Kohei Horioka, Koji Shindo, Yusuke Mizuuchi, Naoki Ikenaga, Kohei Nakata, Yoshinao Oda, Masafumi Nakamura

“…Materials and methods We retrospectively analyzed clinical data from 796 GC patients who underwent radical gastrectomy to identify which previously reported nutritional index had the greatest impact on prognosis. Single-cell RNA sequencing was performed on 38 GC tissues, and multiplex immunofluorescence staining was conducted on 59 GC tissues to evaluate the relationship between nutritional indices and IC molecule expression in cytotoxic CD8-positive T cells. …”
Get full text

Prediction of soft tissue sarcoma grading using intratumoral habitats and a peritumoral radiomics nomogram: a multi-center preliminary study by Bo Wang, Hongwei Guo, Meng Zhang, Yonghua Huang, Lisha Duan, Chencui Huang, Jun Xu, Hexiang Wang

“…A nomogram was created by merging the best radiomics signature with the peritumoral edema. We evaluated the performance and clinical value of the model using area under the curve (AUC), calibration curves, and decision curve analysis.ResultsA multi-layer perceptron classifier model based on intratumoral habitats and peritumoral features combined gave the best radiomics signature, with an AUC of 0.856 for the validation cohort. …”
Get full text

Orthopedic infections associated with distinct Acinetobacter strains in rural area of Qingdao, China by Ying Wang, Dan Zhao, Yeshun Fan, Yanxiang Cui, Yingdi Wang, Xiaoxuan Guan, Linhong Yu, Shunqian Yuan, Lan Wang, Jianqiang Hu, Yisong Li, Wenbo Xia, Jie Liu

“…This study reported orthopedic infection cases associated with different Acinetobacter species and characterized the genomes of the culture isolates to evaluate their potential impact on the clinical treatment.MethodsNine in-patients with A. baumannii-calcoaceticus complex identified by culture during hospitalization were enrolled by the Orthopedics Department from a local hospital in Qingdao, China. …”
Get full text

Telomere Length and Genetic Variations in Acquired Pediatric Aplastic Anemia: A Flow-FISH Study in Korean Patients by Yuna Hong, Jong-Mi Lee, Chaeyeon Lee, Duyeon Na, Jin Jung, Ari Ahn, Jae Won Yoo, Jae Wook Lee, Nack-Gyun Chung, Myungshin Kim, Yonggoo Kim

“…Telomere lengths were measured using Flow-FISH, and relative telomere length (RTL) and delta RTL assessments were conducted. Genetic evaluations included karyotyping, chromosome breakage tests and clinical exome sequencing (CES) to identify inherited bone marrow failure syndrome (IBMFS)-associated genetic variants. …”
Get full text

Probiotic potential of Phocaeicola coprocola in modulating learning and memory behaviors in the honeybee model by Mengqi Xu, Mengqi Xu, Xiaohan Zhang, Xiaohan Zhang, Xi Luo, Guanzhou Zhou, Guanzhou Zhou, Nana Zhang, Xiaoyan Chi, Xiaoyan Chi, Rongrong Ren, Lihua Peng, Gang Sun, Yunsheng Yang, Yunsheng Yang

“…However, its functional roles and underlying mechanisms remain poorly understood.MethodsWe evaluated the effects of P. coprocola on cognitive performance using the honeybee (Apis mellifera) as a novel model for the microbiota–gut–brain axis. …”
Get full text

EZH2 inhibition and 5-azacytidine enhance antitumor immunity in PTEN-deficient glioblastoma by activation viral mimicry response by Xiao Xiao, Yong Li, Dandan Zhu, Huolun Feng, Zuda Huang, Jieqing Guo, Jiabin Zheng, Fan Xing, Zeying Li, Liangying Zheng, Fa Ling

“…We evaluated the effects of 5-azacytidine (5-AZA) monotherapy and its combination with EZH2 inhibition (EZH2i) on ERV reactivation, type I interferon (IFN) responses, and TME remodeling. …”
Get full text

Elucidating the Mechanism of Temporal Adaptation to Hydrogen Peroxide‐Induced Oxidative Stress in Corynebacterium glutamicum by Chang Yu, Wenjing Hu, Xiaoyu Li, Yu Lei, Dandan Gao, Meng Wang, Ping Zheng, Yan Zhu, Jibin Sun

“…RNA sequencing (RNA‐seq) was employed to analyse gene expression changes after exposure to 720 mM H2O2. …”
Get full text

Frequency and Prognostic Impact of Local Ablation Therapy for Oligoprogression in Non‐Small Cell Lung Cancer by Daisuke Morinaga, Jun Sakakibara‐Konishi, Ryohei Kamada, Masahiro Kashima, Kosuke Tsuji, Shotaro Ito, Megumi Furuta, Tetsuaki Shoji, Yuta Takashima, Hidenori Kitai, Yasuyuki Ikezawa, Hiroshi Taguchi, Tatsuya Kato, Yoshiki Shinomiya, Kanako C Hatanaka, Yutaka Hatanaka, Satoshi Konno

“…The characteristics of patients who may benefit from LAT or their genetic background remain unclear. This study evaluated the frequency, clinicopathological characteristics, and efficacy of LAT in the treatment of OP. …”
Get full text

Isolation, Characterization, and Preliminary Application of Staphylococcal Bacteriophages in Sichuan <i>Paocai</i> Fermentation by Xia Lin, Chunhui Deng, Luya Wang, Yue Shu, Shengshuai Li, Yunlong Song, Hong Kong, Ziwei Liang, Lei Liu, Yu Rao

“…The morphological features of the phages were characterized using transmission electron microscopy (TEM), while key biological properties such as one-step growth kinetics were systematically evaluated, ultimately verifying their taxonomic placement within the Caudoviricetes class. …”
Get full text

Characteristics of Egg Production and Discovery of the Prolactin Gene in Indigenous Tropical White Leghorn Layer Chickens of Indonesia by N Pratiwi, T Sartika, Komarudin, T Haryati, T Kostaman, N Azizah, A Sukmara, B Brahmantiyo, Isbandi, H Zaenal, H Nuraini, H Khasanah, MF Ridhillah, F Saputra

“…This research aims to evaluate the egg production traits of WL chickens at the IRIAP (Indonesian Research Institute for Animal Production). …”
Get full text

Kynurenic acid derived from mesentery regulates mesenteritis and colitis via inducing white adipose browning in Crohn’s disease by Yongheng Wang, Ritian Lin, Fangtao Wang, Huijun Fu, Xia Wang, Fengshan Jin, Qiao Wang, Weigang Shu

“…Interleukin-10 gene knockout (IL-10−/−) and 2,4,6-trinitrobenzenesulphonic acid solution (TNBS)-induced colitis mice were used to evaluate the effects of kynurenic acid (KYNA) on mesenteritis and colitis. …”
Get full text

SARS-CoV-2 Genomic Variants and Their Relationship with the Expressional and Genomic Profile of Angiotensin-Converting Enzyme 2 (ACE2) and Transmembrane Serine Protease 2 (TMPRSS2) by Henrique Borges da Silva Grisard, Marcos André Schörner, Fernando Hartmann Barazzetti, Julia Kinetz Wachter, Vilmar Benetti Filho, Rafael Emmanuel Godoy Martinez, Christinni Machado Venturi, Gislaine Fongaro, Maria Luiza Bazzo, Glauber Wagner

“…Therefore, this study evaluated the influence of different variants in ACE2/TMPRSS2 expressional and genomic profiles. …”
Get full text