Intranasal influenza virus-vectored vaccine offers protection against clade 2.3.4.4b H5N1 infection in small animal models by Ying Liu, Shaofeng Deng, Shuang Ren, Rachel Chun-Yee Tam, Siwen Liu, Anna Jinxia Zhang, Kelvin Kai-Wang To, Kwok-Yung Yuen, Honglin Chen, Pui Wang

“…It is crucial to further evaluate the DelNS1-H5N1 LAIV system to prepare for potential future H5N1 outbreaks in humans.…”
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Effects of different microplastic types on soil physicochemical properties, enzyme activities, and bacterial communities by Wenjie Guo, Zhiwei Ye, Yanna Zhao, Qianle Lu, Bin Shen, Xin Zhang, Weifang Zhang, Sheng-Chung Chen, Yin Li

“…Results of 16S rRNA gene high throughput sequencing showed that MP exposure had little effect on soil microbial alpha diversity, but that PHA contamination significantly reduced ACE, Chao1, and Shannon index values. …”
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Front-line liquid biopsy for early molecular assessment and treatment of hospitalized lung cancer patients by Francesca Parisi, Giuseppa De Luca, Manuela Mosconi, Sonia Lastraioli, Chiara Dellepiane, Giovanni Rossi, Silvia Puglisi, Elisa Bennicelli, Giulia Barletta, Lodovica Zullo, Sara Santamaria, Marco Mora, Alberto Ballestrero, Fabrizio Montecucco, Andrea Bellodi, Lucia Del Mastro, Matteo Lambertini, Emanuela Barisione, Giuseppe Cittadini, Elena Tagliabue, Francesco Spagnolo, Marco Tagliamento, Simona Coco, Mariella Dono, Carlo Genova

“…Background: Molecular characterization is pivotal for managing non-small cell lung cancer (NSCLC), although this process is often time-consuming and patients’ conditions might worsen while molecular analyses are processed.Our primary aim was to evaluate the performance of “up-front” next-generation sequencing (NGS) through liquid biopsy (LB) of hospitalized patients with newly detected lung neoplasm in parallel with conventional diagnosis. …”
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DIRAS1 Drives Oxaliplatin Resistance in Colorectal Cancer via PHB1-Mediated Mitochondrial Homeostasis by Min Long, Qian Ouyang, Jingyi Wen, Xuan Zeng, Zihui Xu, Shangwei Zhong, Changhao Huang, Jun-Li Luo

“…Methods: The effects of DIRAS1 on CRC cell proliferation and migration were evaluated using MTT, wound healing, and colony formation assays. …”
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Targeting NAD + biosynthesis suppresses TGF-β1/Smads/RAB26 axis and potentiates cisplatin cytotoxicity in non-small cell lung cancer brain metastasis by Liyun Zhou, Zhiying Li, Shengli Zhou, Bin Wang, Zhen Liang, Sen Hu, Hang Zhang, Lin Duan, Dongxu Zhao, Luyao Cheng, Hang Ren, Hiroaki Wakimoto, Ming Li

“…To explore impact on downstream signaling, RNA sequencing was used in NAMPT inhibitor-treated and control cells, followed by validation with genetic knockdown, western blot and qRT-PCR. …”
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Genetic structure revealed by SNPs for wild rice Zizania latifolia along a latitudinal gradient and its conservation implications by Godfrey Kinyori Wagutu, Xiangrong Fan, Miriam Chepkwemoi Tengwer, Yanling Liu, Wei Li, Yuanyuan Chen, Wei Wang

“…In this study, using restriction-site associated DNA sequencing (RAD-seq), the genetic structure and local adaptation were evaluated on 60 wild samples composed of 10 Z. latifolia populations collected along a latitudinal gradient and 6 cultivated samples from different cultivars in central China. …”
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M2-like macrophages dictate clinically relevant immunosuppression in metastatic ovarian cancer by Vladimir Benes, Ladislav Pecen, Jelena Pistolic, Lorenzo Galluzzi, Iva Truxova, Radek Špíšek, Lenka Kasikova, Michal Hensler, Petr Skapa, Jan Laco, Ivan Praznovec, Tomas Brtnicky, Lukas Rob, Catherine Sautes-Fridman, Ales Ryska, Jitka Fucikova, Wolf Herve Fridman, Vít Drochýtek, Sarka Vosahlikova, Karel Fiser, Jana Rakova, Tereza Lanickova, Irena Moserova, Martina Rehackova, Ludek Sojka

“…Here, we explore the clinical relevance of the immunological configuration of HGSC metastases.Methods RNA sequencing was employed on 24 paired primary tumor microenvironment (P-TME) and metastatic tumor microenvironment (M-TME) chemotherapy-naive HGSC samples. …”
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Rapid generation and characterization of recombinant HCoV-OC43-VR1558 infectious clones expressing reporter Renilla luciferase by Fei Ye, Na Wang, Qiongge Guan, Mengwei Wang, Jiewei Sun, Desheng Zhai, Baoying Huang, Ying Zhao, Wenjie Tan

“…The genetic stability of the recombinant HCoV-OC43 was assessed through viral genome sequencing following passaging in BHK-21 cells. These reporter viruses were validated as screening tools for inhibitors in vitro by evaluating the antiviral activities of remdesivir and chloroquine. …”
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Role Played by Oil Emplacement in Controlling Pore Network Evolution of Tight Sandstones by Huifang Hu, Yang Ju, Chenyang Zhao, Miaozhi Jing, Liang Sun, Wei Sun, Dengke Liu, Lin Yang, Yue Jing, Guoqiang Huang

“…Whether oil emplacement and diagenetic sequences provoke, deteriorate, or have no effect on pore network evolution, as implied by recent tests and theoretical analysis, are critical factors in forecasting hydrocarbon exploration and development potentials. …”
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Remote photobiomodulation ameliorates behavioral and neuropathological outcomes in a rat model of repeated closed head injury by Chongyun Wu, Meng Li, Zhe Chen, Shu Feng, Qianting Deng, Rui Duan, Timon Cheng-Yi Liu, Luodan Yang

“…Additionally, 16S rDNA amplicon sequencing analyzed changes in the lung microbiome following remote PBM treatment. …”
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Exploring the role of neutrophils in inflammatory pain hypersensitivity via single-cell transcriptome profiling by Kai Ding, Xing Liu, Bin Zeng, Songyu Liu, Lu Zhang, Bo Li, Jinyi Zhou, Xiaosan Su, Jun Wang

“…The role of the CXCR2 target gene and NAMO in modulating post-operative and inflammatory pain was then evaluated, exploring potential mechanisms.ResultsscRNA-seq revealed a significant increase in neutrophils and a decrease in monocytes among CD11b+ cells following surgery and CFA treatment. …”
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Icarisid Ⅱ modulates mitochondrial dynamics for anti-HBV activity by Zhengyun Liu, Haiyan Yang, Juan Wen, Dongyan Xiao, Dongyan Xiao, Wan Yu, Guo Luo, Qihai Gong, Huan Wang

“…The livers of ICS II-treated HBV-infected mice were taken for transcriptome sequencing to screen for different genes, and the results were verified by Western Blot. …”
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A protocol for identifying universal reference genes within a genus based on RNA-Seq data: a case study of poplar stem gene expression by Qi Xie, Umair Ahmed, Cheng Qi, Kebing Du, Jie Luo, Pengcheng Wang, Bo Zheng, Xueping Shi

“…The expression stability of selected genes was further evaluated using ΔCt, geNorm, NormFinder, and BestKeeper. …”
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Gypsum and Tillage Practices for Combating Soil Salinity and Enhancing Crop Productivity by Njomza Gashi, Zsombor Szőke, Antal Czakó, Péter Fauszt, Péter Dávid, Maja Mikolás, László Stündl, Ferenc Gál, Judit Remenyik, Zsolt Sándor, Melinda Paholcsek

“…Soil salinity is a major global challenge, reducing fertility and crop productivity. This study evaluated the effects of various soil management practices on the physical, chemical, and microbial properties of saline soils. …”
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Molecular diagnostics for perioperative microbial identification in periprosthetic joint infection: A scoping review and proposal of a diagnostic flow chart by Pier Francesco Indelli, Trifon Totlis, Bruno Lovreković, Michael Engl, Bruno Violante, Pawel Skowronek, Guillaume Demey, Stefano Ghirardelli, Chiara Maci, Antonella Castagna, Laura Schianchi, Chiara Tassan Din, James Harty, Goksel Dikmen, Christian Schaller, Marko Ostojić

“…The authors conducted a scoping review to evaluate the current state regarding the use of molecular diagnostics in the decision‐making process for the surgical treatment of PJI and to create a flowchart based on molecular diagnostics. …”
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Comparison of two polymerase chain reaction assays, DaAn Gene (DaAn Gene 2019-ncov) and Cepheid (Cepheid Xpert Xpress SARS-CoV-2) for Delta and Omicron variants of SARS-CoV-2 detec... by Martin Maidadi-Foudi, Marie Atsama-Amougou, Celestin Godwe, Emilande Guichet, Nadine L. Boutgam, Guy Roland Awoundza Metomo, Oumarou Goni Hamadama, Gilles-Fils Woguia, Yannick F. Ngoume, Dowbiss Meta Djomsi, Roméo Brice D. Djounda, Modeste Ngamaleu, Hillary Tene, Livo Esemu, Joseph Fokam, Marie-Claire Okomo, Boyomo Onana, Wilfred Mbacham, Marcel Tongo, Eric Delaporte, Martine Peeters, Ahidjo Ayouba, Charles Kouanfack

“…Samples with a cycle threshold (CT) ≤ 28 were sequenced. Cohen’s kappa coefficient assessed assay agreement. …”
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High expression of LIMD2 predicts a poor prognosis and promotes migration of colon cancer cells by Jianjun Wang, Fengjuan Cai, Guodong Liu, Jingtao Zhang, Dongdong Li, Yujie Cai, Yu Wang, Wanjun Xu, Xiaoyan Wang, Xiaomin Li

“…Additionally, RNA sequencing (RNA-seq), co-immunoprecipitation (CoIP), silver staining, and mass spectrometry were performed to uncover the role of LIMD2 in colon cancer cell migration. …”
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SAR1A Induces Cell Growth and Epithelial–Mesenchymal Transition Through the PI3K/AKT/mTOR Pathway in Head and Neck Squamous Cell Carcinoma: An In Vitro and In Vivo Study by Shizhen Fang, Jie Wang, Tianyi Liu, Yang Jiang, Qingquan Hua

“…Transcriptome sequencing and KEGG enrichment pathway analysis revealed evident alterations in the PI3K/AKT/mTOR(PAM) pathways. …”
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Respiratory microbiome and metabolome features associate disease severity and the need for doxycycline treatment in children with macrolide-resistant Mycoplasma pneumoniae-mediated... by Wei-Chao Liao, Wei-Chao Liao, Shiao-Wen Li, En-Wei Hsing, Sung-Han Hsiao, Ian Yi-Feng Chang, Yin-Cheng Chen, Yi-Yin Chen, Yi-Jiun Pan, Yu-Chia Hsieh

“…We compared respiratory microbiome and metabolites based on patients’ later development of prolonged fever and the need for doxycycline treatment (DT, n = 57) and the cured control without fever or doxycycline treatment (WDT, n = 35) by using 16S rRNA-based sequencing and untargeted metabolome analysis.ResultsSignificantly higher diversity and different respiratory microbiomes were evaluated in WDT patients in contrast to DT patients. …”
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Saliva-derived extracellular vesicles: a promising therapeutic approach for salivary gland fibrosis by Boyuan Peng, Xufei Guo, Jialing Kang, Shijiao Pan, Lili Wei, Lianhao Wang, Bo Li, Guangli Han, Yong Cheng

“…Network analysis of exosomal miRNAs and integration of RNA sequencing with sEV miRNA-seq were performed to identify key regulatory pathways. …”
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