Identification of Stable Reference miRNAs for miRNA Expression Analysis in Adult Neurogenesis Across Mouse and Human Tissues by Daniella Liana Levitis, Julia Si, Kushal Ravishankar, Michal Toborek, Minseon Park

“…Six miRNAs—miR-181d-5p, miR-93-5p, miR-103-3p, let-7d-5p, miR-26a-5p, and miR-125a-5p—demonstrated high stability and were evaluated for their suitability as endogenous controls across multiple experimental conditions. …”
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ASSESSMENT OF THE IMPACT OF VANILLIN AND QUERCETIN ON THE DEGREE OF FEED DIGESTABILITY AND THE RUMEN MICROBIOME OF CATTLE by Ludmila V. Vlasenko, Kseniya N. Atlanderova, Galimzhan K. Duskaev

“…The goal of the study was to evaluate the effect of vanillin and quercetin on the degree of digestibility of feed and the microbiome of the rumen of cattle. …”
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Integrated analysis of polytranscriptomics reveals TNFSF ligand genes in pancreatic cancer prognosis and immune regulation by Zhaoda Deng, Lincheng Li, Zitong Yang, Guineng Zeng, Rong Liu

“…We analyzed single-cell RNA sequencing data from the GSE212966 dataset to assess the expression of TNFSF ligands across immune cell types. …”
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Xenodiagnosis in the wild: A methodology to investigate infectiousness for tick-borne bacteria in a songbird reservoir by Jens Zarka, Dieter Heylen, Hein Sprong, Manoj Fonville, Joris Elst, Erik Matthysen

“…Here we describe and evaluate a methodology to quantify infectiousness for tick-borne pathogens in individual wild great tits (Parus major), involving captures and recaptures of targeted individuals. …”
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Variation in Botryosphaeriaceae from Eucalyptus plantations in YunNan Province in southwestern China across a climatic gradient by Guoqing Li, Bernard Slippers, Michael J. Wingfield, Shuaifei Chen

“…Isolates were identified by comparing DNA sequences of the internal transcribed spacer ribosomal RNA locus (ITS), partial translation elongation factor 1-alpha (tef1), β-tubulin 2 (tub2) and DNA-directed RNA polymerase II subunit (rpb2) genes, and combined with their morphological characteristics. …”
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Integrating machine learning and neural networks for new diagnostic approaches to idiopathic pulmonary fibrosis and immune infiltration research. by Yali Guo, Qian Jin, Yi Kang, Wenwen Jin, Ying Liu, Qian Chen, Jian Liu, Yu Guang Wang

“…Further validation of the expression of these genes through transcriptomic sequencing data from IPF and normal group animal models. …”
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Deciphering of differences in gut microbiota and plasma metabolites profile between non-obese and obese Golden Retrievers dogs by Yazhen Cai, Huayu Tang, Guilin Xiang, Hongyu Yi, Jie Zhong, Zhaoxi Xie, Qinfeng Hu, Romaissaa El Bouhi, Pan Zhou, Yong Zhang, Honglin Yan

“…The fecal microbiome and plasma metabolome were determined using 16S rRNA amplicon sequencing and liquid chromatography-mass spectrometry, respectively.ResultsResults showed that the alanine aminotransferase activity and total bilirubin concentration, which have been measured using serum biochemistry analysis, were higher in the Obe group than in the Ctrl group (p < 0.05). …”
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Elucidation of the genetic architecture of water absorption capacity in hard winter wheat through genome wide association study by Meseret A. Wondifraw, Zachary J. Winn, Scott D. Haley, John A. Stromberger, Emily E. Hudson‐Arns, R. Esten Mason

“…Individuals were genotyped using genotyping‐by‐sequencing to detect single nucleotide polymorphisms across the wheat genome. …”
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Integrated Bioinformatic Analyses Reveal Thioredoxin as a Putative Marker of Cancer Stem Cells and Prognosis in Prostate Cancer by Shigeru Sugiki, Tetsuhiro Horie, Kenshiro Kunii, Takuya Sakamoto, Yuka Nakamura, Ippei Chikazawa, Nobuyo Morita, Yasuhito Ishigaki, Katsuhito Miyazawa

“…Methods: We reanalyzed 2 single-cell RNA sequencing data of prostate cancer (PCa) tissues using Seurat. …”
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Exploring the Role of the KCNK1 Potassium Channel and Its Inhibition Using Quinidine in Treating Head and Neck Squamous Cell Carcinoma by Hyun Woo Baek, Eunjung Han, Kyoung Ho Oh

“…We established primary cell cultures from head and neck cancer tissues and employed the FaDu cell line for in vitro studies, modulating KCNK1 expression through overexpression and knockdown techniques. We evaluated cell migration, invasion, and proliferation. …”
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Protein biomarkers for bipolar II disorder are correlated with affective cognitive performance by Sheng-Yu Lee, Sung-Chou Li, Tzu-Yun Wang, Ru-Band Lu, Liang-Jen Wang, Kuo-Wang Tsai

“…Plasma concentrations of the aforementioned proteins were measured, and cognitive performance was evaluated using the BACA. Results Our findings indicated that the BD-II group scored significantly lower than the control group across several BACA subtests, including Digit Sequencing, Symbol Coding, Verbal Memory, and Verbal Fluency, and in overall composite scores and all subtests of the Affective Interference Test (AIT) and Emotion Inhibition Test (EIT). …”
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Detection rate of specific antibodies to the HCV CORE+1 protein in “naive” cronically HCV-infected patients in depends on the stage of liver fibrosis and the HCV subtype by E. V. Lichnaya, M. A. Belopolskaya, V. N. Verbov, A. A. Yakovlev, A. V. Dmitriev, O. V. Kalinina

“…In 53 patients, the fibrosis stage in the liver tissue was evaluated by the TE method using Fibroscan (Echosens, France). …”
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AKR1B1 Expression in the Colorectal Tumor Microenvironment Contributes Towards Its Prognostic Significance by Seçil Demirkol Canlı, Güneş Güner, Aynur Işık, Branka Sosic‐Jurjevic, Aleksandra Djikic Rom, Esin Gülce Seza, Ömer Dizdar, Sandra Dragicevic, Aleksandra Nikolic, Aytekin Akyol, Sreeparna Banerjee

“…Methods We assessed AKR1B1 expression in colorectal tumors utilizing publicly available transcriptomic data from CRC tumors. Single‐cell RNA‐sequencing data from CRC samples were analyzed to determine cell type‐specific expression. …”
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miRNA-503 inhibition exerts anticancer effects and reduces tumor growth in mesothelioma by Miriam Piccioni, Francesco Di Meo, Anna Valentino, Virginia Campani, Maddalena Arigoni, Mirella Tanori, Mariateresa Mancuso, Rossana Cuciniello, Marco Tomasetti, Federica Monaco, Gaia Goteri, Enrico P. Spugnini, Raffaele A. Calogero, Giuseppe De Rosa, Gianfranco Peluso, Alfonso Baldi, Stefania Crispi

“…The effect of miRNA-503 inhibition was evaluated in vitro in mesothelioma cells analysing apoptosis induction and reduction of cancer properties. …”
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A large-scale multi-centre study characterising atrophy heterogeneity in Alzheimer’s disease by Vikram Venkatraghavan, Damiano Archetti, Pierrick Bourgeat, Chenyang Jiang, Mara ten Kate, Anna C. van Loenhoud, Rik Ossenkoppele, Charlotte E. Teunissen, Elsmarieke van de Giessen, Yolande A.L. Pijnenburg, Giovanni B. Frisoni, Béla Weiss, Zoltán Vidnyánszky, Tibor Auer, Stanley Durrleman, Alberto Redolfi, Simon M. Laws, Paul Maruff, Neil P. Oxtoby, Andre Altmann, Daniel C. Alexander, Wiesje M. van der Flier, Frederik Barkhof, Betty M. Tijms

“…This large-scale study evaluates subtype concordance using two data-driven approaches. …”
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Eco-friendly biocontrol of Phytophthora capsici in black pepper (Piper nigrum) using Burkholderia species by Sukamto , K.D. Sutanto, R. Noveriza, S. Hardiyanti, Wiratno , S.R. Djiwanti, D. Manohara, I.B. Rahardjo, R. Harni, W. Amaria, Supriadi

“…Biosafety assays were conducted to evaluate hemolytic activity and hypersensitive response induction. …”
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SQLE-mediated squalene metabolism promotes tumor immune evasion in pancreatic cancer by Junchen Pan, Haixi Liang, Lin Zhou, Wenhua Lu, Bitao Huo, Bitao Huo, Rui Liu, Peng Huang, Peng Huang

“…Flow cytometry analysis and immunohistochemical (IHC) staining of immune cells from the tumor tissues were performed to evaluate changes in immune function. We also employed RNA-sequencing to analyze the gene expression profiles in pancreatic cancer cells (PANC-1) treated with or without squalene. …”
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PCSK9-antibodies fail to block PCSK9-induced inflammation in macrophages and cannot recapitulate protective effects of PCSK9-deficiency in experimental myocardial infarction by Simon Rauterberg, Simon Rauterberg, Carmen Härdtner, Jennifer Hein, Paola Schrepf, Remi Peyronnet, Christoph Koentges, Tamara A. Vico, Carolin Ehlert, Bianca Dufner, Diana Lindner, Constantin von zur Mühlen, Dennis Wolf, Dirk Westermann, Ingo Hilgendorf, Alexander von Ehr

“…Gene expression changes were evaluated using RNA sequencing, and inflammatory responses in bone marrow-derived macrophages (BMDMs) were analyzed in vitro.ResultsPCSK9 was expressed in murine heart tissue at levels comparable to the liver, despite minimal heart RNA expression. …”
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Cancer specific up-regulated lactate genes associated with immunotherapy resistance in a pan-cancer analysis by Shuiting Fu, Jiachen Xu, Chunming Wang, Cheng Zhang, Chengcheng Li, Wenchuan Xie, Guoqiang Wang, Xin Zhu, Yuyan Xu, Yaohong Wen, Jingyuan Pei, Jun Yang, Mingyang Tang, Hongkun Tan, Shangli Cai, Lei Cai, Mingxin Pan

“…The cancer cell-intrinsic mechanism to immune checkpoint inhibitors (ICIs) resistance was measured using single cell sequencing (scRNA-seq) data. Pathway activation was evaluated in the TCGA cohort and CPTAC cohort with transcriptomics and proteomics. …”
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Immunogenicity and safety of CoronaVac vaccine in children and adolescents (Immunita-002, Brazil): A phase IV six-month follow up by Camila Amormino Corsini, Priscila Fernanda da Silva Martins, Priscilla Soares Filgueiras, Adelina Júnia Lourenço, Ana Esther de Souza Lima, Sarah Vieira Contin Gomes, Wander de Jesus Jeremias, Pedro Augusto Alves, Gabriel da Rocha Fernandes, Luciana Lisboa Mota e Castro, Andrea Teixeira-Carvalho, Ana Carolina Campi Azevedo, Caroline De Almeida Leitao Curimbaba, Daniela Aparecida Lorencini, Eolo Morandi Junior, Victor Mattos da Silva, Maria Célia Cervi, Marcos de Carvalho Borges, Maurício Lacerda Nogueira, Guilherme Rodrigues Fernandes Campos, Paulo Roberto Lopes Correa, Taciana Malheiros Lima Carvalho, Jordana Grazziela Alves Coelho dos Reis, Erik Vinícius de Sousa Reis, Leda dos Reis Castilho, Poliana Remundini de Lima, João Paulo Resende do Nascimento, Jaquelline Germano de Oliveira, Olindo Assis Martins Filho, Immunita Team, Rafaella Fortini Queiroz e Grenfell

“…The cellular immune response was evaluated by flow cytometry through the quantification of systemic soluble immune mediators. …”
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