Comparative Effectiveness of Second-Line Targeted Therapies for Metastatic Renal Cell Carcinoma: A Systematic Review and Meta-Analysis of Real-World Observational Studies. by Daniel Y Heng, James Signorovitch, Elyse Swallow, Nanxin Li, Yichen Zhong, Paige Qin, Daisy Y Zhuo, Xufang Wang, Jinhee Park, Sotirios Stergiopoulos, Christian Kollmannsberger

“…<h4>Objective</h4>The optimal sequencing of targeted therapies for metastatic renal cell carcinoma (mRCC) is unknown. …”
Get full text

Pancreatic neuroendocrine tumors and pathogenic variants: a multinational study by Rachel P. Riechelmann, Giovana T. Torrezan, Emily Bergsland, Nitya Raj, Jonathan Strosberg, Farhana Moon, Tiago C. Felismino, Stian Knappskog, Elena Trevizani, Sara Cingarlini, Halfdan Sorbye

“…Germline or somatic variants were evaluated by targeted sequencing of cancer-associated genes. …”
Get full text

Clinical and prognostic significance of m6A hypomethylation and IGF2BP3 overexpression in gastric cancer: an integrated epigenomic-transcriptomic analysis by Xiangchen Hu, Zhe Wang, Youwei Kou, Yujing Huang

“…Methods Methylated RNA immunoprecipitation sequencing (MeRIP-seq) was performed to map m6A epitranscriptomes in paired GC and adjacent normal tissues. …”
Get full text

Detection of possible aminoglycosides resistance mechanisms in Pseudomonas aeruginosa resistant isolates by Maria R. Boushra, Noha A. Hassuna, Gamal F.M. Gad, Reham A. Ibrahem, Nancy G.F.M. Waly

“…Aminoglycoside resistance amplified gene sequences were detected using polymerase chain reaction (PCR). …”
Get full text

Potential role of 4-hydroxyisoleucine in enhancing fertility in male mice with diet-induced obesity by Emmanuel Osei Nkansah, Yunzhu Lan, Hui Zhang, Binbin Xu, Qiaodan Li, Mohammad Ishraq Zafar, Jian Xu

“…ND and HFD controls received saline (0.3 mL/30 g body weight) throughout the intervention period. Comprehensive evaluations included (1) metabolic assessments (body weight, glucose, insulin and pyruvate tolerance tests, and blood serum lipids), (2) sperm analysis (count, concentration, and morphology), (3) fertility testing (mating trials and in vitro fertilization), (4) testicular histopathology (fat deposition and apoptosis), (5) biochemical assays (reproductive hormones, oxidative stress markers, and inflammatory cytokines), and (6) molecular analyses (mRNA sequencing and qPCR validation of differentially expressed genes).Results4-HIL treatment improved metabolic parameters, including reduced weight gain, enhanced glucose tolerance, and optimized blood serum lipids, compared to HFD controls. …”
Get full text

A network Meta-analysis of tripterygium wilfordii polyglycosides tablet,ambrette capsule and RAS blocker in the treatment of IgA nephropathy by FANG Xin-ying, WANG Xiao-qin, JIN Shan-shan, LIN La-mei

“…Objective To evaluate the efficacy and safety of Tripterygium wilfordii tablet(TW),ambrette capsule combined with or without renin-angiotensin system(RAS)blockers in treatment of IgA nephropathy(IgAN)with moderate proteinuria.Methods Cochrane Library,CNKI,WANFANG,Web of Science,China Biology Medicine disc,VIP database and Pubmed were searched with the searching period till August of 2019,to collect randomly controlled trails(RCTs)of Tripterygium wilfordii(TW),ambrette capsule combined with or without renin-angiotensin system(RAS)blockers.Based on Cochrane Systematically Assessment Staff Mannual,the enrolled trials were assessed for their quality.The software RevMan 5.3 was used to plot the risk assessment diagram and perform the network Meta-analysis.With Gemtc 0.14.3 package analysis was performed by Markov Chain Monte Carlo method,with Stata 15 SCURA calculation grade sequencing was performed and a funnel chart was plotted,and the R software was adopted to plot the trace plot and judge model convergence.Results A total of 31 RCTs involving 3192 patients were included.The network Meta-analysis showed that:(1)in the treatment of IgAN in reducing proteinuria,Ambrette capsule+RAS blockers,TW and TW+RAS blockers were superior to RAS blockers(P<0.05).The SMD values(95% CI)were-0.33(-0.54,-0.13),-0.41(-0.72,-0.12),-0.48(-0.74,-0.23),respectively.;(2)Compared to RAS blockers alone,TW+RAS blockers was superior to RAS blockers for reducing serum creatinine(P<0.05),with a SMD(95% CI)of-6.32(-11.06,-1.69);(3)In the risk of adverse events,ambrette capsule+RAS blockers was superior to TW+RAS blockers,with a SMD(95% CI)of 0.19(0.04,0.73).Conclusions Ambrette capsule combined with RAS blockers can effectively reduce urine protein level in IgAN patients with proteinuria,and has a minimal risk of adverse events.TW combined with RAS blockers can reduce serum creatinine to protect patients’ renal functions,with an increased risk of adverse events.…”
Get full text

SPP1 as a Prognostic and Immunotherapeutic Biomarker in Gliomas and Other Cancer Types: A Pan-Cancer Study by Wang K, Wan J, Zheng R, Xiao Y, Lv F, Ge H, Yang G, Cheng Y

“…Functional validation was performed via SPP1 knockdown in glioma cell lines to evaluate effects on proliferation, invasion, and immune interactions.Results: SPP1 was found to be overexpressed in 27 tumor types, with high expression correlating with poor OS, PFS, and increased immune cell infiltration, particularly with CD8+ T cells and macrophages. …”
Get full text

Purslane oleracea polysaccharides alleviate male reproductive damage caused by acrylonitrile by improving gut microbiota dysbiosis and metabolic disorder by Weihua Li, Hongbing Li, Jing Wu, Chenggang Jia, Yanli Lei

“…This study assessed the impact of ACN exposure and evaluated POP intervention on gut microbiota, Metabolic Profiles, and reproductive function. …”
Get full text

The genetic consequences of successful reintroduction of wild turkey (Meleagris gallopavo) in Nebraska by Brittaney L. Buchanan, Magdalena J. Olson, Robert M. Zink, Hernán Vázquez‐Miranda

“…Analysis of 58 birds from Nebraska for mtDNA control region sequences, plus birds from museums and domestic stock, did not recover any groupings consistent with subspecies or our mitogenome results. …”
Get full text

The contribution of integrated and non-integrated pig farms to epidemiological dynamics of porcine reproductive and respiratory syndrome virus in Italy by M. Ustulin, D. Vio, C. Targhetta, C. Zanon, L. Ferino, G. Faustini, G. Franzo

“…Geographical migration patterns and environmental factors influencing viral dispersal were evaluated. Despite being located in the same geographical areas, non-integrated farms exhibited higher viral dispersal speeds, marked by bursts over time. …”
Get full text

Anti-inflammatory actions of ripasudil ameliorate experimental autoimmune uveoretinitis in the acute phase by Tomohito Sato, Masaru Takeuchi, Kozo Harimoto, Hideaki Someya, Masataka Ito, Yoshiaki Nishio

“…This study aimed to evaluate the suppressive effects of ripasudil, a ROCK inhibitor, on ocular inflammation when administered from the onset of EAU and to elucidate the underlying mechanisms of its inhibitory effects.Methods EAU was induced in wild-type C57BL/6 mice by immunisation with IRBP peptide. …”
Get full text

Improved quantification of aortic regurgitation with direct regurgitant jet measurement by four-dimensional flow cardiovascular magnetic resonance in complex congenital heart disea... by Brynn Connor, Makoto Takei, Daniel E. Clark, Shiraz A. Maskatia

“…Methods: In 186 patients with moderate or complex CHD, we evaluated the agreement between different methods of AR quantification by 4DF CMR when compared to volumetry. …”
Get full text

Development of a clinical prediction model for sensitivity to combination therapy of Bcl-2 inhibitors and hypomethylating agents in elderly/unfit patients with acute myeloid leukem... by Yufeng Du, Chunhong Li, Yonghong Chen, Fang Xie, Jinsong Yan

“…The performance of the nomogram was evaluated using a receiver operating characteristic (ROC) curve, calibration plot, and decision curve analysis (DCA). …”
Get full text

Development of a Novel Multiplex PCR Method for the Rapid Detection of SARS-CoV-2, Influenza A Virus, and Influenza B Virus by Liang Ma, Haoyan Zhu, Yongwei Jiang, Xiaomu Kong, Peng Gao, Yi Liu, Meimei Zhao, Guoxiong Deng, Yongtong Cao

“…Based on the genome sequences of SARS-CoV-2, influenza A virus (FluA), and influenza B virus (FluB) with reference to the Chinese Center for Disease Control and Prevention and related literature, specific primers were designed, and a multiplex fluorescent PCR system was established. …”
Get full text

Gut health improvement by locally isolated probiotics and histomorphometric analysis in Wistar rats by Zuhra Bibi, Dilara Abbas Bukhari, Muhammad Qadeer Sarwar, Arifullah, Samina Younas, Tayyab Manzoor, Abdul Rehman

“…Molecular characterization was done through 16S rRNA by using universal primers. After sequencing eight Lactobacillus strains were identified. …”
Get full text

The vaccine-site microenvironment: impacts of antigen, adjuvant, and same-site vaccination on antigen presentation and immune signaling by Craig L Slingluff, Pankaj Kumar, Max O Meneveau, Kevin T Lynch, Sapna P Patel

“…We hypothesized that antigen processing and presentation may occur directly in the VSME, that adding the toll-like receptor 3 (TLR3) agonist polyICLC (pICLC) would enhance markers of immune activation, and that the immune signatures would be enhanced further by repeated vaccination in the same skin site rather than after multiple vaccines in different skin locations.Methods Using RNA sequencing, we evaluated VSME biopsies from patients undergoing subcutaneous/intradermal peptide vaccination against melanoma, with incomplete Freund’s adjuvant (IFA) with or without pICLC. …”
Get full text

Genetic Identification of Brazilian Mammalian Hosts of <i>Trypanosoma cruzi</i>: Improving Blood Meal Source Discrimination in Vector-Borne Transmission by Quezia Moura Oliveira, Thaíla Santos Pessanha, Alena Mayo Iñiguez

“…The results showed that GenBank lacks reference sequences for some native species tested, such as the sylvatic rodent, <i>Necromys lasiurus</i>, and the wild canid, <i>Lycalopex gymnocercus,</i> for <i>cyt</i>b and 12S rDNA, and the rodent <i>Oecomys cleberi</i> for 12S rDNA. …”
Get full text

Development and validation of a prognostic model for predicting survival and immunotherapy benefits in melanoma based on metabolism-relevant genes by Xiaoru Pan, Shiyao Zhou, Ning Mao, Fengming Yao, Yajun Guo, Wenming Zhou, Shengxiu Liu

“…Furthermore, we developed a prognostic model to predict the prognosis of SKCM patients and evaluate the response of SKCM patients to immunotherapy based on the expression of signature genes. …”
Get full text

A non-invasive specimen collection method and a novel simian foamy virus (SFV) DNA quantification assay in New World primates reveal aspects of tissue tropism and improved SFV dete... by Cláudia P Muniz, HaoQiang Zheng, Hongwei Jia, Liliane T F Cavalcante, Anderson M Augusto, Luiz P Fedullo, Alcides Pissinatti, Marcelo A Soares, William M Switzer, André F Santos

“…The qPCR assay was validated using distinct SFV polymerase sequences from seven NWP genera (Callithrix, Sapajus, Saimiri, Ateles, Alouatta, Cacajao and Pithecia). …”
Get full text

The role of fibromodulin in myocardial fibrosis in a diabetic cardiomyopathy rat model by Xiyan Dai, Fan Yang, Dongping Chen, Lu Yang, Zhihui Dong, Can Chen, Jianmin Xiao

“…Subsequently, whole RNA sequencing was conducted on ov‐Fmod RCFs. The gene Col15a1 was evaluated in the DCM rat and all cell models. …”
Get full text