SIU-ICUD: Management of Lymph Node–Positive Prostate Cancer by Haitham Shaheen, Mack Roach, Eman Essam Elsemary

“…<b>Methods</b>: A systematic review was conducted to identify studies evaluating therapeutic strategies for N1M0 prostate cancer. …”
Get full text

Investigation of the Operations of Open Pit Iron Mineralization for Acid Mine Drainage (Adana/Saimbeyli) by Ayşe Dokuzlar, Nil Yapıcı

“…The NP, Net Neutralization Potential (NNP), APP, Neutralization Potential Ratio (NPR=NP/APP) values of the samples were evaluated based on the data obtained. The paragenesis of the mineralization was composed of hematite, gothite siderite and limonite, while constitutes dolomite, calcite and pyrite minerals constitute the host rock. …”
Get full text

Melt analysis of mismatch amplification mutation assays (Melt-MAMA): a functional study of a cost-effective SNP genotyping assay in bacterial models. by Dawn N Birdsell, Talima Pearson, Erin P Price, Heidie M Hornstra, Roxanne D Nera, Nathan Stone, Jeffrey Gruendike, Emily L Kaufman, Amanda H Pettus, Audriana N Hurbon, Jordan L Buchhagen, N Jane Harms, Gvantsa Chanturia, Miklos Gyuranecz, David M Wagner, Paul S Keim

“…We examined the performance of 185 Melt-MAMAs across eight different pathogens using various optimization parameters. We evaluated the effects of genome size and %GC content on assay development. …”
Get full text

Identification of functional rare coding variants in IGF-1 gene in humans with exceptional longevity by Amanat Ali, Zhengdong D. Zhang, Tina Gao, Sandra Aleksic, Evripidis Gavathiotis, Nir Barzilai, Sofiya Milman

“…We performed extended all-atom molecular dynamics simulations to evaluate the impact of Ile91Leu on stability, binding dynamics and energetics of IGF-1 bound to IGF-1R. …”
Get full text

Eyes in the wild: Thelazia callipaeda in wild carnivores of Croatia by Ema Gagović, Šimun Naletilić, Daria Jurković Žilić, Željko Mihaljević, Adnan Hodžić, Relja Beck

“…Considering the zoonotic potential of this parasite, further surveillance and integrated monitoring of vectors and vertebrate hosts are essential for evaluating future risks to public and veterinary health in the region. …”
Get full text

Integrative Proteomics and Genomics Identify Novel Biomarkers and Therapeutic Targets in Vitiligo via Mendelian Randomization by Chenjue Yan, Ling Jiang, Yibo Hu, Ting You, Jing Chen, Songjiang Wu

“…Abstract Introduction Given that the proteome is a major source of therapeutic targets, we conducted a proteome-wide Mendelian randomization (MR) combined with transcriptome sequencing analysis to identify candidate protein markers and therapeutic targets for vitiligo. …”
Get full text

Improvement of Anaerobic Digestion by Addition of Halloysite and Magnetite Powders by Jae Pil Heo, Ilho Bae, Juhee Shin, Myoung-Eun Lee, Younki Lee, Jin Mi Triolo, Seung Gu Shin

“…Additionally, microbial community analysis was performed using 16S rRNA amplicon sequencing, and correlations between the powder concentrations, Gompertz kinetic parameters, and microbial groups were evaluated. …”
Get full text

A novel MRI-based habitat analysis and deep learning for predicting perineural invasion in prostate cancer: a two-center study by Shuitang Deng, Danjiang Huang, Xiaoyu Han, He Zhang, Hui Wang, Guoqun Mao, Weiqun Ao

“…Receiver operating characteristic curve analysis was performed to evaluate the models’ ability to predict PNI. Results The four habitat models exhibited robust performance in predicting PNI, with area under the curve (AUC) values of 0.862–0.935, 0.802–0.957, and 0.859–0.939 in the training, in-vad, and ex-vad cohorts, respectively. …”
Get full text

Phenotypic dichotomy in Crotalus durissus ruruima venom and potential consequences for clinical management of snakebite envenomations. by Mônica Colombini, Anderson M Rocha, Luciana A Freitas-de-Sousa, Alison F A Chaves, Solange M T Serrano, Vinicius C Souza, Vincent L Viala, Inácio L M Junqueira-Azevedo, Felipe A Cerni, Marco A Sartim, Jacqueline A G Sachett, Wuelton M Monteiro, Felipe G Grazziotin, Fan Hui Wen, Manuela B Pucca, Ana M Moura-da-Silva

“…<h4>Methodology/principal findings</h4>This study characterizes the dichotomy of C. d. ruruima venom by analyzing the structure of isoforms differentially expressed in Type I and Type II venoms, assessing their biological activities, and evaluating the implications for snakebite clinical management in Roraima State (northern Brazil). …”
Get full text

Unveiling the therapeutic potential of senescence-related IQGAP2 in pancreatic Cancer through post-GWAS genomic and scRNA-seq analyses by Zilong Bai, Jiale Liang, Yuanhua Nie, Shilong Wang, Dongmin Chang

“…Method We employed Mendelian randomization (MR) alongside single-cell RNA sequencing (scRNA-seq) to explore the involvement of senescence-associated genes (SAGs) in PC. …”
Get full text

An integrative pan-cancer analysis of USP37 and functional validation in pancreatic cancer by Jiafei Chen, Jiafei Chen, Liang Lin, Dongxing Chen, Jingui Wang, Wuhan Zhou

“…Additionally, Western blotting was conducted to evaluate USP37 expression in clinical samples and pancreatic cancer cell lines. …”
Get full text

LINC00601 promotes the progression of glioma via the p-STAT3 signaling pathway by Chao Ma, Linqi Wang, Renwu Zhang, Tong Li, Peng Li, Yuxiang Ding, Dejun Wu, Yinyan Wang

“…Cell proliferation was evaluated using the CCK-8 assay and plate colony formation assay. …”
Get full text

Human induced neural progenitor cells generated from three-dimensional aggregate-based culture significantly improve post-stroke recovery in tMCAO mice by Zeqin Fu, Yue Hu, Yuxia Wang, Zhijie Liu, Mengyuan Li, Yanqiu Guo, Zhiwei Hu, Xingqiang Lai, Junyuan Hu, Yan Liao, Cheguo Cai

“…Methods We aimed to develop a scalable, stable, and efficient 3D aggregate-based method for iNPC production in suspension culture, avoiding detrimental cell dissociation and replating processes. We evaluated the therapeutic potential of iNPCs in the chronic phase of a transient middle cerebral artery occlusion (tMCAO) mouse model and explored iNPC subpopulations via single-cell RNA sequencing to elucidate their pleiotropic therapeutic potentials. …”
Get full text

Treatment outcomes of multi-drug-resistant and rifampicin-resistant tuberculosis with and without isolation of nontuberculous mycobacteria between 2018-2021: A retrospective cohort... by Elizabeth Tabitha Abbew, Roger Laryea, Ama Owusuaa Kwakye, Yaw Adusi Poku, Dorcas Obiri-Yeboah, Lutgarde Lynen, Tom Decroo, Leen Rigouts, Natalie Lorent

“…Mycobacterial smears and cultures were used to monitor treatment response, with further identification of NTM using line probe assays and Sanger sequencing. Multivariable logistic regression models evaluated predictors of NTM isolation and having an unfavourable outcome. …”
Get full text

Biological Control of Streptomyces murinus against Colletotrichum causing Anthracnose Disease on Tomato Fruits by Hien Trang Nguyen, Thanh Long Le, Thy Dan Huyen Nguyen, Thi Thanh Tuyet Truong, Thi Thuy Tien Nguyen

“…This study aimed to identify the Colletotrichum fungus responsible for anthracnose in tomatoes and evaluate the antifungal efficacy of Streptomyces murinus NARZ. …”
Get full text

Retrotransposition-competent L1s are increased in the genomes of individuals with amyotrophic lateral sclerosis by Abigail L. Pfaff, Abigail L. Pfaff, Sulev Kõks, Sulev Kõks

“…Part of this missing heritability may lie in complex variants, such as the long interspersed element 1 (L1) retrotransposon, which have yet to be evaluated. The majority of L1 insertions in the human genome are no longer able to retrotranspose, but to date 279 retrotransposition-competent (RC) L1s have been reported. …”
Get full text

INVESTIGATION OF DICER1 AND BAFF GENE MUTATIONS IN B-CELL NON-HODGKIN LYMPHOMA by Nurcan Çırak, Demet Akdeniz Ödemiş, Hülya Yazıcı

“…In addition, the presence of mutations in the 11th and 25th exons of the DICER1 gene was evaluated by SANGER sequencing. The results of the control and patient groups were analyzed for polymorphism and mutation presence using Chi-square and Fisher tests.Results: The polymorphic regions of c.+3473A&gt;G(rs3742330) in the DICER1 gene and c.-871C&gt;T (rs 9514828) in the BAFF gene were examined in the patient and control groups, but no statistically significant relationship was found. …”
Get full text

Lactobacillus Genus Complex Probiotic-Induced Changes on the Equine Clitoral Microbiome by Fiona Herzog, Kassandra R. Crissman, Kalie F. Beckers, Guoli Zhou, Chin-Chi Liu, Jenny L. Sones

“…Genomic DNA was extracted and used for 16S rRNA sequencing via the Illumina Miseq platform. Abundance was evaluated via Friedman test with pairwise Dunn’s post hoc comparisons. …”
Get full text

Methylation of the Glucocorticoid Receptor Gene in Children with Somatic Symptom Disorder: A Case-Control Study by Kyoko Hatta, Masato Kantake, Kyoko Tanaka, Hirofumi Nakaoka, Toshiaki Shimizu, Hiromichi Shoji

“…<b>Methods:</b> We analyzed NR3C1 exon 1F methylation in cell-free DNA from saliva in 34 children with SSD and 29 age- and sex-matched controls using bisulfite amplicon sequencing. Psychological assessments included the Beck Depression Inventory-II (BDI-II) and KINDL questionnaires to evaluate associations with methylation patterns. …”
Get full text

Exploring non-invasive biomarkers for pulmonary nodule detection based on salivary microbiomics and machine learning algorithms by Chunxia Huang, Qiong Ma, Xiao Zeng, Jiawei He, Fengming You, Xi Fu, Yifeng Ren

“…Using 16 S rRNA gene sequencing, differences in α- and β-diversity and community composition between the group with PN and controls were analyzed. …”
Get full text