SARS-CoV-2 virus in raw wastewater from student residence halls with concomitant 16S rRNA bacterial community structure changes by Ye Li, Kurt T. Ash, Kurt T. Ash, Dominique Joyner, Dominique Joyner, Daniel E. Williams, Terry C. Hazen, Terry C. Hazen, Terry C. Hazen, Terry C. Hazen, Terry C. Hazen

“…This study investigates the impact of the SARS-CoV-2 virus on microbial compositions in raw sewage, utilizing high-throughput 16S rRNA amplicon sequencing to analyze wastewater samples collected from six dormitories over a one-year field trial at the University of Tennessee, Knoxville. …”
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Integrating Microalgal <i>Chlorella</i> Biomass and Biorefinery Residues into Sustainable Agriculture and Food Production: Insights from Lettuce Cultivation by Antira Wichaphian, Apiwit Kamngoen, Wasu Pathom-aree, Wageeporn Maneechote, Tawanchai Khuendee, Yupa Chromkaew, Benjamas Cheirsilp, Douglas J. H. Shyu, Sirasit Srinuanpan

“…Soil incubation experiments further examined nutrient mineralization rates, while DNA sequencing analyzed shifts in rhizosphere microbial communities. …”
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Association between gut microbiota and ultra-processed foods consumption among the patients with type 2 diabetes: a cross-sectional study by Takahiro Ichikawa, Yoshitaka Hashimoto, Yusuke Igarashi, Sayaka Kawai, Ayumi Kaji, Ryosuke Sakai, Takafumi Osaka, Ryo Inoue, Saori Kashiwagi, Katsura Mizushima, Kazuhiko Uchiyama, Tomohisa Takagi, Yuji Naito, Masahide Hamaguchi, Michiaki Fukui

“…Gut microbial composition was evaluated via 16S rRNA gene sequencing. We investigated the association between gut microbiota, previously identified as relevant to T2D, and the density of UPFs intake using Spearman rank correlation coefficients. …”
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An ancient influenza genome from Switzerland allows deeper insights into host adaptation during the 1918 flu pandemic in Europe by Christian Urban, Bram Vrancken, Livia V. Patrono, Ariane Düx, Mathilde Le Vu, Katarina L. Matthes, Nina Maria Burkhard-Koren, Navena Widulin, Thomas Schnalke, Sabina Carraro, Frank Rühli, Philippe Lemey, Kaspar Staub, Sébastien Calvignac-Spencer, Verena J. Schuenemann

“…Results Here, we describe an alternative protocol for efficient ancient RNA sequencing and evaluate its performance on historical samples, including a published positive control. …”
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Oral fungal dysbiosis and systemic immune dysfunction in Chinese patients with schizophrenia by Xia Liu, Zongxin Ling, Yiwen Cheng, Lingbin Wu, Li Shao, Jie Gao, Wenhui Lei, Zhangcheng Zhu, Wenwen Ding, Qinghai Song, Longyou Zhao, Guolin Jin

“…In this cross-sectional study, we enrolled 118 Chinese patients with SZ and 97 age-matched healthy controls (HCs) to evaluate the oral fungal microbiota from tongue coating samples using internal transcribed spacer 1 amplicon sequencing and assess host immunity via multiplex immunoassays. …”
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Analysis of Colorectal Cancer Gene Mutations and Application of Long Blocker Displacement Amplification Technology for High-Throughput Mutation Detection by Ping Lu, Xinglei Su, Sirui Leong, Xuehao Xiu, Ping Song, Junjie Peng, Yunpei Si

“…For 59 CRC tumor samples, LBDA successfully identified KRAS mutations in 37.29% of cases, closely matching results (42.37%) obtained by next-generation sequencing and achieving 88% sensitivity and 100% specificity. …”
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Cross-species RNAi therapy via AAV delivery alleviates neuropathic pain by targeting GCH1 by Heesue Chang, Kyoung Jin Lee, Minkyung Park, Ha-Na Woo, Ji Hyun Kim, Im Kyeung Kang, Hyochan Park, Chan Hee Chon, Heuiran Lee, Hyun Ho Jung

“…We identified universal small-interfering RNA sequences effective across species and developed an AAV-u-shRNA that successfully suppressed GCH1 expression with minimal off-target effects. …”
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Genetic Variability of Loci Affecting Meat Quality and Production in Nero Siciliano Pig Breed by Serena Tumino, Morena Carlentini, Giorgio Chessari, Andrea Criscione, Aurora Antoci, Donata Marletta, Salvatore Bordonaro

“…Genotypes have been determined using PCR-RFLP and Sanger sequencing. Alleles linked to different growth rates and back fat deposition showed high frequencies (<i>MC4R</i> c.175C—0.93; <i>LEP</i> g.3469T—0.91) in the whole sample. …”
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d-Histidine inhibits Streptococcus mutans growth as a potential anti-caries agent by Liuchang Yang, Yuxing Chen, Yaqi Chi, Xiaolin Chen, Yiran Zhao, Mingrui Zhang, Xuemeng Wang, Yongliang Li, Jie Nie, Xiaoyan Wang

“…Anti-caries efficacy was evaluated in a rat model using Micro-CT and Keyes scoring.Results D-His significantly inhibits the planktonic growth of S. mutans and delays biofilm formation, particularly in the early stages. …”
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Lactiplantibacillus plantarum YY‐112 ameliorates mouse immunosuppression by enhancing B/T‐cell activation and maintaining Th1/Th2 homeostasis by Ying Yang, Mengfan Luo, Wanyi Zhou, Wenyang Tao, Jingrui Li, Jianrong Xing, Quanqin Tan, Yuxing Guo

“…Abstract In recent years, the development of immunosuppression has become common, and probiotics are a vital immunomodulatory strategy. This study evaluated the alleviating effect of Lactiplantibacillus plantarum (L. plantarum) YY‐112 on cyclophosphamide‐induced immune imbalance and resolved the possible mechanism of action. …”
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Recurrent rhabdomyolysis caused by palmitoyltransferase II (CPT-2) deficiency but complete normal acylcarnitine profile: A patient presentation and review of the literature by Chih-Hsuan Lu, Chia-Feng Yang, Yun-Ru Chen, Yann-Jang Chen, Yung-Hsiu Lu, Dau-Ming Niu

“…The results of our study offer crucial insights into evaluating the severity associated with individual genetic variants. …”
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Cystatin B Promotes the Proliferation, Migration, and Invasion of Intrahepatic Cholangiocarcinoma by Dai Zhang, Bao-Ye Sun, Jing-Fang Wu, Zhu-Tao Wang, Su-Su Zheng, Guo-Qiang Sun, Xu-Kang Gao, Jian Zhou, Jia Fan, Bo Hu, Shuang-Jian Qiu, Bo-Heng Zhang

“…<b>Methods</b>: By analyzing transcriptome sequencing data from the FU-iCCA cohort, the iCCA-27 cohort, and three public databases, we identified genes associated with iCCA prognosis and selected CSTB as the subject of our study. …”
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Bacterial community shifts in Fusarium-induced avocado root rot and the antagonistic potential of Bacillus siamensis NB92 by Chengxian Wang, Chengxian Wang, Chengxian Wang, Zhijiao Song, Zhijiao Song, Zhijiao Song, Xue Li, Xue Li, Xue Li, Qing Liu, Qing Liu, Qing Liu

“…Here, we combined culture-independent 16S rDNA amplicon sequencing with culture-dependent isolation to characterize bacterial communities in healthy and Fusarium-infested avocado bulk and rhizosphere soils. …”
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TRIM26 exacerbates pathological cardiac hypertrophy by activating TAK1 by Xiaochuang Xia, Huajing Shan, Zhaoxia Jin, Tengfei Ma, Yemao Liu, Jianqing Zhang, Han Tian, Bizhen Dong, Chengsheng Xu, Shaoze Chen

“…In PE-treated NRCMs, Trim26 overexpression promoted cardiomyocyte enlargement and inflammation, while Trim26 knockdown had the opposite effects. RNA sequencing and molecular biology methodologies were performed to identify targets conducive to TRIM26 function. …”
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Taming Mambas for 3D Medical Image Segmentation by Luca Lumetti, Vittorio Pipoli, Kevin Marchesini, Elisa Ficarra, Costantino Grana, Federico Bolelli

“…In this paper, we evaluate the effectiveness of Mamba-based architectures in comparison to state-of-the-art convolutional and Transformer-based models for 3D medical image segmentation across three well-established datasets: Synapse Abdomen, MSD BrainTumor, and ACDC. …”
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Targeting the PTN/PTPRZ1-ROS Pathway to Promote Bone Regeneration by Kai Zhao, Yusi Guo, Ying He, Yujia Wu, Zhewen Hu, Xiaopei Chi, Xuliang Deng

“…<b>Methods</b>: In this study, we explored the effects and mechanisms of PTN in regulating osteogenic functions using real-time quantitative PCR, immunofluorescence, ALP detection, a TUNEL assay, RNA sequencing, and phosphorylation quantitative proteomics. …”
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Virus–Host Interactions and Genetic Exchange in Mixed Infections of Tomato Yellow Leaf Curl Virus (TYLCV), Tomato Leaf Curl New Delhi Virus (ToLCNDV), and Tomato Chlorosis Virus (T... by Isabel M. Fortes, Luis Díaz-Martínez, Enrique Moriones, Ana Grande-Pérez

“…Genetic exchange was detected in defective DNA (def-DNA) molecules using high-throughput sequencing (HTS), indicating potential genetic interactions between these viruses. …”
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Bacteriome Signature in SARS-CoV-2-Infected Patients Correlates with Increased Gut Permeability and Systemic Inflammatory Cytokines by Larissa S. Souza, Alexandre S. Ferreira-Junior, Pedro C. Estella, Ricardo K. Noda, Lhorena F. Sousa, Miguel T. Y. Murata, Lucas A. L. Carvalho, João L. Brisotti, Daniel G. Pinheiro, Josias Rodrigues, Carlos M. C. B. Fortaleza, Gislane L. V. de Oliveira

“…In this study, we assessed the gut bacteriome and permeability in SARS-CoV-2-infected patients using 16S sequencing and ELISA assays, respectively. We also measured blood inflammatory cytokines and fecal secretory IgA to evaluate systemic and mucosal immune responses. …”
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Peripheral Blood Exosomal miR-184-3p in Methamphetamine Use Disorder: Biomarker Potential and CRTC1-Mediated Neuroadaptation by Yan Zhao, Zhuoming Zhao, Qianqian Sun, Hang Su, Tianzhen Chen, Xiaomin Xu, Xiaotong Li, Sai Shi, Jiang Du, Haifeng Jiang, Min Zhao

“…Subsequently, the diagnostic ability of these two miRNAs for MUD was evaluated using ROC analysis. Finally, the regulatory relationship between miRNA-184-3p and its downstream target gene CRTC1 was verified by dual luciferase reporter assay. …”
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Impact of bile duct stenting on the management of symptomatic choledocholithiasis: a retrospective multicenter analysis by Artur Rebelo, Marie L. Tischer, Jonas Rosendahl, Jens Walldorf, Tawfik Mosa, Jörg Kleeff, Johannes Klose

“…These associations may reflect both clinical severity at baseline and procedural sequencing. A more restrictive placement of biliary stents might be advisable.…”
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