Mannose-B from Codonopsis pilosula modulates LAMB1 expression to enhance trophoblast function and alleviate subchorionic hematoma by Qian Sun, Yuan Gao, Kadirya Asan, Jing Ji, Jinjin Xu, Xiaoyan Wu, Jie Ren, Wen Feng, Mengwei Song, Sen Wang, Boyu Zhang, Ying Zhou, Conghui Han, Shun Liu, Wen Yang

“…A SCH rat model was established to evaluate changes in coagulation parameters, litter size, fetal viability, and fetal and placental weights. …”
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A Novel Synonymous Variant in SQSTM1 Causes Neurodegeneration With Ataxia, Dystonia, and Gaze Palsy Revealed by Urine‐Derived Cells‐Based Functional Analysis by Shinji Masuko, Mitsuto Sato, Katsuya Nakamura, Kohei Hamanaka, Satoko Miyatake, Yuji Inaba, Tomoki Kosho, Naomichi Matsumoto, Yoshiki Sekijima

“…Methods We performed clinical and genetic laboratory evaluations of the two patients and their healthy parents. …”
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Short-Term Evolutionary Features and Circadian Clock-Modulated Gene Expression Analysis of <i>Piezo</i>, <i>nanchung</i>, and <i>αTubulin at 67C</i> in a Romanian Population of <i>... by Adriana-Sebastiana Musca, Attila Cristian Ratiu, Adrian Ionascu, Nicoleta-Denisa Constantin, Marius Zahan

“…Our results imply that short-term evolutionary accumulated single nucleotide and indel variants are overrepresented within introns, a propensity evaluated through the mutation accumulation tendency (MAT) original parameter. …”
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Cancer-associated fibroblasts gene signature: a novel approach to survival prediction and immunotherapy guidance in colon cancer by Wenbing Zhang, Chi Yang, Ye Lu, Ye Lu, Chenling Tang, Mengyu Zhao, Zhaohui Wang, Jidong Gao, Jidong Gao, Shuangjiu Hu, Zhihua Chen

“…Additionally, the impact of these risk categories on the tumor microenvironment was evaluated. The ability of CAFGs signature to assess prognosis and guide treatment was validated using external cohorts. …”
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Aurora kinase B inhibitor AZD1152: repurposing for treatment of lupus nephritis driven by the results of clinical trialsResearch in context by Yue Zhao, Zuguo Zheng, Xuexiao Jin, Shaoshan Liang, Changming Zhang, Mingchao Zhang, Yue Lang, Ping Li, Zhihong Liu

“…The therapeutic efficacy of AZD1152 was evaluated in MRL/lpr mice. Transcriptome sequencing and functional assays were performed to elucidate its mechanisms of action. …”
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A Randomized Crossover Trial Assessing Plaque Regrowth Dynamics in Adults With Use of an Oscillating‐Rotating Electric Toothbrush Versus a Manual Toothbrush Measured by Digital Pla... by Priscila Ferrari Peron, Ralf Adam, Julie Grender, Uta Mesples, Phyllis Hoke, Mike Rubush, Mary Kay Anastasia, Christina Erbe

“…Material and Methods Thirty‐four participants were randomized to one of 4 treatment sequences, each having four 8‐day treatment periods. …”
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WFDC3 identified as a prognostic and immune biomarker in pancreatic cancer by Bohan Liu, Xuqing Shi, Tianqi Liu, Huanwen Wu, Zhiyong Liang

“…Bulk and single-cell RNA sequencing datasets from the TCGA and GEO repositories were analyzed to assess WFDC3 expression in PAAD tissues. …”
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Therapeutic Potential of Emricasan, a Pan-Caspase Inhibitor, in Reducing Cell Death and Extracellular Matrix Accumulation in Fuchs Endothelial Corneal Dystrophy by Sohya Fujimoto, Mako Endo, Shigehito Tonomura, Fuuga Tsuji, Hirotaka Haraguchi, Kanna Hasegawa, Taisuke Numao, Ayaka Izumi, Theofilos Tourtas, Ursula Schlötzer-Schrehardt, Friedrich Kruse, Yuki Oyama, Masahito Ikawa, Albert S. Jun, Noriko Koizumi, Naoki Okumura

“…Endothelial cell density, hexagonality, cell size variation, and guttae area were evaluated by contact specular microscopy, while transcriptomic changes were analyzed via RNA sequencing. …”
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Inhibition of stromal MAOA leading activation of WNT5A enhance prostate cancer immunotherapy by involving the transition of cancer-associated fibroblasts by Hui Li, Mengyao Li, Lijun Yang, Tong Lu, Zhite Zhao, Yaohua Hu, Xinglin He, Yifan Ma, Minli Huang, Changhong Shi

“…However, the detailed mechanisms of MAOA’s interaction with TIME have not been fully elucidated.Methods We reanalyzed a single-cell sequencing dataset to evaluate the role of MAOA in the stroma, verify the impact of stromal MAOA alterations on CD8+ T cell responses by co-culturing stromal cells and immune cells in vitro. …”
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Abdominal ultrasound stimulation alleviates DSS-induced colitis and behavioral disorders in mice by mediating the microbiota–gut–brain axis balance by Cong-Yong Gao, Yi-Ju Pan, Wei-Shen Su, Chun-Yi Wu, Ting-Yu Chang, Feng-Yi Yang

“…Mouse biological samples were analyzed, and behavior was evaluated. [18F]FEPPA PET/CT imaging was employed to track and quantify inflammation in the abdomen and brain. …”
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Biochemical analysis of the Caspian Sea sediments: Implications for environmental pollution and bioremediation by Aigul Mamataeva, Askhat Kuatbayev, Kristina Gartsiyanov, Umirzokov Azamat Abdurashidovich, Abdiyeva Flora, Makhmudova Gulrukh Ravshanbekovna, Tulakov Ulugbek Toshmamatovich, Bobojonov Otabek Khakimboy ugli, I.B. Sapaev, Dilshodbek Mamasoatov, Bilolova Zamira Bakhtiyarovna, Eshpulatov Nodir Mamatqurbonovich, Mansur Kholmirzaev

“…This study aimed to assess pollution levels in the Caspian Sea sediments and evaluate the potential for microbial bioremediation. …”
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Functionally deficient UBOX5 variants and primary angle-closure glaucoma by Zheng Li, Wee Ling Chng, Zhehao Liu, Tan Do, Masakazu Nakano, Li Jia Chen, Yunhua Loo, Anita S. Y. Chan, Fotis Topouzis, Monisha E. Nongpiur, Mineo Ozaki, Satoko Nakano, Toshiaki Kubota, Shamira A. Perera, Rahat Husain, Tina T. L. Wong, Ching-Yu Cheng, Ching Lin Ho, Khaled Abu-Amero, Hon-Tym Wong, Mônica Barbosa de Melo, Nguyen Do Thi Ngoc Hien, Nguyen Van Trinh, Nguyen Thi Thanh Huong, Yaakub Azhany, Rodolfo Perez-Grossmann, Poemen PM Chan, Kelsey V. Stuart, Mahantesh I. Biradar, Anita Szabo, Eleftherios Anastasopoulos, Dimitrios A. Giannoulis, Panagiota Ntonti, Evangelia Papakonstantinou, Alexandros Lambropoulos, Anthoula Chatzikyriakidou, Vassilis Kilintzis, Humaira Ayub, Shazia Micheal, Yee Yee Aung, Edgar U. Leuenberger, Antonio Fea, Naing Naing Mon, Amihan Anajao, Xuezhi Bi, Yee Jiun Kok, Rachel S. Chong, Pui-Yi Boey, Darrell Zi Jing Tan, Wendy Wan Ling Sin, Balram Chowbay, Chaw Chaw Khaing, Yin Mon Aung, Rigo Daniel Reyes, Evangelia S. Panagiotou, Dimitrios G. Mikropoulos, Irini C. Voudouragkaki, Georgios D. Panos, Zhicheng Xie, Xiao Yin Chen, Yi Ting Lim, Wee Yang Meah, Ying Shi Lee, Candice Ee Hua Ho, Pearlyn Mei Xin Yeo, Yoko Ikeda, Yuichi Tokuda, Masami Tanaka, Natsue Omi, Morio Ueno, José P. C. de Vasconcellos, Vital P. Costa, Ricardo Y. Abe, Bruno B. de Souza, Guillermo B. Fong, Vania V. Castro, Ricardo Fujita, Maria L. Guevara-Fujita, Farah Akhtar, Mahmood Ali, Mary Ann T. Catacutan, Irene R. Felarca, Chona S. Liao, Carlo Lavia, Hlaing May Than, Khin Thida Oo, Phyu P. Soe-Kyaw, Paolo Frezzotti, Francesca Pasutto, Raquel Quino, Zaw Minn-Din, Nay Lin Oo, Laura Dallorto, Saw Htoo Set, Vi Huyen Doan, Raheel Qamar, Jamil Miguel Neto, Saleh Al-Obeidan, Clement C. Tham, Kazuhiko Mori, Chie Sotozono, Shigeru Kinoshita, Anastasios G. Konstas, Ahmad Tajudin Liza-Sharmini, Juan C. Zenteno, Nhu Hon Do, Paul J. Foster, Kei Tashiro, Chi Pui Pang, Anthony P. Khawaja, Tin Aung, Zhenxun Wang, Chiea Chuen Khor

“…Through whole exome sequencing, we analysed the association between gene-based burden of rare, protein-altering genetic variants and disease risk in 4,667 affected individuals and 5,473 unaffected controls. …”
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TGFBR3 inhibits progression of papillary thyroid cancer by inhibiting the PI3K/AKT pathway and EMT by Hanrong Zhang, Junxin Chen, Xin Chen, Chuimian Zeng, Pengyuan Zhang, Jiewen Jin, Haipeng Xiao, Yanbing Li, Hongyu Guan, Hai Li

“…Edu, wound healing, and Transwell assays were used to evaluate cell proliferation, migration, and invasion. …”
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Bartonella spp. and hematological changes in privately owned domestic cats from Rio de Janeiro, Brazil by Aline Moreira Souza, Nadia Regina Pereira Almosny, Alexsandra Rodrigues Mendonça Favacho, Daniele Nunes Pereira Almeida, Renata Fernandes Ferreira, Eliane Oliveira Ferreira, Namir Santos Moreira, Elba Regina Sampaio Lemos

“…Results: A total of 74.85% (122/163) of the tested cats were positive for Bartonella spp and partial sequencing confirmed to be B. henselae. All hematological findings from the 163 cats tested (PCR-positive and negative), presented normal limits. …”
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Integration of Transcriptomic and Single-Cell Data to Uncover Senescence- and Ferroptosis-Associated Biomarkers in Sepsis by Xiangqian Zhang, Yiran Zhou, Hang Li, Mengru Chen, Fang Peng, Ning Li

“…Receiver operating characteristic (ROC) analysis was performed to evaluate their diagnostic potential, while single-cell RNA sequencing (scRNA-seq) was used to assess their immune-cell-specific expression. …”
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Validating Multicenter Cohort Circular RNA Model for Early Screening and Diagnosis of Gestational Diabetes Mellitus by Shuo Ma, Yaya Chen, Zhexi Gu, Jiwei Wang, Fengfeng Zhao, Yuming Yao, Gulinaizhaer Abudushalamu, Shijie Cai, Xiaobo Fan, Miao Miao, Xun Gao, Chen Zhang, Guoqiu Wu

“…Methods High-throughput sequencing was employed to screen for key circular RNAs (circRNAs), which were then evaluated using reverse transcription quantitative polymerase chain reaction. …”
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COI barcoding can distinguish bisexual and parthenogenetic populations of Haemaphysalis longicornis in Japan: Revisiting methods with SNP analysis as another possible method by Mizue Inumaru, Kentaro Itokawa, Ryo Matsumura, Kyoko Sawabe, Mamoru Watanabe, Haruhiko Isawa, Shinji Kasai, Yukiko Higa

“…The DNA was used for COI barcoding by direct sequencing and genotyping SNPs in the nuclear genome. …”
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High-density Linkage Mapping and Identification of Quantitative Trait Loci Associated with Scab Resistance in Pecan by Gaurab Bhattarai, Shanshan Cao, Nolan Bentley, Patricia Klein, Clive H. Bock, Cristina Pisani, Patrick J. Conner

“…Molecular markers were developed using genotyping by sequencing, and linkage maps were constructed for each parent following the two-way pseudo-test-cross strategy used for cross-pollinated species. …”
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Clinical and functional characterization of a novel STUB1 mutation in a Chinese spinocerebellar ataxia 48 pedigree by Jiaqi Li, Wenyi Xie, Jian-Min Chen, Chun-Zuan Xu, Ya-Li Huang, Sheng Chen, Chang-Yun Liu, Ying-Qian Lu, Zhang-Yu Zou

“…Y252S) through whole-exome sequencing. The variant was interpreted as a variant of uncertain significance, so we conducted a series of experiments using minigene plasmids to evaluate the pathogenicity of the variant. …”
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Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy by Shimiao Bu, Shimiao Bu, Jiang-Yue Ling, Jiang-Yue Ling, Xiaojun Wu, Xiaojun Wu, Liting Zhang, Liting Zhang, Xiangyu Shi, Lang Huang, Zheng Zhao, Ying Yang, Zongqin Xiang, Yong U. Liu, Yong U. Liu, Yufeng Liu, Yuehong Zhang, Yuehong Zhang

“…We analyzed peripheral blood mononuclear cells (PBMCs) using RNA sequencing and quantitative PCR (qPCR) to pinpoint differentially expressed genes (DEGs). …”
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