Prenatal tobacco smoke exposure is associated with childhood DNA CpG methylation. by Carrie V Breton, Kimberly D Siegmund, Bonnie R Joubert, Xinhui Wang, Weiliang Qui, Vincent Carey, Wenche Nystad, Siri E Håberg, Carole Ober, Dan Nicolae, Kathleen C Barnes, Fernando Martinez, Andy Liu, Robert Lemanske, Robert Strunk, Scott Weiss, Stephanie London, Frank Gilliland, Benjamin Raby, Asthma BRIDGE consortium

“…<h4>Results</h4>Of 27,578 loci evaluated, 22,131 (80%) passed quality control assessment and were analyzed. …”
Get full text

Oral Administration of Faecalibacterium prausnitzii Decreased the Incidence of Severe Diarrhea and Related Mortality Rate and Increased Weight Gain in Preweaned Dairy Heifers. by Carla Foditsch, Richard Van Vleck Pereira, Erika Korzune Ganda, Marilia Souza Gomez, Eduardo Carvalho Marques, Thiago Santin, Rodrigo Carvalho Bicalho

“…The objective of this study was to evaluate the effects of administering a live culture of Faecalibacterium prausnitzii to newborn dairy calves on subsequent growth, health, and fecal microbiome. …”
Get full text

ORF1p promotes proliferation and invasion of esophageal squamous cell carcinoma cells by regulating AJUBA expression by YANG Fan, LI Jiangyang, DAI Xiaoyan

“…Methods ① Western blotting was performed to compare the expression of ORF1p between normal esophageal squamous epithelial cells and ESCC cells. ② Immunohistochemistry (IHC) assay was used to examine ORF1p expression in ESCC tissues and paired normal tissues adjacent to tumor. ③ The effects of ORF1p knockdown and overexpression on malignant behaviors in ESCC cells were determined through functional assays. ④ Xenograft tumor model in nude mice was established to evaluate the impact of ORF1p on tumor growth in vivo. ⑤ Transcriptome sequencing combined with cell functional rescue experiments were conducted to identify downstream targets regulated by ORF1p. …”
Get full text

HLA-A∗03:01 as predictive genetic biomarker for glatiramer acetate treatment response in multiple sclerosis: a retrospective cohort analysisResearch in context by Brian C. Zhang, Tilman Schneider-Hohendorf, Rebecca Elyanow, Beatrice Pignolet, Simon Falk, Christian Wünsch, Marie Deffner, Erik Yusko, Damon May, Daniel Mattox, Eva Dawin, Lisa Ann Gerdes, Florence Bucciarelli, Lisa Revie, Gisela Antony, Sven Jarius, Christiane Seidel, Makbule Senel, Stefan Bittner, Felix Luessi, Joachim Havla, Matthias Knop, Manuel A. Friese, Susanne Rothacher, Anke Salmen, Fumie Hayashi, Roland Henry, Stacy Caillier, Adam Santaniello, Maria Seipelt, Christoph Heesen, Sandra Nischwitz, Antonios Bayas, Hayrettin Tumani, Florian Then Bergh, Gerd Meyer zu Hörste, Tania Kümpfel, Catharina C. Gross, Brigitte Wildemann, Martin Kerschensteiner, Ralf Gold, Sven G. Meuth, Frauke Zipp, Bruce A.C. Cree, Jorge Oksenberg, Michael R. Wilson, Stephen L. Hauser, Scott S. Zamvil, Luisa Klotz, Roland Liblau, Harlan Robins, Joseph J. Sabatino, Jr., Heinz Wiendl, Nicholas Schwab, Jessa Alexander, Riley Bove, Sergio Baranzini, Bruce A.C. Cree, Eduardo Caverzasi, Richard Cuneo, Stacy J. Caillier, Tiffany Cooper, Ari J. Green, Chu-Yueh Guo, Jeffrey M. Gelfand, Refujia Gomez, Sasha Gupta, Jill Hollenbach, Meagan Harms, Roland G. Henry, Stephen L. Hauser, Myra Mendoza, Jorge R. Oksenberg, Nico Papinutto, Sam Pleasure, Adam Santaniello, Joseph J. Sabatino, Jr., William A. Stern, Michael R. Wilson, Scott Zamvil, Orhan Aktas, Antje Giede-Jeppe, Barbara Gisevius, Markus Kowarik, Friedemann Paul, Veit Rothhammer, Corinna Trebst, Uwe Zettl

“…Both sets of common sequences clonally expanded after GA treatment in a first validation cohort and predicted GA exposure in two further validation cohorts. …”
Get full text

Patterns of inflammation and immune activation by coreceptor use in people living with HIV-1 by Francisco Xavier Guerra-Castillo, Francisco Xavier Guerra-Castillo, Sandra Pinto-Cardoso, Santiago Ávila-Ríos, Monserrat Chávez-Torres, Amy Peralta-Prado, Carolina González-Torres, Javier Gaytán-Cervantes, Brenda Requena-Benitez, Dafne Díaz-Rivera, Carmen Alaez-Verson, María Concepción Hernández-García, Vilma Carolina Bekker-Méndez

“…Logistic regression was used to evaluate associations between immune activation profiles and the presence of X4-tropic viruses.ResultsNinety-eight individuals had high-quality V3 loop sequences, 81.6% harbored only R5 variants (R5 group), while 18.4% had mixed R5/X4 populations (X4 group). …”
Get full text

Hepatitis B virus mutation pattern rtL180M+A181C+M204V may contribute to entecavir resistance in clinical practice by Yan Liu, Yi Zhou, Xiaodong Li, Ming Niu, Rongjuan Chen, Jinman Shao, Lanlan Si, Dan Luo, Yayun Lin, Le Li, Kai Zhang, Xiaohe Xiao, Zhihui Xu, Min Liu, Mengji Lu, Fabien Zoulim, Dongping Xu

“…HBV reverse transcriptase (RT) gene was screened by direct sequencing and verified by clonal sequencing. Phenotypic analysis was performed for evaluating replication capacity and drug susceptibility.Results: Classical ETV-resistance mutations of HBV were detected in 1252 patients who were receiving ETV therapy. …”
Get full text

Response‐adapted zanubrutinib and tislelizumab as a potential strategy to enhance CD19 CAR T‐cell therapy in relapsed/refractory large B‐cell lymphoma: A retrospective observationa... by Rong Shen, Wei‐Guo Cao, Li Wang, Ling‐Shuang Sheng, Yi‐Lun Zhang, Wen Wu, Peng‐Peng Xu, Shu Cheng, Meng‐Ke Liu, Yan Dong, Yue Wang, Xiang‐Qin Weng, Xu‐Feng Jiang, Qi Song, Hong‐Mei Yi, Lei Li, Sheng Chen, Zi‐Xun Yan, Wei‐Li Zhao

“…DNA sequencing and RNA sequencing were performed on available tumour samples to analyse genetic aberrations and TME characteristics. …”
Get full text

463 Identification of molecular and cellular events during recurrence of focal segmental glomerulosclerosis in human allografts by Haseeb Zubair, Thomas V Rousselle, Amol C Shetty, Azim Shafquat, Akalin Enver, Manish Talwar, Daniel G Maluf, Gallon Lorenzo, Valeria R Mas

“…Methods/Study Population: In this study, we evaluate the molecular and cellular events associated with primary FSGS in both native and transplant kidneys. …”
Get full text

L-methionine promotes CD8+ T cells killing hepatocellular carcinoma by inhibiting NR1I2/PCSK9 signaling by Chengsha Yuan, Changpeng Hu, Huyue Zhou, Wuyi Liu, Wenjing Lai, Yafeng Liu, Yue Yin, Guobing Li, Rong Zhang

“…Co-culture experiments were performed to evaluate the impact of L-methionine on CD8+ T cell-mediated killing of liver cancer cells. …”
Get full text

Genomic and molecular characterization of a ceftazidime-avibactam resistant Klebsiella pneumoniae strain isolated from a Chinese tertiary hospital by Yanye Tu, Hui Gao, Feng Wang, Rongqing Zhao, Shiyong Chen, Yanzi Chang, Hong Li

“…Experiments on conjugation were conducted to evaluate the transmissibility of the resistance plasmids. …”
Get full text

Knockout of bcas3 gene causes neurodevelopment defects in zebrafish by Huihui Liu, Nianyi Sun, Zhenxing Liu, Jinze Li, Xianqin Zhang

“…Transcriptome sequencing analysis was used to identify dysregulated pathways associated with development process. …”
Get full text

A novel PAK1/TCF1 regulatory axis promotes non-small cell lung cancer progression by Chuangang Lu, Yuncong Su, Youzhong Xu, Siyuan Sheng, Taiting Chen, Juan Li

“…Mechanistically, western blot (WB) and immunoprecipitation analysis evaluated the interaction between PAK1 and TCF1 in NSCLC. …”
Get full text

Post-sepsis chronic muscle weakness can be prevented by pharmacological protection of mitochondria by Meagan S. Kingren, Alexander R. Keeble, Alyson M. Galvan-Lara, Jodi M. Ogle, Zoltán Ungvári, Daret K. St Clair, Timothy A. Butterfield, Allison M. Owen, Christopher S. Fry, Samir P. Patel, Hiroshi Saito

“…Following sepsis development in C57BL/6 mice, we evaluated the effect of the mitochondria-targeting synthetic tetrapeptide SS-31 in protecting mitochondria from sepsis-induced damage and preventing skeletal muscle weakness development. …”
Get full text

Synthesis and the impact of hydroxyapatite nanoparticles on the viability and activity of rhizobacteria by Bedah Rupaedah, Indrika Novella, Atiek Rostika Noviyanti, Diana Rakhmawaty Eddy, Anna Safarrida, Abdul Hapid, Zhafira Amila Haqqa, Suryana Suryana, Irwan Kurnia, Fathiyah Inayatirrahmi

“…Rhizobacteria were encapsulated within the carrier material, and their viability was evaluated using the total plate count method. Following their immobilization on nHA, the phosphate-solubilizing capacity of rhizobacteria was evaluated using Pikovskaya’s medium. …”
Get full text

Direct and indirect immune effects of CMP-001, a virus-like particle containing a TLR9 agonist by George J Weiner, Aliasger K Salem, Shakoora A Sabree, Andrew P Voigt, Sue E Blackwell, Ajaykumar Vishwakarma, Michael S Chimenti

“…Here, we explore mechanisms by which the initial effects of CMP-001 on pDCs activate other cells that can contribute to development of an antitumor T-cell response.Methods Uptake of CMP-001 by various peripheral blood mononuclear cell (PBMC) populations and response to anti-Qβ-coated CMP-001 were evaluated by flow cytometry and single-cell RNA sequencing. …”
Get full text

Platelets in preeclampsia: an observational study of indices associated with aspirin nonresponsiveness, activation and transcriptional landscape by Gashaw Garedew Woldeamanuel, Kenean Getaneh Tlaye, Xueqin Wang, Long Nguyen-Hoang, Qiongjie Zhou, Yinan Wang, Bo Wah Leung, Yao Wang, Liona C. Poon, Chi Chiu Wang

“…RNA sequencing (RNA-seq) was performed to investigate the transcriptomic profile of platelets. …”
Get full text

Salt Stress Leads to Morphological and Transcriptional Changes in Roots of Pumpkins (<i>Cucurbita</i> spp.) by Hongjiu Liu, Ding Ding, Yeshuo Sun, Ruiping Ma, Xiaoqing Yang, Jie Liu, Guoxin Zhang

“…To this end, this study evaluated the effects of salinity stress on root architecture in <i>C</i>. …”
Get full text

Assessing the impact of oscillating dietary crude protein on the stability of the rumen microbiome in dairy cattle by F. L. Viquez-Umana, M. G. Erickson, J. D. Young, G. I. Zanton, M. A. Wattiaux, G. Suen, H. C. Mantovani

“…Rumen samples were collected 4 h post-feeding, and amplicon libraries of the V4 region of the 16S rRNA gene were sequenced and analyzed to assess changes in microbiome composition. …”
Get full text

Growth differentiation factor 15 aggravates sepsis-induced cognitive and memory impairments by promoting microglial inflammatory responses and phagocytosis by Lijiao Chen, Shiyuan Luo, Ting Liu, Zhewei Shuai, Yifan Song, Qianzi Yang, Ying Wang, Hongjun Huang, Yan Luo

“…Microglial activation and phagocytosis were evaluated using immunofluorescence and Golgi staining. …”
Get full text

Single-cell ligand–receptor profiling reveals an immunotherapy-responsive subtype and prognostic signature in triple-negative breast cancer by Chuanzhi Chen, Jiahui Qi, Weichao Lin, Chunlan Fu, Xin Jin

“…From this dataset, we identified LR pairs that exhibited significant intercellular crosstalk and evaluated their prognostic relevance in a METABRIC TNBC cohort (n = 298). …”
Get full text