Genetic Diversity and Population Size Change of Balanites aegyptiaca (L.) Delile Natural Populations by Lucky Dartsa Wakawa, Adejoke Olukemi Akinyele

“…This study aimed to evaluate the diversity of Balanites aegyptiaca within its native Nigerian populations to identify suitable sources for its domestication and conservation. …”
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Primary Cells from a <i>CD46</i>-Edited Bovine Heifer Have Reduced BVDV Susceptibility Despite Viral Adaptation to Heparan Sulfate by Alexandria C. Krueger, Brian L. Vander Ley, Michael P. Heaton, Tad S. Sonstegard, Aspen M. Workman

“…Serial passage of BVDV on <i>CD46</i>-edited MDBK cells selected for virus variants capable of CD46-independent infection. Virus genome sequencing revealed mutations in the viral E^RNS gene predicted to enhance HS-mediated entry. …”
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Identification of potential insect ecological interactions using a metabarcoding approach by Nicole D. Borsato, Katherine Lunn, Nina R. Garrett, Alejandro José Biganzoli-Rangel, Daniel Marquina, Dirk Steinke, Robin Floyd, Elizabeth L. Clare

“…The decreasing costs of sequencing makes the mass analysis of thousands of target species possible. …”
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Assessment and selection of fungal antagonists against Rhizoctonia solani by Grosch R, Faltin F, Lottmann J, Kofoet A, Berg G

“…Taxonomical characterization using molecular methods like BOX-PCR and sequencing of the 18S rDNA resulted in genetically diverse Trichoderma isolates. …”
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Vegetation–soil–microbiota dynamics across a 50-year reconstructed grassland chronosequence on the Loess Plateau of China by Yuanyuan Ma, Yan Shen, Ling Jin, Yu Tian, Hongbin Ma, Jian Lan, Bingzhe Fu

“…High-throughput sequencing was conducted to characterise rhizosphere microbial communities associated with alfalfa. …”
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Human endogenous retrovirus HERV-Fc1 association with multiple sclerosis susceptibility: a meta-analysis. by Belén de la Hera, Jezabel Varadé, Marta García-Montojo, Antonio Alcina, María Fedetz, Iraide Alloza, Ianire Astobiza, Laura Leyva, Oscar Fernández, Guillermo Izquierdo, Alfredo Antigüedad, Rafael Arroyo, Roberto Álvarez-Lafuente, Koen Vandenbroeck, Fuencisla Matesanz, Elena Urcelay

“…<h4>Background</h4>Human endogenous retroviruses (HERVs) are repetitive sequences derived from ancestral germ-line infections by exogenous retroviruses and different HERV families have been integrated in the genome. …”
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Prevalence and diversity of Helicobacter species in captive wild carnivores, and their implications for conservation management of endangered species by Wasim Uddin, Gulafsha Khan, Sneha Narayan, Neha Sharma, Basavaraj S. Holeyachi, Md. Abdul Hakeem, Archana Bharadwaj Siva, P. Anuradha Reddy

“…We investigated the prevalence and genetic diversity of Helicobacter species in thirteen wild carnivore species across twenty-two captive locations in India by targeting a partial region of the 16S rRNA gene. We used sequences obtained from positive samples for phylogenetic analysis, and evaluated factors influencing Helicobacter prevalence. …”
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Germline genetic variants were interactively associated with somatic alterations in gastric cancer by Xu Zhang, Yuzhuo Wang, Tian Tian, Gangqiao Zhou, Guangfu Jin

“…Abstract Genome‐wide association studies have identified several germline variants in gastric cancer. Meanwhile, sequencing studies have characterized extensive somatic alterations that arise during gastric carcinogenesis. …”
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A Single-Center Case Series of Hereditary Ataxia from Gujarat: Clinical and Genetic Data by Pranav Joshi, Shalin Shah, Mukesh Sumra, Sudhir Shah

“…Material and Methods: This prospective observational study included and evaluated chronic cerebellar ataxia patients with suspected hereditary etiology. …”
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Optimization of clustering parameters for single-cell RNA analysis using intrinsic goodness metrics by Nicolina Sciaraffa, Antonino Gagliano, Luigi Augugliaro, Claudia Coronnello, Claudia Coronnello

“…IntroductionThe accurate clustering of cell subpopulations is a crucial aspect of single-cell RNA sequencing. The ability to correctly subdivide cell subpopulations hinges on the efficacy of unsupervised clustering. …”
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Effect of Bifidobacterium animalis subsp. lactis Bi66 on Alleviating Constipation and Regulating Gut Microbiota by Zhixian CHEN, Ning PENG, Yan ZHANG

“…Objective: The objective of this study was to evaluate the efficacy of Bifidobacterium animalis subsp. lactis Bi66 in alleviating constipation in mice and exploring its mechanism, thereby providing a high-quality strain for native culture resources. …”
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Response of Microbial Community Structure and Changes in Carbon and Nitrogen Cycling Functional Genes during Ecological Restoration in Rocky Slopes Areas by Guo Mingfan, Tang Chuiyun, Zhang Jinxin, Lv Ying, Wei Li, Liu Xingyu

“…By spraying composite microbial agents (containing functional strains such as Clostridium and Desulfovibrio) and combining Illumina high-throughput sequencing with metagenomic analysis, we evaluated dynamic changes in soil fertility, microbial diversity, and functional genes over a 13 months restoration period. …”
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Matrix-free human lung organoids derived from induced pluripotent stem cells to model lung injury by Bettina Budeus, Chiara Kroepel, Lisa Marie Stasch, Diana Klein

“…We performed detailed morphological and molecular analyses, including bulk and single cell RNA sequencing, of generated lung organoids and evaluated the quality and robustness of our model as a potential in vitro platform for lung diseases, namely radiation-induced lung injury. …”
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HoloMoA: a holography and deep learning tool for the identification of antimicrobial mechanisms of action and the detection of novel MoA by Zohreh Sedaghat, Benoît Courbon, Héloïse Botrel, Hélène Dugua, Pawel Tulinski, Laethitia Alibaud, Lucia Pagani, Derry Mercer, Cyril Guyard, Christophe Védrine, Sophie Dixneuf

“…DIHM enables a label-free, time-resolved visualization of bacterial cell morphology and quantitative phase map to reveal phenotypic responses to antimicrobials, while DL techniques are powerful tools to extract discriminative features from image sequences and classify them. We assessed the performance of HoloMoA on Escherichia coli ATCC 25922 treated for up to 2 hours with 22 antibiotics representing 5 conventional functional classes (i.e. …”
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Geochemical characterisation and petroleum potential of selected cenozoic coals from Sarawak basin, Malaysia by Lanre Asiwaju, Khairul Azlan Mustapha, Wan Hasiah Abdullah, Yousif M. Makeen, Mohammed Hail Hakimi

“…The objectives were to evaluate thermal maturity, organic matter input, and hydrocarbon generation potential. …”
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Transcriptional landscape of pleural mesothelioma patients in relation to NF2 gene mutational status by Carlos Orozco-Castaño, Alejandro Mejía-Garcia, Hsuan Megan Tsao, Diego A. Bonilla, Carlos Carvajal-Fierro, Ricardo Bruges-Maya, Alba Combita, Rafael Parra-Medina

“…Logistic regression was used to evaluate associations with clinical variables, while transcriptomic differences were examined through differential expression and functional enrichment analyses. …”
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Construction of a myocardial and skeletal muscle-specific Cyr61 gene knockout mouse model by XU Diyan, ZHANG Wenli, ZUO Yidan, SU Zhen

“…The successful construction of conditional knockout mice was confirmed by identifying the Cyr61flox/flox, 3'LoxP and Cre enzyme sequences in mice. The knockout efficiency of Cyr61 was confirmed by Western blot. …”
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Hepatic transcriptome profiling unveils candidate genes in cattle with liver abscesses under the influence of beef genetics in dairy cattle. by Luana D Felizari, Sydney M Bowman, Chiquito J Crasto, Jhones O Sarturi, Dale R Woerner, Bradley J Johnson

“…This study aimed to evaluate the hepatic transcriptomic profile, immunohistochemistry, and IGF-I circulation in beef × dairy (Angus × Holstein) steers with and without liver abscesses. …”
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P36 | BIOLOGICAL ROLE OF DNA G-QUADRUPLEX DURING LIVER REGENERATION AFTER PARTIAL HEPATECTOMY

“… Guanine-rich DNA sequences can form four-stranded secondary structures known as G-quadruplexes (G4s). …”
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Mutational profiling using liquid biopsy to guide targeted therapy in patients with metastatic cancer by Omayma Mazouji, Abdelhak Ouhajjou, Naima Anouar, Chakib Nejjari, Roberto Incitti, Hicham Mansour

“…Genetic testing was conducted in patients with metastasis after failure of standard treatments. Sequencing was conducted in plasma-ctDNA samples; and when it was possible on the tumor tissue as well. …”
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