LAMA3 overexpression enhances proliferation, migration and invasion in esophageal squamous cell carcinoma based on bioinformatics and experimental validation by Xia Wu, Qian Li, Junbin Wang, Lei Li, Mingtang Zuo, Yufeng Cheng

“…In this study, we analyzed public RNA-sequencing data from TCGA and TIMER to evaluate LAMA3 expression and its clinical relevance in ESCC. …”
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Biotin-Thiamine-Responsive Basal Ganglia Disease: A Case Report by Jonathan Tse, Asem Abu-Qamar, Omar Youssef, Sherry L. Pejka

“…Extensive laboratory evaluations revealed lactic acidosis. MRI brain showed symmetric restricted diffusion affecting the bilateral basal ganglia, thalami, and cortical regions. …”
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Characterization of oral swab samples for diagnosis of pulmonary tuberculosis. by Rachel C Wood, Alfred Andama, Gleda Hermansky, Stephen Burkot, Lucy Asege, Mukwatamundu Job, David Katumba, Martha Nakaye, Sandra Z Mwebe, Jerry Mulondo, Christine M Bachman, Kevin P Nichols, Anne-Laure M Le Ny, Corrie Ortega, Rita N Olson, Kris M Weigel, Alaina M Olson, Damian Madan, David Bell, Adithya Cattamanchi, William Worodria, Fred C Semitala, Akos Somoskovi, Gerard A Cangelosi, Kyle J Minch

“…A qPCR targeting conserved bacterial ribosomal rRNA gene (rDNA) sequences was used to quantify bacterial biomass in samples. …”
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Bifidobacterium bifidum CCFM1389 Regulates Intestinal Motility Disorder by Repairing the Enteric Nervous System and Intestinal Barrier by HUANG Yin, LI Jiazhen, LIU Wenxu, XUE Yifan, ZHU Shengnan, WANG Linlin, WANG Gang

“…Bifidobacterium bifidum CCFM1167 was used as a positive strain, and another four B. bifidum strains were screened and evaluated for their regulatory effects on intestinal dysfunction and the ENS. 16S rDNA gene sequencing was used to detect changes in the gut microbiota, and hematoxylin-eosin (H&E) staining and real-time polymerase chain reaction (PCR) were employed to characterize the damage of the ENS and the intestinal barrier. …”
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Targeted Therapy for Metastatic Renal Cell Carcinoma by Andika Afriansyah, Agus Rizal AH. Hamid, Chaidir A Mochtar, Rainy Umbas

“…In the majority of trials evaluating targeted therapy, patients were stratified according to Memorial Sloan Kattering Cancer Center (MSKCC) risk model and the recommendation of targeted treatment based on risk features. …”
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A retrospective study on blood microbiota as a marker for cognitive decline: implications for detecting Alzheimer’s disease and amnestic mild cognitive impairment in Republic of Ko... by Youngchan Park, Jong-Young Lee, Eek-Sung Lee

“…Logistic regression models were used to evaluate the predictive impact of the microbiota on cognitive decline. …”
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Mutation status and prognostic value of KRAS and NRAS mutations in Moroccan colon cancer patients: A first report. by Fatima El Agy, Sanae El Bardai, Ihsane El Otmani, Zineb Benbrahim, Ibn Majdoub Hassani Karim, Khalid Mazaz, El Bachir Benjelloun, Abdelmalek Ousadden, Mohammed El Abkari, Sidi Adil Ibrahimi, Laila Chbani

“…RAS testing was investigated by Sanger sequencing and Pyrosequencing technology. BRAF (exon 15) status was analyzed by the Sanger method. …”
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Vaginal microbiota of American Indian women and associations with measures of psychosocial stress. by Joanna-Lynn C Borgogna, Michael Anastario, Paula Firemoon, Elizabeth Rink, Adriann Ricker, Jacques Ravel, Rebecca M Brotman, Carl J Yeoman

“…Self-collected mid-vaginal swabs were profiled for bacterial composition by 16S rRNA gene amplicon sequencing and metabolites quantified by targeted liquid-chromatography mass spectrometry. …”
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Yishen paidu pills attenuates 5/6 nephrectomy induced kidney disease via inhibiting the PI3K/AKT/mTOR signaling pathway by Saiji Liu, Yiling Cao, Qian Yuan, Yaru Xie, Yuting Zhu, Lijun Yao, Chun Zhang

“…Next, we conducted a 5/6 subtotal nephrectomy (5/6 SNx) rat model and treated these rats with YSPDP or Losartan for 10 weeks to evaluate the effect of YSPDP on CKD. To further analyze the underlying mechanism of YSPDP in CKD, the kidney tissues of 5/6 SNx rats treated with vehicle and YSPDP were performed with transcriptome sequencing. …”
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Population Genomics Reveals Small‐Scale Metapopulation Structure of Two Strictly Aquatic Keystone Species in a Recently Restored Urban River System (Emscher, Germany) by Martina Weiss, Marie V. Brasseur, Armin W. Lorenz, Florian Leese

“…Our study shows how genetic markers can support the assessment of population connectivity and thus evaluate the success of ecological restoration.…”
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Broadening the paradigm of laminin α2-related muscular dystrophy: A case of partial merosin deficiency with compound heterozygous variants by Azita Tavasoli, Shayan Eghdami, Maryam Kachuei, Saman Rouzbeh

“…We report a case of a 4-month-old girl presenting with delayed head control, axial hypotonia, and proximal muscle weakness, while cognitive and cardiac functions remained preserved. Laboratory evaluations revealed elevated serum creatine phosphokinase and lactate dehydrogenase levels. …”
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SHCBP1 drives tumor progression in triple-negative breast cancer by Huiling Wang, Huijuan Dai, Liheng Zhou, Yanping Lin, Wenjin Yin, Jingsong Lu

“…This study investigates the oncogenic functions of SHCBP1 in TNBC.Materials and methodsBulk RNA-seq and single-cell sequencing (scRNA-seq) data for TNBC samples were acquired from the Cancer Genome Atlas (TCGA) dataset and GSE161529, respectively. …”
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Rapid and Efficient DNA Extraction Protocol from Peruvian Native Cotton (<i>Gossypium barbadense</i> L.) Lambayeque, Peru by Luis Miguel Serquén Lopez, Herry Lloclla Gonzales, Wilmer Enrique Vidaurre Garcia, Ricardo Leonidas de Jesus Velez Chicoma, Mendoza Cornejo Greta

“…Furthermore, the extracted DNA was suitable for advanced applications, such as Sanger sequencing, by which high-quality electropherograms were obtained. …”
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Umbilical Cord-Derived Mesenchymal Stem Cells Attenuate S100-Induced Autoimmune Hepatitis via Modulating Th1 and Th17 Cell Responses in Mice by Xiaofeng Wei, Xinhong Cheng, Yang Luo, Xun Li

“…At the peak of the disease, serum levels of alanine aminotransferase and aspartate aminotransferase and pathologic damage to liver tissue were measured to evaluate liver function and degree of inflammation. …”
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Climate-smart crop production can support household food and nutrition security in urban communities in Chitungwiza, Zimbabwe by Blessing Nyamasoka-Magonziwa, Esther Simango, Hatirarami Nezomba

“…Adopting climate-smart agriculture (CSA) practices can contribute to mitigating this challenge.MethodsA study was carried out to evaluate current climate-smart crop production practices by urban farmers in Chitungwiza District, Zimbabwe, and their effects on household diets. …”
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Modulatory effects of Kratom extract on the gut microbiota of rats: implications for health by Nattaya Thongsepee, Sumet Amonyingcharoen, Pholasit Chamod, Wanwisa Himakhun, Kant Sangpairoj, Pongsakorn Martviset, Pathanin Chantree, Phornphan Sornchuer

“…Fresh fecal samples were collected and analyzed for changes in gut microbiome composition using 16S rRNA sequencing. Hematological parameters and lipid profiles were also measured to evaluate any systemic effects. …”
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C1q deficiency with severe skin manifestations: A case report from Pakistan by Kashmala Hussain, Shamila Ladak, Fizza Akbar, Salman Kirmani, Sadaf Altaf

“…Upon admission to our facility, clinical and laboratory evaluations indicated severe immunodeficiency. Whole exome sequencing (WES) identified a novel homozygous likely pathogenic variant in the C1qA (c..474C > G, p.Tyr158*), leading to C1q deficiency. …”
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Switched CMOS current source compared to enhanced Howland circuit for bio-impedance applications by da Silva Pablo Dutra, Filho Pedro Bertemes

“…EHCS is also used when the current driver is driven by a pseudo-random signal like discrete interval binary sequences (DIBS), which, due to its broadband nature, requires high performance operational amplifiers. …”
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Analysis of HPV and NKG2D rs1049174 Polymorphism in Vietnamese Patients With Oropharyngeal Squamous Cell Carcinoma by Nguyen Van Hung MD, Nguyen Hoang Viet PhD, Do Trung Duc MD, Tran The Dieu MD, Nguyen Thanh Binh MD, Le Huy Hoang PhD, Nguyen Quang Trung MD, PhD

“…HPV DNA was screened and typed using PCR and Sanger sequencing. Genotyping of NKG2D rs1049174 was performed using TaqMan assays. …”
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Genome wide association study of Fusarium wilt resistance in Spinacia turkestanica by Sanjaya Gyawali, Gehendra Bhattarai, James C. Correll, Ainong Shi, Lindsey J. du Toit

“…Single nucleotide polymorphism (SNP) markers (n = 7,065) identified with genotyping-by-sequencing (GBS) were used for genome wide association studies (GWAS) using multiple models tested with GAPIT and TASSEL software. …”
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