Metformin attenuates alveolar bone destruction in mice with apical periodontitis and inhibits pro-inflammatory cytokine synthesis in lipopolysaccharide-stimulated RAW264.7 through... by Chunmei Ren, Kento Tazawa, Nobuyuki Kawashima, Risa Ohshima, Yamato Okada, Shihan Wang, Ziniu Yu, Peifeng Han, Yujin Ohsugi, Yujin Ohsugi, Yujin Ohsugi, Sayaka Katagiri, Sayaka Katagiri, Takashi Okiji, Takashi Okiji

“…The aim of this study was to evaluate the effect of systemic metformin administration on experimentally induced apical periodontitis development in an animal model and clarify the underlying anti-inflammatory mechanism of metformin in lipopolysaccharide-stimulated mouse macrophages.MethodsEvaluations on the effects of metformin on the progression of periapical lesions were conducted in experimentally induced mouse apical periodontitis in vivo, and its anti-inflammatory effects in lipopolysaccharide-stimulated RAW264.7 macrophages in vitro were analyzed.ResultsMetformin significantly reduced periapical bone destruction on postoperative days 21 and 28, and decreased the number of osteoclasts on the periapical alveolar bone on postoperative day 28. …”
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Association of <i>SLCO1B3</i> and <i>SLCO1B1</i> Polymorphisms with Methotrexate Efficacy and Toxicity in Saudi Rheumatoid Arthritis Patients by Rania Magadmi, Ahlam M. Alharthi, Lina A. Alqurashi, Ibtisam M. Jali, Zeina W. Sharawi, Maha H. Jamal, Yasser Bawazir, Mohammad Mustafa, Sami M. Bahlas, Basma T. Jamal, Hassan Daghasi, Abdulrahman S. Altowairqi, Dalal Sameer Al Shaer

“…Genotyping of <i>SLCO1B3</i> (rs4149117, rs7311358) and <i>SLCO1B1</i> (rs2306283, rs4149056) polymorphisms was performed using Sanger sequencing. Statistical analyses, including logistic regression and haplotype analysis, were conducted to evaluate associations between these polymorphisms, MTX efficacy, and toxicity. …”
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Immunogenicity and protective efficacy of a multi-antigenic adenovirus-based vaccine candidate against Mycobacterium tuberculosis by Jin-Seung Yun, Jin-Seung Yun, Eunkyung Shin, Young-Ran Lee, Jung-Ah Lee, Hyeokjin Lee, Jong-Seok Kim, Sung Jae Shin, Sang-Jun Ha, Sang-Won Lee, Dokeun Kim, Jung-Sik Yoo, Hye-Sook Jeong

“…RNA sequencing of the whole blood of rAd-TB4-vaccinated mice collected pre- and, 1 and 4 weeks post-infection, identified differentially expressed genes associated with immune and inflammatory responses, especially those in the Wnt signaling pathway.ConclusionOur results indicate that rAd-TB4 immunization enhances the immune response to the vaccine boosting antigens in BCG-primed mice, making it a potential adult pulmonary TB vaccine candidate.…”
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A novel USP4 inhibitor that suppresses colorectal cancer stemness by promoting β-catenin and Twist1 degradation by Fengtian Li, Yi Zhou, Xinyue Lin, Yaxin Zhang, Qingyong Hu, Enen Zhao, Huali Li, Xingyan Pan, Feng Shu, Kun Zhang, Chengmei Huang, Na Tang, Wenting Liao

“…Gain- and loss-of-function experiments were performed to evaluate the function of USP4 in regulation of stemness and drug sensitivity in CRC. …”
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S-propargyl-cysteine attenuates temporomandibular joint osteoarthritis by regulating macrophage polarization via Inhibition of JAK/STAT signaling by Wenyi Cai, Antong Wu, Zhongxiao Lin, Wei Cao, Janak L. Pathak, Richard T. Jaspers, Rui Li, Xin Li, Kaihan Zheng, Yufu Lin, Na Zhou, Xin Zhang, Yizhun Zhu, Qingbin Zhang

“…Methods A MIA-induced TMJ-OA rat model and LPS-stimulated RAW264.7 macrophages were employed to evaluate the effects of SPRC in vivo and in vitro. …”
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Design of a Humanized Immunotoxin Based on Pertuzumab-Derived scFv and Shiga-Like Toxin 2 Subunit A Against Breast Cancer: An In Silico Study by Zeinab Ghesmati, Khadijeh Ahmadi, Navid Nezafat, Farzaneh Vahedi, Mortaza Taheri-Anganeh, Seyyed Hossein Khatami, Hasan Ghasemi, Rahmatollah Soltani, Vahid Zarezade, Ahmad Movahedpour, Ahmad Movahedpour

“…The tertiary structure of the immunotoxin was modeled, refined, and evaluated. Protein-protein docking was performed to assess immunotoxin-receptor binding, and molecular dynamics simulations were used to evaluate immunotoxin stability. …”
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Antitumor Activity of USP7 Inhibitor GNE-6776 in Non-Small Cell Lung Cancer Involves Regulation of Epithelial-Mesenchymal Transition, Cell Cycle, Wnt/β-Catenin, and PI3K/AKT/mTOR P... by Lipeng Wu, Long Lin, Meng Yu, Huajian Li, Yuanye Dang, Huosheng Liang, Guangyang Chen, Halimulati Muhetaer, Guodong Zheng, Jingjing Li, Xuejing Jia, Bo Wu, Chuwen Li

“…<b>Methods:</b> USP7 expression was analyzed in lung cancer tissue using data from public databases. RNA sequencing and functional enrichment analyses were conducted to explore differentially expressed genes (DEGs) and potentially related pathways. …”
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Establishment and characterization of liver‐specific Apoa4‐Cre and Cyp2c11‐Cre rat models in juvenile and adult stages by Wei Dong, Xiang Gao, Feifei Guan, Jirong Pan, Wei Chen, Li Zhang, Lianfeng Zhang

“…Hematoxylin–eosin stain was used to evaluate the liver histopathologic changes. The blood biochemical analysis of several liver enzymes and liver lipid profile was performed to evaluate the liver function of Cre rats. …”
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Over-expression analysis of TaDWF4˗4B in mutant wheat lines identifies a candidate regulator of heat stress tolerance by Liaqat Shah, Muhammad Saeed, Muhammad Ibrahim, Waqas Ahmad, Abdul Rahman Umar, Ayan Sohail, Hongqi Si

“…Here we analyzed a bulk of wheat exotic, elite, synthetic and local wheat lines to evaluate the impact of heat stress on the physiological and biochemical parameters of different genotypes. …”
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Salivary gland protective and antiinflammatory effects of genistein in Sjögren’s syndrome by inhibiting Xist/ACSL4-mediated ferroptosis following binding to estrogen receptor-alpha... by Tianjiao Mao, Wei Wei, Bo Chen, Yixin Chen, Shuqi Liang, Guiping Chen, Zhuoyuan Liu, Xiaodan Wu, Lihong Wu, Xiaomeng Li, Nobumoto Watanabe, Kevin H. Mayo, Janak L. Pathak, Jiang Li

“…Western blotting and immunohistochemistry analysis were used to evaluate the protein expression. RNA sequencing and RNA fluorescence in situ hybridization were employed to verify the relationship between Xist and ACSL4. …”
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Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective by Jennine Lection, Jennine Lection, Jennine Lection, Emily Van Syoc, Emily Van Syoc, Emily Van Syoc, Asha Miles, Asha Miles, Julia Hamilton, Marcela Martinez, Santiago Bas, Justin Silverman, Justin Silverman, Justin Silverman, Adrian Barragan, Erika Ganda, Erika Ganda, Erika Ganda

“…Vaginal discharge samples were analyzed with 16S rRNA sequencing to evaluate changes in the microbiome between treatments.ResultsAfter treatment, there were only minor differences within the microbiome between the two treatments indicating the potential suitability of dextrose as an antibiotic-alternative treatment. …”
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Chaotic Mountain Gazelle Optimizer Improved by Multiple Oppositional-Based Learning Variants for Theoretical Thermal Design Optimization of Heat Exchangers Using Nanofluids by Oguz Emrah Turgut, Mustafa Asker, Hayrullah Bilgeran Yesiloz, Hadi Genceli, Mohammad AL-Rawi

“…The efficiency of the proposed algorithm is evaluated using the challenging benchmark functions from various CEC competitions. …”
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Knockdown of human-specific lncRNA MEK6-AS1 improves osteogenic differentiation of senescent mesenchymal stem cells by CHEN Yunhua, LI Di, ZHAO Xiaoyan, CHEN Ming, LI Hongling, ZHAO Chunhua

“…The effect of MEK6-AS1 as an intervention target at osteogenic differentiation of human senescent hMSCs was evaluated with alkaline phosphatase staining microscopy. …”
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Combining Circular and Gauss-Markov Mobility Models for FANET Enhancement  by Suhad Faisal Behadili, Alyaa Safaa Abdulhameed

“…The first two scenarios implied the models independently, while the last two scenarios implied the proposed model (CCGM); each scenario was evaluated with Ad-hoc On Demand Distance Vector (AODV), Destination Sequenced Distance Vector (DSDV), and Greedy Perimeter Stateless Routing (GPSR) routing protocols. …”
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Identifying Predictors of Taxane-Induced Peripheral Neuropathy Using Mass Spectrometry-Based Proteomics Technology. by Emily I Chen, Katherine D Crew, Meghna Trivedi, Danielle Awad, Mathew Maurer, Kevin Kalinsky, Antonius Koller, Purvi Patel, Jenny Kim Kim, Dawn L Hershman

“…Recent advances in genome-wide genotyping and sequencing technologies have supported the discoveries of a number of pharmacogenetic markers that predict response to chemotherapy. …”
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Comparison of HIV-1 A6 dispersal dynamics in Poland before and after the war in Ukraine. by Karol Serwin, Kaja Mielczak, Anna Urbańska, Bogusz Aksak-Wąs, Malwina Karasińska-Cieślak, Piotr Ząbek, Ewa Siwak, Iwona Cielniak, Elżbieta Jabłonowska, Paweł Jakubowski, Błażej Rozpłochowski, Aleksandra Szymczak, Bartosz Szetela, Anna Kalinowska-Nowak, Monika Bociąga-Jasik, Elżbieta Mularska, Adam Witor, Anita Olczak, Władysław Łojewski, Maria Hlebowicz, Miłosz Parczewski

“…We created a dataset of 13,696 unique HIV-1 pol gene fragments of sub-subtype A6 including 1,889 sequences from Poland. To evaluate the import of distinct clusters and estimate dispersal dynamics, we performed time calibration of the maximum-likelihood phylogenetic trees and phylogeographic inferences using the software package BEAST with discrete and continuous diffusion models. …”
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MRI-based radiomic and machine learning for prediction of lymphovascular invasion status in breast cancer by Cici Zhang, Minzhi Zhong, Zhiping Liang, Jing Zhou, Kejian Wang, Jun Bu

“…The area under the receiver operating characteristic curve (AUC), accuracy, sensitivity, and specificity were used to evaluate the performance of the models. Results Eighteen radiomic features were retained to construct the radiomic signature. …”
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Potential antimicrobial and fruit juice clarification activity of amylase enzyme from Bacillus strains by Khondoker Moazzem Hossain, Umama Khan, S.M. Mahbubur Rahman, Md. Salauddin Khan

“…The research aimed to characterize amylase-producing bacteria and evaluate their potential for amylase activity regarding antimicrobial and fruit juice clarification. …”
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Reproducibility of magnetic resonance fingerprinting-based T1 mapping of the healthy prostate at 1.5 and 3.0 T: A proof-of-concept study. by Nikita Sushentsev, Joshua D Kaggie, Rhys A Slough, Bruno Carmo, Tristan Barrett

“…The aim of this proof-of-concept study was, therefore, to evaluate the cross-system reproducibility of prostate MRF T1 in healthy volunteers (HVs) using 1.5 and 3.0 T MRI systems. …”
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Identification of algD and oprL genes in Pseudomonas aeruginosa isolated from multiple environments and different local sources in Iraq

“…The aim of this study was to investigate the spread of the genes algD and oprL in P. aeruginosa within the genetic structure of environmental sources of isolates and various clinical studies, and to evaluate a successful alternative for diagnosing Pseudomonas aeruginosa (P. aeruginosa) among various sources. …”
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