Unveiling the mechanisms of CEBPD-orchestrated TAM polarization through RGS2/PAR2 pathway and its impact on ICB efficacy in clear cell renal cell carcinoma by Jun Gu, Yifan Liu, Wang Zhou, Shun Zhang, Ke-Qin Dong, Jia-Xin Chen, Wen-Yan Li, Zi-Chang Liu, Yi-Fan Tang, Xiu-Wu Pan, Xin-Gang Cui, Hong-feng Zheng, Mu-chen Li, Tian-yue Yang, Jian-gui Liu, Zi-xuan Gong

“…Immunofluorescence and in-vitro polarization assay were used to evaluate the effect of CEBP delta (CEBPD) on TAMs. …”
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Acute myeloid leukemia-derived bone marrow mesenchymal cells exhibit improved support for leukemic cell proliferation by Mariane Cristina do Nascimento, Diego A. Pereira-Martins, João Agostinho Machado-Neto, Eduardo M. Rego

“…To address these issues, we performed a differential gene expression analysis using RNA-sequencing data generated from healthy donors (HDs) and leukemic BMMSCs. …”
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Transcriptome analysis of the aortic coarctation area by Rada Ellegård, Torsten Malm, Constance G. Weismann, Eva Fernlund, Anneli Nordén Björnlert, Hanna Klang Årstrand, Katarina Ellnebo-Svedlund, Cecilia Gunnarsson

“…Methods: Tissue samples from 21 pediatric patients operated for CoA were dissected into separate biopsies consisting of the localized coarctation itself, proximal/distal tissue and ductus. RNA was sequenced to evaluate gene expression in the different biopsies. …”
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Advancing the understanding and management of angioimmunoblastic T-cell lymphoma: insights into its pathogenesis, clinical features, and emerging therapeutic strategies by Yurou Xing, Junmeng Huang, Yi Zhang, Yongsheng Wang, Shaochong Qi

“…Increasing clinical studies are evaluating new potential targets for AITL based on specific molecular markers, gradually improving individualized treatment and ultimately improving the clinical prognosis of patients with AITL. …”
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MEK inhibitors for the treatment of immunotherapy-resistant, AGK-BRAF fusion advanced acral melanoma: a case report and literature review by Yanling Zhang, Xifeng Zhang, Weikang Shao, Ji Gao, Mei Xiang, Yan Wang, Mengmeng Liu, Weizhen Zhang, Xianbin Liang

“…Methods We analyzed tumor tissue samples from the primary and metastatic lesions of the patient using next-generation sequencing (NGS) for genomic profiling and multiplex immunohistochemistry (mIHC) to assess the immune microenvironment. …”
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Deoxyribonucleic acid extraction for invertebrate metabarcoding from stream sediment and implication to biotic indices by M.H.F. Amin, H.W. Kim, I.A. Pratiwi, A.R. Kim, H.E. Kang, N.I. Oktavitri, T. Soedarti

“…Previous studies have focused on nucleic acid yield and taxonomic composition, but limited research has evaluated the impact of extraction methods on ecological assessments. …”
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PRECISION MEDICINE IN CLL: TREATMENT BASED ON MOLECULAR PROFILES by Esra Terzi Demirsoy

“…The most commonly used genetic tests are FISH (Fluorescence In Situ Hybridization) and next-generation sequencing (NGS). Conventional cytogenetics is not preferred in CLL due to its lower sensitivity for detecting smaller chromosomal abnormalities and subtle genetic mutations. …”
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Influence of weather and seasonal factors on whitefly dynamics, associated endosymbiotic microbiomes, and Begomovirus transmission causing tomato leaf curl disease: insights from a... by S. Sujatha, Kopparthi Amrutha Valli Sindhura, Prasanna S. Koti, Shridhar Hiremath, Mantesh Muttappagol, Mantesh Muttappagol, H. D. Vinay Kumar, K. S. Shankarappa, V. Venkataravanappa, K. M. Srinivas Reddy, C. N. Lakshminarayana Reddy

“…A more in-depth analysis of the microbial communities associated with B. tabaci, utilizing 16S rRNA metagenomic sequencing, revealed a dominance of the Proteobacteria phylum. …”
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Blocking the SIRPα-CD47 axis promotes macrophage phagocytosis of exosomes derived from visceral adipose tissue and improves inflammation and metabolism in mice by Yun-kai Lin, Yu-fei Pan, Tian-yi Jiang, Yi-bin Chen, Tai-yu Shang, Meng-you Xu, Hui-bo Feng, Yun-han Ma, Ye-xiong Tan, Hong-yang Wang, Li-wei Dong

“…We identified obesity-related miRNAs in AT-Exos and further explored their mechanisms using gain- and loss-of-function experiments. To evaluate the metabolic effects of AT-Exos on adipocytes, we conducted RNA-sequencing (RNA-seq) and confirmed our findings through Quantitative Real-time PCR (qPCR) and Western bolt analyses. …”
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Mesenchymal stromal cells conditioned by peripheral blood mononuclear cells exert enhanced immunomodulation capacities and alleviate a model of Myasthenia Gravis by Alexandra C. Bayer, Natalia Pinzón, Axel You, Cinthia Bergman, Nadine Dragin, Aurélien Corneau, Frédérique Truffault, Danièle Noël, Christophe Martinaud, Rozen Le Panse, Sonia Berrih-Aknin, Jean-Thomas Vilquin

“…Methods Leveraging a multi-omics approach encompassing RNA sequencing, flow and mass cytometry, secretome analysis, completed by functional evaluations, we investigated the mechanisms underpinning PBMC conditioning of MSC in vitro and their benefits in an animal model of Myasthenia gravis. …”
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Integrated single-cell and transcriptomic analysis of bone marrow-derived metastatic neuroblastoma reveals molecular mechanisms of metabolic reprogramming by Jing Chu, Rong Qin, Shu-Jing Wang, Qiang Wang, Qiang Wu

“…Furthermore, co-expression network analysis was used to evaluate the relationships between candidate genes and known NB regulatory factors. …”
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An efficient DNA Extraction Method from Ruditapes philippinarum larvae and Its Application in Genetic Analysis by Lei ZHANG, Xinghong XU, Zhihong LIU, Biao WU, Liqing ZHOU, Zhuanzhuan LI, Peizhen MA, Xiujun SUN

“…The larval DNA quality was evaluated using a NanoDrop UV-Vis Spectrophotometer, agarose gel electrophoresis, and DNA sequencing.The results demonstrated that PCR products amplified by 16SrRNA and COX1 showed a single clear band of target products, corroborated by their precise sequences. …”
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An exploratory study of INPP4B, a biomarker of gemcitabine chemoresistance in pancreatic cancer by REN Jiaqiang, WU Shuai, SU Tong, LI Jie, HAN Liang, WU Zheng

“…Clinical and prognostic information was collected, and the log-rank test was used to evaluate the relationship between target molecule expression and patient prognosis. …”
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Investigating the mechanisms by which low NAT1 expression in tumor cells contributes to chemo-resistance in colorectal cancer by Zheng Yuan, Kai Fang, Xinsheng Miao, Yan Zhang, Menghui Gu, Wei Xu, Hao Li, Dawei Zhu, Jiahui Zhou, Jian Sun, Xinhua Gu

“…Methods We conducted a comprehensive analysis using various bioinformatics tools and in vitro experiments to evaluate the effect of NAT1 expression on chemo-resistance in CRC. …”
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Interpretable prediction model for hand-foot-and-mouth disease incidence based on improved LSTM and XGBoost by Xiao LI, Shuyu HE, Yan PENG, Rongxin YANG, Lu TAO, Tingqi LOU, Wenqi HE

“…Through differencing, autoregression, and moving average operations, the ARIMA model effectively extracts structural features from time-series data and generates initial prediction results, along with residual sequences. These residual sequences contain complex information that the ARIMA model fails to fully capture, providing a foundation for the subsequent nonlinear analysis. …”
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Reproducible Effects of Sex and Acquisition Order on Multiple Global Signal Metrics: Implications for Functional Connectivity Studies of Phenotypic Individual Differences Using fMR... by Henry W. Chase, Danella M. Hafeman, Merage Ghane, Alexander Skeba, Tyler Brady, Haris A. Aslam, Richelle Stiffler, Lisa Bonar, Simona Graur, Genna Bebko, Michele Bertocci, Satish Iyengar, Mary L. Phillips

“…This may be due to low power, methodological differences across studies, including the use of global signal correction (GSR), or uncontrolled characteristics of the population. Methods Here, we evaluated measures of FC, regional variance, and global signal (GS) across six functional MRI (fMRI) sequences of different tasks and resting states and their relationship with individual differences in self‐reported measures of symptoms of depression, anxiety, impulsivity, reward sensitivity, and sensation seeking, as well as demographic variables and acquisition order, within groups of distressed and healthy young adults (18–25 years old). …”
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A global epidemic serotype 14 Streptococcus pneumoniae switching to non-vaccine types by Jinglin Yue, Lei Chen, Tingzhu Yao, Pengcheng Du, Chaoyang Gu, Hengkun Wei, Kai Han, Chengbo Rong, Chenchen Wang, Qin Zhang, Chen Chen, Jingyuan Liu, Mingxi Hua

“…More importantly, as a strain that is already prevalent, the novel NVT strain possesses the potential to become a global pandemic strain.IMPORTANCEThe study employed high-throughput sequencing to analyze Streptococcus pneumoniae isolates from Xiamen Children’s Hospital, China, to evaluate vaccine effectiveness in light of serotype changes and documented the occurrence of a serotype switch (from 14 to 15B) between vaccine-type and non-vaccine-type serotypes in the ST4749 strain. …”
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B-Cell Epitope Mapping of the <i>Treponema pallidum</i> Tp0435 Immunodominant Lipoprotein for Peptide-Based Syphilis Diagnostics by Jessica L. Keane, Mahashweta Bose, Barbara J. Molini, Kelika A. Konda, Silver K. Vargas, Michael Reyes Diaz, Carlos F. Caceres, Jeffrey D. Klausner, Rebecca S. Treger, Lorenzo Giacani

“…Recognized amino acid sequences were then mapped to the experimentally determined Tp0435 structure. …”
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Deep learning‐based analysis of EGFR mutation prevalence in lung adenocarcinoma H&E whole slide images by Jun Hyeong Park, June Hyuck Lim, Seonhwa Kim, Chul‐Ho Kim, Jeong‐Seok Choi, Jun Hyeok Lim, Lucia Kim, Jae Won Chang, Dongil Park, Myung‐won Lee, Sup Kim, Il‐Seok Park, Seung Hoon Han, Eun Shin, Jin Roh, Jaesung Heo

“…For the test dataset, 197 WSIs were collected from Ajou University Medical Center to evaluate the presence of EGFR mutations. Our model demonstrated prediction performance with an area under the receiver operating characteristic curve of 0.7680 (0.7607–0.7720) and an area under the precision‐recall curve of 0.8391 (0.8326–0.8430). …”
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Gut microbiome is associated with radiotherapy response in lung cancer patients with brain metastases by Fei Liang, Yichu Sun, Jing Yang, Ziqiang Shen, Guangfeng Wang, Jiangrui Zhu, Chong Zhou, Youyou Xia, Youyou Xia

“…Fecal samples were collected before radiotherapy and analyzed by 16S rRNA sequencing to assess the gut microbiome’s composition and function. …”
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