Unraveling the relationships between midge abundance and incidence, microbial communities, and soil and water properties in a protected natural tallgrass prairie by Saraswoti Neupane, Travis Davis, Cassandra Olds, Dana Nayduch, Bethany L. McGregor

“…Midge incidence and abundance were evaluated using emergence assays, which assessed the number of adults emerging from collected soil samples, and bacterial and protistan communities in these samples were characterized through amplicon sequencing of the 16S and 18S rRNA genes. …”
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Gut dysbiosis narrative in psoriasis: matched-pair approach identifies only subtle shifts correlated with elevated fecal calprotectin by Bayazit Yunusbayev, Anna Bogdanova, Nadezhda Nadyrchenko, Lavrentii Danilov, Viktor Bogdanov, Grigory Sergeev, Radick Altinbaev, Fanil Bilalov, Milyausha Yunusbaeva

“…Hence, the dominant narrative about gut microbiome alterations’ impact on disease should be evaluated critically with more data and a well-powered approach. …”
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Syk inhibitor attenuates lupus in FcγRIIb− /− mice through the Inhibition of DNA extracellular traps from macrophages and neutrophils via p38MAPK-dependent pathway by Kritsanawan Sae-khow, Awirut Charoensappakit, Kanyarat Udompornpitak, Wilasinee Saisorn, Jiraphorn Issara-Amphorn, Tanapat Palaga, Asada Leelahavanichkul

“…Abstract Spleen tyrosine kinase (Syk), an important hub of immune signaling, is activated by several signalings in active lupus which could be interfered by Syk inhibitor but is still not completely evaluated in innate immune cells associated with lupus activity. …”
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Microbial community shifts during salt mitigation treatments of historic buildings using mineral poultices: a long-term monitoring of salt and associated biofilms by Johannes Tichy, Beate Sipek, Martin Ortbauer, Lukas Fürnwein, Monika Waldherr, Alexandra Graf, Katja Sterflinger, Guadalupe Piñar

“…Second, the effect of the desalination treatments on salt-associated pink biofilms was also evaluated. The shifts within the biofilm communities during and after the treatment were monitored by qPCR and long-read archaeal- and bacterial-16S rRNA amplicon analysis using the Nanopore sequencing technology. …”
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Mutations in TET2 and DNMT3A genes are associated with changes in global and gene-specific methylation in acute myeloid leukemia by Alberto Ponciano-Gómez, Adolfo Martínez-Tovar, Jorge Vela-Ojeda, Irma Olarte-Carrillo, Federico Centeno-Cruz, Efraín Garrido

“…The R882A hotspot of DNMT3A and exons 6–10 of TET2 were amplified by polymerase chain reaction and sequenced using the Sanger method. Methylation patterns of 16 gene promoters were evaluated by pyrosequencing after treating DNA with sodium bisulfite, and their transcriptional products were measured by real-time quantitative polymerase chain reaction.Here, we demonstrate altered levels of 5-methylcytosine and 5-hydroxymethylcytosine and highly variable transcript levels of DNMT3A and TET2 in peripheral blood leukocytes from acute myeloid leukemia patients. …”
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The Influence of TLR4, CD14, OPG, and RANKL Polymorphisms in Periodontitis: A Case-Control Study by Joana Maira Valentini Zacarias, Josiane Bazzo de Alencar, Patrícia Yumeko Tsuneto, Victor Hugo de Souza, Cléverson O. Silva, Jeane Eliete Laguila Visentainer, Ana Maria Sell

“…The risk (OR) was evaluated for values of P<0.05. Differences in TLR4, CD14, RANKL, and OPG genotype and allele frequency distributions were not observed between patients and controls. …”
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Lactate-Induced Mitochondrial Calcium Uptake 3 Aggravates Myocardial Ischemia–Reperfusion Injury by Promoting Neutrophil Extracellular Trap Formation by Hongru Zhang, Lei Liu, Chuchu Shen, Xinxue Jiang, Jing Liu, Jing Chen, Senlei Xu, Yanfei Mo

“…Immunofluorescence was employed to evaluate NET activation in tissues, peripheral blood neutrophils, and protein colocalization. …”
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Biological characteristics of Multidrug-Resistant Proteus mirabilis Bacteriophage and Its Antibacterial Effect on Chicken Breast Meat by PAN Shunyuan, CHEN Qibai, WANG Hongyue, KONG Xiangyu, PANG Shenyu, GAO Dongyang, SONG Jun

“…Subsequently, its genome was extracted and analyzed through whole genome sequencing. Crystal violet staining and plate counting were employed to assess the ability of the phage to remove biofilms and the efficiency in removing biofilms on different materials was evaluated using the plate counting method. …”
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Identification of the metabolic protein ATP5MF as a potential therapeutic target of TNBC by Kaiyan Chen, Yingchun Wu, Linfeng Xu, Changyong Wang, Jinqiu Xue

“…The animal model was usded to evaluated in vivo tumor response aftering ATP5MF silencing. …”
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Bacterial diversity, biogenic amines and lipids oxidation in traditional dried anchovy (Encrasicholina punctifer) during ambient storage by Ismail M. Al Bulushi, Nejib Guizani, Mutamed Ayyash, Mohammed Al Za'abi, Aisha Abushelaibi, Hilton C. Deeth, Zahra Al Kharousi, Fathiya Al Hamadani, Salha Al Maskari, Jamila Alkalbani

“…This study aimed to elucidate the effect of ambient storage (23&plusmn;2&deg;C, 68% RH) on the bacterial load and diversity, biogenic amines and lipids oxidation in traditional dried anchovy (E. punctifer) in order to evaluate its safety, quality and stability during 12 weeks of&nbsp;storage. …”
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Human endogenous retrovirus-K envelope protein is aberrantly expressed in serous ovarian cancer and promotes chemosensitivity via NF-κB/P-glycoprotein pathway inhibition by Que Thanh Thanh Nguyen, Soo-Gui Shin, Tra Tran Thu Nguyen, Kyoung Yul Lee, Michael McClelland, Eun-Ju Lee

“…Transcriptomes were evaluated using strand-specific reverse transcription-PCR and sequencing. …”
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Tanshinone I Ameliorates Psoriasis-Like Dermatitis by Suppressing Inflammation and Regulating Keratinocyte Differentiation by Wang Q, Luo X, Su Y, Jin Y, Kuang Q, Li S, Shen W, Zhu Y

“…Disease severity was evaluated using the Psoriasis Area and Severity Index (PASI), and histological changes were assessed via H&E staining and Ki67 immunofluorescence. …”
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Immunogenicity and Protective Efficacy of an mRNA Vaccine Targeting HSV-2 UL41 in Mice by Tangwei Mou, Yu Zhao, Jie Jia, Kai-Cheng Gao, Shao-You Li, Yi-Qun Kuang

“…Methods: The immunogenicity and protective effects of an HSV-2 UL41 mRNA vaccine were evaluated in a BALB/c mouse model. The mice were intramuscularly immunized twice, followed by HSV-2 infection at 28 days post boost. …”
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Assessing the suitability of formalin-fixed paraffin-embedded (FFPE) tissue for genome-wide association studies (GWAS) by Julian Reingruber, Maximilian J. Krämer, Jessica Bigge, Pouria Dasmeh, Sandeep Grover, Stefanie Heilmann-Heimbach, Markus M. Nöthen, Nicole Kreuser, Ines Gockel, Lothar Veits, Michael Vieth, Paul Jank, Carsten Denkert, Carlo Maj, Timo Hess, Johannes Schumacher

“…To explore the suitability of utilizing FFPE tissue for GWAS, we analysed the genotyping concordance between corresponding FFPE and blood samples. We evaluated both microarray technology and low-coverage whole-genome sequencing (lcWGS) to determine whether there were differences between genotyping methods. …”
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The emerging role of circulating tumor DNA in brain tumor research by Amir Modarresi Chahardehi, Niki Faraji, Nikoo Emtiazi, Reza Nasiri, Maryam Daghagheleh, Helia Mohammadaein, Fatemeh Masoudi, Kimia Ghazi Vakili, Aylin Sefidmouy Azar, Hossein Fatemian, Hossein Motedayyen, Reza Arefnezhad, Fatemeh Rezaei-Tazangi, Zahra Niknam, Marziye Ranjbar Tavakoli

“…Hence, advancements in next-generation sequencing (NGS) and digital PCR have enhanced the sensitivity of ctDNA detection, rendering it a feasible method for monitoring tumor dynamics and evaluating therapy responses. …”
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Assembly Task Allocation for Human&#x2013;Robot Collaboration Considering Stability and Assembly Complexity by Zhenting Wang, Takuya Kiyokawa, Natsuki Yamanobe, Weiwei Wan, Kensuke Harada

“…Four chairs from different manufacturers are used to evaluate the effectiveness of the proposed allocation method. …”
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TTLL4 mediates the PI3K/AKT/MDM2 pathway to promote hepatocellular carcinoma progression and predict patient prognosis by Zeping He, Desheng Chen, Lei Li, Shanbao Li, Fangbin Song, Jinfeng Cai, Xueyan Guo, Yaohao Luo, Xinshuai Wang, Zeping Chen, Junming Xu

“…In vivo, tumor growth and metastasis were evaluated through subcutaneous implantation and tail vein injection. …”
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Microencapsulated Microbial Seed Coating Could Improve Soil Environment and Maize Grain Yield in Saline Soil by Qiuyan Huo, Min Gong, Yawen Jiang, Xi Yang, Meng Kong, Jiuxing He, Qiang Zhang, Jiqing Song, Xinzhu Li, Wei Han, Xurong Mei, Guohua Lv

“…Field experiments were conducted to evaluate the effect of seed coating on soil environment and maize growth in saline soils. …”
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CD24 recruits tumor-associated neutrophils to promote the progression of hepatocellular carcinoma by Jun Wang, Jing Xiong, Yu Cheng, Yuan Wu, Shu Jiang, Huimin Zhang, Xiang Xiao, Hanning Li, Yimeng Liu, Jiapeng Li, Jincheng Wang, Zetao Song, Chaojiang Gu, Shaoyong Chen, Yuan Xiang, Xinghua Liao

“…Additionally, cell counting kit 8 and colony formation assays were conducted to evaluate the effect of CD24 on the sensitivity of HCC cells to sorafenib. …”
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Modulation of NLRP3 Inflammasome Activation by QYHT Decoction: Implications for the Treatment of Erectile Dysfunction in Hyperuricemia by Pingyu Ge, Yinxue Guo, Bangwei Che, Hang Jin, Lan Chen, Zhichao Chen, Kaifa Tang

“…This study employed four treatment methods: (a) treating HUA-induced ED patients with QYHT and analyzing changes in gut microbiota abundance and fecal metabolites through 16S sequencing; (b) establishing an HUA-induced ED rat model, treating with different doses of QYHT, and examining changes in serum metabolites; (c) conducting fecal microbiota transplantation (FMT) therapy; evaluating erectile function, oxidative stress, inflammatory response, and NLRP3 inflammasome activation levels; and (d) exploring key monomeric compounds and potential targets in QYHT through network pharmacology and molecular docking. …”
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