Arctiin Protects Chondrocytes from Interleukin-1β-Induced Inflammation and Apoptosis by RNA Sequence In vivo and In vitro by Yong-Jia Song, Jia-Min Bao, Zeng-Qiao Zhang, Yun-Xiang Hai, Hao-Nan Wen, Tian-Jun Zhai, Wei Feng, Min Song

“…Validation of gene and protein expression was carried out using qRT-PCR, Western blotting, and cellular flow cytometry, based on the results from sequencing. Furthermore, Safranin-O fast green staining and immunohistochemistry staining were performed on slices of the mice knee joint to evaluate the OA Research Society International score, alterations in the cartilage matrix, and levels of apoptosis-related proteins at sites of knee cartilage damage in an arthritis model induced by monosodium iodoacetate (MIA) and physical activity. …”
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Insights from expert elicitation: prioritising pathogens for genome sequencing for Australian public health responseResearch in context by Tehzeeb Zulfiqar, Angeline Ferdinand, Danielle Cribb, Patiyan Andersson, Alireza Zahedi, Kathryn Glass, Son Nghiem, Susan Trevenar, Nhung Mai, Benjamin P. Howden, Martyn D. Kirk

“…Summary: Background: Pathogen genomics has transformed infectious disease response, yet prioritisation frameworks for genome sequencing remain underdeveloped. This study establishes evidence-based criteria for prioritising pathogens for genome sequencing to maximise public health impact in Australia. …”
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Incremental yield of prenatal exome sequencing in fetuses with skeletal system abnormalities: A systematic review and meta‐analysis by Yan Wang, Yuan Lv, Jia Yao, Hao Ding, Gang Li, Jianmin Li, Lizhu Chen

“…Abstract Introduction Fetal skeletal abnormalities can be caused by various factors and genetic cause plays an important role. Prenatal exome sequencing (ES) has been shown to be a powerful approach for accurate prenatal molecular diagnoses. …”
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Transcriptome sequencing–based analysis of the molecular mechanism underlying the effect of lncRNA AC003090.1 on osteoporosis by Huafeng Zhuang, Yongjun Lin, Chengye Lin, Miao Zheng, Yizhong Li, Xuedong Yao, Youjia Xu

“…The expression levels of GSK-3β, β-catenin, and c-Myc were evaluated by performing RT-qPCR and Western blot analysis. …”
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Impact of a structured food sequence and mobile health monitoring on gestational diabetes outcomes: a clinical trial by Ria Murugesan, Shubhashree Thiruselvam, Kakithakara Vajravelu Leela, Abhishek Satheesan, K. Geetha, Mohan Ram, Janardanan Kumar

“…This study evaluates whether combining a structured food sequence with mHealth monitoring enhances outcomes in women with GDM.MethodsFifty-four pregnant women diagnosed with GDM were randomized into an intervention group (n = 27) and a control group (n = 27). …”
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Whole-transcriptome sequencing reveals the effects of acupuncture on early embryos post-IVF-ET in poor ovarian response by Jianheng Hao, Yuemeng Zhao, Xuan Liu, Huichao Xu, Liying Liu, Haijun Wang, Ying Lan, Laixi Ji

“…Histopathological changes and apoptosis in the ovaries were evaluated using HE and TUNEL staining. Whole-transcriptome sequencing was employed to establish expression profiles of differentially expressed mRNAs (DEmRNAs), DEmiRNAs, DElncRNAs, and DEcircRNAs. …”
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Whole-genome sequencing and metagenomics reveal diversity and prevalence of Listeria spp. from soil in the Nantahala National Forest by Jia Wang, Claire N. Schamp, Lauren K. Hudson, Harleen K. Chaggar, Daniel W. Bryan, Katie N. Garman, Mark Radosevich, Thomas G. Denes

“…Recently, metagenomic sequencing has also been explored as a tool for the detection of Listeria spp. in environmental samples. …”
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Comparison of Raman spectroscopy with mass spectrometry for sequence typing of Acinetobacter baumannii strains: a single-center study by Suling Liu, Ni Zhang, Jiawei Tang, Chong Chen, Weisha Wang, Jingfang Zhou, Long Ye, Xiaoli Chen, ZhengKang Li, Liang Wang

“…Further studies are warranted to evaluate its potential in nosocomial surveillance systems, especially for rapidly identifying outbreaks within hospitals.IMPORTANCEThe rapid and accurate sequence typing (ST) of bacterial pathogens is pivotal in controlling transmission within healthcare settings. …”
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In Vitro Study Comparing SARS-CoV-2 Ancestral and Omicron Lineages Sequence & Neutralization Differences by Ms Ashley Burroughs, Dr Clinton Paden, Neha Mittal, Juan Castro, Peter Cook, Sandra Lester, Heather Hicks, Dr. Azaibi Tamin, Fatimah Dawood, Jennifer DeCuir, Diya Surie, Dr. Melissa M Coughlin, Dr. Jennifer L. Harcourt, Dr. Natalie J. Thornburg, Dr. Lydia J. Atherton

“…This study compared sequence changes in the spike protein of SARS-CoV-2 between ancestral WA1/2020 and Omicron lineages and evaluated the impact of those sequence differences on neutralization. …”
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Nanopore full length 16S rRNA gene sequencing increases species resolution in bacterial biomarker discovery by Pablo Aja-Macaya, Kelly Conde-Pérez, Noelia Trigo-Tasende, Elena Buetas, Mohammed Nasser-Ali, Paula Nión, Soraya Rumbo-Feal, Susana Ladra, Germán Bou, Álex Mira, Juan A. Vallejo, Margarita Poza

“…However, recent developments in third-generation sequencing, such as Oxford Nanopore Technologies (ONT)’s new R10.4.1 chemistry and its improved basecalling models, are beginning to allow for a more complete and accessible species-level analysis through full-length 16S rRNA gene sequencing (spanning regions V1-V9). …”
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Rapid Whole-Genome Sequencing in Critically Ill Infants and Children with Suspected, Undiagnosed Genetic Diseases: Evolution to a First-Tier Clinical Laboratory Test in the Era of... by Rina Kansal

“…Factors that will help to develop rWGS as a clinical test in critically ill infants and the limitations are briefly discussed, including an evaluation of the clinical utility and accessibility of genetic testing, education for parents and providers, cost-effectiveness, ethical challenges, consent issues, secondary findings, data privacy concerns, false-positive and false-negative results, challenges in variant interpretation, costs and reimbursement, the limited availability of genetic counselors, and the development of evidence-based guidelines, which would all need to be addressed to facilitate the implementation of pediatric genomic sequencing in an effective widespread manner in the era of precision medicine.…”
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Former à évaluer des compétences en enseignement supérieur : un dispositif d’évaluation novateur by Nathalie Michaud, Martin Roy

“…The origin of the project comes from a desire to bond in a more rigorous and coherent way the teaching – assessment sequence within the course. This article aims to document the development of the innovative evaluation system put in place. …”
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Eruption of Permanent Teeth in Bulgarian Children Aged 5–12 Years by Boyan Valentinov Kirilov, Ivaila Yankova Ivanova-Pandourska, Yanitsa Velichkova Zhecheva

“…Differences between males and females are found in the sequence of eruption only of canines and premolars. …”
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Vascular-type heterogeneity is associated with differential gene expression profiles of endothelial cells under shear stress by Allaura A. Cox, Christopher James Ng

“…After shear stress exposure, cellular alignment was analyzed, and RNA was extracted and evaluated via bulk RNA sequencing. Results: All ECs demonstrated significant changes in alignment under shear stress. …”
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Molecular investigation of feline herpesvirus 1 (fhv-1) and feline calicivirus in cats with respiratory system problem by Seval Bilge Dağalp, Fırat Doğan, Touraj Aligholipour Farzani, Ali Rıza Babaoğlu, Gülizar Acar Kırmızı, Mehmet Çabalar

“…Samples with positive results were subjected to sequence analysis for molecular characterization.<p> <b>Results:</b> Sampled cats were evaluated as positive for 45.71% (32/70) FHV-1 and 10% (7/70) FCV. 4,29% (3/70) of these cats were positive for both infections. …”
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Electro-sequence valorization of specific enablers of aquifer vulnerability and contamination: A case of index-based model approach for ascertaining the threats to quality groundwa... by N.J. George, A.M. Ekanem, J.E. Thomas, N.I. Udosen, N.M. Ossai, J.G. Atat

“…The study used geo-electrical technology to evaluate the lithological sequence of the hydrogeological units and their first-and second-order geo-electric properties, as well as the geochemical constituents of groundwater. …”
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Clinical and genomic characterization of Klebsiella pneumoniae infections in Dhaka, Bangladesh by Zannat Kawser, Sushmita Sridhar, Sanchita Kar, Tanbir Habib, Sharmin Akter Mukta, Kasrina Azad, Neyamul Hasan, Umme Kulsum, Abu Bakar Siddik, Saikt Rahman, Nusrat Noor Tanni, Maherun Nesa, Ashlee M. Earl, Colin J. Worby, Sarah E. Turbett, SM Shamsuzzaman, Jason B Harris, Firdausi Qadri, Regina C LaRocque

“…We used standard microbiologic techniques to evaluate AMR and whole-genome sequencing (WGS) to assess dominant lineages, common capsular (K) and O-polysaccharide (O) antigen types, and AMR and virulence genes. …”
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Optimized multiplex PCR-NGS for comprehensive HLA genotyping in Chinese populations: resolving ambiguities at high resolution by Cuello Garcia Haider, Cuello Garcia Haider, Binbin Sun, Yinfeng Wang, Zhoufan Zhang, Changling Cao, Yiying Zhu, Ouzaouit Abdelhak, Huiqiang Huang, Haitao Liu, Tingya Jiang, Xueping Dong, Yang Zhou, Yu Wu

“…PThe primers were optimized using as reference the sequencing depth across loci. The method was validated using SBT and probe capture‑based targeted next‑generation sequencing to evaluate its approach accuracy. …”
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Gene Fusion Detection in Long-Read Transcriptome Datasets from Multiple Cancer Cell Lines by Keigo Masuda, Yoshiaki Sota, Hideo Matsuda

“…Background: Fusion genes are important biomarkers in cancer research because their expression can produce abnormal proteins with oncogenic properties. Long-read RNA sequencing (long-read RNA-seq), which can sequence full-length mRNA transcripts, facilitates the detection of such fusion genes. …”
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TRsv: simultaneous detection of tandem repeat variations, structural variations, and short indels using long read sequencing data by Shunichi Kosugi, Chikashi Terao

“…We demonstrated genome-wide detection of TR-CNVs, including variants associated with gene expression, disease, and quantitative traits, using 160 long-read whole genome sequencing data and TRsv.…”
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