Aspartame increases the risk of liver cancer through CASP1 protein: A comprehensive network analysis insights by Ni-ren Li, Yi-xuan Zeng, Yu-feng Gu, Pai Xie, Bing-ying Deng, Si-fan Lu, Wang-an Li, Yi Liu

“…Additionally, the single-cell sequencing found that CASP1 is most highly expressed in endothelial cells. …”
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Bridging aging, immunity, and atherosclerosis: novel insights into senescence-related genes by Yan Lu, Rong Yuan, Rong Yuan, Qiqi Xin, Qiqi Xin, Keji Chen, Keji Chen, Weihong Cong, Weihong Cong

“…Identifying reliable biomarkers and understanding the interactions between immune senescence and AS may provide important therapeutic opportunities for AS.MethodsThe RNA sequencing and the single-cell RNA sequencing (scRNA-seq) dataset were downloaded from the Gene Expression Omnibus datasets, with all data derived from human tissues. …”
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ViLR: a novel virtual long read method for breakpoint identification and direct SNP haplotyping in de novo PGT-SR carriers without a proband by Jiangyang Xue, Min Xie, Jie Cai, Kai Kang, Mengnan Gu, Mai Li, Haiyue Shi, Xin Zhang, Lingyin Kong, Bo Liang, Liming Zhou, Changshui Chen, Haibo Li

“…Abstract Background Despite the gradual application of third-generation long read sequencing (LRS) or reference embryo establishment to preimplantation genetic testing for structural rearrangement (PGT-SR) without familial involvement, there are still limitations to their extensive clinical application yet. …”
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Induction of apoptosis and hypoxic stress in malignant melanoma cells via graphene-mediated far-infrared radiation by Wumei Zhao, Ziwen Chen, Wenxing Fu, Chenyan Ye, Haijing Fu, Tianyi Xu, Binghui Wu, Lina Chen, Shi-Jun Shan

“…Methods This study systematically evaluated the inhibitory effects of FIR on MM through in vitro cell experiments, animal models, and molecular mechanism analysis. …”
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FGFR3-TACC3 fusion gene promotes glioblastoma malignant progression through the activation of STAT3 signaling pathway by Yiming Li, Yiming Li, Jianshen Liang, Jianshen Liang, Jianshen Liang, Xiude Ren, Xiude Ren, Jiahe Guo, Jiahe Guo, Jiahe Guo, Xisen Wang, Xisen Wang, Xisen Wang, Xuya Wang, Xuya Wang, Xuya Wang, Shengping Yu, Shengping Yu, Tao Li, Tao Li, Xuejun Yang, Xuejun Yang, Xuejun Yang

“…The underling mechanism were further inferred and validated through RNA sequencing, E-MTAB-6037 gene chip data, and western blot. …”
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Differential methylation of circulating free DNA assessed through cfMeDiP as a new tool for breast cancer diagnosis and detection of BRCA1/2 mutation by Piera Grisolia, Rossella Tufano, Clara Iannarone, Antonio De Falco, Francesca Carlino, Cinzia Graziano, Raffaele Addeo, Marianna Scrima, Francesco Caraglia, Anna Ceccarelli, Pier Vitale Nuzzo, Alessia Maria Cossu, Stefano Forte, Raffaella Giuffrida, Michele Orditura, Michele Caraglia, Michele Ceccarelli

“…Shallow whole genome sequencing was performed on the immuno-precipitated samples. …”
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Gr-1 blockade remodels the immunosuppressive microenvironment induced by incomplete microwave ablation of hepatocellular carcinoma by Tian Huang, Hensong Cao, Shipeng Dai, Yonghua Zhu, Hanyuan Liu, Shuxian Zhu, Zhengqing Lu, Chuan Liu, Chengyu Lv, Zhouxiao Li, Jinhua Song, Han Zhuo

“…The effects of iMWA on the genes of HCC cancer cells and the TIME were investigated by RNA sequencing, mass cytometry, immunohistochemistry, and immunofluorescence. …”
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Antiphospholipid antibodies inhibit the migration and invasion of trophoblast cells by suppressing the JNK/C-Jun/MMP1 signaling pathway by Qingchen Wang, Weimin Feng, Yuan Tan, Jiao Qiao, Hongchao Liu, Qi Liu, He Wang, Qian Zhang, Jingjin Tao, Zhongxin Li, Boxin Yang, Zhen Xu, Chong Wang, Shuo Yang, Liyan Cui

“…Method An APS cell model was established in the HTR-8 trophoblast cell line, followed by RNA sequencing to identify key genes involved in trophoblast cell function. …”
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Polystyrene nanoplastics trigger pyroptosis in dopaminergic neurons through TSC2/TFEB-mediated disruption of autophagosome-lysosome fusion in Parkinson’s disease by Xiaomei Liang, Yaqi Zeng, Piao Zhang, Baoyu Zhu, Jiezhu Feng, Tongtong Deng, Zhongling Fu, Chengshuai Liu, Chengyu Chen, Yuhu Zhang

“…Additionally, proteomic sequencing was utilized to identify the upstream regulator of the autophagy-lysosomal pathway (ALP), and the effects of modulating this target protein on the ALP-pyroptosis pathway were analyzed. …”
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Circulating microbiome DNA features and its effect on predicting clinicopathological characteristics of patients with colorectal cancer by Liang Ma, Mingliang Shi, Xue Zhang, Yan Liu, Hui Jin, Dan Li, Hui Zhang, Li Feng, Jing Zuo, Yudong Wang, Jiayin Liu, Jing Han

“…This study investigates the peripheral venous blood microbiome in CRC patients using 2bRAD-M sequencing and evaluates its clinical significance. …”
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Dexamethasone: a double-edged sword in the treatment of osteoarthritis by Karyna Tarasova, Maria Belen Arteaga, Angkana Kidtiwong, Sinan Gueltekin, Andrea Bileck, Christopher Gerner, Iris Gerner, Florien Jenner

“…This study evaluated the effects of DEX and TA on the expression of pro-inflammatory genes in inflamed chondrocytes. …”
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Structural and Functional Profiling of Water-Extracted Polypeptides from <i>Periplaneta americana</i>: A Multifunctional Cosmetic Bioactive Agent with Antioxidative and Anti-Inflam... by Xinyu Sun, Zhengyang Zhang, Jingyao Qu, Deyun Yao, Zeyuan Sun, Jingyi Zhou, Jiayuan Xie, Mingyang Zhou, Xiaodeng Yang, Ling Wang

“…HPLC-MS analysis identified 1402 peptide sequences, 80.51% of which were below 1000 Da, predominantly consisting of tri-, tetra-, and octapeptides. …”
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The role of cytokine licensing in shaping the therapeutic potential of wharton’s jelly MSCs: metabolic shift towards immunomodulation at the expense of differentiation by Olena Rogulska, Eliska Vavrinova, Irena Vackova, Jarmila Havelkova, Klara Gotvaldova, Pavel Abaffy, Sarka Kubinova, Michal Sima, Pavel Rossner, Lucie Bacakova, Pavla Jendelova, Katarina Smolkova, Yuriy Petrenko

“…Transcriptomic analysis was performed using RNA sequencing to identify differentially expressed genes related to immune modulation and lineage commitment. …”
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Intestinal inflammation and microbiota modulation impact cochlear function: emerging insights in gut-ear axis by Anna Pisani, Valentina Petito, Fabiola Paciello, Valeria Emoli, Letizia Masi, Veronica Mohamed Hizam, Pierluigi Puca, Raffaele Montuoro, Federica Del Chierico, Lorenza Putignani, Claudio Grassi, Jacopo Galli, Maurizio Taglialatela, Maria Emiliana Caristo, Gianluca Ianiro, Loris Riccardo Lopetuso, Giovanni Cammarota, Antonio Gasbarrini, Anna Rita Fetoni, Franco Scaldaferri

“…Donor microbiota composition was characterized by 16S rRNA sequencing, and systemic inflammation was evaluated by measuring serum lipopolysaccharide (LPS) levels. …”
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Incorporating Differential Gene Expression Analysis with Predictive Biomarkers to Identify Novel Therapeutic Drugs for Fuchs Endothelial Corneal Dystrophy by Huaming Wen, Ryan A. Gallo, Xiaosheng Huang, Jiamin Cai, Shaoyi Mei, Ammad Ahmad Farooqi, Jun Zhao, Wensi Tao

“…Based on the differential gene expression analysis for predictive biomarkers with RNA-Sequencing data from Fuchs endothelial corneal dystrophy (FECD) patients, we are aiming to evaluate the efficacy of Library of Integrated Network-based Cellular Signatures (LINCS) perturbagen prediction software to identify novel pharmacotherapeutic targets that can revert the pathogenic gene expression signatures and reverse disease phenotype in FECD. …”
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Assessing metabarcoding-based identifications for monitoring beetle communities in temperate forests by R․G․Bina Perl, Alexander Schneider, Julio V. Schneider, Viktor Hartung, Angela Röhner, Sebastian Will, Steffen U. Pauls

“…With the advent of high-throughput sequencing technologies, DNA metabarcoding has evolved into a particularly useful tool for speedy identification of species from bulk samples. …”
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Surveillance of single nucleotide polymorphisms correlated to macrocyclic lactone resistance in Dirofilaria immitis from client-owned dogs across the United States by Emily Curry, David Tack, Jessica Rodriguez, Danielle Brehm-Lowe, John Letherer, Megan Lineberry, Roger Prichard, Tobias Clark

“…Through opportunistic sampling, genotypically and phenotypically confirmed ML-resistant D. immitis isolates have been isolated in the Lower Mississippi River Valley region (LMRV); however, the pervasiveness of resistant isolates in the USA has not been evaluated. This study aimed to evaluate the geographic distribution and prevalence of genotypically ML-resistant heartworms in client-owned dogs across the USA over a 3-year period. …”
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3D cryo-printed hierarchical porous scaffolds provide immobilization of surface-functionalized sleep-inspired small extracellular vesicles: synergistic therapeutic strategies for v... by Xu-Ran Li, Qing-Song Deng, Shu-Hang He, Po-Lin Liu, Yuan Gao, Zhan-Ying Wei, Chang-Ru Zhang, Fei Wang, Tong-He Zhu, Helen Dawes, Bi-Yu Rui, Shi-Cong Tao, Shang-Chun Guo

“…The hierarchical pore size distribution of the scaffolds was evaluated by scanning electron microscopy (SEM) and micro-computed tomography (micro-CT). …”
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Correlation analysis of circulating PCDH17 DNA methylation changes with rheumatoid arthritis patients by Fuyu Zhao, Mi Zhou, Jia Liu, Cen Chang, Ping Jiang, Kai Wei, Jianan Zhao, Yu Shan, Yixin Zheng, Yiming Shi, Yunshen Li, Yuejuan Zheng, Qianqian Li, Lei Wang, Huanru Qu, Liangjing Lv, Shicheng Guo, Xinliang Lv, Qi Zhu, Dongyi He

“…Peripheral blood samples were collected from a cohort of 370 individuals, comprising patients diagnosed with RA, ankylosing spondylitis (AS), psoriatic arthritis (PsA), gout, systemic lupus erythematosus (SLE), dermatomyositis (DM), primary Sjögren’s syndrome (SS), and healthy controls (HC), for subsequent analysis.DNA methylation sequencing techniques were employed to evaluate the methylation levels of the PCDH17 cg03865667 locus. …”
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Prevalence and pathogenic potential of Shewanella species in oysters and seawater collected from the Chesapeake Bay and Maryland Coastal Bays by Tahirah Johnson, Gary P. Richards, John Jacobs, Howard Townsend, Esam Almuhaideb, Detbra Rosales, Paulinus Chigbu, Ligia Dasilva, Salina Parveen

“…Shewanella is a genus of Gram-negative marine bacteria with some species associated with human and shellfish illnesses. This study evaluated the abundance of Shewanella species in oysters and seawater from the Chesapeake and Maryland Coastal Bays at four sites between 2019 and 2021. …”
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