Ribosome engineering of Myxococcus xanthus for enhancing the heterologous production of epothilones by Xu Kang, Xiao-ran Yue, Chen-xi Wang, Jia-rui Wang, Jun-ning Zhao, Zhao-peng Yang, Qin-ke Fu, Chang-sheng Wu, Wei Hu, Yue-zhong Li, Xin-jing Yue

“…Results Antibiotics that target the ribosome and RNA polymerase (RNAP) were evaluated for ribosome engineering of the epothilone-producing strain M. xanthus ZE9. …”
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Therapeutic effect of fecal microbiota transplantation on hyperuricemia mice by improving gut microbiota by Songjian Yuan, Wenting Jia, Xiaomei Liu, Ruzhen Liu, Man Cao, Yuting Wu, Yuantao Li, Wei Xu, Chuanxing Xiao, Chuanxing Xiao, Zhenqiang Hong, Bangzhou Zhang, Bangzhou Zhang

“…Regular body weight checks, serum biochemical analyses, and fecal sampling for 16S rRNA gene sequencing were conducted to evaluate the treatment’s impact on gut microbiota.ResultsThe model group showed significant increases in uric acid (UA), creatinine (Cr), blood urea nitrogen (BUN) levels, and increased xanthine oxidase (XOD) activity compared to controls (p < 0.05). …”
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The Usefulness of the BD MAX MDR-TB Molecular Test in the Rapid Diagnosis of Multidrug-Resistant Tuberculosis by Tomasz Bogiel, Edyta Dolska, Małgorzata Zimna, Kornelia Nakonowska, Dorota Krawiecka, Renata Żebracka, Maciej Pochowski, Agnieszka Krawczyk

“…Complementary tests like Xpert MTB/XDR or whole-genome sequencing could improve diagnostic accuracy and support more effective TB control strategies.…”
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Effect of Raspberry (<i>Rubus indeaus</i> L.) Juice Fermented by <i>Limosilactobacillus fermentum</i> FUA033 on the Human Gut Microbiota Cultured In Vitro: A Multi-Omics Approach by Ziyan Hua, Yunfan Lv, Han Zhang, Tianyi Mao, Ruyu Xv, Mingxuan Pan, Yadong Hu, Shu Liu, Yaowei Fang

“…We performed 16S rRNA gene sequencing and nontargeted metabolomics analyses to evaluate changes in the microbial composition and metabolite profiles resulting from fermentation. …”
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Comprehensive analysis of the oral microbiota and metabolome change in patients of burning mouth syndrome with psychiatric symptoms by Shihong Luo, Fangzhi Lou, Li Yan, Yunmei Dong, Yingying Zhang, Yang Liu, Ping Ji, Xin Jin

“…The aims of this study were to investigate the potential interplay between psychiatric symptoms and BMS.Methods Using 16S rRNA sequencing and liquid chromatography-mass spectrometry (LC/MS) to evaluate the oral microbiota and saliva metabolism of 40 BMS patients [including 29 BMS patients with depression or anxiety symptoms (DBMS)] and 40 age matched healthy control (HC).Results The oral microbiota composition in BMS exhibited no significant differences from HC, although DBMS manifested decreased α-diversity relative to HC. …”
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Genetic basis of pulmonary arterial hypertension: a prospective study from a highly inbred population by Abdullah M. Aldalaan, Khushnooda Ramzan, Sarfraz A. Saleemi, Ihab Weheba, Laila Alquait, Abeer Abdelsayed, Fatima Alzubi, Hamdeia Zaytoun, Nadeen Alharbi, Mohammed Al‐Owain, Faiqa Imtiaz

“…This study aims to identify genetic variations and to evaluate the frequency of risk genes associated to PAH, in Saudi Arabian patients. …”
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Machine learning based on pangenome-wide association studies reveals the impact of host source on the zoonotic potential of closely related bacterial pathogens by Cheng Han, Shiying Lu, Pan Hu, Jiang Chang, Deying Zou, Feng Li, Yansong Li, Qiang Lu, Honglin Ren

“…Our results present an open pangenome of Brucella spp. derived from the whole-genome sequencing (WGS) data of 991 strains and identify 268 genes potentially associated with the zoonotic potential of Brucella. …”
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Methylation Status of the Telomerase Reverse Transcriptase Promoter in Parotid Tumours and Adjacent Parotid Gland Tissue: A Pilot Study on the Implications for Recurrence and Devel... by António Paiva-Correia, Joana Apolónio, Alfons Nadal, José Ricardo Brandão, Nádia Silva, Bianca Machado, Ivan Archilla, Pedro Castelo-Branco, Henrik Hellquist

“…This pilot study aimed to evaluate THOR methylation as a potential biomarker for recurrence/malignant transformation in salivary gland pleomorphic adenomas (PA). …”
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miR-183-5p Is a Potential Molecular Marker of Systemic Lupus Erythematosus by Shaolan Zhou, Jing Zhang, Pengfei Luan, Zhanbing Ma, Jie Dang, Hong Zhu, Qian Ma, Yanfeng Wang, Zhenghao Huo

“…Methods. Next-generation sequencing (NGS) was performed to assess miRNA amounts in peripheral blood mononuclear cells (PBMCs) from four SLE cases and four healthy controls. …”
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ROLE OF MAGNETIC RESONANCE IMAGING IN THE STUDY OF PATIENTS WITH DIABETIC FOOT SYNDROME by M. A. Zamyshevskaya, V. D. Zavadovskaya, V. D. Udodov, M. A. Zorkal’tsev, E. G. Grigor’ev

“…Ankle joint and foot MRI was carried out in T1-weighted MR image, T2-weighed MRI image, and FSat sequences. The soft tissue, tendoligamentous apparatus, and bones were evaluated. …”
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Correlation between Microbial Community Composition and Characteristic Flavor Substances in Stinky Mandarin Fish during Low-Temperature Fermentation by ZHOU Yingqin, HUANG Jingjing, LUO Gege, CHENG Aiwu, YAN Yan, XIE Ningning

“…We undertook this work in order to study the correlation between the microbial community composition and the characteristic flavor substances in stinky mandarin fish during the low-temperature fermentation process. 16S rDNA high-throughput sequencing was used to determine the microbial community composition. …”
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Adaptive Blind Stokes-Space Based Equalizer for RSOP in SV-DD Systems With High Chromatic Dispersion Tolerance by Yu Jin, Di Li, Pin Yi, Mengfan Cheng, Songnian Fu, Ming Tang, Deming Liu, Lei Deng

“…In our simulation, the conventional Stokes space-based method and the training sequences (TS) method are both evaluated for comparison. …”
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Methimazole disrupted skeletal ossification and muscle fiber transition in Bufo gargarizans larvae by Fei Wei, Xueyi Wu, Hongyuan Wang, Yongpu Zhang, Lei Xie

“…Muscle sarcomere was elongated in both the MMI and T4 treatment groups, which may lead to muscle weakness and consequently affect land motion. Additionally, we evaluated the expression levels of fast muscle-related genes (TNNI2 and TNNT3) and slow muscle-related genes (TNNI1 and TNNT1), revealing an opposite trend in the transition from fast to slow muscle after T4 and MMI exposures. …”
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Plant-derived nanovesicles as novel nanotherapeutics for alleviating endothelial cell senescence-associated vascular remodeling induced by hypertension by Fengxia Lin, Luhua Xu, Qiuting He, Zetao Chen, Weiwei Zhang, Jin Tu, Yinzhi Song, Fanjia Zhong, Sheng Lin, Rongfeng Yang, Zhicong Zeng

“…In vitro, SDNVs inhibited angiotensin II-induced senescence in human umbilical vein endothelial cells (HUVECs). miRNA and mRNA sequencing revealed that SDNVs downregulate CD38 expression through miR393a delivery, mediating their anti-senescence effects. …”
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Association between the PLTP rs4810479 SNP and Serum Lipid Traits in the Chinese Maonan and Han Populations by Fen-Han Zhang, Rui-Xing Yin, Li-Mei Yao, Wei-Xiong Lin, Jin-Zhen Wu, De-Zhai Yang

“…This investigation aimed to evaluate the relationship between the PLTP rs4810479 SNP, several environmental risk factors, and serum lipid parameters in the Chinese Maonan and Han nationalities. …”
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Predominant yeasts in Chinese Dong fermented pork (Nanx Wudl) and their aroma-producing properties in fermented sausage condition by Ruifang Mi, Xi Chen, Suyue Xiong, Biao Qi, Jiapeng Li, Xiaoling Qiao, Wenhua Chen, Chao Qu, Shouwei Wang

“…In this study, yeast communities of Chinese Dong fermented pork (Nanx Wudl) were investigated and the technological properties of 73 yeast isolates were evaluated. Through culture-dependent and high-throughput sequencing methods, the main yeast species identified included Pichia membranifaciens, Kazachstania bulderi, Millerozyma farinosa, Candida zeylanoides, Kazachstania exigua, Candida parapsilosis and Saccharomyces cerevisiae. …”
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Topical application of the HSP90 inhibitor 17-AAG reduces skin inflammation and partially restores microbial balance: implications for atopic dermatitis therapy by Krzysztof Sitko, Ewa Piotrowska, Magdalena Podlacha, Natalia Zagórska, Michał D. Starke, Magdalena Trzeciak, Stefan Tukaj

“…Here the effects of the HSP90 inhibitor 17-AAG applied topically were evaluated in a DNCB-induced murine model of atopic dermatitis (AD). …”
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L-theanine promotes angiogenesis in limb ischemic mice by modulating NRP1/VEGFR2 signaling by Jingyi Wang, Yinghui Xu, Yating Ruan, Xinyang Hu

“…This study explores the pro-angiogenic effects of L-theanine and its underlying mechanisms in a mouse model of hindlimb ischemia (HLI). To evaluate L-theanine's pro-angiogenic effects, human umbilical vein endothelial cells (HUVECs) were subjected to tube formation, migration, sprouting, and proliferation assays. …”
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Detecting gene copy number alterations by Oncomine Comprehensive genomic profiling in a comparative study on FFPE tumor samples by Fabio Bozzi, Elena Conca, Marco Silvestri, Gianpaolo Dagrada, Alice Ardore, Donata Penso, Daniele Lorenzini, Chiara Costanza Volpi, Desirè Viola Trupia, Adele Busico, Iolanda Capone, Federica Perrone, Elena Tamborini, Andrea Vingiani, Luca Agnelli, Giancarlo Pruneri

“…The introduction of other procedures, such as Next-Generation Sequencing has dramatically improved CNAs discovery at genome-wide level. …”
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Profiling of driver mutations in lung adenocarcinoma patients identifies rare compound EGFR mutations sensitive to second-generation EGFR-TKIs by Jun Li, Cuiyun Zhang, Yuping Guan, Siyu Wang, Jiawen Zheng, Junnan Feng, Sile Han, Ruijuan Ma, Pengfei Ren, Shasha Li, Harry J. M. Groen, Klaas Kok, Anke van den Berg, Bing Wei, Jie Ma, Hongle Li, Yongjun Guo

“…Tyrosine kinase inhibitors (TKIs) are standard care for the patients with these mutations. In this study, we evaluated the efficiency of an NGS-based 8-gene test in selecting TKIs-sensitive patients in a cohort of treatment-naive Chinese LUAD patients and evaluated the sensitivity of rare compound mutations to different EGFR-TKIs in vitro. …”
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