Ras and Rab interactor 3 as a prognostic biomarker and its impact on immune cell infiltration in cancer by Xin Xu, Ningfeng Zhou, Shasha Zhang, Tingxin Cheng, Jun Ren, Dian Yang, Ying Liu, Shiyan Wang, Aijun Sun, Fengze Cai

“…We evaluated the association between RIN3 levels and patient survival via univariate Cox regression and analyzed its correlation with immune cell infiltration using TIMER2.0. …”
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Gut microbial communities in chronic rhinosinusitis patients in response to 1,8-Cineol treatment by Simon Graspeuntner, Mathias Heidemann, Stephanie Jeschke, Mariia Lupatsii, Zuzana Penxova, Sven Künzel, Barbara Wollenberg, Anke Leichtle, Michael Ploch, Karl-Ludwig Bruchhage, Ralph Pries

“…Methods: Microbiome analysis from stool samples was performed from 31 CRS patients before and after 1,8-Cineol administration using 16S rRNA gene next generation sequencing using dissimilarity-based and taxonomic approaches. …”
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Genetic variation and structure shaped by recent population fragmentation in the boreal conifer Thuja koraiensis: Conservation perspectives by Eun-Kyeong Han, Ichiro Tamaki, Tae-Im Heo, Jun-Gi Byeon, Amarsanaa Gantsetseg, Young-Jong Jang, Jong-Soo Park, Jung-Hyun Lee

“…We used a genome dataset (242 SNPs) generated by the MIG-seq (Multiplexed ISSR Genotyping by Sequencing) method to evaluate the genetic diversity and structure of T. koraiensis populations across their entire distribution range. …”
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Using molecular transmission networks to understand the epidemic characteristics of HIV-1 CRF08_BC across China by Kang Li, Meiliang Liu, Huanhuan Chen, Jianjun Li, Yanling Liang, Yi Feng, Hui Xing, Yiming Shao

“…HIV-1 CRF08_BC has become a major epidemic in heterosexuals and intravenous drug users (IDUs) in southern China. In order to evaluate the trends of its epidemic and facilitate targeted HIV prevention, we constructed the genetic transmission networks based on its pol sequences, derived from the National HIV Molecular Epidemiology Survey. …”
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Safety and efficacy of genomic biomarker-guided neoadjuvant therapy for locally advanced and oligometastatic prostate cancer (SEGNO): study protocol for an open-label prospective p... by Haichao Huang, Tao Wang, Wei Li, Zhun Wu, Xuegang Wang, Jinchun Xing, Bin Chen, Kaiyan Zhang

“…Methods In this open-label prospective phase II umbrella clinical trial, 40 patients will be enrolled. Next-generation sequencing data analysis of PCa tissues from the diagnostic needle biopsies will be performed. …”
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Mucosal washes are useful for sampling intestinal mucus-associated microbiota despite low biomass by Jennifer N. Martinez-Medina, Fereshteh Ghazisaeedi, Catharina Kramer, Jörn F Ziegler, Victoria McParland, Paul W. Mönch, Britta Siegmund, Víctor Hugo Jarquín-Díaz, Marcus Fulde, Sofia K. Forslund-Startceva

“…We used DNA quantification and 16S rRNA amplicon sequencing to evaluate the comparability of the information yielded from the different sample types under a controlled benchmark. …”
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Sustainable Modular Adaptive Redundancy Technique Emphasizing Partial Reconfiguration for Reduced Power Consumption by R. Al-Haddad, R. Oreifej, R. A. Ashraf, R. F. DeMara

“…The software layer supervises the organic activities on the FPGA and extends the self-healing capabilities through application-independent, intrinsic, and evolutionary repair techniques that leverage the benefits of dynamic partial reconfiguration (PR). SMART was evaluated using a Sobel edge-detection application and was shown to tolerate stressful sequences of injected transient and permanent faults while reducing dynamic power consumption by 30% compared to conventional triple modular redundancy (TMR) techniques, with nominal impact on the fault-tolerance capabilities. …”
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The molecular composition of soil organic matter is regulated by bacterial community under biochar application by Zongkun Yang, Wenbo Liu, Xiaoge Fan, Han Gao, Xiangrui Xu, Cheng Liu, Yanjun Chai, Min Zhang, Marios Drosos, Shengdao Shan

“…However, changes in SOM molecular composition following biochar application and their interactions with the soil bacterial communities remain poorly understood. We aimed to evaluate SOM molecular composition, soil bacterial communities, and carbon cycle functional genes of bacteria in soils treated with biochar using pyrolysis–gas chromatography-mass spectrometry (py-GC/MS) and amplicon sequencing. …”
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Identification of common mutations and their frequency in METTL5, METTL7A, and METTL7B genes in gastrointestinal cancers based on in silico data by Soraya Heydari, Maryam Peymani, Mehrdad Hashemi, Kamran Ghaedi, Maliheh Entezari

“…Methods: We utilized DNA sequencing data available for each sample in the TCGA database to identify common mutations and their frequencies in the candidate genes. …”
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Effect of <i>Elaeagnus angustifolia</i> Honey in the Protection Against Ethanol-Induced Chronic Gastric Injury via Counteracting Oxidative Stress, Interfering with Inflammation and... by Min Zhu, Jiayan Yang, Haoan Zhao, Yu Qiu, Lin Yuan, Jingyang Hong, Wei Cao

“…Moreover, gastric ulcer indices, histopathological morphology, oxidative stress markers (MDA, GSH, SOD), inflammatory mediators (NO, PGE2), and cytokine gene expression (TNF-α, IL-6, IL-1β, iNOS) were evaluated via enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR. …”
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Development of a LAMP method for detection of carbapenem-resistant Acinetobacter baumannii during a hospital outbreak by Carolina Garciglia Mercado, Ramon Gaxiola Robles, Felipe Ascencio, Jesus Silva-Sanchez, Maria Teresa Estrada-Garcia, Gracia Gomez-Anduro

“…Methodology: A set of six primers were designed for recognizing eight distinct sequences on six targets: blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, blaOXA-58-like, blaIMP, and blaVIM. …”
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Developing molecular surveillance of SARS-CoV-2 in the Czech Republic (2021–2022) by Timotej Šúri, Lucie Pfeiferová, Matěj Bezdíček, Jan Svatoň, Vladimír Hampl, Karel Berka, Helena Jiřincová, Martina Lengerová, Martin Kolísko, Alexander Nagy, Ruth Tachezy, Czech COVID-19 Genomics Consortium, Michal Kolář, Jan Pačes

“…In the Czech Republic, molecular surveillance employed whole genome sequencing (WGS) and variant discrimination polymerase chain reaction (VD-PCR) on samples collected through passive, active and sentinel surveillance. …”
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Multi-species probiotic supplement enhances vagal nerve function – results of a randomized controlled trial in patients with depression and healthy controls by Sabrina Mörkl, Martin Narrath, Daria Schlotmann, Marie-Therese Sallmutter, Julia Putz, Julia Lang, Andreas Brandstätter, Rene Pilz, Helmut Karl Lackner, Nandu Goswami, Bianca Steuber, Jasmin Tatzer, Sonja Lackner, Sandra Holasek, Annamaria Painold, Emanuel Jauk, Julian Wenninger, Angela Horvath, Nicolai Spicher, Asmus Barth, Mary I Butler, Jolana Wagner-Skacel

“…Vagus nerve (VN) function was evaluated using 24-hour electrocardiography (ECG) for heart rate variability (HRV), alongside stool microbiome analysis via 16S rRNA sequencing. …”
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Gamma -aminobutyric acid ameliorates neurological impairments in type 1 diabetes mellitus mice by regulating the “gut flora-LPS-TLR4-NF-ΚB” signalling Axis by Jiao Wang, Lihai Zhang, Xianhe Wang, Jing Dong, Jiaxin Li

“…The study employed flow cytometry to analyze colonic Th17/Treg cells, 16 S rRNA sequencing to analyze microbiota, and western blot to evaluate colonic proteins. …”
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Optical Flow Magnification and Cosine Similarity Feature Fusion Network for Micro-Expression Recognition by Heyou Chang, Jiazheng Yang, Kai Huang, Wei Xu, Jian Zhang, Hao Zheng

“…A novel multi-channel feature fusion module leverages cosine similarity between Query and Key token sequences to optimize feature integration. Extensive evaluations on four public datasets—CASME II, SAMM, SMIC-HS, and MMEW—demonstrate MCNet’s superior performance, achieving state-of-the-art results with 92.88% UF1 and 86.30% UAR on the composite dataset, surpassing the best prior method by 1.77% in UF1 and 6.0% in UAR.…”
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Design and Immune Profile of Multi-Epitope Synthetic Antigen Vaccine Against SARS-CoV-2: An In Silico and In Vivo Approach by Maria da Conceição Viana Invenção, Larissa Silva de Macêdo, Ingrid Andrêssa de Moura, Lucas Alexandre Barbosa de Oliveira Santos, Benigno Cristofer Flores Espinoza, Samara Sousa de Pinho, Lígia Rosa Sales Leal, Daffany Luana dos Santos, Bianca de França São Marcos, Carolina Elsztein, Georon Ferreira de Sousa, Guilherme Antonio de Souza-Silva, Bárbara Rafaela da Silva Barros, Leonardo Carvalho de Oliveira Cruz, Julliano Matheus de Lima Maux, Jacinto da Costa Silva Neto, Cristiane Moutinho Lagos de Melo, Anna Jéssica Duarte Silva, Marcus Vinicius de Aragão Batista, Antonio Carlos de Freitas

“…This construct was evaluated in vivo as a DNA vaccine and elicited T CD4+ and T CD8+ cell expansion in the blood and spleen. …”
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Resources for innovative learning of anatomy and foot ossification: Graphic design and virtual reality by Ana Cristina Moreno‐Marin, Manuel Pardo Rios, Eva Lopezosa‐Reca, Cristina Molina García, Salvador Díaz‐Miguel, Beatriz Gómez‐Martín, Paula Cobos‐Moreno

“…Understanding the chronology of foot bone formation is essential for evaluating pathological processes and establishing appropriate therapeutic actions to improve patient quality of life. …”
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Liver macrophage-derived exosomal miRNA-342-3p promotes liver fibrosis by inhibiting HPCAL1 in stellate cells by Wenshuai Li, Lirong Chen, Qi Zhou, Tiansheng Huang, Wanwei Zheng, Feifei Luo, Zhong Guang Luo, Jun Zhang, Jie Liu

“…Method Exosomes were extracted from primary mouse macrophages and cultured with HSCs, and the differential expression of microRNAs was evaluated using high-throughput sequencing technology. …”
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Gut Microbiota-Targeted Intervention of Hyperlipidemia Using <i>Monascus</i>-Fermented Ginseng by Qing Zhou, Cuiting Yang, Mingyue Jia, Qingsong Qu, Xinhui Peng, Weishuo Ren, Guoqing Li, Yueyang Xie, Bingxuan Li, Xinyuan Shi

“…<b>Methods:</b> The MFG-modulated gut microbiota was transplanted into HLP mice. Systemic evaluations, including serum biochemical parameter detection, histopathological section analysis, 16S rRNA sequencing, and fecal metabolomics, were conducted to assess therapeutic efficacy and identify associated metabolic pathways. …”
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Estradiol Reverses Ovariectomy-Induced Disruption of Hypothalamic Gene Expression and Behavior via Modulation of Gonadotropin Releasing Hormone and Calcium Signaling Pathways by Asim Muhammad, Mubashir Muhammad, Xiaohuan Chao, Chunlei Zhang, Jiahao Chen, Huan Yang, Shuhan Liu, Yuan Ding, Ziming Wang, Hongwei Bi, Wen Guo, Junhong Fan, Bo Zhou

“…This study investigates the transcriptional and behavioral changes induced by ovariectomy (OVX) and estradiol (E2) supplementation in female C57BL/6J mice. RNA sequencing was performed to identify differentially expressed genes (DEGs) across control (CK), E2, OVX, and OVX+E2 groups, followed by functional enrichment and pathway analyses. …”
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