Autoantibodies to harmonin and villin are diagnostic markers in children with IPEX syndrome. by Vito Lampasona, Laura Passerini, Federica Barzaghi, Carlo Lombardoni, Elena Bazzigaluppi, Cristina Brigatti, Rosa Bacchetta, Emanuele Bosi

“…Autoantibodies to enterocyte antigens harmonin (75 kDa USH1C protein) and villin (actin-binding 95 kDa protein) are associated with the Immune dysregulation, Polyendocrinopathy, Enteropathy, X-linked (IPEX) syndrome. In this study we evaluated the diagnostic value of harmonin and villin autoantibodies in IPEX and IPEX-like syndromes. …”
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Geometric Effects on Nanopore Creation in Graphene and on the Impact-withstanding Efficiency of Graphene Nanosheets by Sadegh Sadeghzadeh

“…These sheets are used in a variety of applications, including DNA sequencing, water desalination, and phase separation. …”
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Effect of reductive soil disinfestation on melon fusarium wilt and soil metabolomics by Xuelian Ren, Xiaodi Liu, Zhaoran Zhang, Jialin Shao, Zenggui Gao, Yufei Huang

“…We designed a potted RSD treatments system to grow melons and applied it to the greenhouse. By evaluating its impacts on melon Fusarium wilt disease occurrence and soil metabolites composition using survey statistics and soil metabolomics sequencing analysis. …”
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Machine learning for predicting Plasmodium liver stage development in vitro using microscopy imaging by Corin F. Otesteanu, Reto Caldelari, Volker Heussler, Raphael Sznitman

“…We collected and analyzed hourly imaging data over a span of at least 38 hours from 400 sequences, encompassing 502 parasites. Our method was compared to human annotations to validate its efficacy. …”
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In Silico Proteomic Profiling and Bioactive Peptide Potential of Rapeseed Meal by Katarzyna Garbacz, Jacek Wawrzykowski, Michał Czelej, Adam Waśko

“…Among the evaluated enzymes, bromelain exhibited the highest efficacy, yielding the greatest quantity and diversity of bioactive peptides. …”
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Study of the Carrier State for Five BRCA1/BRCA2 Deleterious Mutations in Bulgarian Women with Breast Cancer by Katia Kovacheva, Zornica Kamburova, Savelina Popovska, Ivan Ivanov, Maria Simeonova, Petia Angelova

“…They were screened for five deleterious mutations: 5382insC in BRCA1 and 6174delT, 6079del4, 8138del5, 5946delCT in BRCA2, by DNA sequencing. The genetic analysis detected none of the tested mutations. …”
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Transcriptomics profiling of Parkinson’s disease progression subtypes reveals distinctive patterns of gene expression by Carlo Fabrizio, Andrea Termine, Carlo Caltagirone

“…Methods Whole blood RNA-Sequencing data underwent differential gene expression analysis, followed by multiple pathway analyses. …”
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Potential role of the skin and gut microbiota in premenarchal vulvar lichen sclerosus: A pilot case-control study. by Suhana Chattopadhyay, Justin D Arnold, Leena Malayil, Lauren Hittle, Emmanuel F Mongodin, Kalyani S Marathe, Veronica Gomez-Lobo, Amy R Sapkota

“…Future studies involving larger samples sizes are warranted to further evaluate this association.…”
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Phylogenetic and functional assessment of orthologs inference projects and methods. by Adrian M Altenhoff, Christophe Dessimoz

“…This required the mapping of millions of sequences, the handling of hundreds of millions of predicted pairs of orthologs, and the computation of tens of thousands of trees. …”
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Protective effects of Atractylodes macrocephala polysaccharides on acetaminophen-induced liver injury by Jiali Wu, Biao Jia, Shuai Gong, Yangpeng Li, Jiaqi Wang, Yuqiao Huang, Jiao Guo

“…Mice were pretreated with AMPs for 7 days prior to APAP challenge, and liver injury was evaluated through histopathology, serum biochemistry, molecular assays, and gut microbiota analysis.ResultsAMPs treatment significantly reduced serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels compared to the APAP group (p < 0.05). …”
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The Critique of Margin Call’s Film with Ethical Decision-Making in Critical Situations by Mohammad Reza Hamidizadeh, Fatemeh Ordoo

“…The purpose of this article is to criticize Margin Call’s film content and evaluate screenplay sequences, actors’ dialogues, organizational behavior, and other management topics based on the right and ethical decision making of managers in critical situations. …”
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Impacts of host phylogeny, diet, and geography on the gut microbiome of rodents. by Sufia Akter Neha, John D Hanson, Jeremy E Wilkinson, Robert D Bradley, Caleb D Phillips

“…The present study focused on 12 species of rodents representing 3 rodent families and 3 dietary guilds (herbivores, granivores, and omnivores) to evaluate the influence of host phylogeny, dietary guild and geography on microbial diversity and community composition. …”
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Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts by Bae Seunghee, An In-Sook, An Sungkwan

“…In the second step, we validated certain effective reagents identified in the first step by analyzing the cell cycle, evaluating cell death, and performing HPRT-DNA sequencing in DREAM-F cells treated with these reagents and UVB. …”
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Liver quad culture chip as a model for radiation injury research by Yuki Ueda, Satoshi Omiya, Jonathan Pinney, Michelle A. Bylicky, Molykutty J. Aryankalayil

“…These findings highlight the potential of the liver quad-culture chip as an effective model for investigating the microenvironment in RILD and for evaluating the efficacy of therapeutic countermeasures and biomarkers.…”
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Galactose‐α‐1,3‐galactose‐presenting bacterial families are associated with resistance to experimental avian malaria infection by R. K. Noble, T. R. Kelly, C. R. Lattin

“…Before and after inoculation, we collected blood samples to quantify circulating parasitemia and fecal samples to evaluate the gut microbiome through amplification and sequencing of the V4 region of the bacterial 16S rRNA gene. …”
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Elevated CD14 in B cells associates with reduced ovarian cancer risk via CD80 + dendritic cell interaction: a multi-omics study by Mengyu Zheng

“…Methods This study employed single-cell RNA sequencing (scRNA-seq) to analyze peripheral blood mononuclear cells (PBMCs) from ovarian cancer patients and healthy donors. …”
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Engineering, decoding and systems-level characterization of chimpanzee cytomegalovirus. by Quang Vinh Phan, Boris Bogdanow, Emanuel Wyler, Markus Landthaler, Fan Liu, Christian Hagemeier, Lüder Wiebusch

“…To re-evaluate the CCMV coding potential, we analyzed the viral transcriptome and proteome and identified several novel ORFs, splice variants, and regulatory RNAs. …”
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DNA repair and replicative stress addiction in neuroblastoma by Kaat Durinck, Meredith S. Irwin

“…While several effective chemotherapies work by increasing replication stress in cancer cells, recently, newer putatively less toxic small molecule-based approaches that directly target DNA damage response (DDR) pathway components such as ATR, CHK1 and PARP inhibitors are being evaluated in early phase trials for many cancers. NB sequencing studies have identified recurrent alterations (copy number and mutations) in many of these genes encoding critical DDR pathway proteins suggesting susceptibility to specific classes of DDR-targeting therapies. …”
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Targeting mitophagy in diabetic retinopathy: novel insights into SQSTM1/BNIP3L pathway regulated by luteolin by Shuyan Zhang, Jiajun Wu, Yinjian Zhang

“…This study aimed to explore the local and systemic immunological mechanisms underlying luteolin’s therapeutic effects on DR.MethodsKey regulatory genes and cell subpopulations were identified from single-cell RNA sequencing (scRNA-Seq) datasets derived from peripheral blood mononuclear cells (PBMCs) and retinal tissues of DR patients. …”
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Cloning, expression, and purification of recombinant AKR1D1 for therapeutic applications by Shutevska Kristina, Nestorovska Aleksandra Kapedanovska

“…We identified optimal conditions for ligation and precise DNA sequencing, emphasizing the need for lower DNA concentrations and higher purity. …”
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