Analysis of gene polymorphisms in patients with pulmonary infections based on next-generation sequencing technology and their prognostic predictive value by Rui Zeng, Guang Wang, Feng Zhou

“…This study utilized next-generation sequencing (NGS) technology to analyze gene polymorphisms in 200 patients with pulmonary infections and evaluate their prognostic value. …”
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Analysis by next-generation sequencing of bacterial communities in Japanese cedar logs buried for 2 years for ground improvement by Sitompul Afrida, Sakae Horisawa, Tadashi Hara

“…Soil environmental factors such as pH, total carbon, total nitrogen, and the C:N ratio were measured to evaluate their influence on bacterial community structure. …”
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Assessment of Ply Stacking Sequence Effect on Damage Behavior of CFRP Composite Laminate Under Low-Velocity Impacts by Murlidhar Patel, Lokesh Sonkar, Shivdayal Patel

“…To guarantee that the laminate can sustain the projected impact energy, the impact load should be carefully evaluated during the design phase. To obtain an optimized stacking layup orientation, the damage behaviors of excellent specific strength carbon fiber–reinforced polymer (CFRP) composite laminates with stacking sequences of [0°]8 (unidirectional), [0°/90°]2s (cross-ply), [0°/+45°/−45°/90°]s (quasi-isotropic), and [0°/−30°/−60°/−90°/90°/60°/30°/0°] (antisymmetric) were numerically investigated under LVI. …”
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Comprehensive Sequencing of Environmental RNA From Japanese Medaka at Various Size Fractions and Comparison With Skin Swab RNA by Kyoshiro Hiki, Toshiaki S. Jo

“…In this study, we aimed to determine the optimal filter pore size range for eRNA analysis and evaluate the contribution of skin and mucus to eRNA profiles. …”
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Impact of selected non-steroidal anti-inflammatory pharmaceuticals on microbial community assembly and activity in sequencing batch reactors. by Cong Jiang, Jinju Geng, Haidong Hu, Haijun Ma, Xingsheng Gao, Hongqiang Ren

“…The objective is to evaluate the impact of NSAIDs at their environmental concentrations on microbial community assembly and activity. …”
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Benchmarking of bioinformatics tools for the hybrid de novo assembly of human and non-human whole-genome sequencing data by Adrián Muñoz-Barrera, Luis A. Rubio-Rodríguez, David Jáspez, Almudena Corrales, Itahisa Marcelino-Rodriguez, Lourdes Ortiz, Pablo Mendoza, José M. Lorenzo-Salazar, Rafaela González-Montelongo, Carlos Flores

“…However, the literature has limited comprehensive evaluations of software performance, especially for human genome assembly. …”
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Genetic Diversity of Olive (<i>Olea europaea</i> L.) Cultivars Assessed by Genotyping-by-Sequencing in Southern Peru by Martín Eloy Casilla García, Rina Alvarez Becerra, José Cotrado Cotrado, Juan Iván Casilla Rondán, Janet Libertad Huatuco Coaquira, Edgar Virgilio Bedoya Justo

“…This study aimed to evaluate the morphological and genomic diversity of ten economically important olive varieties cultivated in 15 sectors across Tacna and Jorge Basadre provinces. …”
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Accuracy of next-generation sequencing for rapid diagnosis of tuberculous pleurisy: A protocol of systematic review and meta-analysis. by Yuyang Ling, Yanqin Shen, Xudong Xu, Guocan Yu

“…<h4>Background</h4>Early, rapid and precise diagnosis of tuberculous pleurisy is difficult. Next-generation sequencing (NGS) has been used for the diagnosis of TB in some high-income countries. …”
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EST–SSR Marker Development and Full-Length Transcriptome Sequence Analysis of Tiger Lily (Lilium lancifolium Thunb) by Mingwei Sun, Yilian Zhao, Xiaobin Shao, Jintao Ge, Xueyan Tang, Pengbo Zhu, Jiangying Wang, Tongli Zhao

“…The fast advancement and deployment of sequencing technologies after the Human Genome Project have greatly increased our knowledge of the eukaryotic genome sequences. …”
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Whole genome sequencing reveals a de novo SHANK3 mutation in familial autism spectrum disorder. by Sergio I Nemirovsky, Marta Córdoba, Jonathan J Zaiat, Sabrina P Completa, Patricia A Vega, Dolores González-Morón, Nancy M Medina, Mónica Fabbro, Soledad Romero, Bianca Brun, Santiago Revale, María Florencia Ogara, Adali Pecci, Marcelo Marti, Martin Vazquez, Adrián Turjanski, Marcelo A Kauffman

“…Here we present three siblings with ASD where we evaluated the usefulness of Whole Genome Sequencing (WGS) for the diagnostic approach to ASD.…”
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Optimizing genomic diversity assessments for conservation of Bromus auleticus (Trinius ex Nees) using individual and pooled sequencing. by Luciana Gillman, Federico Condón, Cesar Petroli, Mercedes Rivas

“…Therefore, it is essential to assess appropriate methodologies for developing population genomic studies that will contribute to the conservation of this genetic resource. In this study, we evaluated five accessions using two genotyping strategies: individual sequencing (ind-seq) and pooled sequencing (pool-seq). …”
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Mutational spectrum of breast cancer by shallow whole-genome sequencing of cfDNA and tumor gene panel analysis. by Fernando Ambriz-Barrera, Ernesto Rojas-Jiménez, Clara Estela Díaz-Velásquez, Aldo Hugo De-La-Cruz-Montoya, Héctor Martínez-Gregorio, Miguel Ruiz-De-La-Cruz, Antonio Huertas, Ana Lorena Montealegre, Carlos Castro-Rojas, Gabriela Acosta, Felipe Vaca-Paniagua, Sandra Perdomo

“…We evaluated the feasibility to detect somatic copy number alterations (SCNAs) in BC using shallow whole genome sequencing (sWGS) in cfDNA from archived samples from National Cancer Institute of Colombia patients. …”
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PtWAVE: a high-sensitive deconvolution software of sequencing trace for the detection of large indels in genome editing by Kazuki Nakamae, Saya Ide, Nagaki Ohnuki, Yoshiko Nakagawa, Keisuke Okuhara, Hidemasa Bono

“…PtWAVE also offers options for variable selection and fitting algorithms to prevent uncertainties in the model. We evaluated the performance of PtWAVE by using in vitro capillary sequencing data that mimicked DNA sequencing, including large deletions. …”
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Application of rapid clinical exome sequencing technology in the diagnosis of critically ill pediatric patients with suspected genetic diseases by Xuejun Ouyang, Dazhi Chi, Yu Zhang, Tian Yu, Qian Zhang, Lei Xu, Victor Wei Zhang, Bin Wang

“…PurposeThis study evaluates the efficacy of rapid clinical exome sequencing (CES) and mitochondrial DNA (mtDNA) sequencing for diagnosing genetic disorders in critically ill pediatric patients.MethodsA multi-centre investigation was conducted, enrolling critically ill pediatric patients suspected of having genetic disorders from March 2019 to December 2020. …”
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Central position of histidine in the sequence of designed alternating polarity peptides enhances pH-responsive assembly with DNA by Razieh Taghizadeh Pirposhteh, Nasir Mohajel, Arash Arashkia, Kayhan Azadmanesh, Mohammadali Masoumi

“…Abstract Background Self-assembling peptides hold great promise for medical applications, particularly as carriers for gene delivery, but their potential remains unrealized due to a limited understanding of how amino acid sequence positioning affects their properties. In this study, we designed and evaluated two alternating polarity peptides, RFH (RFRHRHRFR) and RHF (RHRFRFRHR), differing only in the position of their histidine and phenylalanine residues, to investigate the impact of sequence variation on pH-responsive DNA co-assembly and transfection efficiency. …”
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PMSFF: Improved Protein Binding Residues Prediction through Multi-Scale Sequence-Based Feature Fusion Strategy by Yuguang Li, Xiaofei Nan, Shoutao Zhang, Qinglei Zhou, Shuai Lu, Zhen Tian

“…Additionally, the proposed model treats protein sequences as sentences, employing a bidirectional GRU to learn global context. …”
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Genome sequencing and comparison of five Tilletia species to identify candidate genes for the detection of regulated species infecting wheat by Hai D. T. Nguyen, Tahera Sultana, Prasad Kesanakurti, Sarah Hambleton

“…Of the DNA-based methods published so far, most have focused on sequence variation among tested strains at a single gene locus. …”
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Nocardia Detection in Respiratory Samples Using Targeted Next-Generation Sequencing: Cautious Interpretation Is Necessary for Timely Discovery by Chaowen Deng, Simon Kam-Fai Lo, Kelvin Hei-Yeung Chiu, Fanfan Xing

“…The expanding application of targeted next-generation sequencing (tNGS) in clinical diagnostics has led to an increasing frequency of Nocardia spp. detection in respiratory specimens; however, the clinical relevance and interpretation of these findings remain uncertain. …”
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Diagnostic Comparison of MRI Sequences for Assessing Myometrial Invasion in Endometrial Cancer: A 1.5T MRI Study by Abdusselim Adil Peker, Ahmet Akçay

“…Conclusion: All three magnetic resonance sequences demonstrated high diagnostic accuracy for evaluating myometrial invasion in EC. …”
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Intercellular Communication Network of CellChat Uncovers Mechanisms of Kidney Fibrosis Based on Single-Cell RNA Sequencing by Lei Lei, Yun-Xiu Xiang, Mao-Lin Luo, Ze-Yu Zhang, Hong-Wei Wu, Chun Tang, Tian-Jiao Cui, Xue-Mei Zhang, Xiao-Hua Wang, Denis Delic, Thomas Klein, Yvonne Liu, Bernhard K. Krämer, Zhi-Hua Zheng, Yong-Ping Lu, Berthold Hocher, Ting Zhu

“…Kidney function was assessed by measuring blood urea nitrogen, serum creatinine, and urinary albumin-to-creatinine ratio. Renal fibrosis was evaluated histologically. Single-cell RNA sequencing (scRNA-seq) was performed to analyze intercellular communication networks and identify alterations in ligand-receptor pairs and signaling pathways involved in fibrosis. …”
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