Good performance of monoclonal antibody-based stool H. pylori antigen tests in acute nonvariceal upper gastrointestinal bleeding patients by Yu-Li Song, Jin-Ru Wang, Jian-Ping Zhang, Jun-Hua Weng, Da-Yu Kuai, Bao-Hong Xu

“…Fecal HP nucleic acid detection by nested PCR combined with Sanger sequencing was used as the gold standard. Results In total, 82 patients with ANVUGIB were included. …”
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Relieving Effect of <i>Artemisia ordosica Krasch</i> Extract on DSS-Induced Colitis by Regulating Immunity, Antioxidant Function, Gut Microbiota, and Bile Acid Metabolism in Mice by Min Jiang, Xuekai Zhang, Xiao Jin, Binlin Shi, Yuanqing Xu, Zheqi Wang

“…The protective effect of AOE on colitis in mice was determined by monitoring key indicators such as body weight, colon length, and survival rate in mice, as well as by assessing the expression of colon-related genes and cytokine levels. We evaluated the impact of AOE on intestinal microbiota by measuring the 16s sequencing of cecal contents and bile acid metabolism. …”
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Isolation and characterization of bacteriophages with lytic activity against multidrug-resistant non-typhoidal Salmonella from Nairobi City county, Kenya by Michael Mugo, Abednego Musyoki, Angela Makumi, Ivy Mutai, Kelvin Kering, Peter Muturi, Collins Kebenei, Kristin Weber, Michael Pietsch, Tanja Pilz, Oliver Drechsel, Tobias Hoffmann, Lothar Wieler, Cecilia Mbae, Antje Flieger, Samuel Kariuki

“…Phage genomes were sequenced using the Illumina sequencing platform, and analyzed with bioinformatics tools to screen for antimicrobial resistance (AMR), lysogeny, virulence, and allergenic genes. …”
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Oral PD-L1 inhibitor GS-4224 selectively engages PD-L1 high cells and elicits pharmacodynamic responses in patients with advanced solid tumors by Nicholas Waldron, P Rod Dunbar, Sanjeev Deva, Oh Kyu Yoon, John Ling, Kai-Wen Lin, Dung Thai, Latesh Lad, Ahmed A Othman, Jared M Odegard, Adele Y Wang, Jonathan Nazareno, Edmond Ang

“…Biomarkers evaluated included target occupancy, flow cytometric immunophenotyping, plasma cytokine measurements, and T-cell receptor sequencing.Results GS-4224 binding caused dimerization of PD-L1, blocking its interaction with PD-1 and leading to reversal of T-cell inhibition and increased tumor killing in vitro and in vivo. …”
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Profiling of Bacterial Communities of Hospital Wastewater Reveals Clinically Relevant Genera and Antimicrobial Resistance Genes by Clemente Cruz-Cruz, Javier Gaytán-Cervantes, Carolina González-Torres, Andres Emmanuel Nolasco-Rojas, Miguel Ángel Loyola-Cruz, Laura Delgado-Balbuena, Josué Delgado-Balbuena, Marianela Paredes-Mendoza, María Concepción Tamayo-Ordóñez, Yahaira de Jesús Tamayo-Ordoñez, Emilio Mariano Durán-Manuel, Araceli Rojas-Bernabé, Carlos Alberto Jiménez-Zamarripa, Oscar Sosa-Hernández, Omar Agni García-Hernández, Esther Ocharan-Hernández, Paola Berenice Zárate-Segura, Elizabeth González-Terreros, Daniel Alejandro Ramírez-Villanueva, Claudia Camelia Calzada-Mendoza, Juan Manuel Bello-López

“…Fourteen physicochemical parameters were determined by using standard methodologies, and statistical differences between discharges and seasons were evaluated. Bacterial communities were analysed by targeted amplicon sequencing of the V3-V4 region of the <i>16S rRNA</i> gene. …”
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A comparative Analysis by SAGE of Gene Expression Profiles of Esophageal Adenocarcinoma and Esophageal Squamous Cell Carcinoma by Jantine W. P. M. van Baal, Francesco Milana, Agnieszka M. Rygiel, Carine M. T. Sondermeijer, C. Arnold Spek, Jacques J. G. H. M. Bergman, Maikel P. Peppelenbosch, Kausilia K. Krishnadath

“…Over 58,000 tags were sequenced. Between EA and BE 1013, and between ESCC and normal squamous epithelium 1235 tags were significantly differentially expressed (p < 0.05). …”
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Type-2 diabetes epigenetic biomarkers: present status and future directions for global and Indigenous health by Sarah Munns, Sarah Munns, Alex Brown, Alex Brown, Sam Buckberry, Sam Buckberry

“…In this review, we summarise DNA methylation profiling methods, and discuss how these technologies can facilitate the discovery of epigenomic biomarkers for Type-2 diabetes. We critically evaluate previous DNA methylation biomarker studies, particularly those using microarray platforms, and advocate for a shift towards sequencing-based approaches to improve genome-wide coverage. …”
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Influence of SCD and FABP3 genetic markers on carcass traits and meat quality in Aberdeen Angus bulls by Sena Ardicli, Nursen Senturk, Tugce Necla Selvi, Sena Yonga, Rumeysa Celik, Beyza Karalar, Aleyna Ozdeniz, Madina Aliyeva, Havvanur Aydin, Ozgur Toprak Ay, Ozge Ardicli, Muhammed Emin Sari, Berkay Bozkurt, Oliva Giannelli Viscardi, Huseyn Babayev, Hakan Ustuner

“…ABSTRACT This study evaluated the association of stearoyl-CoA desaturase (SCD) and fatty acid-binding protein 3 (FABP3) gene polymorphisms with carcass and meat quality traits in Aberdeen Angus bulls. …”
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Role of permafrost thaw transitions in biogeochemical nitrogen cycling by Merritt N. Logan, Monique S. Patzner, Jacob P. VanderRoest, Bridget B. McGivern, Nivetha Srikanthan, Myrna J. Simpson, Amy M. McKenna, Kelly C. Wrighton, Casey Bryce, Andreas Kappler, Thomas Borch

“…In addition, we performed metatranscriptomic sequencing to evaluate microbial activity changes in N cycling pathways between the palsa and bog. …”
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A Comparison of Two Different Methods for Inducing Apnoea During Thoracic Computed Tomography of Dogs by Thomas Hordle, Maria Navarro-Carrillo, Imogen Schofield, Mark Plested, Maria Chie Niimura del Barrio

“…Therefore, the purpose of this study was to evaluate the efficacy of two different methods used to induce apnoea. …”
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Insecticide resistance status and high frequency of kdr mutations in Aedes aegypti in Tegucigalpa, Honduras by Cindy Reyes-Perdomo, Denis Escobar, Luis Galo, Oscar Urrutia, Rita Lucrecia Vizcaino, Audrey Lenhart, Gustavo Fontecha

“…Susceptibility to four insecticides was evaluated via bioassays. Frequencies of the F1534C and V1016I kdr alleles and their haplotypes were determined, and sequencing of the vgsc gene was performed for further genotyping. …”
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Exploring the Root-Associated Bacterial Community of Tomato Plants in Response to Salt Stress by Antonia Esposito, Valeria Scala, Francesco Vitali, Marzia Beccaccioli, Massimo Reverberi, Giuseppe Valboa, Sara Del Duca, Loredana Canfora, Stefano Mocali

“…To understand its adaptation to soil salinization, the microbiome was studied by total DNA extraction and sequencing, using the Illumina MiSeq platform. Additionally, we evaluated the plant metabolites associated with salt stress (oxylipins, fatty acids (FAs) and hormones) by mass spectrometry. …”
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A base editing platform for the correction of cancer driver mutations unmasks conserved p53 transcription programs by Pascal Wang, Rituparno Sen, Frank Buchholz, Shady Sayed

“…Using this approach, we correct TP53 hotspot mutations in cancer cell lines derived from diverse tissues, followed by mRNA sequencing to evaluate transcriptional changes. Remarkably, correcting these mutations not only reveals the dependency on mutant allele expression but also restores highly conserved tumor-suppressive transcriptional programs, irrespective of tissue origin or co-occurring mutations, highlighting a shared p53-dependent regulatory network. …”
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Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis by Jingyao Chen, Jingyao Chen, Shumin Cheng, Liang Gu, Liang Gu, Zhangsen Huang, Zhangsen Huang, Changhua Zhang, Changhua Zhang, Chunhui Sun, Chunhui Sun, Chunhui Sun, Songyao Chen, Songyao Chen

“…HE staining, immunohistochemistry, and DNA sequencing were utilized to compare the biological characteristics between the original tumor and the organoid. …”
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Characterization of microbial biofilms on marine plastics: community development and density implications for vertical transport dynamics by Gregory M. Joern, Rut Pedrosa-Pàmies, Xiangtao Jiang, Zhanfei Liu, Alexander B. Bochdansky

“…By examining the accumulation of biogenic silica on plastic surfaces, we evaluated the potential role of microbial biofilms in influencing plastic sinking behavior in the water column. …”
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Meloidogyne marylandi is Involved in, but not the Primary Cause of Creeping Bentgrass Decline of Putting Greens in Southern California by Ploeg A. T., Witte H., Subbotin S. A., Tandingan De Ley I., Smith Becker J., Becker J. O.

“…This identification was confirmed by analysis of the D2-D3 expansion segments of 28S rRNA and COI gene sequences. The host status of 28 plant species was evaluated in greenhouse trials. …”
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Application and mechanism of anticancer peptides in organoid models of intrahepatic cholangiocarcinoma by Xuekai Hu, Yue Zhang, Yanchen Li, Qiuxia Zheng, Haixia Zhao, Yun Zhang, Jingman Ni, Jia Yao

“…In this study, organoids can be used as an in vitro model to evaluate the therapeutic response of ACPs and offer novel insight for the study of ICC.…”
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Two-Stage Video Violence Detection Framework Using GMFlow and CBAM-Enhanced ResNet3D by Mohamed Mahmoud, Bilel Yagoub, Mostafa Farouk Senussi, Mahmoud Abdalla, Mahmoud Salaheldin Kasem, Hyun-Soo Kang

“…This paper proposes a two-stage framework for detecting violent actions in video sequences. The first stage leverages GMFlow, a pre-trained optical flow network, to capture the temporal motion between consecutive frames, effectively encoding motion dynamics. …”
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HPD-Kit: a comprehensive toolkit for pathogen detection and analysis by Tengcheng Que, Tengcheng Que, Tengcheng Que, Wen Li, Wen Li, Wen Li, Zhining Zhang, Yunlin He, Kangming He, Hong Qiu, Hong Qiu, Juan Huang, Zhiwei Lu, Chunlan Jiang, Yongjian Huang, Hui Huang, Qiuyu Wu, Panyu Chen, Yanling Hu, Yanling Hu, Yanling Hu, Wenjian Liu

“…IntroductionUnbiased metagenomic sequencing (mNGS) is crucial for infectious disease diagnosis and epidemiological surveillance. …”
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Molecular Diagnostics, Targeted Therapy, and the Indication for Allogeneic Stem Cell Transplantation in Acute Lymphoblastic Leukemia by Anthony Oyekunle, Torsten Haferlach, Nicolaus Kröger, Evgeny Klyuchnikov, Axel Rolf Zander, Susanne Schnittger, Ulrike Bacher

“…Furthermore, the potential of high-throughput sequencing should be evaluated for diagnosis and follow-up of patients with B-lineage ALL.…”
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