Pond Water eDNA Reflects Broad Consistency with Surrounding Terrestrial Plant Ecosystems by Duygu Bozdogan, Shogo Takizawa, Norihiro Furukori, Kosuke Homma, Harue Abe, Hitoshi Sakio, Naoki Harada, Kazuki Suzuki

“…This study evaluates the potential of using pond water eDNA to reflect the surrounding terrestrial plant communities, aiming to develop a sustainable, large-scale, and long-term monitoring method for plant diversity in forest ecosystems. …”
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Landscape of extracellular small RNA and identification of biomarkers in multiple human cancers by Shoubin Zhan, Ye Xu, Botao Li, Peng Ge, Chunwu Zhang, Shengkai Zhou, Tingting Yang, Gaoli Liang, Ling Ji, Xiangbin Kong, Ping Yang, Xi Chen, Chen-Yu Zhang, Han Shen, Xu Luo, Zhen Zhou, Yanbo Wang

“…Small non-coding RNAs, such as rsRNAs and tsRNAs, are abundant in tissues and likely secreted into biofluids, contributing to exRNA profiles. To comprehensively evaluate exRNAs, we employed traditional and enzymatic treatment RNA sequencing to systematically profile exRNAs across six human biofluids including serum, ascites, urine, milk, seminal plasma and saliva as well as sera from mice, rats, rabbits, and bovines. rsRNAs were identified as the most abundant exRNA species in human biofluids, with rsRNAs and tsRNAs showing high expression and species-specific profiles across animals. …”
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Presence of viable gram-positive bacteria in blood of patients with inflammatory bowel disease is not affected by treatment by Sanna Davidson, Yunjeong So, Elin Oscarsson, Åsa Håkansson, Klas Sjöberg

“…Identification of viable bacteria in the blood was evaluated by optimized cultivation and Sanger sequencing and for quantification real-time PCR was performed. …”
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ASVBM: Structural variant benchmarking with local joint analysis for multiple callsets by Peizheng Mu, Xiangyan Feng, Lanxin Tong, Jie Huang, Chaoqun Zhu, Fei Wang, Wei Quan, Yuanjun Ma, Yucui Dong, Xiao Zhu

“…Accurate benchmarking of structural variant (SV) detection is essential for advancing the development and application of human whole-genome sequencing (WGS). A fundamental challenge in benchmarking SV detection results is determining whether two SVs represent the same event. …”
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Excretion of Host DNA in Feces Is Associated with Risk of Clostridium difficile Infection by Caroline Vincent, Sudeep Mehrotra, Vivian G. Loo, Ken Dewar, Amee R. Manges

“…Whole-genome shotgun sequencing was employed to quantify host DNA excretion and evaluate bacterial content in fecal samples collected from patients with incipient CDI, hospitalized controls, and healthy subjects. …”
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Decoding Quantitative Traits in Yaks: Genomic Insights for Improved Breeding Strategies by Yujiao Fu, Yuanyuan Yu, Xinjia Yan, Daoliang Lan, Jiabo Wang

“…This review explores the application and potential of molecular marker-assisted selection (MAS) and genomic prediction (GP) in yak genetic improvement. We systematically evaluate critical components of genomic breeding pipelines, including: (1) phenotypic trait assessment, (2) sample collection strategies, (3) reference population design, (4) high-throughput genotyping (via genome sequencing and SNP arrays), (5) predictive model development, and (6) heritability estimation. …”
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The Endophytic Bacterial Microbiota Associated with Sweet Sorghum (Sorghum bicolor) Is Modulated by the Application of Chemical N Fertilizer to the Field by Cintia Mareque, Thais Freitas da Silva, Renata Estebanez Vollú, Martín Beracochea, Lucy Seldin, Federico Battistoni

“…PCR-DGGE, quantitative PCR, and high-throughput sequencing were performed based on the amplification of rrs and nifH genes. …”
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Integrated analysis of blood microbiome and metabolites reveals key biomarkers and functional pathways in myocardial infarction by Ikram Khan, Stefan Panaiotov, Kotb A. Attia, Arif Ahmed Mohammed, Muhammad Uzair, Imran Khan, Zhiqiang Li, Xiaodong Xie

“…Methods Using 16 S rRNA sequencing and LC-MS metabolomics, blood samples from 24 MI patients and 24 healthy controls were analyzed. …”
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Isolation and Characterization of Biosurfactant-Producing Bacteria from Amapaense Amazon Soils by Elisa Maria de Oliveira, Victor Hugo Gomes Sales, Marcelo Silva Andrade, Jerri Édson Zilli, Wardsson Lustrino Borges, Tiago Marcolino de Souza

“…The isolates were cultured in nutrient broth with olive oil, and their extracts were evaluated according to drop collapse, oil dispersion, emulsification, and surface tension tests. …”
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Population Genetics of Atlantic Salmon (Salmo salar) in Prince Edward Island, Canada by Carissa M. Grove, Scott D. Roloson, Kyle M. Knysh, Scott A. Pavey, David K. Cairns, Robert F. Gilmour Jr., Michael R. van denHeuvel

“…This study used a panel of six microsatellites and next‐generation sequencing to evaluate the genetic composition of 884 individuals from 20 rivers. …”
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Forced intracellular degradation of xenoantigens as a modality for cell-based cancer immunotherapy by Jean Pierre Bikorimana, Roudy Farah, Jamilah Abusarah, Gabrielle Arona Mandl, Mohamed Ali Erregragui, Marina Pereira Gonçalves, Sebastien Talbot, Perla Matar, Malak Lahrichi, Nehme El-Hachem, Moutih Rafei

“…Summary: Given recent leverage of mesenchymal stromal cells (MSCs) as a potent vaccination platform, we investigated whether forced degradation of an expressed experimental antigen fused to small degron sequences could prime potent antitumoral responses. …”
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Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer by Tatsunori Minamide, Nobuhisa Minakata, Riu Yamashita, Shingo Sakashita, Yusuke Yoda, Akihiro Ohashi, Masato Aoshima, Susumu Kobayashi, Tomonori Yano

“…Therefore, this exploratory study aimed to evaluate the biological implications of oxygen saturation imaging and further assess tumor heterogeneity in gastric cancer. …”
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Glucose deprivation and identification of TXNIP as an immunometabolic modulator of T cell activation in cancer by Agathe Dubuisson, Adèle Mangelinck, Samantha Knockaert, Adrien Zichi, Etienne Becht, Wendy Philippon, Sandra Dromaint-Catesson, Manon Fasquel, Fabien Melchiore, Nicolas Provost, Dawid Walas, Dawid Walas, Hélène Darville, Jean-Pierre Galizzi, Céline Lefebvre, Véronique Blanc, Vincent Lombardi

“…In cancer, the tumor microenvironment (TME) represents a complex system with a strong metabolism stress, in part due to glucose shortage, which limits proper T cell activation, differentiation and functions preventing anti-tumor immunity.MethodsIn this study, we evaluated T cell immune reactivity in glucose-restricted mixed lymphocyte reaction (MLR), using a comprehensive profiling of soluble factors, multiparametric flow cytometry and single cell RNA sequencing (scRNA-seq).ResultsWe determined that glucose restriction potentiates anti-PD-1 immune responses and identified thioredoxin-interacting protein (TXNIP), a negative regulator of glucose uptake, as a potential immunometabolic modulator of T cell activation. …”
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Bats are a potential reservoir of pathogenic Leptospira species in Colombia by Jose Mateus, Natalia Gómez, María Teresa Herrera-Sepúlveda, Marylin Hidalgo, Jairo Pérez-Torres, Claudia Cuervo

“…The aim of this study was to evaluate the presence of pathogenic Leptospira spp. in the kidneys of bats from the Caribbean region of Colombia deposited in the collection of mammals of the Museo Javeriano de Historia Natural (MPUJ-MAMM). …”
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Enhancing Integrated Navigation with a Self-Attention LSTM Hybrid Network for UAVs in GNSS-Denied Environments by Ziyi Wang, Xiaojun Shen, Jie Li, Juan Li, Xueyong Wu, Yu Yang

“…The performance of the proposed method is evaluated using flight data obtained from field tests. …”
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Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability by Nallely Espinoza, Edward Spinard, Ayushi Rai, Elizabeth Ramirez-Medina, Alyssa Valladares, Amanda Meyers, Manuel V. Borca, Douglas P. Gladue

“…Recently a vaccine, ASFV-G-ΔI177L was approved for use in Vietnam. In this study we evaluate the long-term stability of lyophilized ASFV-G-ΔI177L. …”
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Intrusion Alert Analysis Method for Power Information Communication Networks Based on Data Processing Units by Rui Zhang, Mingxuan Zhang, Yan Liu, Zhiyi Li, Weiwei Miao, Sujie Shao

“…Finally, the suffix tree models attack activities, capturing key behavioral paths of high-severity alerts and identifying attacker patterns. Experimental evaluations on the CPTC-2017 and CPTC-2018 datasets validate the proposed method’s effectiveness in reducing alert redundancy, extracting critical attack behaviors, and constructing attack activity sequences. …”
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ACACA depletion activates the cPLA2–arachidonic acid–NF-κB axis to drive inflammatory reprogramming in androgen receptor-independent prostate cancer by Shaoyou Liu, Yupeng Chen, Jian Chen, Jinchuang Li, Zhenguo Liang, Xinyue Mei, Yuanfa Feng, Zhaodong Han, Funeng Jiang, Yongding Wu, Huijing Tan, Hongwei Luo, Huichan He, Jiarun Lai, Weide Zhong

“…Transcriptomic, metabolomic, and single-cell RNA sequencing data were used to identify downstream changes. …”
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A platform for multisite immune profiling of premetastatic pancreatic cancer at single-cell resolution by Elishama N. Kanu, Ashley A. Fletcher, Jiayin Bao, Ethan S. Agritelley, Julia Button, Austin M. Eckhoff, Karrie Comatas, Tao Wang, Bin-Jin Hwang, Michael E. Lidsky, Sabino Zani, Dan G. Blazer, Peter J. Allen, Zhicheng Ji, Frank J. Lowery, Sri Krishna, Nicholas D. Klemen, Daniel P. Nussbaum, Erika J. Crosby

“…Derived immune cells underwent paired single-cell RNA and TCR sequencing. Immune composition, cell-type functional profiles, and T cell clonal expansion patterns were evaluated across tissue sites. …”
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Inhibition of SIRT3 by a specific inhibitor induces cellular senescence and growth arrest of ovarian granulosa cell tumor via p53 and NF-κB axis by Jingxin Ma, Sailing Lin, Chuimian Zeng, Wenhao Wu, Qi Zhang, Guli Zhu, Qi Zhang, Qiongfang Fang, Lijun Fan, Shunichi Takeda, Xiaoyu Li, Xiaoyu Li, Xiushen Li, Yu Zhou, Xueqing Wu

“…RNA sequencing and gene set enrichment analyses revealed that 77–39 led to the activation of the p53 and NF-κB signaling pathways, which were confirmed by Western blot assay. …”
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