AXL promotes inflammatory breast cancer progression by regulating immunosuppressive macrophage polarization by Lan T. H. Phi, Yating Cheng, Yohei Funakoshi, Francois Bertucci, Pascal Finetti, Steven J. Van Laere, Fang Zou, James P. Long, Suguru Ogata, Savitri Krishnamurthy, James M. Reuben, Jason M. Foulks, Steven L. Warner, Jennifer M. Rosenbluth, Anil K. Sood, Debu Tripathy, Naoto T. Ueno, Xiaoping Wang

“…Additionally, we knocked out AXL expression in human THP-1 monocytes and evaluated the effect of AXL signaling on immunosuppressive M2 macrophage polarization and IBC cell growth and migration. …”
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Adipsin improves diabetic hindlimb ischemia through SERPINE1 dependent angiogenesis by Xiaohua Zhang, Mengyuan Jiang, Xuebin Zhang, Yixuan Zuo, Huanle Zhang, Tingting Zhang, Liyu Yang, Jie Lin, Yan Zhang, Xinchun Dai, Wen Ge, Chuang Sun, Fang Yang, Jiye Zhang, Yue Liu, Yangyang Wang, Huanhuan Qiang, Xiaojie Yang, Dongdong Sun

“…This study aimed to evaluate the possible therapeutic effect of Adipsin in hind-limb ischemia in type 2 diabetic mice and to elucidate the molecular mechanisms involved. …”
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Early-onset enriches the identification of actionable alterations in patients with KRAS wild-type pancreatic ductal adenocarcinoma by E. Scarlato, S. Casalino, L. Mendo, A. Sordo, V. De Vita, E. San Lorenzo, A. Quinzii, C. Zecchetto, G. Butturini, D. Melisi

“…Results: Next-generation sequencing was carried out on 224 PDAC and 10 pancreatic acinar cell carcinoma (PACC) patients. …”
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Comparative effects of raw and processed cistanche glycosides on the HPT axis and gut microbiota in a rat model of kidney-yang deficiency by Xiaoqing Shen, Jing Lian, Chao Zhang, Yixiang Miu, Yuan Zhang, Ji Shi, Ji Shi, Ji Shi, Nan Xu, Nan Xu, Nan Xu, Tianzhu Jia, Tianzhu Jia, Tianzhu Jia

“…However, the precise interactions between gut microbiota and KYD, as well as the mechanisms of raw and processed CD total glycosides (CDG) in modulating KYD, require further investigation. This study aims to evaluate the effects and mechanisms of CDG in a KYD rat model using 16S rRNA gene sequencing and fecal metabolomics.MethodsCDG was extracted from both raw and processed CD and analyzed via HPLC. …”
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Distinct immune responses to proton and photon radiotherapy: implications for anti-PD-L1 combination therapy in colorectal cancer by Hélène Burckel, Anaïs Nicol, Carole Mura, Marc Rousseau, Jolie Bou-Gharios, Lisa Froidurot, Corentin Richard, Véronique Morgand, Pierre-Antoine Laurent, Emeric Limagne, Romain Boidot, Georges Noël, Céline Mirjolet

“…Tumor growth was monitored to evaluate the efficacy of combining RT or PT with anti-PD-L1 IT. …”
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The therapeutic efficacy comparison of MSCs derived different tissues unveilings anti-apoptosis more crucial than angiogenesis in treating acute myocardial infarction by Mingjie Pan, Yueyue Xu, Yaping Wang, Yue Jiang, Yuanyuan Xie, Chenxu Tai, Wenqing Wang, Bin Wang

“…Additionally, the anti-apoptosis effects mediated by MSCs were further evaluated using flow cytometry in hypoxia H9C2 and HL-1 cells. …”
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Quercetin ameliorates obesity and inflammation via microbial metabolite indole-3-propionic acid in high fat diet-induced obese mice by Jiaxin Lu, Yanting Huang, Yujing Zhang, Jiayu Xie, Qingjun Guo, Huifan Yang, Yunyan Yang, Jing Chen, Lijie Su

“…In vitro experiments, Caco-2 cells were used to evaluate the effects of IPA and fecal dilutions from quercetin-treated mice on tight junction protein expression and aryl hydrocarbon receptor (AhR) activation.ResultsOur results revealed that quercetin supplementation significantly mitigated obesity and chronic inflammation, and improved the disrupted gut barrier function through the actvation of AhR/interleukin 22 (IL-22) pathway. 16S rRNA sequencing revealed that quercetin treatment increased the abundance of Lactobacillus. …”
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Sengers syndrome caused by biallelic TIMM29 variants and RNAi silencing in Drosophila orthologue recapitulates the human phenotype by Adel Shalata, Ann Saada, Mohammed Mahroum, Yarin Hadid, Chaya Furman, Zaher Eldin Shalata, Robert J. Desnick, Avraham Lorber, Asaad Khoury, Adnan Higazi, Avraham Shaag, Varda Barash, Ronen Spiegel, Euvgeni Vlodavsky, Pierre Rustin, Shmuel Pietrokovski, Irena Manov, Dan Gieger, Galit Tal, Adi Salzberg, Hanna Mandel

“…Linkage analysis, and sequencing were used to map and identify the disease-causing gene. …”
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A high content clonogenic survival drug screening identifies maytansine as a potent radiosensitizer for meningiomas by Jinxiu Yu, Jinxiu Yu, Jinxiu Yu, Jiaojiao Deng, Jiaojiao Deng, Jiaojiao Deng, Leihao Ren, Leihao Ren, Leihao Ren, Lingyang Hua, Lingyang Hua, Lingyang Hua, Tianqi Wu, Yi Hui, Chunlin Shao, Ye Gong, Ye Gong, Ye Gong, Ye Gong

“…Therefore, the aim of this study was to identify potential radiosensitizers for treating meningiomas.MethodsA high content clonogenic survival drug screening was employed to evaluate 166 FDA-approved compounds across varied concentration ranges. …”
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Adipose-derived stem cells extracellular vesicles enhance diabetic wound healing via CCN2/PI3K/AKT pathway: therapeutic potential and mechanistic insights by Yu-lu Zhou, Shingo Ogura, Hao Ma, Rong-bin Liang, Shao-yihan Fang, Yue-ming Wang, Yan Wo, Wen-jin Wang, De-Wu Liu

“…A diabetic rat wound model was used to evaluate their therapeutic impact on wound healing and angiogenesis, with histological and immunofluorescence analyses. mRNA sequencing identified Cellular communication network factor 2(CCN2) as a key upregulated gene, leading to further exploration of its role in ADSCs-EVs-mediated angiogenesis and wound healing via the PI3K/AKT pathway. …”
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Integrative analysis of taurine metabolism-related genes prognostic signature with immunotherapy and identification of ABCB1 and GORASP1 as key genes in nasopharyngeal carcinoma by Zhang Feng, Yuhang Yang, Wenqi Luo, Jinqing Li, Zhenlian Xie, Long Zuo, Meijiao Duan, Dongzhi Zuo, Ruwei MO, Xuejing Tang, Shijiang Yi, Xiaosong He, Fangxian Liu, Ning Ma, Feng He

“…Survival, medication sensitivity, and immunological microenvironment evaluations were performed to assess the prognostic utility of the model. …”
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Symbiotic bacteria associated with entomopathogenic nematodes showed molluscicidal activity against Biomphalaria glabrata, an intermediate host of Schistosoma mansoni by Jiranun Ardpairin, Chanakan Subkrasae, Abdulhakam Dumidae, Supawan Pansri, Chanatinat Homkaew, Wipanee Meesil, Tewarat Kumchantuek, Ittipon Phoungpetchara, Adler R. Dillman, Coralie Pavesi, Helge B. Bode, Sarunporn Tandhavanant, Aunchalee Thanwisai, Apichat Vitta

“…The aims of this study were to identify Xenorhabdus and Photorhabdus bacteria based on recA sequencing and evaluate their molluscicidal activity against B. glabrata snail. …”
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Fibrolamellar hepatocellular carcinoma: a case report and gene analysis by Akira Watanabe, Norifumi Harimoto, Hideyuki Saito, Reika Kawabata-Iwakawa, Takaomi Seki, Ryo Muranushi, Kouki Hoshino, Kei Hagiwara, Norihiro Ishii, Mariko Tsukagoshi, Takamichi Igarashi, Kenichiro Araki, Hayato Ikota, Takashi Ishige, Koshi Mimori, Ken Shirabe

“…We performed lymphadenectomy for the recurrent tumor. We performed RNA sequencing (RNA-seq) and targeted DNA sequencing of the resected specimens (primary tumor, lymphatic metastasis, and normal liver). …”
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Long-term prognosis of 47 pediatric patients with Blau syndrome in China by Xinwei Shi, Jianghong Deng, Junmei Zhang, Xiaozhen Zhao, Yinan Zhao, Li Li, Fengqiao Gao, Weiying Kuang, Jiang Wang, Xiaohua Tan, Chao Li, Shipeng Li, Caifeng Li

“…Direct sequencing of NOD2 gene was performed by sanger sequencing. …”
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Gasdermin D regulates the activation of EGFR in colorectal cancer by Ying Li, Jiayao Chen, Huijun Liang, Qindan Du, Jingjie Shen, Xiaoying Wang

“…GSDMD expression was evaluated by RT-qPCR, Western blot and immunohistochemical (IHC) analysis. …”
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Probiotic treatment induces sex-dependent neuroprotection and gut microbiome shifts after traumatic brain injury by Morgan Holcomb, Austin G. Marshall, Hannah Flinn, Mariana Lozano-Cavazos, Sirena Soriano, Fernando Gomez-Pinilla, Todd J. Treangen, Sonia Villapol

“…The taxonomic microbiome profiles of fecal samples were evaluated using 16S rRNA V1-V3 sequencing analysis, and Short-chain fatty acids (SCFAs) were measured. …”
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Comprehensive bioinformatics analysis identified HMGB3 as a promising immunotherapy target for glioblastoma multiforme by Libin Wang, Peizhi Xu, Xinglong Li, Qinghua Zhang

“…Bioinformatics analyses were carried to evaluate the functional of the high mobility group protein B3 (HMGB3) in GBM. …”
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Differential gene expression of heat shock protein in response to thermal stress, in two Fundulus species endemic to the Gulf of Mexico by Norberto Alonso Colín-García, Xavier Chiappa-Carrara, Jorge E. Campos, María Leticia Arena-Ortíz, Luis A. Hurtado

“…We analyze the variation in gene expression and genetic variation of 2 endemic Fundulus species in 2 lagoons to the north of the Yucatán Peninsula. We evaluated genetic distances between species using Hsp 70 gene sequences; our results showed low genetic differentiation between both Fundulus species and also showed individuals with high genetic variability, due to non-neutral polymorphisms. …”
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Learning-Enhanced Differential Evolution for Multi-Mode Resource-Constrained Multi-Project Scheduling Problem in Industrial Prefabrication by Zijie Xing, Chen Chen, Robert Lee Kong Tiong

“…Evaluated on real-world production data from the PBU industry, the algorithm produces high-quality solutions with strong scalability. …”
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The use of transcriptomics in situ study through fish: a systematic review on pollution by Jessica Cardenas-Camacho, Ivonne Calderón-Delgado, Wilson Corredor-Santamaría, Yohana M. Velasco-Santamaría

“…In this sense, fish become the main axis to evaluate the quality of ecosystems through increasingly detailed tools such as RNA sequencing (RNA-seq) to identify metabolic pathways and genes. …”
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