Osimertinib for Uncommon Epidermal Growth Factor Receptor-Mutant Non-Small Cell Lung Carcinoma: A Case Report by Niveditha Popuri, Vishnu Nagalapuram, Unaiza Zaman, Raid Aljumaily

“…Tumor tissue next-generation sequencing showed EGFR exon 19 mutation (DNA change: c.2237_2255delinsT, amino acid change: p.E746_S752delinsV [Glu 746_Ser752delinsval]). …”
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Dual inhibition of GTP-bound KRAS and mTOR in lung adenocarcinoma and squamous cell carcinoma harboring KRAS G12C by Masaoki Ito, Yoshihiro Miyata, Shoko Hirano, Nagisa Morihara, Misako Takemoto, Fumiko Irisuna, Kei Kushitani, Kenichi Suda, Junichi Soh, Yukio Takeshima, Yasuhiro Tsutani, Morihito Okada

“…We explored alternative changes in genetic pathways and evaluated the effectiveness of combination therapy using several types of cell lines and KRAS inhibitors. …”
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HCAR2 Exerts Anti-Depressive Effects on Corticosterone-Induced Depression in Mice by Modulating Microglial Activity by Zhao Pan, Li Jiang, Jiacheng Chen, Sicong Xu, Ping Zhang, Yili Yi, Yangzhi Xie, Yongjun Chen

“…Methods: We established a mouse model of corticosterone (Cor)-induced depression to evaluate the therapeutic potential of HCAR2 activation on depression. …”
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Clinical implement of Probe‐Capture Metagenomics in sepsis patients: A multicentre and prospective study by Qin Sun, Ran Teng, Qiankun Shi, Yun Liu, Xing Cai, Bin Yang, Quan Cao, Chang Shu, Xu Mei, Weiqi Zeng, Bingxue Hu, Junyi Zhang, Haibo Qiu, Ling Liu

“…This study, for the first time, evaluates the clinical impact of metagenomic sequencing by integrating antibiotic adjustments and SOFA score changes, indicating that approximately one‐fifth of sepsis patients benefit from this advanced diagnostic approach. …”
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IGF2BP2 binding to CPSF6 facilitates m6A‐mediated alternative polyadenylation of PUM2 and promotes malignant progression in ovarian cancer by Xin Luo, Qinglv Wei, Lingcui Xie, Ningxuan Chen, Bin Gu, Jiani Xu, Xiaoyan Jiang, Xinzhao Zuo, Hongyan Zhao, Xiaoyi Liu, Yu Yang, Tao Liu, Yong Zhu, Ping Yi, Jing Xu

“…Methods The correlation between m6A and APA was analyzed by using RNA methylation sequencing of OC cells and single‐cell sequencing of clinical samples from public databases. …”
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UBE2J1 is identified as a novel plasma cell-related gene involved in the prognosis of high-grade serous ovarian cancer by Yunjie Tian, Ruoyu Dong, Yingxia Guan, Ying Wang, Wei Zhao, Jun Zhang, Shan Kang

“…Methods We utilized single-cell sequencing data from the Gene Expression Omnibus (GEO) database to identify key immune cell types within HGSOC tissues and to extract related markers via the Seurat package. …”
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YTHDF3 regulates IL32 mRNA stability to promote osteogenic differentiation of bone mesenchymal stem cells in ankylosing spondylitis by Bole Zhou, Xinzhe Feng, Changhao Han, Yang Wu, Junjie Qiao, Wenwen Tong, Weidong Xu

“…This work employed quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blot (WB) analysis, and enzyme-linked immunosorbent assay to determine the expression of osteogenic markers, including RUNX2, SP7, BMP2, and OCN. Furthermore, RNA sequencing (RNA-seq), m6A sequencing (m6A-seq), and RNA immunoprecipitation were performed to identify downstream targets, particularly focusing interleukin 32 (IL32). …”
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3T BOLD and DW magnetic resonance imaging quantitative parameters for prediction of early diabetic nephropathy by Omnia Ezz Eldin, Yahya Bashir Ali, Noha Mohamed Elkholy, Yasser Mazloum, Ashraf Naguib Ettaby

“…Conclusions BOLD and DWI MRI sequences show promise in evaluating renal function in diabetic patients. …”
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Effects of Periodic Short-Term Heat Stress on Biological Characteristics and Gut Bacteria of <i>Spodoptera frugiperda</i> by Jingjing Jia, Min Liang, Zhitao Zhao, Weikang Huang, Qing Feng, Zhufeng Lin, Xuncong Ji

“…Combined with 16S rRNA high-throughput sequencing, we analyzed the structural and functional characteristics of the gut bacterial community in adults under heat stress. …”
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Huanglian Ejiao Decoction Alleviates Ulcerative Colitis in Mice Through Regulating the Gut Microbiota and Inhibiting the Ratio of Th1 and Th2 Cells by Tang J, Hu Y, Fang J, Zhu W, Xu W, Yu D, Zheng Z, Zhou Q, Fu H, Zhang W

“…The therapeutic efficacy of HED on UC was assessed by evaluating survival rate and so on. Flow cytometry was employed to assay the percentages of CD4+T cell. …”
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Early re-examination of chest CT may be unnecessary when patients with psittaci pneumonia at low and intermediate risk on Pneumonia Severity Index present with response to initial... by Ming Chen, Shu Ying Zeng, Sheng Jia Lu, Miao Shi, Qing Qian Zhang

“…With the development of metagenomic second-generation sequencing technology (mNGS), its diagnostic rate has improved in recent years. …”
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Success of Immunopathogenetic Therapy for Management of Congenital Ichthyosis, Combined Form, in a Child: Rare Clinical Case by Karine O. Avetisyan, Nikolay N. Murashkin, Ekaterina S. Pavlova, Roman A. Ivanov, Svetlana V. Petrichuk, Daria G. Kuptsova, Dmitrii S. Demyanov, Kirill V. Savostyanov

“…Molecular genetic study conducted via high-throughput sequencing followed by validation with Sanger sequencing has revealed two genetic variants: novel variant chr18:28934543G&gt;T in the DSG1 gene in the heterozygous state and pathogenic variant chr5:157468728C&gt;A in the NIPAL4 gene in the homozygous state. …”
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Distal electrical stimulation enhances neuromuscular reinnervation and satellite cell differentiation for functional recovery by Chun-Wei Lin, Szu-Han Chen, Siao Muk Cheng, Tzu-Chun Chung, Wentai Liu, Daw-Yang Hwang, Song Li, Sheng-Che Lin, Yuan-Yu Hsueh

“…Additionally, single-cell RNA sequencing was performed to examine gene expression in Satellite cells. …”
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Single-Cell Analysis Reveals Aspirin Restores Intervertebral Disc Integrity via Ferroptosis Regulation by Niu H, Qi H, Zhang P, Meng H, Liu N, Zhang D

“…Disc degeneration involves complex processes such as inflammation, matrix degradation, and cell death, yet the underlying mechanisms remain poorly understood. Single-cell RNA sequencing offers a powerful approach to elucidate cellular heterogeneity and dynamic changes in IVDD, providing valuable insights for early diagnosis and targeted therapeutic strategies.Methods: The Harmony algorithm was used to integrate four independent single-cell sequencing datasets. …”
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PD-1 regulates the anti-tumor immune function of macrophages through JAK2-STAT3 signaling pathway in colorectal cancer tumor microenvironment by Han Jiang, Jingjing Pang, Tengyue Li, Atitso Akofala, Xiaoxi Zhou, Changhua Yi, Shangwei Ning, Xu Gao, Yu Qiao, Jiayuan Kou

“…Methods In this study, we combined bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) data to investigate the association between PD-1 expression on macrophages and patient prognosis, while also uncovering the molecular mechanisms by which PD-1 regulates macrophage function. …”
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Clinical and functional characterization of the GABRB3 p.Met80Val variant in early-onset epilepsy with long-term follow-up by Ran Hua, Junhong Jiang, Baotian Wang, Zihao Zhu, Juan Wang, De Wu, Li Yang

“…Methods Whole-exome sequencing, validation of the identified GABRB3 variants using Sanger sequencing, and structural modeling were done to assess the potential impact of these variants on receptor function. …”
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High Humidity Alters Myeloid-Derived Suppressor Cells in Spleen Tissue: Insights into Rheumatoid Arthritis Progression by Wang H, Wang M, Wang T, Li X, Wen C, He Z, Huang L

“…The arthritis status of the mice was evaluated under two distinct humidity conditions (50% and 80%). …”
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A common mechanism of detoxification for lambda-cyhalothrin and abamectin in Cydia pomonella by Di Ju, Chao Hu, Peirong Li, Ping Gao, Yuting Li, Xueqing Yang

“…RNA-sequencing (RNA-Seq) was performed to compare the expression levels of detoxification-related genes between susceptible (SS) and resistant strains. …”
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Aberrant gene-specific DNA methylation signature analysis in cervical cancer by Samatha Bhat, Shama Prasada Kabekkodu, Vinay Koshy Varghese, Sanjiban Chakrabarty, Sandeep P Mallya, Harish Rotti, Deeksha Pandey, Pralhad Kushtagi, Kapaettu Satyamoorthy

“…In total, 5-methylcytosine, 5-hydroxymethylcytosine, and promoter methylation of 33 genes were evaluated by reversed-phase high-performance liquid chromatography, enzyme-linked immunosorbent assay based technique, and bisulfate-based next generation sequencing. …”
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Liposomal Nanoparticle Delivery of Ginkgo Flavone Glycosides Enhances SIRT1 Activation and Improves Diabetic Cardiomyopathy by Gao W, Chen R, Tong H, Wang Y, Liang L, Li Y, Yu H

“…In vitro experiments and proteomic sequencing were utilized to validate the regulatory effects of SIRT1.Results: The in vivo animal experiment results demonstrated that treatment with Ginkgo Flavone Glycosides (GFGs) significantly improved cardiac function in diabetic cardiomyopathy mice. …”
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