p53 Immunohistochemistry Patterns Are Surrogate Biomarkers for TP53 Mutations in Gastrointestinal Neuroendocrine Neoplasms by Junjie Li, Jing Wang, Dan Su, Xiu Nie, Yueping Liu, Lianghong Teng, Junyi Pang, Huanwen Wu, Zhiyong Liang

“…The cohort included 35 well-differentiated neuroendocrine tumors and 57 poorly differentiated neuroendocrine carcinomas. Gene sequencing revealed 38 wild-type TP53 and 54 TP53 mutations. p53 expression was interpreted as follows: pattern A, p53 was absent from all tumor cells; pattern B, scattered and weak p53 expression in 1-20% of tumor cells; and pattern C was subclassified as pattern C1: variable p53 staining intensity in 21-60% of tumor cells and tumor cell nests with focal strong positive p53 staining and pattern C2: strong p53 staining in more than 60% of tumor cells. p53 IHC patterns were evaluated as a binary classifier where pattern B predicted wild-type TP53, and patterns A and C predicted TP53 mutations. …”
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Influence of phragmites density, algal concentration and water velocity on cyanobacterial bloom dynamics by Jiaming Lv, Guijun Yang, Yuqing Zhang, Keqiang Shao, Xiangming Tang

“…Bacterial 16S rRNA gene sequencing was performed to assess diversity, and statistical analyses including α-diversity, β-diversity, and analysis of similarities (ANOSIM) were conducted to evaluate the impact of the experimental factors on water quality and bacterial community structures. …”
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Endothelial cell monolayer formation on a small-diameter vascular graft surface under pulsatile flow conditions by M. Yu. Khanova, E. A. Velikanova, V. G. Matveeva, E. O. Krivkina, T. V. Glushkova, V. V. Sevostianova, A. G. Kutikhin, L. V. Antonova

“…Objective: to create a cell-populated small-diameter vascular graft (SDVG) using autologous endothelial cells and extracellular matrix proteins, and to evaluate the efficiency of endothelial cell monolayer formation during shear stress preconditioning in a SDVG.Materials and methods. …”
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Metabolic profiling of glioblastoma and identification of G0S2 as a metabolic target by Jianlei Kang, Yujie Xu, Qitai Zhao, Ying Wang, Zhenyan He, Xin Xu

“…Finally, we knocked out G0S2 in glioma cells and performed RNA sequencing to investigate differentially activated pathways. …”
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The Comprehensive Adaptive Multisite Prevention of University Student Suicide Trial: Protocol for a Randomized Controlled Trial by Kyla Blalock, Jacqueline Pistorello, Shireen L Rizvi, John R Seeley, Francesca Kassing, James Sinclair, Linda A Oshin, Robert J Gallop, Cassidy M Fry, Ted Snyderman, David A Jobes, Jennifer Crumlish, Hannah R Krall, Susan Stadelman, Filiz Gözenman-Sapin, Kate Davies, David Steele, David B Goldston, Scott N Compton

“…ObjectiveThe Comprehensive Adaptive Multisite Prevention of University Student Suicide (CAMPUS) Trial evaluates 4 treatment sequences within UCCs to develop evidence-based treatment guidelines. …”
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Narirutin mitigates inflammatory arthritis and osteoporosis through modulating macrophage phenotype and osteoclastogenesis by Qing Wang, Xiaole Peng, Hao Xu, Yuhu Zhao, Xiaoheng Lu, Chengyao Lu, Qihan Wang, Wei Lu, Qifeng Sheng, Xiaomin Lu, Yaozeng Xu, Dechun Geng

“…This study aimed to evaluate the therapeutic potential of NRT in the context of inflammatory arthritis and osteoporosis. …”
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Identification and validation of BATF as a prognostic biomarker and regulator of immune cell infiltration in acute myeloid leukemia by Zhe Zhao, Dongmei Wang, Dongmei Wang, Xue Sheng, Shuying Li, Tingting Liu, Mengyuan Chang, Lei Feng, Di Zhang, Chunyan Ji, Chunyan Ji, Fei Lu, Jingjing Ye

“…In the meantime, the prognostic value of BATF was evaluated using univariate and multivariate analyses, receiver operating characteristic (ROC) curve (AUC) analysis, and Kaplan-Meier (KM) survival analysis. …”
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Differential Adjuvant Activity by Flagellins from <i>Escherichia coli</i>, <i>Salmonella enterica</i> Serotype Typhimurium, and <i>Pseudomonas aeruginosa</i> by Shengmei Pang, Mei Liu, Longlong Wang, Mingqing Shao, Guoqiang Zhu, Qiangde Duan

“…This study aims to analyze the amino acid sequences and structural features of flagellins from <i>Escherichia coli</i> (FliC<i>_E.C</i>), <i>Salmonella enterica</i> serotype Typhimurium (FliC<i>_S.T</i>), and <i>Pseudomonas aeruginosa</i> (FliC<i>_P.A</i>), and to evaluate their adjuvant activities in terms of Toll-like receptor 5 (TLR5) activation, antibody production, and cytokine responses in a murine model. (2) <b>Methods</b>: Bioinformatics analysis was conducted to compare the amino acid sequences and structural domains (D0, D1, D2, and D3) of flagellins from the three bacterial species. …”
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Beneficial Soil Fungi Isolated from Tropical Fruit Crop Systems for Enhancing Yield and Growth in Dragon Fruit in Ecuador by Yoansy Garcia, Danilo Valdez, Daniel Ponce de Leon, Hypatia Urjilez, Jaime Santos-Pinargote, Daniel Mancero-Castillo

“…This study aimed to identify native beneficial soil fungi associated with dragon fruit cultivation on the Ecuadorian coast and evaluate their effect on commercial production. Fungal isolates from four dragon fruit plantations were identified using microscopy and genetic sequencing (ITS, EF-1α, and beta-tubulin). …”
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Comparison of imaging markers of nerve ultrasound and MR-neurography in a longitudinal course in chronic inflammatory demyelinating polyneuropathy by Benjamin Lüling, Fabian Preisner, Jeremias Motte, Anna Lena Fisse, Thomas Grüter, Rafael Klimas, Emelie Schäfer, Annika Altenborg, Devrim Colak, Jörg Philipps, Tim Godel, Daniel Schwarz, Sabine Heiland, Min-Suk Yoon, Ralf Gold, Martin Bendszus, Moritz Kronlage, Kalliopi Pitarokoili

“…Methods and design: We longitudinally examined 12 CIDP patients from 2016 to 2022 using NUS, MRN, nerve conduction studies (NCS), and clinical parameters (inflammatory neuropathy cause and treatment (INCAT)/overall disability sum score (ODSS)). NUS evaluated the cross-sectional area (CSA) of the median, ulnar, radial, tibial, fibular, and sural nerve as well as the intranerve CSA variability (INV csa ) of the tibial, fibular, ulnar, and median nerve, whereas MRN evaluated T2-weighted sequences of the fibular and tibial nerve at the popliteal fossa. …”
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Construction and verification of a prognostic model for bladder cancer based on disulfidptosis-related angiogenesis genes by Zhihao Zhou, Yuwei Zhang, Yuhua Zhou, Jiayu Gu, Jufa Li, Jianfeng Shao, Ninghan Feng

“…Methods This study used sequencing data of BLCA in the Cancer Genome Atlas (TCGA) database. …”
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Association Between Oral Microbiota Dysbiosis and the Risk of Dementia: A Systematic Review by Alain Manuel Chaple-Gil, Meylin Santiesteban-Velázquez, Joaquín Juan Urbizo Vélez

“…<b>Methods</b>: Eligible studies evaluated oral microbial composition using validated methods such as genetic sequencing, bacterial culture, or metagenomic analysis. …”
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Identification of PRKCQ-AS1 as a Keratinocyte-Derived Exosomal lncRNA That Promotes Th17 Differentiation and IL-17&amp;nbsp;secretion in Psoriasis Through Bioinformatics, Machine L... by Gao P, Gao X, Lin L, Zhang M, Luo D, Chen C, Li Y, He Y, Liu X, Shi C, Yang R

“…This study investigates key exosomal ncRNAs regulating the Th17/IL-17 axis in psoriasis and their mechanisms.Methods: We integrated bulk RNA sequencing datasets from the GEO database to construct and evaluate exosome-related patterns. …”
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Hyperuricemia suppresses lumican, exacerbating adverse remodeling after myocardial infarction by promoting fibroblast phenotype transition by Zehao Zhuang, Ao Liu, Jinghong Zhang, Shuangjian Han, Lu Tang, Tingting Yu, Yiping Shi, Hui Li, Heng Yang, Peiyuan Bai, Yanhua Tang

“…Objectives We aimed to trigger hyperuricemia by administering potassium oxonate in a mouse model of MI to evaluate the role of hyperuricemia in MI pathogenesis. …”
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Veillonella parvula outer membrane vesicles increase ICAM-1+ neutrophils exhibiting elevated NET formation via ROS–PAD4 signaling by Lina Xu, Yiting Jiang, Xuri Zhao, Jiabao Zhuang, Huiwen Chen, Teng Li, Zhiyan He, Wei Zhou, Zhongchen Song

“…No significant differences were observed in neutrophil phagocytosis. RNA sequencing analysis revealed a significant increase in the expression of Icam-1 mRNA. …”
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Gut microbiome and metabolome characteristics of patients with cholesterol gallstones suggest the preventive potential of prebiotics by Ye Liu, Hexin Li, Tianhan Sun, Gaoyuan Sun, Boyue Jiang, Meilan Liu, Qing Wang, Tong Li, Jianfu Cao, Li Zhao, Fei Xiao, Fangqing Zhao, Hongyuan Cui

“…Microbiome taxonomic profiling was conducted on 191 samples via V3−V4 16S rRNA sequencing. Next, 60 samples (30 age‐ and sex‐matched CGS patients and 30 controls) were selected for metagenomic sequencing and fecal metabolite profiling via liquid chromatography‐mass spectrometry. …”
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Therapeutic role of aripiprazole in cartilage defects explored through a drug repurposing approach by Jun-Ku Lee, Hyunjeong Yeo, Sujin Choi, Kyeong Mi Kim, Hannah Kim, Sung-Sahn Lee, Hyun Il Lee, Younghoon Jeong, Hyun-Ju An, Soonchul Lee

“…KEGG pathway and Gene Ontology Biological Processes (GOBP) analyses of the mRNA sequencing data revealed that aripiprazole upregulated genes related to ribosomes and cytoplasmic translation, thereby facilitating chondrogenesis. …”
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Occurrence and genetic diversity of the zoonotic rat hepatitis E virus in small mammal species, Spain by Javier Caballero-Gómez, Tomás Fajardo-Alonso, Lucia Rios-Muñoz, Raúl Cuadrado-Matías, Aitor Somoano, Rosario Panadero, María Casares-Jiménez, Ignacio García-Bocanegra, Laura Ruiz, Adrián Beato-Benítez, Francisco Ruiz-Fons, Débora Jiménez-Martín, Alberto Espí, Ana del Cerro, Remigio Martínez, Mario Frías, Antonio Rivero-Juárez, Antonio Rivero

“…The presence of viral RNA was evaluated for ratHEV-C1 infection using RT-qPCRs in liver tissue. …”
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Impact of <i>Ectropis grisescens</i> Warren (Lepidoptera: Geometridae) Infestation on the Tea Plant Rhizosphere Microbiome and Its Potential for Enhanced Biocontrol and Plant Healt... by He Liu, Wei Chen, Xiaohong Fang, Dongliang Li, Yulin Xiong, Wei Xie, Qiulian Chen, Yingying You, Chenchen Lin, Zhong Wang, Jizhou Wang, Danni Chen, Yanyan Li, Pumo Cai, Chuanpeng Nie, Yongcong Hong

“…Additionally, bacterial feedback assays were conducted to evaluate the effects of selected microbial strains on plant growth and pest defense responses. …”
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Impacts of phosphate-solubilizing bacterium strain MWP-1 on vegetation growth, soil characteristics, and microbial communities in the Muli coal mining area, China by Yanru Wu, Yanru Wu, Wenquan Yang, Jiancun Kou, Jiancun Kou, Qinyao Li, Jiaqing Liu, Lu Chi, Yangcan Zhang, Qian Liu, Yanghua Yu

“…Their poor nutrient content may be ameliorated by introducing specific microorganisms into the soil. This study aims to evaluate the effects of a highly efficient phosphate solubilizing bacterium MWP-1, Pseudomonas poae, on plant growth, soil nutrients in remedying the soil of the high-altitude Muli mining area in Qinghai Province, and analyze its impact on microbial communities through high-throughput sequencing soil microbial communities. …”
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