Characterization of two novel chia (Salvia hispanica L.) white and black genotypes via DNA barcoding, physiological, and agronomic traits by Clara R. Azzam, Mokhtar Said Rizk, Soha Sayed Mohammad Mostafa, Ramadan A. Arafa, Mohamed Samy Al-Nabawi, Nahid Abdelaty Ali Morsi, Marwa Mahmoud Nasr El-Din, Enass Hassan Taher, Khaled Adly Khaled

“…Genetic diversity was evaluated through phylogenetic analysis with appropriate bioinformatics tools. …”
Get full text

IGF2BP3 Modulates mRNA Splicing and Stability to Promote Trophoblast Progression via Interaction with PDE3A and Suppression by miR-196a-5p in Preeclampsia by Chunyan Li, Pingpo Ming, Cuifang Fan, Jiao Chen, Jing Yang

“…We performed transcriptome sequencing of seven PE and seven normal placentas to identify differentially spliced events. …”
Get full text

Application of Intravoxel Incoherent Motion in the Prediction of Intra-Tumoral Tertiary Lymphoid Structures in Hepatocellular Carcinoma by Ma L, Liao S, Zhang X, Zhou F, Geng Z, Hu J, Zhang Y, Zhang C, Meng T, Wang S, Xie C

“…The recurrence-free survival (RFS) was evaluated with Kaplan–Meier curves.Results: A total of 168 patients were divided into training (n=128) and testing (n=40) cohorts (mean age: 56.83± 14.43 years; 149 [88.69%] males; 130 TLSs+). …”
Get full text

A validated heart-specific model for splice-disrupting variants in childhood heart disease by Robert Lesurf, Jeroen Breckpot, Jade Bouwmeester, Nour Hanafi, Anjali Jain, Yijing Liang, Tanya Papaz, Jane Lougheed, Tapas Mondal, Mahmoud Alsalehi, Luis Altamirano-Diaz, Erwin Oechslin, Enrique Audain, Gregor Dombrowsky, Alex V. Postma, Odilia I. Woudstra, Berto J. Bouma, Marc-Phillip Hitz, Connie R. Bezzina, Gillian M. Blue, David S. Winlaw, Seema Mital

“…Methods We used genome sequencing (GS) (n = 1101 CHD probands) and myocardial RNA-Sequencing (RNA-Seq) (n = 154 CHD and n = 43 cardiomyopathy probands) to identify and validate splice disrupting variants, and to develop a heart-specific model for canonical and non-canonical splice variants that can be applied to patients with CHD and cardiomyopathy. …”
Get full text

Bacteroides vesicles promote functional alterations in the gut microbiota composition by Olga Yu. Shagaleeva, Daria A. Kashatnikova, Dmitry A. Kardonsky, Boris A. Efimov, Viktor A. Ivanov, Svetlana V. Smirnova, Suleiman S. Evsiev, Eugene A. Zubkov, Olga V. Abramova, Yana A. Zorkina, Anna Y. Morozova, Elizaveta A. Vorobeva, Artemiy S. Silantiev, Irina V. Kolesnikova, Maria I. Markelova, Evgenii I. Olekhnovich, Maxim D. Morozov, Polina Y. Zoruk, Daria I. Boldyreva, Victoriia D. Kazakova, Anna A. Vanyushkina, Andrei V. Chaplin, Tatiana V. Grigoryeva, Natalya B. Zakharzhevskaya

“…We also analyzed the microbiota composition using 16S rRNA gene sequencing. Finally, we evaluated the volatile compound composition in the animals’ stools by HS-GC/MS to assess the functional activity of the microbiota. …”
Get full text

Evolution and characteristics of tetracycline resistance in Mycoplasma pneumoniae by Panpan Xie, Jie Liu, Yuzhong Feng, Taiyun Zhao, Wenkai Niu, Yun Fang, Xin Zhang, Xilin Zhao, Fusheng Wang, Xin Yuan

“…The whole genomes of the induced resistant and parent strains were both sequenced to identify tetracycline-resistance genes, and their 16S rRNA gene sequences were compared. …”
Get full text

MMRN1 Facilitates Renal Cell Carcinoma by Activating AMPK/MMPs Axis by Mingji Ye, Jian Cao, Zhihao Ming, Yu Xie

“…The present study aimed to explore the role of MMRN1 in renal cell carcinoma (RCC) and its related molecular mechanisms. Methods RNA sequencing was used to detect differential gene expression in RCC. …”
Get full text

Assessment and Monitoring of the Wound Micro-Environment in Chronic Wounds Using Standardized Wound Swabbing for Individualized Diagnostics and Targeted Interventions by Julian-Dario Rembe, Waseem Garabet, Jan-Wilm Lackmann, Sadaf Alizadehrahrouei, Matthias Augustin, Joachim Dissemond, Wiebke Ibing, Karl Köhrer, Klaus Pfeffer, Anna Rommerskirchen, Sebastian Alexander Scharf, Tobias Wienemann, Thorsten Wachtmeister, Hubert Schelzig, Ewa Klara Stuermer

“…A sample collection and handling protocol suitable for everyday use was tested and evaluated regarding feasibility for multiplex immunoassay, proteomics, small RNA sequencing (miRNA) and metagenome analyses (microbiomics). …”
Get full text

HPV16 genome structure analysis in oropharyngeal cancer PDXs identifies tumors with integrated and episomal genomes by Claire D. James, Raymonde O. Otoa, Aya H. Youssef, Christian T. Fontan, Malay K. Sannigrahi, Brad Windle, Devraj Basu, Iain M. Morgan

“…Thus, there is need for OPC preclinical models to evaluate standard and experimental therapeutics in the presence of episomal HPV16 oncogenic drivers. …”
Get full text

A Comparative Study of the Antioxidant and Antidiabetic Properties of Fermented Camel (<i>Camelus dromedarius</i>) and Gir Cow (<i>Bos primigenius indicus</i>) Milk and the Product... by Brijesh Bhuva, Bethsheba Basaiawmoit, Amar A. Sakure, Pooja M. Mankad, Anita Rawat, Mahendra Bishnoi, Kanthi Kiran Kondepudi, Ashish Patel, Preetam Sarkar, Subrota Hati

“…To optimize the growth conditions, the level of proteolysis was evaluated by exploring various inoculation levels (1.5, 2.0 and 2.5%) as well as incubation durations (0, 12, 24, 36 and 48 h). …”
Get full text

Effects of intermittent theta burst stimulation on the inflammatory response and cerebral blood flow in promoting neurovascular repair after ischemic stroke by Jingjun Zhang, Siyue Li, Dan Huang, Jiale Fu, Shuying Chen, Na Ren, Pengkun Yang, Di Song, Xiaochen Bai, Hongyu Xie, Gang Liu, Kewei Yu, Shamay S. M. Ng, Junfa Wu, Xiao Xiao, Yi Wu

“…Behavioral assessments were conducted to evaluate motor function recovery. The impact of iTBS on neuronal damage and apoptosis in the peri-infarct area was evaluated via Nissl staining and a TUNEL assay. …”
Get full text

Utilizing Viral Metagenomics to Characterize Pathogenic and Commensal Viruses in Pediatric Patients with Febrile Neutropenia by Anielly Sarana da Silva, Gabriel Montenegro de Campos, Gabriela Marengone Altizani, Enéas de Carvalho, Alice Chagas Barros, Eleonora Cella, Simone Kashima, Sandra Coccuzzo Sampaio, Maria Carolina Elias, Marta Giovanetti, Carlos Alberto Scrideli, Svetoslav Nanev Slavov

“…Metagenomic sequencing was performed on 15 pediatric patients with oncological diseases and FN at the outpatient clinic of Pediatric Oncology at the University Hospital of the Faculty of Medicine of Ribeirão Preto, University of São Paulo. …”
Get full text

Effects of fermented Arctium lappa L. root by Lactobacillus casei on hyperlipidemic mice by MingJu Chen, Yuxiao Wu, Hongxuan Yang, Tianfeng Liu, Tongkun Han, Wangqiang Dai, Junyue Cen, Fan Ouyang, Jingjing Chen, Jianxin Liu, Lin Zhou, Xuguang Hu

“…In vitro antioxidant activity, liver injury, lipid levels, pro-inflammatory cytokine levels, and cholesterol-related mRNA expression were assessed. 16S rRNA sequencing was conducted to evaluate changes in gut microbiota composition.ResultsLC-AR exhibited stronger antioxidant activity and higher metabolite levels than AR. …”
Get full text

Torsional capacity of prestressed assembled quarter-shell concrete tower with adhesive bonded vertical joints by Wei-Feng Tao, Dorjee TseDan, Dong-Liang Zhang, Ci-Rong Huang, Kun Fu, Chu Zhao, Jia-Wei Zhang, Jun-Lin Li, Zhi-Qiang Wan

“…The constrained torsion model can be employed to evaluate the torsional capacity of whole ring towers quite well, but does not apply to quarter-shell towers. …”
Get full text

Upper tract urothelial cancer (UTUC) genomic profiling and correlation regarding benefit of platinum-based chemotherapy by Min Woo Hwang, Jasmine Kauffeld, Sarah Belay, Joep J. de Jong, Elai Davicioni, Wenping Li, Jeanny B. Aragon-Ching

“…Molecular markers stratifying urothelial tumors as luminal subtype and non-luminal subtype tumors have been proposed to select patients who may have greater or lesser benefit from neoadjuvant systemic therapy in bladder cancer, though not yet evaluated in UTUC. Here, a single-institution study retrospectively obtained clinical and genomic information in patients with UTUC and evaluated four patient tumors using the Decipher Bladder® assay and Foundation Medicine® test. …”
Get full text

Bojungikki-Tang Augments Pembrolizumab Efficacy in Human PBMC-Injected H460 Tumor-Bearing Mice by Se Won Na, Jin-Mu Yi, Heerim Yeo, Sang-Min Park, Mibae Jeong, Jaemoo Chun, Mi-Kyung Jeong

“…Both agents were administered to evaluate their impact on tumor growth and immune cell behavior. …”
Get full text

Feasibility of RNA and DNA Extraction from Fresh Pipelle and Archival Endometrial Tissues for Use in Gene Expression and SNP Arrays by Heather D. Kissel, Thomas G. Paulson, Karen Liu, Xiaohong Li, Elizabeth Swisher, Rochelle Garcia, Carissa A. Sanchez, Brian J. Reid, Susan D. Reed, Jennifer Anne Doherty

“…FFPE-derived DNA should be evaluated on new rapidly evolving sequencing platforms.…”
Get full text

Milling degree affects the fermentation properties of rice: perspectives from the composition of nutrients and gut microbiota via in vitro fermentation by Yu Zhang, Fan Li, Shutong Pan, Bing Bai, Kai Huang, Sen Li, Hongwei Cao, Tian Xie, Jian Xie, Xiao Guan

“…Effects of fermentation substrates of rice with different MDs on gut microbiota were evaluated by 16S rDNA sequencing. All the sample groups reduced the pH and produced short-chain fatty acids (SCFAs) and branched-chain fatty acids. …”
Get full text

Exploratory analysis of molecular traits of the mitochondrial DNA of leafcutting ants to infer taxonomic characters towards an integrative taxonomy by Perpetua Ndubuisi Onyinyechi, Natália Martins Travenzoli, Maykon Passos Cristiano, Danon Clemes Cardoso

“…In the present study we propose using exploratory analysis of genetic markers to obtain additional information from the comparison of sequences among species as molecular traits (MTs). …”
Get full text

Elaboración y evaluación de una webquest como actividad integradora en las competen- cias básicas de educación secundaria en la enseñanza del FLE by Mª José Arévalo

“…Ce projet nous permet, donc, d’envisager comment construire des séquences didactiques significatives pour intégrer le numérique et les compétences dans l’enseignement-apprentissage du FLE. …”
Get full text